• 복식
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• 제24권
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• pp.217-233
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• 1995

The ultimate purpose of this study is to suggest the most effective marketing strategy for the clothing consumer market based on the new generation consumer's clothing selection behavior analysis. In this thesis, it is appempted to make a progress in the new gen-eration consumer's clothing life style types, in clothing purchase behavior analysis among the clothing life style, and also in the marketing strategy for marketers. The subjects selected for the final analysis are 412 the new gerneration women of age 20 thru 34 in seoul and satellite town area. Data were processed the spss package program. As for the analytic method, factor analysis, clustering analysis, XCross-tubulation, F-test with ANOVA, frequency and percentage were applied in the survey. The major findings are as following : life style is classified into four types : The characteristic fashion-directory type(25.7%) ; The reason traditional type(9.0%) ; The sen-sitivity fashion-following type(11.0%) ; The community brand-conscious type(54.3%). 2 Clothing life style types characteristic of the new generation consumer proved that clothing life style types are a significant difference according to the life style, the fashion consciousness and the average monthly spend-ing on clothing. 3. There is an important discrimination according to the clothing life style types in their clothing purchase behavior such as infor-mation usage, clothing choice criterion and brand loyalty. 4. Based on the result of our analysis and the review of literature, the marketing strategy is suggested that characteristic and new design development is efficient way to consumer's purchase need. Therefore apparel industary which pursue an added value must frame marketing strategy on the basis of the target consumer's sensitivity characteristic according to the life style and fashion consciousness.

• ALGAE
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• 제31권4호
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• pp.391-401
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• 2016

To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only $1-3{\times}10^5cells$ of P. minimum and A. minutum, $10^4cells$ of P. micans, and $10^3cells$ of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.

• 한국축산식품학회지
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• 제37권3호
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• pp.464-468
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• 2017

The identification of pork in commercially processed meats is one of the most crucial issues in the food industry because of religious food ethics, medical purposes, and intentional adulteration to decrease production cost. This study therefore aimed to develop a method for the detection of pork adulteration in meat products using primers specific for pig mitochondrial DNA. Mitochondrial DNA sequences for pig, cattle, chicken, and sheep were obtained from GenBank and aligned. The 294-bp mitochondrial DNA D-loop region was selected as the pig target DNA sequence and appropriate primers were designed using the MUSCLE program. To evaluate primer sensitivity, pork-beef-chicken mixtures were prepared as follows: i) 0% pork-50% beef-50% chicken, ii) 1% pork-49.5% beef-49.5% chicken, iii) 2% pork-49% beef-49% chicken, iv) 5% pork-47.5% beef-47.5% chicken, v) 10% pork-45% beef-45% chicken, and vi) 100% pork-0% beef-0% chicken. In addition, a total of 35 commercially packaged products, including patties, nuggets, meatballs, and sausages containing processed chicken, beef, or a mixture of various meats, were purchased from commercial markets. The primers developed in our study were able to detect as little as 1% pork in the heat treated pork-beef-chicken mixtures. Of the 35 processed products, three samples were pork positive despite being labeled as beef or chicken only or as a beef-chicken mix. These results indicate that the developed primers could be used to detect pork adulteration in various processed meat products for application in safeguarding religious food ethics, detecting allergens, and preventing food adulteration.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2006년도 공동학술대회 논문집
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• pp.161-166
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• 2006

수직 중력 변화율 탐사는 중력 탐사에 비하여 분해능과 탐지능이 높고 광역 효과를 억제하는 이점이 있다. 수직 변화율의 실제 측정은 어려움이 많아 정확성과 실용성에서 의문이 제기되기도 하였으나, 현대적인 중력계로는 요구되는 정확도를 유지하는 것이 크게 어려운 일이 아니다. 특히, 얕은 깊이의 소규모 구조를 대상으로 하는 토목, 환경 문제에는 효과적으로 적용될 수 있다. 이 논문은 수직 중력 변화율 탐사를 천부 고분해 밀도 구조 해석에 적용하기 위하여 수직 중력 변화율에 관한 전반적 고찰과 수치 역산 모델링을 통하여 중력 탐사와 비교, 분석하였으며, 전남 무안의 석회암 공동 지역에서 수행한 수직 중력 변화율 탐사를 수행하고 역산, 해석하였다.

• 지질공학
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• 제21권1호
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• pp.87-95
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• 2011

이 연구는 신월성 원자력발전소 1,2호기(이하 신월성원전) 운영과 함께 국내 원자력관련 이용시설 부지(이하 원자력부지)에 처음 도입될 단층감시시스템의 설치 운영에 필요한 안전요건(즉, 규제 요건, 지침 및 기술기준)을 제공할 목적으로 국내외 단층감시시스템과 그와 유사한 측지시스템의 구축 사례를 수집, 분석하였으며, 단층감시시스템 구축 시 기본적으로 고려하여야 할 기술 사항들을 제시하였다. 단층감시시스템 구축 시 고려해야할 사항들로는 1) 부지고유특성의 이해(잡음 특성 포함), 2) 감시대상에 맞는 설비/설계 선택, 3) 백업시스템 구축, 4) 정례화된 검 교정체제를 통한 계측자료의 품질과 신뢰성 확보, 5) 투명한 운영체계와 정보공개 정책, 6) 장기적으로 안정적인 운영기반 등이 제안되었다.

• 비파괴검사학회지
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• 제21권5호
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• pp.542-548
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• 2001

과전류 보호계전기에 사용할 목적으로 광섬유를 센서헤드로 사용하는 광 전류센서를 구성하였다. 전류측정의 원리는 도체 주변에 감겨진 광섬유 코일을 따라 광신호의 선형편광축이 자기장에 의하여 회전하는 정도를 측정하여 이로부터 전류의 값을 산출하는 편광측정 방법이 사용되었다. 센서코일은 $10/125{\mu}m$ 규격의 일반 통신용 광섬유를 미터당 20회전 이상 비틀어 제작하여 온도나 진동 등의 환경적 영향을 최소화하였다. 코일의 한쪽 끝은 Faraday Rotator Mirror를 장착한 반사형으로 설계하여 계통을 해체하지 않은 상태에서도 센서의 탈 부착이 가능하고 코일의 회전수에 따라 센서의 민감도 조절이 용이하며 폐회로형 구조이므로 인근 신호원에 의한 간섭을 차단할 수 있다. 편광 빛가르개 등을 이용한 광학 신호처리부를 구성하고 표준 전류원을 이용한 전류측정 실험을 수행한 결과로 이론적인 해석과 동일한 형태의 출력을 얻을 수 있었다. 본 논문에서는 구성한 광 전류센서의 이론적 모델을 기술하고 전류측정 실험의 결과와 이론치에 대하여 비교 분석한다.

• Biomolecules & Therapeutics
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• 제15권4호
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• pp.266-272
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• 2007

A rapid analytical method was developed to quantify very long-chain fatty acids (VLCFAs, C22:0, C24:0, C26:0) in human plasma with good sensitivity and specificity using tert-butyldimethylsilyl (TBDMS) derivatization and stable isotope GC-MS selective ion monitoring (GC-MS/SIM). Two-hundred and fifty ${\mu}L$ of plasma was fortified with deuterated stable isotope internal standards (d3-C22:0, d3-C24:0, d3-C26:0) and standard mixtures of chloroform and methanol, and then extracted with hexane and acetonitrile. To upper layer of liquid-liquid-extraction, N-(t-Butyldimethylsilyl)-N-methyltrifluoroacetamide was added and then heated to $60^{\circ}C$ for 30 min to produce the TBDMS derivatives. Derivatives of VLCFAs were analyzed by GC-MS/SIM. Calibration curves showed a linear relationship for the target compounds in the concentration range of $10^{-4}{\sim}2{\times}10^3\;{\mu}g/mL$ with the correlation coefficient ranging from 0.996 to 0.999. The limit of quantification for the plasma was $10^{-4}{\sim}2{\times}10^{-4}\;{\mu}g/mL$ (S/N=3). When applied to the plasma specimens of patients with peroxisomal disorder, X-linked adrenoleucodystropy (ALD, Mckusick 202370), the method clearly differentiated normal subjects from ALD patients. The C24:0/C22:0 and C26:0/C22:0 ratios were significantly elevated in the plasma of patients with X-linked ALD compared to normal subjects. The new developed method might be useful for a rapid and sensitive diagnosis of X-linked ALD and other peroxisomal disorders.

• 한국물환경학회지
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• 제32권6호
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• pp.670-699
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• 2016

In this study, 16 antibiotics were selected from among the top 30 veterinary antibiotics sold in South Korea in 2014, as well as from among the pharmaceuticals targeted by EPA method 1694, in order to review analytical methods for the detection of trace levels of antibiotics in environmental samples: surface water, soils, animal origin foods, and manures. LC-MS/MS was heavily used. In the chromatography for the detection of the selected antibiotics, the $C_{18}$ column was mostly used at the temperature of $30{\sim}40^{\circ}C$. Water and methanol/acetonitrile were commonly chosen as a nonpolar and a polar mobile phase, respectively. Gradient elution was applied to separate multiclass antibiotics. Volatile additives, such as formic acid, acetic acid, and ammonium acetate were mixed with the mobile phase to improve the ionization efficiency of analytes and the sensitivity in MS detection. Electrospray ionization (ESI) was widely used in the LC-MS/MS and positive ionization was preferred to determine the selected antibiotics. A protonated $[M+H]^+$ molecule was selected as a precursor ion, and its two transitions were analyzed, one for quantitative measurement and the other for confirmation. This study reviewed linearity of the calibration curve, recovery, repeatability, method detection limits (MDLs), and method quantification limits (MQLs) for each target compound used to validate the developed analytical methods.

• 한국축산식품학회지
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• 제30권4호
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• pp.590-596
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• 2010

Meat and meat products are a potential source of food-borne pathogens, including Staphylococcus aureus, Salmonella spp., Escherichia coli O157:H7, and Bacillus cereus. A sensitive and specific PCR assay for the detection of these pathogens in meat and meat products was developed in this study, as part of a broader effort to reduce the potential health hazards posed by these pathogens. Initially, PCR conditions were standardized with purified DNA. Under standard conditions, the detection level for PCR was as low as 10 pg of purified bacterial DNA. After overnight growth of bacteria in a broth medium, as few as $10^2$ CFU of bacteria were detected by PCR assay. The primers employed in the PCR assay were found to be highly specific for individual organisms, and evidenced no cross-reactivity with heterologous organisms. Additionally, the multiplex PCR assays also amplified some target genes from the four pathogens, and multiplex amplification was obtained from as little as 10 pg of DNA, thus illustrating the excellent specificity and high sensitivity of the assay. In conclusion, this PCR-based technique provides a sensitive and specific method for the detection of S. aureus, Salmonella spp., E. coli O157:H7, and B. cereus in meat and meat products.

• 전자공학회논문지
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• 제52권3호
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• pp.190-195
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• 2015

돌연변이 및 유전병의 원인이 되고 있는 유전자 단일염기 다형성(single nucleotide polymorphisms; SNPs) 검출은 조기진단, 치료 및 제약등 바이오관련 분야에서 매우 중요하다. SNPs 검출을 위한 방법은 다양하게 제시되고 있으나 상보적 DNA와 SNPs의 에너지 차이가 미세하여 SNPs 검출에는 많은 어려움이 존재한다. 본 논문에서는 SNPs를 검출하기 위하여 전하 검출형 전계효과 트랜지스터(field-effect transistors; FETs)를 이용하여 DNA가 가지고 있는 음전하 측정 방법으로 SNPs를 검출하였다. 상보적 DNA와 SNPs의 미세한 에너지 차이를 구분하기 위하여 타게트 DNA hybridization공정에서 드레인-소스 전극에 -0.3 V의 음전압을 인가하였다. 음전압 인가에 따라 DNA 자체 음전하와 센서 표면의 음전압의 전기적 반발력에 의해 센서에 검출되는 타게트 DNA hybridization 신호 크기는 감소하였으나 상보적 DNA와 SNPs의 신호 차는 1.7 mV에서 8.7 mV로 5배 이상 증가하여 검출되었다.