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Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract (생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가)

  • Lee, Jong-Du;Hyun, Ho Bong;Hyeon, Hyejin;Jang, Eunbi;Ko, Min-Hee;Yoon, Weon-Jong;Ham, Young Min;Jung, Yong-Hwan;Choi, Hwon;O, Eu Gene;Oh, Daeju
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.435-444
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    • 2022
  • Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 mg/L sucrose, and 2 mg/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.

A Study on the Effect of Improving Permeability by Injecting a Soil Remediation Agent in the In-situ Remediation Method Using Plasma Blasting, Pneumatic Fracturing, and Vacuum Suction Method (플라즈마 블라스팅, 공압파쇄, 진공추출이 활용된 지중 토양정화공법의 정화제 주입에 따른 투수성 개선 연구)

  • Geun-Chun Lee;Jae-Yong Song;Cha-Won Kang;Hyun-Shic Jang;Bo-An Jang;Yu-Chul Park
    • The Journal of Engineering Geology
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    • v.33 no.3
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    • pp.371-388
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    • 2023
  • A stratum with a complex composition and a distributed low-permeability soil layer is difficult to remediate quickly because the soil remediation does not proceed easily. For efficient purification, the permeability should be improved and the soil remediation agent (H2O2) should be injected into the contaminated section to make sufficient contact with the TPH (Total petroleum hydrocarbons). This study analyzed a method for crack formation and effective delivery of the soil remediation agent based on pneumatic fracturing, plasma blasting, and vacuum suction (the PPV method) and compared its improvement effect relative to chemical oxidation. A demonstration test confirmed the effective delivery of the soil remediation agent to a site contaminated with TPH. The injection amount and injection time were monitored to calculate the delivery characteristics and the range of influence, and electrical resistivity surveying qualitatively confirmed changes in the underground environment. Permeability tests also evaluated and compared the permeability changes for each method. The amount of soil remediation agent injected was increased by about 4.74 to 7.48 times in the experimental group (PPV method) compared with the control group (chemical oxidation); the PPV method allowed injection rates per unit time (L/min) about 5.00 to 7.54 times quicker than the control method. Electrical resistivity measurements assessed that in the PPV method, the diffusion of H2O22 and other fluids to the surface soil layer reduced the low resistivity change ratio: the horizontal change ratio between the injection well and the extraction well decreased the resistivity by about 1.12 to 2.38 times. Quantitative evaluation of hydraulic conductivity at the end of the test found that the control group had 21.1% of the original hydraulic conductivity and the experimental group retained 81.3% of the initial value, close to the initial permeability coefficient. Calculated radii of influence based on the survey results showed that the results of the PPV method were improved by 220% on average compared with those of the control group.

Scenario-Based Analysis on the Effects of Green Areas on the Improvement of Urban Thermal Environment (녹지 조성 시나리오에 따른 도시 열환경 개선 효과 분석)

  • Min, Jin-Kyu;Eum, Jeong-Hee;Sung, Uk-Je;Son, Jeong-Min;Kim, Ju-Eun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.50 no.6
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    • pp.1-14
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    • 2022
  • To alleviate the urban heat island phenomenon, this study aims to quantitatively analyze the effects of neighborhood green spaces on the improvement of the thermal environment based on detailed scenarios of five types of green spaces, including parks, pocket parks, parking lot greening, roadside planting, and rooftop-wall greening. The ENVI-met 4.4.6v model, a microclimate simulation program, was used to analyze the effects of green spaces. As a result, it was found that the air temperature decreased as the planting density of the park increased, but the thermal comfort index PET, which is the degree of heat sensation felt by humans, was not directly proportional to temperature. The establishment of a pocket park reduced air temperature up to a radius of 56m, while the range of temperature reduction increased by about 12.5% when three additional pocket parks were established at 250m intervals. Unlike the air temperature, PET was only affected in the vicinity of the planted area, so there was no significant difference in the thermal comfort of the surrounding environment due to the construction of pocket parks. Changing the surface pavement from asphalt to lawn blocks and implementing rooftop or wall greening did not directly act as solar shading but positively affected air temperature reduction; PET showed no significant difference. Roadside planting showed a higher air temperature reduction effect as the planting interval was narrower, but PET was not directly proportional to tree density. In the case of shrub planting under trees, it did not significantly affect the air temperature reduction but positively affected the improvement of thermal comfort. This study can outline strategies for constructing neighborhood green spaces to solve the urban heat island phenomena and establish detailed strategies for efficient thermal environment improvements.

Evaluation of Physical Properties and Material Characterization for Structural Frame at the Stained Glass Windows to Gongju Jeil Church of the Registered Cultural Heritage in Korea (국가등록문화재 공주제일교회 스테인드글라스 구조재의 재질특성과 물성 평가)

  • Bo Young Park;Hye Ri Yang;Chan Hee Lee
    • Economic and Environmental Geology
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    • v.56 no.1
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    • pp.103-114
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    • 2023
  • The Christian Museum of Gongju Jeil Church was first built in 1931 and was largely damaged during the Korean War, but the walls and chimneys have been preserved. This building has a high architectural values in that the chapel was reconstructed in 1956, and maintains its original form through repair of damaged parts rather than new construction. The stained glass windows were as installed in 1979 and has a great significance in the Dalle de Verre method using lump glass. However, some of the stained glass damaged partially, such as various cracks and splits, and vertical and horizontal cracks in the joint fillers of supporting the colored glass. As the structural materials of the stained glass window, an iron frame and cement mortar filled with it were used, and corrosion of iron, cracking of mortar and granular decomposition appear partially due to weathering. In the joint fillers, the content of Ca and S is very high, indicating that gypsum were used as admixtures, and the gypsums grow in a rhombohedral and forms a bundle, which is investigated to have undergone recrystallization. As a result of modeling the ultrasonic velocity at the joint fillers, the left and right windows at the entrance show relatively weak in the range of 800 to 1,600m/s, and the lower right corner of the altar window and the upper left corner of the center window were also 1,000 to 1,800m/s, showing relatively low physical properties. And gypsums produced during the neutralization of lime mortar were detected in the joint fillers and contaminants on the surface. Such salts may cause damage to the joint material due to freezing and thawing, so appropriate preventive conservation is required. Also, since various damage types are complexly appearing in stained glass window and joint filler, customized conservation treatment should be reviewed through clinical tests.

Arthroscopically-Assisted Reduction and Internal Fixation of Intra-Articular Fractures of the Lateral Tibial Plateau (관절면을 침범한 경골 외측 고평부 골절에 대한 관절경을 이용한 정복 및 내고정술)

  • Kim, Juhan;Kim, Dong Hwi;Lim, Jae-Hwan;Jang, Hyunwoong;Kim, Young Wook
    • Journal of the Korean Orthopaedic Association
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    • v.54 no.3
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    • pp.227-236
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    • 2019
  • Purpose: To evaluate the results of tibial lateral plateau fractures using arthroscopic-assisted reduction and internal fixation without cortical window or bone grafts. Materials and Methods: From March 2009 to March 2017, 27 patients with Schatzker type II tibial plateau fractures with articular depression and displacement over 5 mm on a computed tomography (CT) scan, who were treated with arthroscopic reduction and internal fixation and followed-up for at least 18 months, were enrolled in this study. Under arthroscopic guidance, the depressed fracture fragment was reduced using a freer and fixed with 5.0 or 6.5 mm cannulated screws through the inframeniscal portal without a cortical window or bone graft. The clinical and radiological results were evaluated using a Rasmussen system. Second look arthroscopy was performed in thirteen patients during the implant removal operation. Results: All fractures healed completely with a mean union time of 8.7 weeks (range from 8 to 12 weeks). Twenty four patients had good to excellent clinical results and 25 patients had good to excellent radiological results according to the Rasmussen classification. A well-healed articular surface with fibrocartilage was also found in 13 cases with second look arthroscopy. The 8 cases on CT scan at outpatient department follow-up showed bone union without bone grafting. Conclusion: Arthroscopic-assisted fixation of tibial lateral plateau fractures is a useful method without a cortical window or bone graft that produces good clinical results.

Manufacturing Method and Characteristics of the Dongrok(copper chloride) pigments (동록(염화동) 안료의 제조방법 및 특성에 관한 연구)

  • KANG Yeongseok;PARK Juhyun;MUN Seongwoo;HWANG Gahyun;KIM Myoungnam;LEE Sunmyung
    • Korean Journal of Heritage: History & Science
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    • v.56 no.2
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    • pp.148-169
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    • 2023
  • Hayeob pigment is known as one of the traditional dark green pigments, but the color, raw material, and manufacturing method have not been clearly identified. However, comparing the analysis results of the particle shape and constituent minerals of Hayeob pigments revealed through pigment analysis studies of colored cultural properties such as Dancheong, Gwaebul, and paintings, Hayeob pigments appear to be the same as Dongrok pigments produced by salt corrosion. Therefore, in order to restore Hayeob pigment, the manufacturing method of Dongrok pigment was studied based on the records of old literature. The Dongrok pigment manufacturing method confirmed in the old literature records is a natural corrosion method in which copper powder and a caustic are mixed and then left in a humid condition to corrode. Based on this, artificial corrosion using a corrosion tester was adopted to corrode the copper powder more efficiently, and an appropriate mixing ratio was selected by analyzing the state of corrosion products according to the mixing ratio of the caustic agent. In addition, the manufacturing method of Dongrok pigment was established by adding a salt removal process to remove residual caustic agents and a purification process to increase chroma during pigment coloring. The prepared Dongrok pigments have a bluish green or green color, show an elliptical particle shape and a form in which small particles are aggregated, and a porous surface is observed. The main constituent elements are copper(Cu) and chlorine(Cl), and the main constituent mineral is identified as atacamite [Cu2Cl(OH)3]. As a result of an accelerated weathering test to evaluate the stability of the prepared Dongrok pigments, it was found that the greenness partially decreased and the yellowness significantly increased as deterioration progressed. Before deterioration, the Dongrok pigments had lower yellowness compared to the Hayeob pigments of the old Dancheong, but after deterioration, yellowness increased significantly, and it was found to have a similar chromaticity range as Dancheong's Hayeob pigments. As a result, the prepared Dongrok pigments were confirmed to be similar to Dancheong's Hayeob pigments in terms of color as well as particle shape and constituent minerals.

Development of Seasonal Habitat Suitability Indices for the Todarodes Pacificus around South Korea Based on GOCI Data (GOCI 자료를 활용한 한국 연근해 살오징어의 계절별 서식적합지수 모델 개발)

  • Seonju Lee;Jong-Kuk Choi;Myung-Sook Park;Sang Woo Kim
    • Korean Journal of Remote Sensing
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    • v.39 no.6_2
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    • pp.1635-1650
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    • 2023
  • Under global warming, the steadily increasing sea surface temperature (SST) severely impacts marine ecosystems,such as the productivity decrease and change in marine species distribution. Recently, the catch of Todarodes Pacificus, one of South Korea's primary marine resources, has dramatically decreased. In this study, we analyze the marine environment that affects the formation of fishing grounds of Todarodes Pacificus and develop seasonal habitat suitability index (HSI) models based on various satellite data including Geostationary Ocean Color Imager (GOCI) data to continuously manage fisheries resources over Korean exclusive economic zone. About 83% of catches are found within the range of SST of 14.11-26.16℃,sea level height of 0.56-0.82 m, chlorophyll-a concentration of 0.31-1.52 mg m-3, and primary production of 580.96-1574.13 mg C m-2 day-1. The seasonal HSI models are developed using the Arithmetic Mean Model, which showed the best performance. Comparing the developed HSI value with the 2019 catch data, it is confirmed that the HSI model is valid because the fishing grounds are formed in different sea regions by season (East Sea in winter and Yellow Sea in summer) and the high HSI (> 0.6) concurrences to areas with the high catch. In addition, we identified the significant increasing trend in SST over study regions, which is highly related to the formation of fishing grounds of Todarodes Pacificus. We can expect the fishing grounds will be changed by accelerating ocean warming in the future. Continuous HSI monitoring is necessary to manage fisheries' spatial and temporal distribution.

High-Resolution Paleoproductivity Change in the Central Region of the Bering Sea Since the Last Glaciation (베링해 중부 지역의 마지막 빙하기 이후 고생산성의 고해상 변화)

  • Kim, Sung-Han;Khim, Boo-Keun;Shin, Hye-Sun;Uchida, Masao;Itaki, Takuya;Ohkushi, Kenichi
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.14 no.3
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    • pp.134-144
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    • 2009
  • Paleoproductivity changes in the central part of the Bering Sea since the last glacial period were reconstructed by analyzing opal and total organic carbon (TOC) content and their mass accumulation rate (MAR) in sediment core PC23A. Ages of the sediment were determined by both AMS $^{14}C$ dates using planktonic foraminifera and Last Appearance Datum of radiolaria (L. nipponica sakaii). The core-bottom age was calculated to reach back to 61,000 yr BP. and some of core-top was missing. Opal and TOC contents during the last glacial period varied in a range of 1-10% and 0.2-1.0%, and their average values are 5% and 0.7%, respectively. In contrast, during the last deglaciation, opal and TOC contents varied from 5 to 22% and from 0.8 to 1.2%, respectively, with increasing average values of 8% and 1.0%. Opal and TOC MAR were low ($1gcm^{-2}kyr^{-1}$, $0.2gcm^{-2}kyr^{-1}$) during the last glacial period, but they increased (>5 and >$1gcm^{-2}kyr^{-1}$) during the last deglaciation. High diatom productivity during the last deglaciation was most likely attributed to the elevated nutrient supply to the sea surface resulting from increased melt water input from the nearby land and enhanced Alaskan Stream injection from the south under the restricted sea-ice and warm condition during the rising sea level. On the contrary, low productivity during the last glacial period was mainly due to decreased Alaskan Stream injection during the low sea-level condition as well as to extensive development of sea ice under low-temperature seawater and cold environment.

Influence of Spring Warming in the Arctic-East Asia Region on the Arctic Oscillation and Dust Days in Korea Attributed to Dust Storms (북극-동아시아 지역의 봄철 온난화가 북극 진동-한국의 황사 사례일의 종관 기상에 미치는 영향 분석)

  • Ji-Sun Kim;Jae-Hee Cho;Hak-Sung Kim
    • Journal of the Korean earth science society
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    • v.45 no.2
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    • pp.121-135
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    • 2024
  • This study examined the influence of near-surface atmospheric warming in the Arctic-East Asia region during spring (March-May) from 1991 to 2020 on the synoptic-scale meteorology of dust storm-induced dust days in Seoul, Korea, in response to the Arctic Oscillation. Increased springtime warming in the Arctic-East Asia region correlated with a reduction of six days in the occurrence of dust storm-induced dust days in Seoul, Korea, along with a decline in the intensity of these days by -1.6 ㎍ m-3yr-1 in PM10 mass concentration. The declining number of dust storm-induced dust days in Korea during the 2010s was the result of synoptic-scale meteorological analysis, which showed increased high-pressure activity as indicated by the negative potential vorticity unit. Moreover, a distinct pattern emerged in the distribution of dust storm-induced dust days in Korea based on the Arctic Oscillation Index (AOI), showing an increase in negative AOI and a decrease in positive AOI. Although the northward shift of the polar jet weakened the southerly low-pressure system activity over Mongolia and northern China, a reinforced high-pressure system formed over the Chinese continent during dust-storm-induced dust days with a negative AOI. This resulted in both a decrease in the frequency of dust-storm-induced dust days and reduction in wind speeds, facilitating their transport from source regions to Korea. Conversely, on days with positive AOIs, an extensive warm and stagnant high-pressure system dominated mainland China, accompanied by further cooling of the northern segment of the polar jet. A notable decline in wind speed in the lower troposphere across the Mongolia-northern China-Korea region diminished the occurrence of dust storm-induced dust days and also weakened their long-range transport.

Evaluation of the Natural Vibration Modes and Structural Strength of WTIV Legs based on Seabed Penetration Depth (해상풍력발전기 설치 선박 레그의 해저면 관입 깊이에 따른 고유 진동 모드와 구조 강도 평가)

  • Myung-Su Yi;Kwang-Cheol Seo;Joo-Shin Park
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.30 no.1
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    • pp.127-134
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    • 2024
  • With the growth of offshore wind power generation market, the corresponding installation vessel market is also growing. It is anticipated that approximately 100 installation vessels will be required in the of shore wind power generation market by 2030. With a price range of 300 to 400 billion Korean won per vessel, this represents a high-value market compared to merchant vessels. Particularly, the demand for large installation vessels with a capacity of 11 MW or more is increasing. The rapid growth of the offshore wind power generation market in the Asia-Pacific region, centered around China, has led to several discussions on orders for operational installation vessels in this region. The seabed geology in the Asia-Pacific region is dominated by clay layers with low bearing capacity. Owing to these characteristics, during vessel operations, significant spudcan and leg penetration depths occur as the installation vessel rises and descends above the water surface. In this study, using penetration variables ranging from 3 to 21 m, the unique vibration period, structural safety of the legs, and conductivity safety index were assessed based on penetration depths. As the penetration depth increases, the natural vibration period and the moment length of the leg become shorter, increasing the margin of structural strength. It is safe against overturning moment at all angles of incidence, and the maximum value occurs at 270 degrees. The conditions reviewed through this study can be used as crucial data to determine the operation of the legs according to the penetration depth when developing operating procedures for WTIV in soft soil. In conclusion, accurately determining the safety of the leg structure according to the penetration depth is directly related to the safety of the WTIV.