Browse > Article
http://dx.doi.org/10.7732/kjpr.2022.35.4.435

Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract  

Lee, Jong-Du (Biodiversity Research Institutue, Jeju Technopark)
Hyun, Ho Bong (Biodiversity Research Institutue, Jeju Technopark)
Hyeon, Hyejin (Biodiversity Research Institutue, Jeju Technopark)
Jang, Eunbi (Biodiversity Research Institutue, Jeju Technopark)
Ko, Min-Hee (Biodiversity Research Institutue, Jeju Technopark)
Yoon, Weon-Jong (Biodiversity Research Institutue, Jeju Technopark)
Ham, Young Min (Biodiversity Research Institutue, Jeju Technopark)
Jung, Yong-Hwan (Biodiversity Research Institutue, Jeju Technopark)
Choi, Hwon (The Lotus Corporation Limited)
O, Eu Gene (The Lotus Corporation Limited)
Oh, Daeju (Biodiversity Research Institutue, Jeju Technopark)
Publication Information
Korean Journal of Plant Resources / v.35, no.4, 2022 , pp. 435-444 More about this Journal
Abstract
Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 mg/L sucrose, and 2 mg/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.
Keywords
Adventitious root culture; Bioreactor; Bioactive compounds; Endangered plant; Hibiscus hamabo;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Ahn, Y.H. 2003. Distribution of native Hibiscus hamabo and ecological characteristics of naturally inhabited areas in Jeju Island. Hort. Sci. Tech. 21:440-446 (in Korean).
2 Amiarouche, L., T. Stuchbury and S. Mattews. 1985. Comparisons of cultivar performance on different nutrient media in a routine method for potato micropropagation. Potato Res. 28:469-478.   DOI
3 Chang, C., H. Lee, T.Y. Park and H. Kim. 2005. Reconsideration of rare and endangered plant species in Korea based on the IUCN red list categories. J. Ecol. Environ. 28:305-320 (in Korean).
4 Folin, O. and V. Ciocalteu. 1927. On tyrosine and tryptophan determination in proteins. J. Biol. Chem. 73:627-650.   DOI
5 Georgiev, V., A. Slavov, I. Vasileva and A. Pavlov. 2018. Plant cell culture as emerging technology for production of active cosmetic ingredients. Eng. Life Sci. 18:779-798.   DOI
6 Hasan, N.A., S. Hussein and R. Ibrahim. 2014. Plant growth regulator effect on adventitious roots induction of Labisia pumila. Mal. J. Fund. Appl. Sci. 10:49-52.
7 Hearing, V.J. and M. Jimenez. 1987. Mammalian tyrosinase-the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19:1141-1147.   DOI
8 Ho, T.T., T.M.N. Ha, T.K.C. Nguyen and T.D. Le. 2021. Pilot-scale culture of adventitious root for the production of pharmacology active from medicinal plants: a mini review. Bio. Conf. 40:03003.   DOI
9 Hosoi, J., E. Abe, T. Suda and T. Kuroki. 1985. Regulation of melanin synthesis of B16 mouse melanoma cells by lα, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 48:1474-1478.
10 Kim, D.M., J.Y. Jung, H.K. Lee, Y.S. Kwon, J.H. Baek and I.S. Han. 2020. Cosmetic composition for skin improvement contatining Aloe vera adventitious root extract. KSBB Journal 35:235-243 (in Korean).   DOI
11 Kligman, D. 2000. Cosmeceuticals. Derma. Clinics 18:609-615.   DOI
12 Liu, Y. and D. Zhong. 1997. Petrology of high-pressure granulites from the eastern Himalayan syntaxis. J. Metamorphic Geol. 15:451-466.   DOI
13 Ko, H.M., T.K. Eom, K.C. Kim, C.J. Kim, J.G. Lee and J.S. Kim. 2018. Antioxidant effects and tyrosinase and elastase inhibitory activities of mountain ginseng adventitious roots extracts at different ethanol concentrations. Korean J. Agri. Sci. 45:499-508.   DOI
14 Kwon, N.H., T.K. Kim, S.J. Park, H.C. Kim and C.K. Song. 2016. Screening of antifungal activity on the coastal plants 5 species. Korean J. Org. Agric. 24:465-484 (in Korean).   DOI
15 Lee, E.J., S.Y. Park and K.Y. Paek. 2015a. Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors. Plant Cell Tiss. Org. Cult. 120:1-10.   DOI
16 Lee, G., A. Yeom, K.D. Won, C.M. Park, M.S. Joung, G.Y. Lee and C.S. Jeong. 2018. Evaluation of Alcea rosea L. callus extract as a natural cosmetic ingredient. J. Soc. Cosmet. Sci. Korea. 44:295-302.   DOI
17 Lee, K.J., Y. Park, J.Y. Kim, T.K. Jeong, K.S. Yun, K.Y. Paek and S.Y. Park. 2015b. Production of biomass and bioactive compounds from adventitious root cultures of Polygonum multiflorum using air-lift bioreactors. J. Plant Biotechnol. 42:34-42 (in Korean).   DOI
18 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15:473-497.   DOI
19 Wu, S.Q., M.L. Lian, R. Gao, S.Y. Park and X.C. Piao. 2011. Bioreactor application on adventitious root culture of Astragalus membranaceus. In Vitro Cell Dev. Biol. Plant 47:719-724.   DOI
20 Wu, C.H., J. Tang, Z.X. Jin, M. Wang, Z.Q. Liu, T. Huang and M.L. Lian. 2018. Optimizing co-culture conditions of adventitious root of Echinacea pallia and Echinacea purpurea in air-lift bioreactor systems. Biochem. Eng. J. 132:206-216.   DOI
21 Cui, X.H., D. Chakrabarty, E.J. Lee and K.Y. Paek. 2010. Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour. Technol. 101:4708-4716.   DOI
22 Jiang, Y.J., X.C. Piao, J.S. Liu, J. Jiang, Z.X. Lian, M.J. Kim and M.L. Lian. 2015. Bioactive compound production by adventitious root culture of Oplopanax elatus in balloon-type airlift bioreactor systems and bioactivity property. Plant Cell Tiss. Org. Cult. 123:413-425.   DOI
23 Brand-Williams, W., M.E. Cuvelier and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Tech. 28:25-30.   DOI
24 Coelho, N., S. Goncalves and A. Romano. 2020. Endemic plant species conservation: biotechnological approaches. Plants 9:345.   DOI
25 George, E.F., D.J.M. Puttock and H.J. George. 1988. Plant culture media: commentary and analysis. Vol. 2. Exegetics Ltd., UK.
26 Yuan, Y., C. Brunel, M.v. Kleunen, J. Li and Z. Jin. 2019. Salinity-induced changes in the rhizosphere microbiome improve salt tolerance of Hibiscus hamabo. Plant Soil 443:525-537.   DOI
27 Seo, J.H., S.H. Lee, M.S. Do, J.S. Kim and K.H. Nam. 2021. National Biological Red Data Book Ver. 5.=Vascular Plants. National Institute of Biological Resources, Incheon, Korea. pp. 468-469 (in Korean).
28 Souza, F.V.D., F.R. Ferreira, E.H. Souza, R.L. Silva and P.A. Guerra. 2017. Conservation, propagation and new paths for pineapple genetic resources. Acta Hortic. 1239:105-112.   DOI
29 Jeong, C.S. 2007. High density culture of mountain ginseng (Panax ginseng C.A. Meyer) adventitious roots in large scale bioreactors for the production of ginseng biomass and ginsenosides. Department of Horticulture, Ph.D. Thesis, Chungbuk Natl. Univ., Korea.
30 Verpoorte, R., A. Contin and J. Memelink. 2002. Biotechnology for the production of plant secondary metabolites. Phytochem. Rev. 1:13-25.   DOI
31 Lee, S.H., J.Y. Park, K.H. Seo, J.N. Choi, Y.J. Lee, Y.H. Moon, M. Hur, W.T. Park, Y.C. Hur, J.K. Chang and S.C. Koo. 2021. Whitening and antioxidant effects of extracts from Angelica gigas Nakai thin root. Korean J. Plant Res. 34: 37-43 (in Korean).   DOI
32 Shohael, A.M., D. Chakrabarty, K.W. Yu, E.J. Hahn and K.Y. Paek. 2005. Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and production of eleutherosides. J. Biotechnol. 120:228-236.   DOI
33 Murthy, H.N., D. Dalawai, M.A. Bhat, V.S. Dandin, K.Y. Paek and S.Y. Park. 2020. Plant Cell and Tissue Differentiation and Secondary Metabolites: Biotechnological Production of Useful Phytochemicals from Adventitious Root Cultures. Springer, Switzerland. pp. 469-485.
34 Ni, L., Z. Wang, X. Liu, S. Wu, J. Hua, Y. Yin, H. Li and C. Gu. 2022. Transcriptome analysis of salt stress in Hibiscus hamabo Sieb. et Zucc based on Pacbio full-length transcriptome sequencing. Int. J. Mol. Sci. 23:138.
35 Ni, L., Z. Wang, Z. Fu, D. Liu, Y. Yin, H. Li and C. Gu. 2021. Genome-wide analysis of basic helix-loop-helix family genes and expression analysis in response to drought and salt stresses in Hibiscus hamabo Sieb. et Zucc. Int. J. Mol. Sci. 22:8748.   DOI
36 Nosov, A.M. 2012. Application of cell technologies for production of plant-derived bioactive substances of plant origin. Appl. Biochem. Microbiol. 48:609-624.   DOI
37 Rahmat, E. and Y. Kang. 2019. Adventitious root culture for secondary metabolite production in medicinal plants: a review. J. Plant Biotechnol. 46:143-157.   DOI
38 Wang, Z., L. Ni, J. Hua, L. Liu, Y. Yin, H. Li and C. Gu. 2021. Transcriptome analysis reveals regulatory framework for salt and drought tolerance in Hibiscus hamabo Siebold & Zuccarini. Forests. 12:454.   DOI
39 Han, L., X.C. Piao, J. Jiang, X.L. Jiang, C.R. Yin and M.L. Lian. 2019. A high production of flavonoids and anthraquinones via adventitious root culture of Oplopanax elatus and evaluating antioxidant activity. Plant Cell Tiss. Org. Cult. 137:173-179.   DOI
40 Park, G.G., U.J. Jang, B.Y. Kim, Y.A. Kim, B.J. Park, S.Y. Kim and H.J. Kwon. 2019. Composition comprising extracts, fractions and the isolated compounds of Hibiscus hamabo Siebold and Zucc. for skin anti-pollution. Korea Patent. 10-2018533.
41 Heim, K.E., A.R. Tagliaferro and D.J. Bobilya. 2002. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 13:572-584.   DOI
42 Kim, J.W., D.K. Kim, J.S. Park, Y.K. Lee, K.Y. Beik and S.D. Kim. 2009. Antioxidant and antimicrobial activities of shark collagens, and inhibitory actions on elastase and tyrosinase. Korean J. Food Preserv. 16:419-426 (in Korean).
43 Sakhanokho, H.F., N. Islam-Faridi, E.M. Babiker, C.D. Nelson, S.J. Stringer, and J.J.J. Adamczyk. 2020. Determination of nuclear DNA content, ploidy, and FISH location of ribosomal DNA in Hibiscus hamabo. Sci. Hort. 264:109167.   DOI