• Title/Summary/Keyword: Substrate quantity

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Food application of enzymes derived from microorganisms degrading chitin and chitosan (키틴과 키토산 분해 미생물 유래 효소의 식품에의 이용)

  • Park, Jae Kweon
    • Food Science and Industry
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    • v.53 no.1
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    • pp.43-55
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    • 2020
  • Most reports demonstrated the substrate specificity-based kinetic properties of chitin or chitosan degrading enzymes. However, there is virtually less information on the high quality and quantity production of chitin or chitosan hydrolysates having a larger than (GlcN)7 from the hydrolysis of high molecular weight chitosan using specific enzymes and their biological activity. Therefore, the production of such molecules and the discovery of such enzyme sources are very important. Fortunately, the author has established a mass production method of chitosan hydrolysates (GlcN)n, n=2-13 that have been characterized as a potent antioxidant substance, as well as antifungal and antibacterial activities against Penicillium species and highly selective pathogenic bacteria. In addition, preclinical studies using (GlcN)n, n=5-25 demonstrated that these molecules played a very important role in maintaining biometric balance. Collectively, it is implicated that the application of these mixed substances to foods with significant biological activity is very encouraging.

Dynamics of Early Fermentation of Italian Ryegrass (Lolium multiflorum Lam.)Silage

  • Shao, Tao;Ohba, N.;Shimojo, M.;Masuda, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.11
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    • pp.1606-1610
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    • 2002
  • The dynamics of fermentation were studied with Italian ryegrass ensiled in the laboratory silos. The silos were kept in the room set at 25$^{\circ}C$, and then were opened on 0.5, 1, 2, 3, 5, 7 and 14 days after ensiling, respectively. The samples were taken from three silos at each sampling time for chemical analyses. Mono-and disaccharides composition was determined for glucose, fructose and sucrose by high performance liquid chromatography. The Italian ryegrass silage succeeded to achieve lactate type fermentation; high values of lactic acid (85.83 g/kg) and lactic acid/acetic acid at the end of ensiling (14 day), low values of pH (3.74), acetic acid (5.38 g/kg), ethanol (19.20 g/kg) and $NH_3-N/Total\;N$ (75.84 g/kg), no or only small amounts of butyric acid, valeric acid and propionic acid. The fermentation dynamics showed a fast and large pH decrease caused by a fast and large production of lactic acid during the first 5 days. Mono-and disaccharides composition largely decreased within initial 0.5 day (12 h) of ensiling. Sucrose disappeared rapidly within initial 0.5 day of ensiling, and fructose and glucose contents showed an initial rise during ensiling, and then decreased gradually. These indicated that the enzymes of plant tissue were active within 2 days of ensiling, which caused the initial rise in fructose and glucose from the hydrolysis of sucrose and fructans. After 5 days of ensilage, glucose was consumed completely, suggesting that glucose was the first fermentation substrate. After 2 days of ensiling, sum amounts of lactic acid and remaining mono-and disaccharides proved to be larger than the quantity of mono-and disaccharides in the initial grass. From the facts mentioned above, it was suggested that considerable amounts of lactic acid were produced from some other substrate such as fructans than initial mono-and disaccharides.

Reaction Optimization for Enzymatic Synthesis of Astragalin (효소를 이용한 아스트라갈린 합성 반응의 최적화)

  • Lee, Seul Bi;Chung, Dae-won
    • Applied Chemistry for Engineering
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    • v.23 no.4
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    • pp.394-398
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    • 2012
  • Astragalin (AS), kaempferol monoglycoside, is classified as a polyphenol, and a minute quantity of AS is known to be present in several plants. Recently, it was reported that AS can be prepared by the partial hydrolysis of camelliaside A (CamA) and camelliaside B (CamB) in the tea seed extract (TSE) in the presence of a commercial enzyme complex such as Mash. In this paper, the effects of reaction temperature, amount of enzyme, and the substrate concentration on the reactivity were investigated. As the reaction temperature or the amount of enzyme increased, the reaction rate to produce AS increased, however, the hydrolysis of AS into KR was also enhanced. As a conclusion, the reaction, when 2 mL of Mash to 1 g of TSE was applied with a substrate concentration of 15% at $50^{\circ}C$, was found to be optimum, based on the reaction rate and the selectivity to AS.

Effect of Liquid Spawn on Flammulina velutipes Cultivation (팽이버섯 재배시 액체종균 사용 효과)

  • Ryu, Young-Hyun;Yoon, Young-Seok;Jo, Woo-Sik;Park, Sun-Do;Choi, Boo-Sull;Kim, Jong-Kuk
    • The Korean Journal of Mycology
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    • v.26 no.1 s.84
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    • pp.20-24
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    • 1998
  • The effects of liquid spawn to sawdust substrate on the growth of Flammulina velutipes were conducted for two years. The duration of optimal incubation for preculture and main culture of liquid spawn was 4 days and 6 days, respectively. When using liquid spawn, the application time for the first pinhead formation was similar with sawdust spawn, and incubation time of sawdust substrate was variable with liquid spawn and inoculum quantity. But, the overall yield of mushroom fruitbody was increased by using liquid spawn, excepting sesame hull extract medium.

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Interfacial fracture analysis of human tooth/composite resin restoration using acoustic emission (음향방출법을 이용한 치아/복합레진 수복재의 계면부 파괴해석)

  • Gu, Ja-Uk;Choi, Nak-Sam;Arakawa, Kazuo
    • Composites Research
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    • v.22 no.6
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    • pp.45-51
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    • 2009
  • The marginal integrity at the composite resin-tooth interface has been analyzed in real time through acoustic emission (AE) monitoring during the polymerization shrinkage of composite resin subjected to the light exposure. It was found that AE signals were generated by the polymerization shrinkage. Most AE hit events showed a blast type signal having the principal frequency band of 100-200kHz. Bad bonding states were indicated by many hit events in the initial curing period of 1 minute with high contraction rate. The quantity of hit events for the human molar dentin specimen was much less than that for the steel ring specimen but more than that for the PMMA ring specimen. The better the bonding state, the less the AE hit events. The AE characteristics were related with the tensile crack propagation occurring in the adhesive region between the composite resin and the ring substrate as well as the compressive behavior of the ring substrate, which could be used for a nondestructive characterization of the marginal disintegrative fracture of the dental restoration.

Selenization of CIG Precursors Using RTP Method with Se Cracker Cell

  • Kang, Young-Jin;Song, Hye-Jin;Cho, You-Suk;Yoon, Jong-Man;Jung, Yong-Deuk;Cho, Dea-Hyung;Kim, Ju-Hee;Park, Su-Jung
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.426-426
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    • 2012
  • The CIGS absorber has outstanding advantages in the absorption coefficient and conversation efficiency. The CIGS thin film solar cells have been researched for commercialization and increasing the conversion efficiency. CIG precursors were deposited on the Mo coated glass substrate by magnetron sputtering with multilayer structure, which is CuIn/CuGa/CuIn/CuGa. Then, the metallic precursors were selenized under high Se pressure by RTP method which included. Se vapor was supplied using Se cracker cell instead of toxic hydrogen selenide gas. Se beam flux was controlled by variable reservoir zone (R-zone) temperature during selenization process. Cracked Se source reacted with CIG precursors in a small quantity of Se because of small size molecules with high activation energy. The CIGS thin films were studied by FESEM, EDX, and XRD. The CIGS solar cell was also developed by layering of CdS and ZnO layers. And the conversion efficiency of the CIGS solar cell was characterization. It was reached at 6.99% without AR layer.

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Effect of Phase Separation on Anaerobic Degradation of Phenol (페놀의 혐기성분해에 대한 상분리의 영향)

  • Park, Ju-Seok;Shin, Hang-Sik;Bae, Byung-Uk
    • Journal of Korean Society of Water and Wastewater
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    • v.8 no.1
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    • pp.27-33
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    • 1994
  • With the rapid industrialization, an ever-increasing quantity and kind of new organic compounds pose environmental problems due to their toxicity and physiological effect. However, research on the biodegradation of these compounds under anaerobic condition is very limited inspite of its efficiency and economical advantage. In this research, the pH effect on the ring cleavage of phenol under anaerobic condition was investigated, and the theory of phase separation was applied to the degradation of phenol for investigating the role of acidogenic bacteria. Results, obtained from biochemical methane potential(BMP) assay for 15.5 days of incubation, showed that acidic condition was more desirable for phenol degradation than alkaline condition. By both unacclimated methanogenic granular sludge and two mixed cultures, phenol was completely removed within six weeks of incubation with a gas conversion rate of over 86% of theoretical one. However, phenol was not degraded by unacclimated acidogenic culture, and thus it is considered as a syntrophic substrate. In case of phase separated biochemical methane potential(PSBMP) assay, in which acidogenic and methanogenic culture were seeded separately and consecutively, those that had been subjected to normal acidogens for 3 to 4 weeks showed higher gas production than those seeded with sterile or frozen culture.

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Determination of New Layout in a Semiconductor Packaging Substrate Line using Simulation and AHP/DEA (시뮬레이션과 AHP/DEA를 이용한 반도체 부품 생산라인 개선안 결정)

  • Kim, Dong-Soo;Park, Chul-Soon;Moon, Dug-Hee
    • IE interfaces
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    • v.25 no.2
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    • pp.264-275
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    • 2012
  • The process of semiconductor(IC Package) manufacturing usually includes lots of complex and sequential processes. Many kinds of equipments are installed with the mixed concept of serial and parallel manufacturing system. The business environments of the semiconductor industry have been changed frequently, because new technologies are developed continuously. It is the main reason of new investment plan and layout consideration. However, it is difficult to change the layout after installation, because the major equipments are expensive and difficult to move. Furthermore, it is usually a multiple-objective problem. Thus, new investment or layout change should be carefully considered when the production environments likewise product mix and production quantity are changed. This paper introduces a simulation case study of a Korean company that produces packaging substrates(especially lead frames) and requires multi-objective decision support. $QUEST^{(R)}$ is used for simulation modelling and AHP(Analytic Hierarchy Process) and DEA(Data Envelopment Analysis) are used for weighting of qualitative performance measures and solving multiple-objective layout problem, respectively.

Damage Behaviors by Particle Impact Energy of $Al_2O_3-TiO_2$ Coated Glass Specimen ($Al_2O_3-TiO_2$ 용사코팅된 유리의 입자충격 에너지에 따른 손상거동)

  • Lee, Moon-Hwan
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.11 no.6
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    • pp.107-114
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    • 2012
  • Fracture of brittle material due to dynamic load such a particle impact has been reported by many researchers as the fracture behavior by variation of stress for a short minute. Especially, the brittle material, such a ceramic, applied to the structural component of machine, is considered as the important project. In order to evaluate the improvement of impact resistance, the particle impact test for the $Al_2O_3-TiO_2$ coated glass is practiced. And then, the damage variation according to the impact energy of steel ball was evaluated. There was a large improvement by the ceramic coating on the surface of a glass substrate. The damage volume was especially imported to evaluate damage behavior in quantity. These data were plotted on logarithmic coordinate and experimental equations were induced by data analysis based on test results. And the variation of critical energy for crack initiation was analyzed with critical impact energy when each crack occurs.

A Case Study of Comparing the Measuring Methods for Workloads of Resources in a Manufacturing Processes of Semiconductor-Parts (반도체부품 생산공정 자원의 부하 측정방법 비교분석 사례연구)

  • Kim, Dong-Soo;Moon, Dug-Hee
    • Journal of the Korea Society for Simulation
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    • v.20 no.3
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    • pp.49-58
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    • 2011
  • The workloads of facilities and laborers are important for the capacity planning in a factory. They are always referenced whenever a factory develops a new product, increases the production quantity and makes a plan of new investment. There are many measuring methods for estimating the workload effectiveness of facilities and laborers. In this paper, various measuring methods including survey, work sampling, micro-motion study, data gathering from ERP system and simulation, are analyzed for comparing the accuracy of workload. This case study is conducted in a Korean company that produces semiconductor parts like leadframe and packaging substrate.