• Title/Summary/Keyword: Stable diffusion

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Purification and Characterization of a Methanol Dehydrogenase Derived from Methylomicrobium sp. HG-1 Cultivated Using a Compulsory Circulation Diffusion System

  • Kim, Hee-Gon;Kim, Si-Wouk
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.2
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    • pp.134-139
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    • 2006
  • Methanotrophs are microorganisms that possess the unique ability to utilize methane as their sole source of carbon and energy. A novel culture system, known as the compulsory circulation diffusion system, was developed for rapid growth of methanotrophic bacteria. Methanol dehydrogenase (MDH, EC 1.1.99.8) from Methylomicrobium sp. HG-1, which belongs to the type I group of methanotrophic bacteria, can catalyze the oxidation of methanol directly into formaldehyde. This enzyme was purified 8-fold to electrophoretic homogeneity by means of a 4 step procedure and was found in the soluble fraction. The relative molecular weight of the native enzyme was estimated by gel filtration to be 120 kDa. The enzyme consisted of two identical dimers which, in turn, consisted of large and small subunits in an ${\alpha}_2{\beta}_2$ conformation. The isoelectric point was 5.4. The enzymatic activity of purified MDH was optimum at pH 9.0 and $60^{\circ}C$, and remained stable at that temperature for 20 min. MDH was able to oxidize primary alcohols from methanol to octanol and formaldehyde.

Thermal Stability Improvement of Ni-Silicide on the SOI Substrate Doped B11 for Nano-scale CMOSFET (나노급 CMOSFET을 위한 SOI기판에 도핑된 B1l을 이용한 니켈-실리사이드의 열안정성 개선)

  • Jung, Soon-Yen;Oh, Soon-Young;Lee, Won-Jae;Zhang, Ying-Ying;Zhong, Zhun;Li, Shi-Guang;Kim, Yeong-Cheol;Lee, Ga-Won;Wang, Jin-Suk;Lee, Hi-Deok
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.11
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    • pp.1000-1004
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    • 2006
  • In this paper, thermal stability of Ni-silicide formed on the SOI substrate with $B_{11}$ has been characterized. The sheet resistance of Ni-silicide on un-doped SOI and $B_{11}$ implanted bulk substrate was increased after the post-silicidation annealing at $700^{\circ}C$ for 30 min. However, in case of $B_{11}$ implanted SOI substrate, the sheet resistance showed stable characteristics after the post-silicidation annealing up to $700^{\circ}C$ for 30 min. The main reason of the excellent property of $B_{11}$ sample is believed to be the retardation of Ni diffusion by the boron and bottom oxide layer of SOI. Therefore, retardation of Ni diffusion is highly desirable lot high performance Ni silicide technology.

Proliferation of Smart Agriculture through Advanced ICT Technology (ICT 기술 고도화를 통한 스마트농업 확산)

  • Kim, Joo-Man;Chung, Wonho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.4
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    • pp.117-122
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    • 2018
  • This paper suggests smart agricultural diffusion strategy through advanced ICT technology. Today, the world is threatened by environmental pollution and traditional warming due to global warming, and the decrease in agricultural workers due to low fertility and aging is expected to bring social problems to future food resources. The convergence of ICT technology and agriculture is not a labor-intensive primary industry, but a new paradigm that includes cultivation, manufacturing and services. It is urgent to spread smart farm technology that can supply stable food with low labor force. In this paper, we review the current state of smart farm technology, analyze the impediments to diffusion, and present the direction of smart agricultural development in the future by upgrading ICT technology.

Dependence on Dopant of Ni-silicide for Nano CMOS Device (Nano CMOS소자를 위한 Ni-silicide의 Dopant 의존성 분석)

  • 배미숙;지희환;이헌진;오순영;윤장근;황빈봉;왕진석;이희덕
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.40 no.11
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    • pp.1-8
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    • 2003
  • In this paper, the dependence of silicide properties such as sheet resistance and cross-sectional profile on the dopants for source/drain and gate has been characterized. There was little difference of sheet resistance among the dopants such as As, P, BF$_2$ and B$_{11}$ just a(ter formation of NiSi using RTP (Rapid Thermal Process). However, the silicide properties showed strong dependence on the dopants when thermal treatment was applied after silicidation. BF$_2$ implanted silicon showed the most stable property, while As implanted one showed the worst. The main reason of the excellent property of BF$_2$ sample is believed to be tile retardation of hi diffusion by the flourine. Therefore, retardation of Ni diffusion is highly desirable for high performance Ni-silicide technology.y.

Improvement of ESD Protection Performance of High Voltage Operating EDNMOS Device with Double Polarity Source (DPS) Structure (DPS(Double Polarity Source) 구조를 갖는 고전압 동작용 EDNMOS 소자의 정전기 보호 성능 개선)

  • Seo, Yong-Jin;Yang, Jun-Won
    • Journal of Satellite, Information and Communications
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    • v.9 no.2
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    • pp.12-17
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    • 2014
  • In this paper, modified EDNMOS device with DPS (double polarity source) structure are suggested to realize stable and robust ESD (electrostatic discharge) protection performance of high voltage operating microchip. This DPS structure inserts the P+ diffusion layer on N+ source side, which in intended to block lateral extension of the electron rich region from N+ source side. Based on our simulation results, the inserted P+ diffusion layer effectively prevents the formation of deep electron channeling induced by high electron injection. As a result, our proposed DPS_EDNMOS devices could overcome the double snapback effect of conventional Std_EDNMOS device.

Corrosion Properties of Dissimilar Friction Stir Welded 6061 Aluminum and HT590 Steel

  • Seo, Bosung;Song, Kuk Hyun;Park, Kwangsuk
    • Metals and materials international
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    • v.24 no.6
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    • pp.1232-1240
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    • 2018
  • Corrosion properties of dissimilar friction stir welded 6061 aluminum and HT590 steel were investigated to understand effects of galvanic corrosion. As cathode when coupled, HT590 was cathodically protected. However, the passivation of AA6061 made the aluminum alloy cathode temporarily, which leaded to corrosion of HT590. From the EIS analysis showing Warburg diffusion plot in Nyquist plots, it can be inferred that the stable passivation layer was formed on AA6061. However, the weld as well as HT590 did not show Warburg diffusion plot in Nyquist plots, suggesting that there was no barrier for corrosion or even if it exists, the barrier had no function for preventing and/or retarding charge transport through the passivation layer. The open circuit potential measurements showed that the potential of the weld was similar to that of HT590, which lied in the pitting region for AA6061, making the aluminum alloy part of the weld keep corrosion state. That resulted in the cracked oxide film on AA6061 of the weld, which could not play a role of corrosion barrier.

DEVELOPMENT OF POLYETHOXYLATED RETINAMIDE AS AN ANTl-AGINC AGENT

  • Song, Young-Sook;Chung, Bong-Yul;Chang, Min-Youl;Park, Mun-Eok;Lee, Sung-Jun;Cho, Wan-Goo;Kang, Seh-Hoon
    • Proceedings of the SCSK Conference
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    • 1999.10a
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    • pp.145-154
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    • 1999
  • A novel retinol derivative, polyethoxylated retinamide (Medimin A) was synthesized, as an anti-aging agent. Collagen synthesis, skin permeation, stability, and toxicity of Medimin A were evaluated and compared with those of retinol and retinyl palmitate. In vitro collagen synthesis was evaluated by quantitative assay of [$^3H$]-proline incorporation into collagenase sensitive protein in fibroblast cultures. For in vitro skin permeation experiments, Franz diffusion cells (effective diffusion area: $1, 766{\;}\textrm{cm}^2$) and the excised skin of female hairless mouse aged 8 weeks were used The stabilities of retlnoids were evaluated at two different temperature ($25{\;}^{\circ}C$ and $40{\;}^{\circ}C$) and under UV in solubilized state and in OW emulsion. To estimate the safety, acute oral toxicity, acute dermal toxicity, primary skin irritation, acute eye irritation and human patch test were performed The effect of Medimin A on collagen synthesis was similar to that of retinol. The skin permeability of Medimin A was higher than those of retinol and retinyl palmitate. The Medimin A was more stable than retinol and retinyl palmitate. Medimin A was nontoxic in various toxicological tests. These results suggest that Medimin A would be a good anti-aging agent for enhancing bioavailability and stability.

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A Comparative study on the structure of plan in folk houses of Korea and Japan (韓 . 日 民家의 平面構造 比較硏究)

  • ;Chang, Bo-Woong
    • Journal of the Korean Geographical Society
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    • v.29 no.1
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    • pp.3-15
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    • 1994
  • The purpose of this study is to investigate the origin of traditional folkhouses of Japan in connection with those of Korea. Japanese folkhouses have received much influence from the Korean Peninsular, mainland China, northern Asia and Southeast Asia. The writer believes that the main stream of folkhouse culture origineted in the Korean Peninsular and flowed into the Japanese Islands. There ara many striking similarities in the folkhouses of Korea and Japan. Firstly, double-row room houses are distributed in the northeastern part of the Korean Penininsular and all parts of the Japanese Islands, and they are classified into the five-room type and four-room type. These types are very similar in both nations in the aspects of and floorplan and distributing patterns. Because floorplan may be used as an indicator of culture regions and cultural diffusion both, nations are believed to be closely related in the interchange of cultures in ancient times. Secondly, stables are allocated to the main house, and they are called "magu" in common "Magu" means horse stable, but there are no horses in it. In ancient times, however, many horses were kept in "magu" in both countries. Thirdly, there are much similarities in the function and structure of the folkhouses of both countries. The characteristics of folkhouses of Japan could be explained as the result of cultural diffusion from the Korean Peninsular.on from the Korean Peninsular.

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A Numerical Study for the Air Flow on Complex Terrain (복잡지형의 공기흐름에 대한 수치해석 연구)

  • Park, Mi Sun;Jeong, Hae Sun;Jeong, Hyo Joon;Hwang, Won Tae;Kim, Eun Han;Han, Moon Hee;Kim, Hey Suk
    • Journal of Radiation Protection and Research
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    • v.39 no.2
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    • pp.70-80
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    • 2014
  • The interpretation on the diffusion of radiation contaminants in air is usually to apply a Gaussian plume equation that obtains normal distributions in stable air flow conditions to draw a conservative conclusion. In this study, a numerical study using computational fluid dynamics methods was performed to interpret the air flow pattern and the diffusion of the radiation contaminants at the Wolseong nuclear power plants, and a more detailed solution can be obtained than the Gaussian plume equation, which is difficult to use to simulate complex terrains. The results show that a significant fluctuation of air flow in the terrain appears in the case of a northwester and southeaster because of the mountain located in the northwest and the sea located in the south-east. The northwesterly air flow shows the most unstable flow in the vertical direction when it passes over the terrain of mountain. The stable southeasterly air flow enters into the nuclear power plant from the sea, but it becomes unstable rapidly because of the interference by the building and the terrain. On the other hand, in the case of a northeaster and southwester, a small interruption of air flow is caused by the terrain and wake behind the buildings of nuclear power plants.

Mixing Process of Double Diffusive Salt Wedge (이중확산의 영향을 받는 염수침입의 혼합과정 연구)

  • Hwang, Jin-Hwan
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.11 no.2
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    • pp.92-97
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    • 2008
  • Salt wedge into the river from the sea or fresh water flume (fresh wedge) in the ocean from the sea has density current characteristics. However, when temperature and salinity simultaneously determine the density of wedges, one of salinity and temperature can distributed in the reversed profiles against gravity, even though the density profile is stable. In this case, the double diffusive process is critical in determining mixing rate. The present work studies relative contribution of shear driven mechanical mixing component and double diffusive layering process, when warm salty denser water is introduced into the cold fresh lighter water column. Laboratory experiment releases warm salty denser water into cold fresh lighter water controlling discharge amount to achieve the steady state of density current. When longitudinal density rate becomes 15, the released amount ratio of salt and heat changes sharply and in the releasing point, vigorous mixing occurs with increase of discharged amount due to double diffusion. Double diffusion distabilizes gravitational stability and enhances the mixing rate up to $6{\times}10$ times at the lower density ratio comparing to the higher density ratio.

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