• Journal of Plant Biotechnology
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• 제49권1호
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• pp.15-29
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• 2022

Cyclophilins (CYPs) are highly conserved ubiquitous proteins belong to the peptidyl prolyl cis/trans isomerase (PPIase) superfamily. These proteins are present in a wide range of organisms; they contain a highly conserved peptidyl-prolyl cis/trans isomerase domain. A comprehensive database survey identified a total of 35 genes localized in all cellular compartments of Solanum lycopersicum L., but largely in the cytosol. Sequence alignment and conserved motif analyses of the SlCYP proteins revealed a highly conserved CLD motif. Evolutionary analysis predicted the clustering of a large number of gene pairs with high sequence similarity. Expression analysis using the RNA-Seq data showed that the majority of the SlCYP genes were highly expressed in mature leaves and blooming flowers, compared with their expression in other organs. This study provides a basis for the functional characterization of individual CYP genes in the future to elucidate their role(s) in protein refolding and long-distance signaling in tomatoes and in plant biology, in general.

• BMB Reports
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• 제44권10호
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• pp.692-697
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• 2011

To investigate the pathways of oxidoreductases in plants, 2 key components in thioredox systems i.e. thioredoxin h (Trx h) and NADPH-dependent thioredoxin reductase (NTR) genes were first isolated from tomatoes (Solanum lycopersicum). Subsequently, the coding sequences of Trx h and NTR were inserted into pET expression vectors, and overexpressed in Escherichia coli. In the UV-Visible spectra of the purified proteins, tomato Trx h was shown to have a characteristic 'shoulder' at ~290 nm, while the NTR protein had the 3 typical peaks unique to flavoenzymes. The activities of both proteins were demonstrated by following insulin reduction, as well as DTNB reduction. Moreover, both NADPH and NADH could serve as substrates in the NTR reduction system, but the catalytic efficiency of NTR with NADPH was 2500-fold higher than with NADH. Additionally, our results reveal that the tomato Trx system might be involved in oxidative stress, but not in cold damage.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.155.2-156
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• 2003

The adducts of lipid peroxidation and related aldehydic end are mediators of chronic poisoning and affect the development of chronic liver damage leading to fibrosis and cirrhosis. Substances delayed or suppresed lipid peroxidation could have an antioxidant and protective effect in liver disease. In this study, it was attempted to find out above mentioned effect of Solanum lycopersicum investigated in CCl4 induce liver fibrosis model. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.86.2-87
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• 2003

Introduction: Liver fibrosis is defined unbalance of collagen metabolism, especially a stimulation of collagen synthesis and inhibition of collagen degradation, and antifibrotic effect is delaying or inhibition of new collagen synthesis and deposition in liver tissue. In this study, we investigated the antifibrotic and antioxidative effect of Solanum lycopersicum (SL) in liver fibrosis induced rats. Methods: : Rats were randomly divided in three groups (normal, CCl$_4$ and CCl$_4$-SL group) and were received 0.6 ml mixture of CCl$_4$ and olived oil (1:1 v/v) 3 times/week for 4 weeks except of the normal group. (omitted)

• 식물병연구
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• 제28권2호
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• pp.108-111
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• 2022

In December 2020, stem and leaf rot symptoms in small-fruited tomato (Solanum lycopersicum) plants were observed in a farmer's vinyl greenhouse located in Pyeongtaek, Gyeonggi Province, Korea. The incidence of diseased plants in the vinyl greenhouse was 2-6%. Seven single-spore isolates of Phoma sp. were obtained from the diseased stems and leaves. All the isolates were identified as Boeremia linicola based on the cultural, morphological and molecular characteristics. Two isolates of B. linicola were tested for pathogenicity on stems and leaves of small-fruited tomato and large-fruited tomato using artificial inoculation. All the tested isolates caused stem and leaf rot symptoms in the inoculated plants. The symptoms were similar to those observed in plants from the vinyl greenhouse investigated. This is the first report of B. linicola causing stem and leaf rot in tomato.

• 생물환경조절학회지
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• 제19권1호
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• pp.12-18
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• 2010

토마토를 대상으로 암면과 코이어를 사용한 자루재배에서 일사량제어와 배액전극제어법의 급액제어 능력을 실험한 결과, 배지 종류에 관계없이 일사량제어법에 비해 배액전극제어법에서 배지함수량과 배액량이 안정 적이었다. 총수확량과 상품과량은 배액전극제어법에서 많았으며, 동일한 급액제어 처리 안에서는 배지간 차이는 없었다. 당도는 급액제어 방법의 차이보다는 배지의 종류에 영향을 많이 받았다. 배액전극제어법은 펄라이트뿐만 아니라 암면과 코이어배지에서도 범용적으로 일사량제어법에 비해 유리한 것으로 나타났다.

• The Plant Pathology Journal
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• 제34권5호
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• pp.435-444
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• 2018

Receptor-like proteins (RLPs) are involved in plant development and disease resistance. Only some of the RLPs in tomato (Solanum lycopersicum L.) have been functionally characterized though 176 genes encoding RLPs, which have been identified in the tomato genome. To further understand the role of RLPs in tomato, we performed genome-guided classification and transcriptome analysis of these genes. Phylogenic comparisons revealed that the tomato RLP members could be divided into eight subgroups and that the genes evolved independently compared to similar genes in Arabidopsis. Based on location and physical clustering analyses, we conclude that tomato RLPs likely expanded primarily through tandem duplication events. According to tissue specific RNA-seq data, 71 RLPs were expressed in at least one of the following tissues: root, leaf, bud, flower, or fruit. Several genes had expression patterns that were tissue specific. In addition, tomato RLP expression profiles after infection with different pathogens showed distinguish gene regulations according to disease induction and resistance response as well as infection by bacteria and virus. Notably, Some RLPs were highly and/or unique expressed in susceptible tomato to pathogen, suggesting that the RLP could be involved in disease response, possibly as a host-susceptibility factor. Our study could provide an important clues for further investigations into the function of tomato RLPs involved in developmental and response to pathogens.

• BMB Reports
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• 제44권12호
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• pp.805-810
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• 2011

Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and $K_m$ and $K_{cat}$ values for MSRA2 were observed as 8.5, $25^{\circ}C$, $352{\pm}25\;{\mu}M$, and $0.066{\pm}0.009\;S^{-1}$, respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage.

• Journal of Plant Biotechnology
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• 제47권1호
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• pp.78-89
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• 2020

기능성 성분이 향상된 토마토 품종 개발을 위해서는 성분 정량분석법과 토마토 유전자원 간 대사성분 변이에 대한 정보의 확보가 필요하다. 본 연구에서는 토마토 유전자원23개 계통과 12개 상용 F₁ 품종을 이용하여 수용성 비타민 7종(vitamin C, B1, B2, B3, B5, B6, B9)과 폴리페놀계 성분 5종(quercetin, rutin, kaempferol, myricetin, and naringenin chalcone)에 대한 함량을 비교 분석 하였다. HPLC와 LC-MS 분석 결과, 수용성 비타민과 폴리페놀계의 주요 성분으로 vitamin C와 naringenin chalcone이 각각 검출되었으며 품종 간 높은 수준의 함량 변이가 존재함을 알 수 있었다. 반면에 vitamin B1, quercetin 과 kaempferol은 전 품종에 있어 함량이 가장 낮았다. 대사성분 함량과 토마토 과실특성 간 상관관계에 있어 서 과크기(과중)와 높은 유의성이 관찰되었는데 대부분의 성분에 있어 방울토마토 품종이 완숙용 토마토 품종보다 높은 함량을 보였다. 하지만 naringenin chalcone을 제외하고 대사성분과 과색 간의 상관관계는 뚜렷하게 나타나지 않았다. 본 결과는 토마토 육종과정에 활용될 수 있는 효율적인 대사성분 정량분석법을 제시할 뿐만 아니라 기능성 성분 고함량 육종소재 선발에 중요한 정보를 제공한다.

• 한국식품과학회지
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• 제50권3호
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• pp.339-343
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• 2018

과열증기와 열풍(과열증기/열풍건조) 또는 원적외선(과열증기/원적외선건조)으로 복합건조한 방울토마토의 이화학특성을 상응하는 열풍건조 토마토와 비교하여, 복합건조가 건조토마토의 품질특성에 미치는 영향을 조사하였다. 방울토마토의 수분함량 85.10%는 열풍건조, 과열증기/열풍 또는 원적외선의 복합건조에 따라 15.98, 22.94 및 23.08%로 각각 감소하였으며, 열풍건조와 달리 복합건조 토마토는 중간수분식품의 수분함량에 해당하는 반건조상태를 나타내었다. 건조토마토의 수축도와 표면색상은 건조 방법에 관계없이 유사하였으나, 열풍건조 토마토는 복합건조에 비해 단단한 텍스처와 낮은 풍미를 나타내었다. 방울토마토의 아스코브산 함량(260.1 mg/100 g)은 열풍건조에서 77.6 mg/100 g으로 최대 감소를 보인 반면, 복합건조의 경우에는 과열증기/열풍건조 120.8 mg/100 g, 과열증기/원적외선건조 185.2 mg/100 g으로 낮은 손실을 나타내었다. 리코펜 함량은 열풍건조, 과열증기/열풍 또는 원적외선의 복합건조에서 각각 5.55, 5.81 및 8.53 mg/100 g으로서, 대조구 대비 77, 76 및 64%의 높은 손실률을 나타내었는데, 이는 건조공정 이외에 시료의 탈피와 동결건조 과정에서 리코펜이 유실, 손상되었기 때문으로 추정된다. 그러나 건조방법에 따른 리코펜의 손실 양상을 고려할 때, 리코펜 손실은 과열증기/원적외선의 복합건조에서 가장 낮은 것으로 판단된다. 한편 수분흡수력은 모든 수침시간에서 복합건조시료가 열풍건조에 비해 현저하게 높았으며, 특히 과열증기/원적외선건조의 경우 가장 빠른 수화복원력을 나타내었다. 이상의 결과에 따라 방울토마토의 건조는 외관, 유용성분의 손실률과 수화복원력의 관점에서 과열증기처리 후 원적외선으로 건조하는 복합건조가 열풍건조에 비해 효율적인 것으로 판단된다.