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http://dx.doi.org/10.5483/BMBRep.2011.44.12.805

Characterization of a novel methionine sulfoxide reductase A from tomato (Solanum lycopersicum), and its protecting role in Escherichia coli  

Dai, Changbo (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
Singh, Naresh Kumar (Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University)
Park, Myung-Ho (Department of Mechanical Engineering, Kangwon National University)
Publication Information
BMB Reports / v.44, no.12, 2011 , pp. 805-810 More about this Journal
Abstract
Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and $K_m$ and $K_{cat}$ values for MSRA2 were observed as 8.5, $25^{\circ}C$, $352{\pm}25\;{\mu}M$, and $0.066{\pm}0.009\;S^{-1}$, respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage.
Keywords
Kinetics parameters; Micro-Tom; MSRA; Oxidative damage; Reverse phase HPLC;
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Times Cited By Web Of Science : 1  (Related Records In Web of Science)
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