• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.62-68
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• 2011

Calmodulin (CaM), a $Ca^{2+}$ binding protein in eukaryotes, mediates cellular $Ca^{2+}$ signals in response to a variety of biotic and abiotic external stimuli. The $Ca^{2+}$-bound CaM transduces signals by modulating the activities of numerous CaM-binding proteins. As a CaM binding protein, AtCBP63 ($\b{A}$rabidopsis thaliana $\b{C}$aM-binding protein $\underline{63}$ kD) has been known to be positively involved in plant defense signaling pathway. To investigate the pathogen resistance function of AtCBP63 in potato, we constructed transgenic potato (Solanum tuberosum L.) plants constitutively overexpressing AtCBP63 under the control of cauliflower mosaic virus (CaMV) 35S promoter. The overexpression of the AtCBP63 in potato plants resulted in the high level induction of pathogenesis-related (PR) genes such as PR-2, PR-3 and PR-5. In addition, the AtCBP63 transgenic potato showed significantly enhanced resistance against a pathogen causing bacterial soft rot, Erwinia carotovora ssp. Carotovora (ECC). These results suggest that a CaM binding protein from Arabidopsis, AtCBP63, plays a positive role in pathogen resistance in potato.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.49-53
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• 2011

The present study was performed to select suitable potato (Solanum tuberosum L.) varieties for double cropping in seed production using aeroponic system, the nutrient solution was sprayed for 20 seconds in every 3 minutes, and potatoes were planted into styrofoam at a distance of 25 cm between plants. Transplantation was completed in September 9 and potatoes were harvested in November 12. The 10 trial cultivars, 'Atlantic', 'Dejima', 'Haryong', 'Hongyoung', 'Jayoung', 'Jopung', 'Jowon', 'Namsuh', 'Seohong', 'Superior' were examined for growth and yield. The shoot growth of all cultivars at the early stage was better than 'Superior', however, no difference was observed at the late stage of plant growth. In addition, 'Superior' showed a excellent root growth during the early plant growth stage, however, increased root growth was only found in 'Jayoung', 'Seohong', during the late plant growth stage. The stolon weight was greater in the order of 'Seohong'>'Superior'>'Jayoung'>'Hongyoung'. Particularly, the number of tuber in 'Jowon' was 22.4/plant that was over twice than 'Superior'(11.3/plant). As results of the current study, therefore, we concluded that four cultivars 'Seohong', 'Jayoung', 'Hongyoung', 'Jowon'were the most suitable cultivars for basic seed production using aeroponic system in Autumn potato.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.42-48
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• 2011

Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast transformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.300-304
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• 2012

In order to research changes of asparagine content according to stage after harvest to storage, it was analysed asparagine content of potato tuber and potato sprout. The results of the asparagine content in potato cultivars after harvest ranked in cv. Superior, cv. Gahwang, cv. Atlantic and cv. Goun in descending order. The content of asparagine in potato was approximately 1300 mg/100g (D.W.) The changes the asparagine content according to storage condition and the difference of asparagine between tuber and sprout can be summarized as follows. In the asparagine content of potato cultivars, Little variation in the content was observed while potato tubers were stored at $4^{\circ}C$. However, after 3 months of storage at $10^{\circ}C$, the content increased by 9.7%, indicating that the content increases as the storage temperature and period increases. The content of asparagine also increased during sprouting of potato tubers. In the sprout, the proximal region contained 21% higher content of asparagines than the distal region, which suggests that the sprout develops with the increasing of the content of asparagines.

• Korean Journal of Weed Science
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• v.31 no.3
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• pp.240-249
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• 2011

The potential of juglone a plant naphthoquinone as a natural herbicide on new target, 7-keto-8-amino pelargonic acid synthetase (KAPAS) in the early step of biotin biosynthesis pathway, was performed in vitro and in vivo. Juglone effectively inhibited KAPAS activities in vitro and the $IC_{50}$ was $9.5{\mu}M$. Foliar application of juglone showed very good herbicidal activity to the eight-tested weed species. Among them, Solanum nigrum was completely controlled at a concentration of $250{\mu}g\;mL^{-1}$ with main symptoms of desiccation or burndown. Digitaria sanguinalis and Aeschynomene indica were also sensitive to juglone treatment. All eight weed species were controlled by 90~100% at a concentration of $500{\mu}g\;mL^{-1}$. However, soil application of juglone to Digitaria sanguinalis did not show any herbicidal symptoms. Cellular leakage from cucumber leaf squares treated with juglone increased depending on the concentrations increased from 6.25 to $100{\mu}M$ after 24 hours incubation with or without light. However, chlorophyll loss in cucumber leaf squares was negligible. Biotin supplements significantly rescued the inhibition of germination rate of Arabidopsis thaliana seeds previously inhibited by the juglone. Our results suggest that the juglone is a possible environmental friendly herbicide candidate with a new target KAPAS inhibiting activity.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.117-123
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• 2017

Invasive alien species (IAS) can affect agricultural ecosystem negatively and critically. The geographical distributions of nine IAS plants in Korean orchards and uplands were investigated. Ambrosia artemisiifolia distributed widely in Korean peninsula and Ambrosia trifida distributed intensively in Gyeonggi-do. Aster pilosus distributed in Gyeonggi-Gangwon region and Lactuca scariola distributed linear region from the north-western to south-eastern part of Korea. Hypochaeris radicata distributed mainly in Jeju-do and Solanum carolinense distributed in eastern Gyeonggi-do. Rumex acetosella and Sicyos angulatus sporadically occurred in Korean croplands and Eupatorium rugosum was found in one region only. The distribution of IAS plants in croplands was very similar to that in non-croplands suggesting that ecological position of arable lands is not different from that of non-arable lands. Therefore, IAS plants in the croplands should be managed for not only prevention of yield reduction, but also protection or maintenance of original ecosystem. Development of management methods apposite to current Korean status are required to control IAS plants in croplands.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.771-780
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• 2014

In this study, we aimed to understand the effect of sorbitol and salicylic acid (SA) on quality and functional food contents of tomato. Sole or combined application of sorbitol and SA in different concentrations (0.5 mM and 1.0 mM) greatly improved the characteristics of tomato, such as red color tone, soluble solid content, hardness and weight as well as increasing the functional food contents such as of lycopene, anthocyanins and vitamins in comparison with the control. The cyanidin content increased approximately 52-61% and delphinidin contents also increased 20-24% and 39-41% in tomato plants treated with SA alone and sorbitol with SA, respectively. Furthermore, the sole or combined application of sorbitol and SA also increased the vitamin $B_1$ and C contents. In particular, vitamin C content was increased 1.2-fold by treatment with sorbitol alone and was increased 2-fold by SA treatment compared to the control. In the sorbitol with SA treatments, vitamin C content was approximately 4-fold increased relative to control plants.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.2
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• pp.166-174
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• 2018

Potato(Solanum tuberosum L.) is one of the major food crop in the world following rice, wheat, and maize. It is thus important to project yield predict of potato under climate change conditions for assessment of food security. A crop growth modelling is widely used to simulate crop growth condition and total yield of various crops under a given climate condition. The decision support system for agrotechnology transfer (DSSAT) cropping system model, which was developed by U.S. which package integrating several models of 27 different crops, have been used to project crop yield for the impact assessment of climate change on crop production. In this study, we simulated potato yield using RCP 8.5 climate change scenario data, as inputs to the DSSAT model in five regions of Korea. The genetic coefficients of potato cultivar for 'superior', which is one of the most widely cultivated potato variety in Korea were determined. The GenCalc program, which is a submodule of the DSSAT package, was used to determine the genetic coefficients for the superior cultivar. The values of genetic coefficients were validated using results of 39 experiments performed over seven years in five regions. As a case study, the potato yield was projected that total yields of potato across five regions would increase by 26% in 2050s but decrease by 17% in 2090s, compared with 2010s. These results suggested that the needs for cultivation and irrigation technologies would be considerably large for planning and implementation of climate change adaptation for potato production in Korea.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.13-18
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• 2003

From 1996 to 1999, potato-growing areas in Korea were surveyed for identification and distribution of potato scab pathogens. Potato scab was widely distributed in the mass cultivation areas, especially in Jriu island, southern areas of Chonnam and Gyounggi provinces, and the alpine area of Gangwon province. Jeju island was the most affected area by this disease. A total of 55 Streptomyces strains were isolated from potato scab lesions, among which 40 strains were pathogenic on progeny tubers. Among the pathogenic strain, 21 strains were identified as previously described S. scabies, 7 Strains as S. turgidiscabies, and 5 Strains as S. acidiscabies, while 7 strains were observed as having distinct phenotypic properties. These strains were classified into six distinct clusters based on phenotypic characteristics and selected representative strains for each cluster. S. scabies (S33) had grey spores in a spiral chain. Mean-while, S. turgidiscabies (S27) had grey spores, S. acidiscabies (S71) had white spores, S. luridiscabiei (S63) had yellow-white spores, S. puniciscabiei (S77) had purple-red spores, and S. niveiscabiei (S78) had thin and compact white spores, all in a rectiflexuous chain. Pathogenicity was determined by the production of thaxtomin A and homologs of necl and ORFtnp genes. In TLC, representative strains S27, S71, S63, S77, and S78 produced a yellow band that co-migrated with the authentic thaxtomin A. However, thaxtomin A was not detected in chloroform extracts from oatmeal broth culture and Slice tuber tissue of S. luridiscabiei (S63) and S. puniciscabiei (S77) by HPLC analysis. In addition, no homologs of necl and ORFtnp genes in S. acidiscabies (S71), S. luridiscabiei (S63), S. puniciscabiei (S77), and S. niveiscabiei (S78) were detected by PCR and Southern hybridization analysis.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.3
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• pp.164-169
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• 2008

This experiment was carried out to find the growth response and changes of nitrate and soluble sugar content in tomato leaves with salt stress. Tomato (Solanum lycopericum) seedlings were grown under different electrical conductivity (EC) levels adjusted with $CaCl_2$ as 1, 2, and $6dS\;m^{-1}$. The growth response and contents of nitrate and soluble sugar in tomato plants were examined at 7 and 14 days after salt treatment. Leaf area and dry weight ratio of shoot to root of tomato plants were decreased as EC level increased. Photosynthetic rate of leaves was reduced under high EC level due to the stomatal closure and the reduction of transpiration rate. The soluble sugar and starch content were lower in the tomato leaves grown under high EC level. Total nitrogen and nitrate contents were decreased in high EC level, whereas the ammonium content was increased. High-salt stress induced the accumulation of salt crystal in mesophyll cells of tomato leaf.