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http://dx.doi.org/10.5010/JPB.2011.38.1.062

AtCBP63, a Arabidopsis Calmodulin-binding Protein 63, Enhances Disease Resistance Against Soft Rot Disease in Potato  

Chun, Hyun-Jin (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology)
Park, Hyeong-Cheol (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center)
Goo, Young-Min (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology)
Kim, Tae-Won (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology)
Cho, Kwang-Soo (Highland Agriculture center, National Institute of Crop Science, RDA)
Cho, Hyeon-Seol (Department of Physical Therapy, Gwangyang Health College)
Yun, Dae-Jin (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center)
Chung, Woo-Sik (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center)
Lee, Shin-Woo (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Plant Biotechnology / v.38, no.1, 2011 , pp. 62-68 More about this Journal
Abstract
Calmodulin (CaM), a $Ca^{2+}$ binding protein in eukaryotes, mediates cellular $Ca^{2+}$ signals in response to a variety of biotic and abiotic external stimuli. The $Ca^{2+}$-bound CaM transduces signals by modulating the activities of numerous CaM-binding proteins. As a CaM binding protein, AtCBP63 ($\b{A}$rabidopsis thaliana $\b{C}$aM-binding protein $\underline{63}$ kD) has been known to be positively involved in plant defense signaling pathway. To investigate the pathogen resistance function of AtCBP63 in potato, we constructed transgenic potato (Solanum tuberosum L.) plants constitutively overexpressing AtCBP63 under the control of cauliflower mosaic virus (CaMV) 35S promoter. The overexpression of the AtCBP63 in potato plants resulted in the high level induction of pathogenesis-related (PR) genes such as PR-2, PR-3 and PR-5. In addition, the AtCBP63 transgenic potato showed significantly enhanced resistance against a pathogen causing bacterial soft rot, Erwinia carotovora ssp. Carotovora (ECC). These results suggest that a CaM binding protein from Arabidopsis, AtCBP63, plays a positive role in pathogen resistance in potato.
Keywords
Calmodulin; Disease resistance; Pathogenesisrelated gene; Potato; Soft rot;
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1 Sjefke JHM, Florack DEA, Hoogendoorn C and Stiekema WJ (1995) Erwinia soft rot resistance of potato cultivars transformed with a gene construct coding for antimicrobial peptide cecropin B is not altered. Amer J Potato Research 72:437-445   DOI
2 Snedden WA and Fromm H (2001) Calmodulin as a versatile calcium signal transducer in plants. New Phytol 151:35-66   DOI
3 Takezawa D, Liu ZH, An G and Poovaiah BW (1995) Calmodulin gene family in potato: developmental and touch-induced expression of the mRNA encoding a novel isoform. Plant Mol Biol 27:693-703   DOI
4 Wang L, Tsuda K, Sato M, Cohen JD, Katagiri F, Glazebrook J. (2009) Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae. PLoS Pathog 5 (2):e1000301   DOI
5 Yamakawa H, Mitsuhara I, Ito N, Seo S, Kamada H, Ohashi Y (2001) Transcriptionally and post-transcriptionally regulated response of 13 calmodulin genes to tobacco mosaic virusinduced cell death and wounding in tobacco plant. Eur J Biochem 268:3916-3929   DOI
6 Yang T, Segal G, Abbo S, Feldman M and Fromm H (1996) Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects. Mol Gen Genet 252:684-694   DOI
7 Yi JY, Seo HW, Yang MS, Robb EJ, Nazar RN and Lee SW (2004) Plant defense gene promoter enhances the reliability of shiva-1 gene-induced resistance to soft rot disease in potato. Planta 220:165-171   DOI
8 Yoo JH, Park CY, Kim JC, Heo WD, Cheong MS, Park HC, Kim MC, Moon BC, Choi MS, Kang YH, Lee JH, Kim HS, Lee SM, Yoon HW, Lim CO, Yun DJ, Lee SY, Chung WS and Cho MJ (2005) Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in Arabidopsis. J Biol Chem 280:3697-3706   DOI
9 Lee SH, Johnson JD, Walsh MP, Van Lierop JE, Sutherland C, Xu A, Snedden WA, Kosk-kosicka D, Fromm H, Narayanan N and Cho MJ (2000) Different regulation of $Ca^{2+}$/calmodulindependent enzymes by plant calmodulin isoforms and free $Ca^{2+}$ concentration. Biochem J 350:299-306   DOI
10 Lee SH, Kim JC, Lee MS, Heo WD, Seo HY, Yoon HW, Hong JC, Lee SY, Bahk JD, Hwang I, Cho MJ (1995) Identification of a novel divergent calmodulin isoform from soybean which has differential ability to activate calmodulin-dependent enzymes. J Biol Chem 270:21806-21812   DOI
11 Park HC, Kang YH, Chun HJ, Koo JC, Cheong YH, Kim CY, Kim MC, Chung WS, Kim JC, Yoo JH, Koo YD, Koo SC, Lim CO, Lee SY and Cho MJ (2002) Characterization of a stamenspecific cDNA encoding a novel plant defensin in Chinese cabbage. Plant Mol Biol 50:59-69
12 Ling V, Perera I and Zielinski RE (1991) Primary structures of Arabidopsis calmodulin isoforms deduced from the sequences of cDNA clones. Plant Physiol 96:1196-1202   DOI
13 Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473-497   DOI
14 Park CY, Heo WD, Yoo JH, Lee JH, Kim MC, Chun HJ, Moon BC, Kim IH, Park HC, Choi MS, Ok HM, Cheong MS, Lee SM, Kim HS, Lee KH, Lim CO, Chung WS and Cho MJ (2004) Pathogenesis-related gene expression by specific calmodulin isoforms is dependent on NIM1, a key regulator of systemic acquired resistance. Mol. Cells 18:207-213   과학기술학회마을
15 Rhoads AR, Friedberg F (1997) Sequence motifs for calmodulin recognition. FASEB J 11:331-341   DOI
16 Sacks WR, Ferreira P, Hahlbrock K, Jabs T, Nürnberger T, Renelt A and Scheel D (1993) Elicitor recognition and intracellular signal transduction in plant defense. In: Advances in Molecular Genetics of Plant-Microbe Interactions. (Nester, E., ed.) Kluwer Academic publishers Dordrecht, Boston, London Vol 2:485-495
17 Agrios GN (1988) Plant pathology, 3rd edn. Academic Press, New York, pp 17-26
18 Chin D and Means AR (2000) calmodulin: a prototypical calcium sensor. Trends Cell Biol 10:322-328   DOI
19 Cho MJ, Vaghy PL, Kondo R, Lee SH, David JP, Rehl R, Heo WD and Johnson JD (1998) Reciprocal regulation of mammalian nitric oxide synthase and calcineurin by plant calmodulin isoforms. Biochemistry 37:15593-15597   DOI
20 Dixon RA, Harrison MJ and Lamb CJ (1994) Early events in the activation of plant defense responses. Annu Rev Phytopathol 32:479-501   DOI
21 Dolmetsch RE, Lewis RS, Goodnow CC and Healy JI (1997) Differential activation of transcription factors induced by $Ca^{2+}$ response amplitude and duration. Nature 24:855-858
22 Heo WD, Lee SH, Kim MC, Kim JC, Chung WS, Chun HJ, Lee KJ, Park CY, Park HC, Choi JY and Cho MJ (1999) Involvement of specific calmodulin isoforms in salicylic acid-independent activation of plant disease resistance responses. Proc Natl Acad Sci USA 96:766-771   DOI
23 Hoeflich KP and Ikura M (2002) Calmodulin in action: diversity in target recognition and activation mechanisms Cell 108: 739-742   DOI
24 Kim MC, Chung WS,Yun D-J and Cho MJ (2009a) Calcium and calmodulin-mediated regulation of gene expression in plants. Mol Plant 2:13-21   DOI
25 Kim SH, Kang YH, Han HJ, Bae DW, Kim MC, Lim CO and Chung WS (2009b) Identification of another calmodulinbinding domain at the C-terminal region of AtCBP63. J Plant Biotechnol 36:53-58   DOI
26 Kondo R, Tikunova SB, Cho MJ and Johnson JD (1999) A point mutation in a plant calmodulin is responsible for its inhibition of nitric-oxide synthase. J Biol Chem 274:36213-36218   DOI