Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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AtCBP63, a Arabidopsis Calmodulin-binding Protein 63, Enhances Disease Resistance Against Soft Rot Disease in Potato

애기장대 칼모듈린 결합 단백질 AtCBP63을 발현시킨 형질전환 감자의 무름병 저항성 증가

  • Chun, Hyun-Jin (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology) ;
  • Park, Hyeong-Cheol (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center) ;
  • Goo, Young-Min (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology) ;
  • Kim, Tae-Won (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology) ;
  • Cho, Kwang-Soo (Highland Agriculture center, National Institute of Crop Science, RDA) ;
  • Cho, Hyeon-Seol (Department of Physical Therapy, Gwangyang Health College) ;
  • Yun, Dae-Jin (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center) ;
  • Chung, Woo-Sik (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center) ;
  • Lee, Shin-Woo (Divison of Agronomy & Medicinal Resources, Gyeongnam National University of Science and Technology)
  • 전현진 (경남과학기술대학교 농학.한약자원학부) ;
  • 박형철 (경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작 연구소) ;
  • 구영민 (경남과학기술대학교 농학.한약자원학부) ;
  • 김태원 (경남과학기술대학교 농학.한약자원학부) ;
  • 조광수 (국립식량과학원, 고령지농업연구센터) ;
  • 조현설 (광양보건대학 물리치료과) ;
  • 윤대진 (경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작연구소) ;
  • 정우식 (경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작연구소) ;
  • 이신우 (경남과학기술대학교 농학.한약자원학부)
  • Received : 2011.03.02
  • Accepted : 2011.03.12
  • Published : 2011.03.31
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Abstract

Calmodulin (CaM), a $Ca^{2+}$ binding protein in eukaryotes, mediates cellular $Ca^{2+}$ signals in response to a variety of biotic and abiotic external stimuli. The $Ca^{2+}$-bound CaM transduces signals by modulating the activities of numerous CaM-binding proteins. As a CaM binding protein, AtCBP63 ($\b{A}$rabidopsis thaliana $\b{C}$aM-binding protein $\underline{63}$ kD) has been known to be positively involved in plant defense signaling pathway. To investigate the pathogen resistance function of AtCBP63 in potato, we constructed transgenic potato (Solanum tuberosum L.) plants constitutively overexpressing AtCBP63 under the control of cauliflower mosaic virus (CaMV) 35S promoter. The overexpression of the AtCBP63 in potato plants resulted in the high level induction of pathogenesis-related (PR) genes such as PR-2, PR-3 and PR-5. In addition, the AtCBP63 transgenic potato showed significantly enhanced resistance against a pathogen causing bacterial soft rot, Erwinia carotovora ssp. Carotovora (ECC). These results suggest that a CaM binding protein from Arabidopsis, AtCBP63, plays a positive role in pathogen resistance in potato.

원예작물의 생육을 저하시키는 각종 병충해로 인한 과도한 농약과 화학비료의 사용은 환경오염뿐만 아니라 작물의 생산량에도 큰 영향을 미치고 있다. 식물생명공학기술을 이용하여 농약이나 화학비료 사용량을 획기적으로 줄일 수 있는 식물체의 개발, 즉 형질전환을 이용한 분자 육종기술은 병충해 내성 농작물을 개발하여 과도한 화학비료의 사용에 따르는 여러가지 문제점들을 극복할 수 있는 대안으로 대두되고 있다. 본 연구에서는 모델식물인 애기장대에서 분리한 식물생체방어 신호전달에 관련된 AtCBP63 유전자를 감자에 과발현시켰고, 이러한 형질 전환 감자에서 병저항성에 관여하는 유전자인 PR-2, PR-3, PR-5 유전자들의 발현이 증가되어 지속적으로 식물 방어 기작이 활성화되어 있음을 확인하였다. 또한, 감자에서 무름병 (soft rot disease)을 일으켜 막대한 피해를 유발하는 병원성 세균인 Erwinia carotovora subsp. Carotovora (Ecc)를 이용하여 AtCBP63 유전자를 과발현한 감자에 감염시켰을 때, 병 저항성이 증가한다는 사실을 검증하였다. 앞으로, 다양한 곰팡이 균에 대응하여 AtCBP63 유전자를 과발현한 감자에 저항성을 검증하고자 한다.

Keywords

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