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Studies on Biological Activity of Woad Extractives (XV) - Antimicrobial and antioxidative activities of extracts from diverse families - (수목 추출물의 생리활성에 관한 연구(XV) - 과별(科別)에 따른 항균 및 항산화 활성 -)

  • Lee, Sung-Suk;Lee, Hak-Ju;Choi, Don-Ha
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.4
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    • pp.8-17
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    • 2004
  • Antifungal, antibacterial, and antioxidative activities of ethanol extracts from 65 families 263 species were investigated to select tree species for the utilization of natural fungicide or preservative resources. The antifungal activities of extracts from wood, leaf and bark were measured as hyphal growth inhibition rate using four plant pathogenic and five wood rotting fungi. High inhibitory effect on the fungi growth was found in five species of Pinaceae (Pinus koraiensis, P. rigida, P. densiflora, P. banksiana. Cedrus deodara), three species of Cupressaceae (Juniperus rigida, J. chinensis, Chamaecyparis obtusa) and three species of Leguminosae (Albizzia julibrisssin, Sophora japonica, Maackia amurensis), respectively. Antibacterial activities of ethanol extracts were determined by means of disc-agar plate diffusion method using three gram-positive and five gram-negative bacteria. The ethanol extracts, which showed prominent effect on the suppression of bacteria growth, were six species of Betulaceae (Carpinus tschonoskii, C. coreana, C. laxiflora, Alnus hirsuta, A. firma, Betula schmidtii), five species of Fagaceae (Castanopsis cuspidata var. sieboldii, Quercus serrata, Q. mongolica, Q aliena, C crenata), four species of Euphorbiaceae (Aleurites fordii, Sapium sebiferum, S japonicum Mallotus japonicus) and three species of Elaeagnaceae (Elaeagnus umbellata, Elaeagnus glanbra, Elaeagnus macrophylla). According to these results, the extracts from Zelkova serrata, Pinus densiflora, Maackia amurensis, Chamaecyparis obtusa and Juniperus chinensis could be available for natural fungicide or food preservatives, because ethanol extracts from these species indicated excellent antifungal and antibacterial activities. In order to test antioxidative activities of ethanol extracts, free radical scavenging method was adopted with 1,1-diphenyl-2-picrylhydrohydrazyl (DPPH). Free radical scavenging activity was proved very high in the extracts of eight species of Rosaceae (Eriobotrya japonica, Prunus takesimensis, P yedoensis, P padus, P armeniaca var. ansu, Chaenomeles sinensis, Stephanandra incisa, Rosa multiflora) and five species of Ericaceae (Rhododenron mucronulatum, R. scblippenbacbii, R. yedoense var. poukhanense, Vaccinium bracteatum, V oldbami), resvectively. It turned out from this study that only six species among 48 species of Rosaceae showed less than 80% free radical scavenging activity. As a consequences, it could be deduced that the components effective on antioxidative activity commonly exist in Rosaceae plant family.

Distribution of Seabird in Coastal Wetland of Korea in 2015-2019 (2015-2019년 한국 연안습지에 서식하는 바닷새 분포 현황)

  • KIM, YONG-HEE;LEE, YONG-WOO;PARK, CHI-YOUNG;HAN, SEUNG-WOO;LEE, SI-WAN;KIM, YOUNG-NAM;CHO, SOO-YEON;HWANG, INSEO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.27 no.3
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    • pp.158-172
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    • 2022
  • The distribution of seabirds was investigated in 24-34 major coastal wetlands in Korea from 2015 to 2019. During the study period, seven orders, 16 families, and 111 species were observed, and the largest number of species (51-89 species) was recorded in 2018. 92,821-265,783 individuals per year were observed with the largest number in 2018. The largest number of species (55-72 species) was observed in the southern sites of Jeollado, and the largest number of individuals (annual average of 62,542 individuals) was found in Chungcheong regions. The largest number of seabird species (68 species) was observed at the Suncheon Bay site in 2016, and the largest number of individuals (95,399 individuals) was observed at Biin and Jangan sites in 2018. In terms of the habitat type of seabirds, at the family level, shorebirds appeared most frequently with an average of 38%, followed by waterfowl, wading birds, seabirds, and raptors. With regard to legally protected species, eight protected marine species, 19 endangered wildlife species (Level I: six species, Level II: 13 species), and twelve natural monument species were observed. Overall, there was a significant difference in the number of species and individuals of seabirds in the Korean coastal wetlands during the research period. This suggests the importance of conducting systematic and continuous seabird surveys and developing a conservation policy considering habitat characteristics.

Distribution of Flora and Naturalized Plants in Gwangpo Bay, Sacheon (사천시 광포만의 식물상 및 외래식물 분포)

  • Lee, Min-Sook;Kim, Jung-Soo;Park, Sam-Bong;Cho, Gab-Ja;An, Jong-Bin;Song, Jin-Heon;Kwag, Chil-Sig;Kim, Bong-Gyu;Choo, Gab-Chul
    • Journal of Korean Society of Forest Science
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    • v.110 no.3
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    • pp.369-392
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    • 2021
  • This study aimed to provide basic data for efficient flora management of vascular plants, halophytes, and naturalized plants in the Gwangpo Bay area in Sacheon city, Gyeongsangnam-do. The flora found in this area incorporated 520 taxa, including 93 families, 299 genera, 466 species, 3 subspecies, 41 varieties, and 10 forma, and accounted for approximately 10.6% of total vascular plants (4,884 species) in Korea. According to the survey, 76 species of Gramineae showed the highest frequency (14.6%), followed by 67 species of Compositae (12.8%), 34 species of Leguminosae (6.5%), 27 species of Cyperaceae (5.2%), and 22 species of Rosaceae (4.2%). Rare plant species designated by the Korea Forest Service were categorized into six taxa, including three taxa in the LC grade, one taxon in the CR grade, one taxon in the VU grade, and one taxon in the EN grade. Korea endemic plants belonged to nine taxa consisting of seven families, seven genera, and nine species. The special floristic plants included 44 taxa, including one taxon (Prunus yedoensis) in the V grade, four taxa in the IV grade, ten taxa in the III grade, six taxa in the II grade, and 23 taxa in the I grade. Naturalized plants included 62 taxa consisting of 15 families, 47 genera, 59 species and 3 varieties. Halophytes consisted of 27 taxa, including 13 families, 24 genera, 24 species, 2 varieties, 1 forma, which consisted of Atriplex gmelinii, Suaeda glauca, Suaeda maritima, and Lathyrus japonicus. Invasive alien species in the Gwangpo Bay area included seven taxa. Since Ambrosia artemisiifolia, Sicyos angulatus, andPaspalum distichum var. indutummay have harmful effects on native plants, these plants must be artificially controlled.

The Ecological Management and Characteristics of Bird Communities at the World Cup Park in Seoul (서울시 월드컵공원 야생조류 군집 특성 및 생태적 관리방안)

  • Han Bong-Ho;Kim Jeong-Ho;Son Byong-Dof;Lee Kyong-Jae
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.5 s.112
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    • pp.43-56
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    • 2005
  • This study was conducted to examine the inhabitation of wild birds at the World Cup Park and to offer the Park ecological management data. Land use md actual vegetation were examined to analyze the inhabitation of wild birds. Characteristics of bird communities were analyzed by dominance, density, diversity index and guild concept. You compare inhabitation before and after the Park was built. We classified the land use and actual vegetation into twenty-six types. Robinia pseudoacacia dominated the study area. Pyeonghwa Park and Nanji-cheon Park were simple-layer structures composed of alien woody species. The observed birds after the World Cup Park included 33 families 77 species, and 9,751 individuals. Among Wild Life types, the shrub and bush type was dominant. There were 26 species of resident birds, 20 species of summer visitors, 18 species of winter visitors, and 12 species of passage migrants. The following numbers of species and individuals were observed: in Hanul park, 38 species, 3,151 individuals; in Noul park, 45 species, 2,061 individuals; in Nanji-cheon park, 42 species, 2,742 individuals; in Pyeonghwa park, 29 species, 875 individuals; and in Maebongsan(Mt.) area, 35 species, 922 individuals. Species diversities for each area were as follows: Noul park, 2.613; Hanul park, 2,301; Nanji-cheon, 2.228; and Pyeonghwa park, 2,019; and each season: summer, 2.652; spring, 2.650: winter, 2.561; and autumn 2.176. The diversity of species increased from 1.135 in 1994 to 2.324 in 2001. We recommended that the park be differented into different ecological areas in order to encourage the appearance of wild birds at the World Cup Park. The management area was divided into three districts(conservation area, preservation and restoration area, use area). The conservation area was established to the west of a waste landfill and in the downstream area of NanJi-cheon, the preservation and restoration area was established in the midstream area of Nanji-cheon, and the use area was established in the buffer zone of Pyeonghwa Park and the Nanji pond greenspace.

Development and Application of PCR-based Markers for the Discrimination of Bang-Poong and Related Species (방풍류의 감별을 위한 분자마커의 탐색과 활용)

  • Hong, Seong-Mi;Lee, Mi-Young;Koh, Jae-Chul;Ko, Byoung-Soeb
    • Journal of Plant Biotechnology
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    • v.31 no.1
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    • pp.1-6
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    • 2004
  • Bang-Poong and related species are an important herbal medicine. However, it is difficult to determine the commercial dry material through anatomical and chemotaxonomical characteristics. Here, we used a PCR-based technique for an accurate discrimination of Bang-Poong and related species. With the RAPD primers, 215 RAPDSs(random amplified polymorphic DNAs) were obtained, and 98% of them showed polymorphic patterns. RAPDs from the four primers were appropriate for the discrimination of S. divaricata $(T_{URCZ{\cdot}})\;S_{CHISKIN}$, those from the six primers for P. japonicum $T_{HUNBERG}$, those from the four primers for P. terebinthaceum $F_{ISHER}$, and those from the six primers for G. littoralis Fr. $S_{CHMIDT}$. The specific bands from the primer 425 were obtained and used to develop SCAR (sequence characterized amplified region) markers, based on the sequence information of the RAPD markers. The SCAR primers generated a 215 bp fragment specific to Peucedanum terebinthaceum $F_{ISHER}$, and a 177 bp and a 300 bp fragment specific to G. littoralis Fr. $S_{CHMIDT}$. As a result, the three SCAR markers were able to discriminate from two Bang-Poong related species.

Phylogeny of the subfamily Salmoninae distributed in Korea based upon nucleotide sequences of mitochondrial ribosomal RNA genes (미토콘드리아 ribosomal RNA 유전자 염기서열분석에 의한 한국산 연어아과 어류의 유전적 계통도)

  • LEE Heui-Jung;PARK Jung-Youn;LEE Jeong-Ho;MIN Kwang-Sik;JEON Im Gi;YOO Mi-Ae;LEE Won-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.2
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    • pp.103-109
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    • 2000
  • Complete senuences of the mitochondrial rRNA Benes were determined among six salmonines in Korean Waters (Brachpmystax lenok, Onoorhpchus keta, O. masou mason, O. mason ishikawae, O. mykiss, and albino mutant of O. mykiss). The purposes of this study were to provide the basic information on levels of mtDNA polymorphism among these species for genetic characterization; discuss phylogentic relationships among three Oncorhynchus sepecies; demonstrate the utility of rRNA gene sequence data as a genetic marker for disringuishinf among Korean salmonines. PCR/direct sequencing data indicated the following consistent results; 1) 12S rRNA genes was 945 bases long in Oncorhynchus species, and 946 bases in B. lenot including one insertion. 2) Of sequence variation in mitochondrial rRNA regions, transitional substitutions were superior to transversion. 3) The significant differences were not shown in the intraspecific variation values in these gene regions. The percentage sequence divergence values were ranged from $0.066 to 0.212{\%}$. 4) The interspecific divergences were greater than the intraspecific variation. Nevertheless, ribosomal RMh genes were more conserved among species than the other mitochondrial genes, and they showed potentiality as an intergenic marker for systematics. In addition, phylogenetic trees, constructed from this data, supported that cherry salmon was closer to chum salmon than to rainbow trout, and that lenok was most distantly related species in six salmonid species.

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Classification of One-Crewmen Coastal Fishing Boats by the Fish Species caught with A Multivariate Analysis (어획어종의 다변량분석에 의한 1인승 연안어선의 분류)

  • Jeong, Dong-Gun;Choi, Chan-Moon
    • Journal of Fisheries and Marine Sciences Education
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    • v.9 no.2
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    • pp.222-235
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    • 1997
  • On the basis of the seven species of fish caught by fishing boats with one crewmen belonging to the Iwawada Fisheries Cooperative of Chiba Prefecture, the fishing boats were classified by species with high market values, and the results obtained were reclassified by discriminant function. As a result, the fishing boats were classified into six groups. These six groups are : G1 featuring the main catches of yellowtails ; G2 flounders ; G3 skipjack tunas, G4 squids ; G5 demersal fish, and G6 other miscellaneous species. Furthermore, principal component analysis were carried out on fish catches of the seven species in terms of the value obtained from a catch from the scores of the first, second, third and fourth principal components. The results of analysis show that fishing boats with one crewman can be broadly classified into three groups ; i.e., Groups G1/G2, Groups G3/G4/G5 and Group G6.

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The study for grading of the mudflat by birds (조류에 의한 갯벌의 등급화 연구)

  • Lee, Ki Sup;Kim, Mi Ran;Lee, Si Wan;Lee, Han Soo
    • Journal of Wetlands Research
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    • v.6 no.1
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    • pp.105-115
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    • 2004
  • This study was carried out to grade and evaluate the intertidal mudflat by birds. The mudflat was graded by two methods such as the scoring and ranking. First, for the scoring of the mudflat, five factors were estimated; population size, the number of protected species, the individuals of protected birds, the number of species(or subspecies) over 1% level of the survival population, and 1% level of the domestic population. After scoring of these five factors, the grade was scored by total. Second, for the ranking, six factors were estimated; population size, the density of birds, the index of species diversity, species richness, species evenness, and population stability. After ranking of these six factors, the grade was relatively ranked. Five grade by scoring was the important mudflat for the value of international preservation, and the most mudflats of the estuary gained V grade. The higher grades by ranking were the mudflats of Nakdonggang and Mankyunggang river estuaries. And the inner mudflats were more valuable than the outer mudflats in Mankyunggang & Dongjingang river estuaries. The grading of the mudflat by birds can be used for estimating and appointing of the important mudflats, and suggested the objective of criteria for the effective preservation and management.

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A Study on the prescriptions of 『Sanbeon-bang(刪繁方)』 (『산번방(刪繁方)』의 의방(醫方)에 관한 연구)

  • Kim, Do-Hoon;Jeong, Chang-Hyun
    • The Journal of Korean Medical History
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    • v.17 no.2
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    • pp.111-127
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    • 2004
  • This paper is mainly on the rsearch of the prescriptions of "Sanbeon-bang(刪 繁方)". For the research of prescription, investigated "Sanbeon-bang" from the side of symptoms of a disease, number of drugstuffs, table of contents, drugstuffs and acupuncture and moxibustion. With these investigation, made out a few tables, and with these tables made an attempt to understand the whole prescriptions of "Sanbeon-bang". "Sanbeon-bang" is of rich contents in internal medicine. Among internal medicine, related to Oro-Yukgeuk-Chilsang(五勞-六極-七傷) was most abundant. So we can guess "Sanbeon-bang" was a medical book specialized in exhaustion syndromes. It also deals with the Samcho-syndromes, as investigate from medical comments, has the perfect system of prescription in relative. From the investigation of the combination of drugstuffs, ascertained that, the number of simple prescription composed of one drugstuff was 38, combinational prescription composed of two drugstuffs was 9, minor prescription composed of three to five drugstuffs was 47, midum prescription composed of six to ten drugstuffs was 95, major prescription composed of eleven to twenty drugstuffs was 45, and mixed prescription composed over twenty drugstuffs was 1. Mentionable unique prescriptions in internal use were the forms of keeping in mouth. In external use they were fumigants, suppositories, powdered medicines, spraying the granular medication into the nasal cavity and eyedrops. And were abundant in soft extract(ointment) and plaster. In addition, there were the recordings of 18 types of cellulitis, types of incurable cellulitis and curing an illness by a charm. By the way, when comparing "Sanbeon-bang" with "Cheongeum-bang", all of which was quoted a lot in "Oedaebiyo-bang", "Cheongeum-bang" rather took medical comments of "Sanbeon-bang" than prescription. Although there were some prescriptons in "Sanbeon-bang" at the same category, "Cheongeum-bang" took another prescriptions which are more complexed than those of "Sanbeon-bang". In the same way, when comparing "Cheongeum-bang" with "Sonjinin-Cheongeum-bang(孫眞人千金方)", which didn't go through the correction of GyojeongUiseoguk of Bug-Song goverment, "Cheongeum-bang" often didn't take the prescriptions of "Sonjinin-Cheongeum-bang". Hence we can guess, "Cheongeumyo-bang" may have added a lot of prescriptions when undergoing the correction of of GyojeongUiseoguk. The total number of species of drugstuffs in "Sanbeon-bang" from the investigation was 284. The plant drugs were 208 species, the animal were 31 species, the minerals were 19 species and the other were 26 species. The prescriptions related to acupuncture and moxibustion in "Sanbeon-bang" were only moxibustional prescriptions. Which appeared one time per exhaustion and steaming of bone syndrome, Oro-Yukgeuk-Chilsang and Samcho syndrome. Appeared six times in muscle syndromes. But I cannot imagine the original form of acupuncture and moxibustion in "Sanbeon-bang" for deficiency of data.

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Characteristics of Fish Community on Six Lakes Located in Gyeonggi (경기 6개호수의 어류군집 특성)

  • Kim, Jai-Ku;Jang, Young-Su;Lee, Kwang-Yeol;Ryu, Hyeung-Rial;Jeong, Ju-Yong;Kim, Bom-Chul;Choi, Jae-Seok
    • Korean Journal of Ecology and Environment
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    • v.39 no.2 s.116
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    • pp.178-186
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    • 2006
  • Community structures of fish in six small eutrophied lakes, located in Gyeonggi province, Korea were investigated from September 2003 to August 2004. Total number of fish species was 33 species of 13 families in which five Korean endemic species such as Rhodeus uyekii, Squalidus gracilis majimae, Abbottina springeri, Iksookimia koreensis, and Odontobutis interrupta were found. Dominant species in the lakes was Hemiculter leucisculus. Both Zacco platypus and Pseudorasbora parva were numberous as subdominant species. In particlilar, Micropercops swinhonis known as partly distributed in the Jeonlabukdo was first recorded in Lake Wangsong and Heungbu which are located in the Gyeonggi province. In lake Myukwoo, Wangsong, and Heungbu, the proportion of Pelagic and omnivorous fish were high, reflecting that fish habitat is poor. A CPUE based fish production was the lowest in Lake Geumgwang of which lake is relatively favorable, whereas it was higher in eutriphied lakes suchs as Dukwoo, Myukwoo and Heungbu lakes.