Browse > Article
http://dx.doi.org/10.5010/JPB.2004.31.1.001

Development and Application of PCR-based Markers for the Discrimination of Bang-Poong and Related Species  

Hong, Seong-Mi (Department of Inspection Enterprise, Korea Institute of Oriental Medicine)
Lee, Mi-Young (Department of Inspection Enterprise, Korea Institute of Oriental Medicine)
Koh, Jae-Chul (Department of Life Resources, Catholic University of Daegu)
Ko, Byoung-Soeb (Department of Inspection Enterprise, Korea Institute of Oriental Medicine)
Publication Information
Journal of Plant Biotechnology / v.31, no.1, 2004 , pp. 1-6 More about this Journal
Abstract
Bang-Poong and related species are an important herbal medicine. However, it is difficult to determine the commercial dry material through anatomical and chemotaxonomical characteristics. Here, we used a PCR-based technique for an accurate discrimination of Bang-Poong and related species. With the RAPD primers, 215 RAPDSs(random amplified polymorphic DNAs) were obtained, and 98% of them showed polymorphic patterns. RAPDs from the four primers were appropriate for the discrimination of S. divaricata $(T_{URCZ{\cdot}})\;S_{CHISKIN}$, those from the six primers for P. japonicum $T_{HUNBERG}$, those from the four primers for P. terebinthaceum $F_{ISHER}$, and those from the six primers for G. littoralis Fr. $S_{CHMIDT}$. The specific bands from the primer 425 were obtained and used to develop SCAR (sequence characterized amplified region) markers, based on the sequence information of the RAPD markers. The SCAR primers generated a 215 bp fragment specific to Peucedanum terebinthaceum $F_{ISHER}$, and a 177 bp and a 300 bp fragment specific to G. littoralis Fr. $S_{CHMIDT}$. As a result, the three SCAR markers were able to discriminate from two Bang-Poong related species.
Keywords
Bang-Poong and related species; discrimination; RAPD; SCAR marker;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ministry of Health and Welfare, Japan (1996) The Japanese pharmacopeia 13th ed. D-980
2 Naqvi N, Chattoo BB (1996) Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice. Genome 39: 26-30   DOI   ScienceOn
3 Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism ampliRed by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531 -6535   DOI   ScienceOn
4 Yuan Z, Tezuka Y, Fan W, Kadota S, Li X (2002) Constituents of the underground parts of Glehnia IittoraIis. Chem Pharm Bull 50: 73- 77   DOI   ScienceOn
5 Arnedo AM, Gil-Ortega R, Luis AM, Hormaza J (2002) Development of RAPD and SCAR markers linked to the Pvr4 locus for resistance to PVY in pepper (Capsicum annum L.). Theor Appl Genet 105: 1067-1074   DOI   ScienceOn
6 Bulat SA, L$\ddot u$beck M, Alekhina IA, Jensen DF, Knudser IM, L$\ddot u$beck PS (2000) Identification of a universally primed-PCR-derived sequence-characterized amplified region marker for an antagonistic strain of Clonostachys rosea and development of a strain-specific PCR detection assay. Appl Environ Microbiol 66: 4758-4763   DOI   ScienceOn
7 Choi HY, Lee Sl, Suh YB (1997) PCR-mediated RFLP to identify 'Bangpoong', a crude drug. Kor J Pharmacogn 28:1-8
8 Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantaties of fresh leaf tissue. Phytochem Bull 19: 11-15
9 Ardiel GS, Grewal TS, Deberdt P, Rossnagel BG, Scoles GJ (2002) Inheritance of resistance to covered smut in barely and development of a tightly linked SCAR marker. Theor Appl Genet 104:457-464   DOI   ScienceOn
10 Hemandez P, Dorado Q, Ramirez MC, Laurie DA, Snape JW, Martin A (2003) Development of cost-effective Hordeum chilense DNA markers: molecular aids for marker-assisted cereal breeding. Hereditas 138: 54-8   DOI   ScienceOn
11 Korea Food and Drug Administration (2002a) The Korean pharmnacoepia 8th edition. pp 1309; pp 1560
12 Korea Food and Drug Administration (2002b) The Korean herbal pharmacoepia. pp 247
13 Lee WC (1996) Coloured standard illustrations of Korean plants. Academy Co Ltd. pp 257-260
14 Megi MS, Devic M, Delseny M, Lakshmikumaran M (2000) Identification of AFLP fragments linked to seed coat colour in Brassica Juncea and conversion to a SCAR marker for rapid selection. TheorAppl Genet 101: 146-152   DOI   ScienceOn
15 Okuyama E, Hasegawa T, Matsushita T, Fujimoto H, Ishibashi M, Yamazaki M (2001) Analgesic components of saposhnikovia root (Saposhnikouia diuaricata). Chem Phann Bull 49:154-60   DOI   ScienceOn
16 Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985-993
17 Wang J, Ha WY, Ngan FN, But PP, Shaw PC (2001) Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterants. Planta Med 67: 781-783   DOI   ScienceOn
18 Pharmacopoeia Commission of the Ministry of Public Health, P. R. China (1995 edition) A coloured atlas of the Chinese materia medica specified in pharmacopoeia of the People's Republic of China. Guangdong Science and Technology Press. pp 190-123
19 Randig O, Bongiovanni M, Cameiro RM, Castagnone-Sereno P (2002) Genetic diversity of root-knot nematodes from Brazil and development of SCAR markers specific for the coffee-damaging species. Genome 45: 862-870   DOI   ScienceOn
20 Ryu DS, Yook CS (1972) Coumarin components in the root of Peucedanum terebinthaceum. Kor J Pharmacogn 3: 215-216