• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.1026-1033
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• 2022

This study presents a novel DNA part characterization technique that increases throughput by combinatorial DNA part assembly, solid plate-based quantitative fluorescence assay for phenotyping, and barcode tagging-based long-read sequencing for genotyping. We confirmed that the fluorescence intensities of colonies on plates were comparable to fluorescence at the single-cell level from a high-end, flow-cytometry device and developed a high-throughput image analysis pipeline. The barcode tagging-based long-read sequencing technique enabled rapid identification of all DNA parts and their combinations with a single sequencing experiment. Using our techniques, forty-four DNA parts (21 promoters and 23 RBSs) were successfully characterized in 72 h without any automated equipment. We anticipate that this high-throughput and easy-to-use part characterization technique will contribute to increasing part diversity and be useful for building genetic circuits and metabolic pathways in synthetic biology.

• Structural Engineering and Mechanics
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• 제81권6호
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• pp.677-689
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• 2022

Generally, the goal of seismic retrofit design of an existing structure using energy dissipation devices is to determine the optimum design parameters of a retrofit device to satisfy a specified limit state with minimum cost. However, the presence of multiple parameters to be optimized and the computational complexity of performing non-linear analysis make it difficult to find the optimal design parameters in the realistic 3D structure. In this study, genetic algorithm-based optimal seismic retrofit methods for determining the required number, yield strength, and location of steel slit dampers are proposed to retrofit an asymmetric soft first-story structure. These methods use a multi-objective and single-objective evolutionary algorithms, each of which varies in computational complexity and incorporates nonlinear time-history analysis to determine seismic performance. Pareto-optimal solutions of the multi-objective optimization are found using a non-dominated sorting genetic algorithm (NSGA-II). It is demonstrated that the developed multi-objective optimization methods can determine the optimum number, yield strength, and location of dampers that satisfy the given limit state of a three-dimensional asymmetric soft first-story structure. It is also shown that the single-objective distribution method based on minimizing plan-wise stiffness eccentricity turns out to produce similar number of dampers in optimum locations without time consuming nonlinear dynamic analysis.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권2호
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• pp.559-575
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• 2023

3D human pose estimation is widely applied in various fields, including action recognition, sports analysis, and human-computer interaction. 3D human pose estimation has achieved significant progress with the introduction of convolutional neural network (CNN). Recently, several researches have proposed the use of multiview approaches to avoid occlusions in single-view approaches. However, as the number of cameras increases, a 3D pose estimation system relying on a CNN may lack in computational resources. In addition, when a single host system uses multiple cameras, the data transition speed becomes inadequate owing to bandwidth limitations. To address this problem, we propose a distributed real-time 3D pose estimation framework based on asynchronous multiple cameras. The proposed framework comprises a central server and multiple edge devices. Each multiple-edge device estimates a 2D human pose from its view and sendsit to the central server. Subsequently, the central server synchronizes the received 2D human pose data based on the timestamps. Finally, the central server reconstructs a 3D human pose using geometrical triangulation. We demonstrate that the proposed framework increases the percentage of detected joints and successfully estimates 3D human poses in real-time.

• 한국산업융합학회 논문집
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• 제26권1호
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• pp.133-141
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• 2023

Accurate measurement of seafloor topography plays a crucial role in developing marine industries such as maritime safety, resource exploration, environmental protection, and coastal management. The seafloor topography is constructed using side scan sonar (SSS) and single beam echosounder (SBES) or multibeam echosounder (MBES), which transmit and receive ultrasound waves through a device attached to a marine survey vessel. However, the use of a sonar system is affected by noise pollution areas, and the single beam has a limited scope of application. At the same time, the multibeam is mainly applicable for depth observation. For these reasons, it is difficult to determine the boundaries and areas of seafloor topography. Therefore, this study proposes a method to improve the backscatter data of multibeam echosounder, which has a relationship with the seafloor quality, by using digital image processing to classify the shape of the underwater surface.

• 재활치료과학
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• 제7권2호
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• pp.17-27
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• 2018

목적 : 본 연구는 만성 뇌졸중 환자를 대상으로 완전 몰입형 가상현실 기기를 이용한 작업치료 중재가 환자의 상지기능 향상에 미치는 효과를 알아보고자 하였다. 연구방법 : 본 연구의 연구 대상은 좌측 편마비를 가지고 있는 만성 뇌졸중 환자 1명이며, 개별사례 실험연구 중 ABA 설계를 사용하였다. 기초선 기간 4회, 중재 기간 12회, 기초선 회귀기간 4회로 총 20회의 실험을 휴지기간을 포함하여 총 10주간 진행하였다. 독립변수인 완전 몰입형 가상현실 기기를 이용한 작업치료 중재는 매 회기 30분씩 시행되었으며 종속변수 중 Fugl-Meyer Assessment(FMA) 평가는 각 기간 마다 1회씩 측정하였다. Box and Block Test(BBT) 평가와 Wolf Motor Function Test(WMFT) 평가는 매 회기 마다 시행하여 그 측정 결과를 꺾은선 그래프를 통해 제시하였다. 결과 : 완전 몰입형 가상현실 기기를 이용한 작업치료 중재 후 대상자의 환측 상지기능이 향상되었다. 기초선 회귀 기간에는 중재를 제거한 뒤 치료유지 효과를 확인하였지만 큰 변화는 나타나지 않았다. 결론 : 본 연구의 결과, 완전 몰입형 가상현실 기기를 이용한 작업치료 중재가 만성 뇌졸중 환자의 상지기능에 효과적인 중재라는 것을 알 수 있었다. 다만 치료유지 효과는 크지 않았기 때문에 보다 사용이 간편한 가정용 중재 프로그램 등의 개발이 필요하다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.174-174
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• 2010

Gallium Nitride(GaN) attracts great attention due to their wide band gap energy (3.4eV), high thermal stability to the solid state lighting devices like LED, Laser diode, UV photo detector, spintronic devices, solar cells, sensors etc. Recently, researchers are interested in synthesis of polycrystalline and amorphous GaN which has also attracted towards optoelectronic device applications significantly. One of the alternatives to deposit GaN at low temperature is to use Single Source Molecular Percursor (SSP) which provides preformed Ga-N bonding. Moreover, our group succeeds in hybridization of SSP synthesized GaN with Single wall carbon nanotube which could be applicable in field emitting devices, hybrid LEDs and sensors. In this work, the GaN thin films were deposited on c-axis oriented sapphire substrate by MBE (Molecular Beam Epitaxy) using novel single source precursor of dimethyl gallium azido-tert-butylamine($Me_2Ga(N_3)NH_2C(CH_3)_3$) with additional source of ammonia. The surface morphology, structural and optical properties of GaN thin films were analyzed for the deposition in the temperature range of $600^{\circ}C$ to $750^{\circ}C$. Electrical properties of deposited thin films were carried out by four point probe technique and home made Hall effect measurement. The effect of ammonia on the crystallinity, microstructure and optical properties of as-deposited thin films are discussed briefly. The crystalline quality of GaN thin film was improved with substrate temperature as indicated by XRD rocking curve measurement. Photoluminescence measurement shows broad emission around 350nm-650nm which could be related to impurities or defects.

• 방송공학회논문지
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• 제13권6호
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• pp.859-874
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• 2008

본 논문은 3차원 디스플레이 시스템에서 카메라의 기하 정보 및 참조 영상들의 깊이 맵 정보가 주어졌을 때, 다수의 중간 시점 영상을 실시간으로 생성하는 고속 영상 합성 기법을 제안한다. 기본적으로 본 논문에서는 영상 합성 기법의 모든 과정을 GPU에 서 병렬 처리함으로써 고속화 할 수 있었다. 병렬처리를 이용한 고속화 효율을 높이기 위해 최근 NVIDIA사에서 발표한 $CUDA^{TM}$를 이용하였다. 영상 합성을 위한 모든 중간 과정을 CUDA로 처리하기 위해 병렬구조로 변환하고, GPU 상의 고속메모리의 사용을 극대화하고, 알고리즘 구현을 최적화함으로써 고속화 효율을 높일 수 있었다. 결과적으로 본 논문에서는 양안 영상과 깊이 지도를 이용하여 가로 720, 세로 480 크기의 9개의 시점 영상을 0.128초 이내에 생성할 수 있었다.

• IEIE Transactions on Smart Processing and Computing
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• 제6권2호
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• pp.129-132
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• 2017

A conventional pulse oximeter has high power consumption; thus, its mobility is severely limited. In this paper, we discuss the drawbacks of the existing pulse oximeters and propose a new ultra-low-power pulse oximeter that supports wireless data transmission for remotely monitoring vital signs, such as peripheral capillary oxygen saturation (SpO2) and beats per minute (BPM). We could notably reduce power consumption by using a low-frequency single clock in all well-customized modules. Also, our device is publicly certified, and thus, possibly engaged in clinical trials for commercial use.

• 대한기계학회논문집B
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• 제30권1호
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• pp.82-86
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• 2006

Carbon nanotubes have attracted much attention as future mechanical and electronic materials. However, manipulating techniques are not well developed yet. Here we propose to use electrostatic drop-on-demand devices to eject micro-droplets containing micelle-suspended single-walled carbon nanotubes. A simple electrostatic force analysis and photographic studies of droplet ejection process are presented. The analytical analysis shows that semiconducting species have higher electrostatic force density. However, enrichment of specific electronic types is not clear at large size droplets produced in this study. A micro-scale jetting device is being produced to prove the suggested behavior.

• Carbon letters
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• 제28권
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• pp.1-8
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• 2018

Graphene is a single atomic layer of carbon atoms, and has exceptional electrical, mechanical, and optical characteristics. It has been broadly utilized in the fields of material science, physics, chemistry, device fabrication, information, and biology. In this review paper, we briefly investigate the ideas, structure, characteristics, and fabrication techniques for graphene applications in lithium ion batteries (LIBs). In LIBs, a constant three-dimensional (3D) conductive system can adequately enhance the transportation of electrons and ions of the electrode material. The use of 3D graphene and graphene-expansion electrode materials can significantly upgrade LIBs characteristics to give higher electric conductivity, greater capacity, and good stability. This review demonstrates several recent advances in graphene-containing LIB electrode materials, and addresses probable trends into the future.