• 한국발생생물학회지:발생과생식
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• 제10권2호
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• pp.115-125
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• 2006

본 실험은 생쥐 난자의 성숙과 생존에 미치는 selenium의 영향을 알아보고자 수행하였다. 난자의 성숙은 현미경을 통해 관찰하였으며, 핵막 붕괴(germinal vesicle breakdown, GVBD)와 극체 형성(polar body formation, PB)은 체외 배양 시작 후 각각 2.5, 13시간에 확인하였다. 난자의 생존은 72 시간동안 체외 배양하면서 형태학적 차이로 정상 난자와 비정상 난자를 판별하였다. 또한 각 단계별로 수집된 난자의 glutathione(GSH) 함량은 spectrophotometer를 사용하여 glutathione assay로 측정하였다. 결과는 다음과 같다; 저농도의 selenium($0.005\;{\mu}g/mL{\sim}0.5\;{\mu}g/mL$)은 핵막 붕괴율과 극체 형성률을 증가시켰지만, 고농도의 selenium($5\;{\mu}g/mL$)은 감소시켰다. 저농도의 selenium은 극체 형성 시기 난자의 생존율을 증가시켰지만, 고농도의 selenium은 대조군과 별 차이가 없었다. 저농도의 selenium은 난자내 GSH 함량을 높게 유지시켰지만, 고농도의 selenium은 GSH 함량을 감소시켰다. 또한 극체 형성 시기 난자내 GSH 함량은 핵막 붕괴 시기 난자에 비해 높았다. 본 실험으로 볼 때, 저농도의 selenium은 대사과정에서 발생하는 oxidative stress에 의한 손상을 감소시킴으로써 난자의 질적 향상을 도우며, 성숙률을 증가시키는 것으로 사료된다. 또한 저농도의 selenium은 난자내 GSH 함량을 증가시켜 극체 형성 난자의 생존율을 증가시키는 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권8호
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• pp.1082-1087
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• 2014

Thirty-two lambs were distributed in eight treatments under $2{\times}2{\times}2$ factorial experiment to compare the effects of two levels of selenium (0.2 to 5 mg/kg dry matter [DM]), sulphur (0.25% and 0.37%) and copper (8 and 25 mg/kg DM) levels on selenium concentration in liver and serum of lambs. A liver biopsy was done on all animals and blood samples were collected from the jugular vein prior to the beginning of the treatments. The blood was sampled every thirty days and the liver was sampled after 90 days, at the slaughter. Increasing differences were noticed during the data collection period for the serum selenium concentration, and it was found to be 0.667 mg/L in animals fed with 5 mg Se/kg DM and normal sulphur and copper concentrations in their diet. However, a three-way interaction and a reduction of selenium concentration to 0.483 mg/L was verified when increasing copper and sulphur concentration levels to 25 ppm and 0.37% respectively. The liver selenium concentration was also high for diets containing higher selenium concentrations, but the antagonist effect with the increased copper and sulphur levels remained, due to interactions between these minerals. Therefore, for regions where selenium is scarce, increasing its concentration in animal diets can be an interesting option. For regions with higher levels of selenium, the antagonistic effect of interaction between these three minerals should be used by increasing copper and sulphur dietary concentrations, thus preventing possible selenium poisoning.

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.757-760
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• 2000

The selenium status of beef cattle in the southern part of Thailand and the selenium concentration of soil and herbage consumed by those animals were investigated. Samples were collected from three areas with different soil types, namely, sandy soil, peat soil and laterite soil. The selenium concentration of soil, herbage and blood plasma showed a similar tendency; the values of laterite soil were higher than those of the other two areas. However, the selenium concentration of herbage of each pasture was lower than the NRC requirement, and that of blood plasma was not in the sufficient level. These data suggested that beef cattle raised in these areas were in the chronic selenium deficiency. We concluded that selenium supplementation is needed for the increase of animal productivity in the southern part of Thailand.

• 한국환경보건학회지
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• 제21권3호
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• pp.28-37
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• 1995

This study is performed to find out the effects of selenium against cadmium toxicity. The experimental mice were divided into 6 gruops such as control group, cadmium alone treatment group, selenium treatment groups and two simultaneous treatment groups of selenium and cadmium. Mice were given intraperitoneal administration with two dosage of sodium selenite such as 1.0 mg/kg, 2.5 mg/kg body weight and cadmium chloride was administered 3.0 mg/kg body weight. After giving the challenge dose, the concentration of cadmium and metallothionein and histopathological change of liver and kidney were determined. The results were summarized as follows on 1. The simultaneously administration of selenium and cadmium significantly more decreased cadmium concentration in kidney and iiver tissues compared to the administration of cadmium only(P<0.05). 2. The simultaneously administration of selenium and cadmium more increased metallothionein concentration compared to administration of cadmium only. 3. The simultaneously administration of selenium and cadmium more decreased cadmium concentration in urine compared to the administration of cadmium only. 4. When liver and kidney tissues were observed with optical microscope, no obvious changes were visible in those tissues.

• 대한화장품학회지
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• 제30권1호
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• pp.73-77
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• 2004

셀레늄을 함유하는 펩타이드(셀레늄 펩타이드)는 셀레늄을 함유하는 배지에서 효모를 배양함으로써 생산 하였다. 효모 단백질의 GPC 분석을 통해서 다양한 크기의 단백질 내 셀레늄 분포를 조사한 결과 셀레늄이 효모 내 단백질에 균일하게 분포하는 것을 확인하였다. 단백질당 셀레늄 양은 배지에 첨가한 셀레늄 양이 증가함에 따라 증가하였고 펩타이드 내의 셀레늄 함량이 높아질수측 항산화 효과 (glutathione peroxidase 유사 활성)가 증가하였다. 단백질 가수분해효소 XIV를 이용하여 여러 분자량의 펩타이드를 생산하였으며 평균 분자량을 GPC로 분석하였다 셀레늄 펩타이드의 glutathione peroxidase (GPx) 활성은 펩타이드의 분자량이 감소할수록 증가하였다. Sodium selenate은 sodium selenite에 비해 효모의 성장 저해를 적게 받았고 단백질 내 셀레늄 함량이 sodium selenite보다 높았다. 이 결과는 효모 배양에 의한 셀레늄 펩타이드의 생산의 가능성과 이를 이용한 항산화 제로서의 응용가능성을 보여주었다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1433-1437
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• 2007

Two field trials were carried out to evaluate the performance of a soluble glass copper, cobalt and selenium bolus for maintaining adequate levels of the three trace elements in yaks. Forty yaks were used in trial 1 and 60 yaks were used in trial 2. In each trial two commercial soluble glass boluses were administered to half of the yaks. Blood samples were taken from the jugular vein at day 0, 30, 60, 90 in trial 1 and at day 0, 45, 75 and 105 in trial 2. The samples were analysed for copper status (serum caeruloplasmin activity and copper concentration), cobalt status (serum vitamin $B_{12}$ concentration and cobalt concentration), selenium status (erythrocyte glutathione peroxidase activity and selenium concentration) and serum zinc concentration. The erythrocyte glutathione peroxidase activities, serum caeruloplasmin activities and serum vitamin $B_{12}$ concentrations for trial 1 and 2 were all significantly increased for the bolused yaks (p<0.001 or p<0.01) on all sampling days. The bolused yaks had a significantly higher selenium and copper status in serum than the control yaks on all sampling days in trial 1 and 2 (p<0.05 or p<0.01). There were no significant differences in zinc and cobalt concentrations between the bolused yaks and the controls.

• Animal Bioscience
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• 제36권3호
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• pp.484-491
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• 2023

Objective: The aim of this trial was to investigate the effect of supplementation and withdrawal of selenium-enriched kale sprouts (SeKS) on productivity and egg Se concentration of laying hens. Selenium from commercial Se-enriched yeast (SeY) was used as a comparative Se source. Methods: One-hundred and eighty 61-week-old laying hens were randomly divided into 5 treatment groups with 4 replicates (9 hens each) in a 2×2+1 Augmented Factorial Experiment in a completely randomized design. The experimental diets were basal diet, basal diet supplemented with 0.2 and 0.4 mg Se/kg from SeKS and SeY, respectively. The 8-week feeding trial was divided into 2 periods, namely the Se supplemental period (week 1 to 4) and the Se withdrawal period (week 5 to 8). Results: Productive performance, egg quality and egg Se concentration of laying hens were not affected by sources of Se (SeKS and SeY) during both, the Se supplemental and withdrawal periods. Egg production and egg Se concentration increased (p<0.05) with increasing levels of Se supplementation. The egg Se concentration increased and reached a peak 1 week after Se supplementation. However, concentration of Se in eggs of hens fed Se from both sources decreased rapidly from the second week of the Se withdrawal period to reach the same egg Se concentration of hens fed the basal diet by the fourth week of the Se withdrawal period. Conclusion: The efficacy of Se from SeKS on productivity and egg Se concentration in laying hens was comparable to commercial SeY. Thus, SeKS can provide an alternate organic Se source for production of Se-enriched eggs.

• Journal of Radiation Protection and Research
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• 제17권1호
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• pp.11-19
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• 1992

카드뮴 독성의 반응을 파악하고 효과적인 치료방법을 제시하고자 백서에 $CdCl_2$ 용액을 경구투여하여 혈중 $K^+,\;Ca^{2+}$ 농도, glutamate oxaloacetate transaminase(GOT), glutamate pyruvate transaminase(GPT) 활성 및 c-AMP 농도를 경시적으로 측정하였다. 카드뮴 용액을 단독투여하면 혈중 $K^+$ 농도에 비해 $Ca^{2+}$ 농도의 감소가 유의하였으며, GOT와 GPT 및 c-AMP의 농도는 증가하였다. 카드뮴을 함유한 selenium 병용투여군과 metallothionein 병용투여군에서는 카드뮴 단독투여군에 비하여 혈중 $Ca^{2+}$ 농도에 유의한 변화가 없었다. 그러나 카드뮴의 체내 오염시 관찰할 수 있는 독성지표로 혈중 GOT 및 GPT활성과 c-AMP 농도의 상승이 selenium에 의하여 현저하게 감소하나 metallothionein에 의하여는 감소효과가 없었으므로, 카드뮴 투여로 증가한 이들 해독지표에 대한 보상효과는 selenium에서 현저함을 알 수 있었다.

• 대한화학회지
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• 제47권4호
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• pp.363-369
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• 2003

효모를 최적 배양조건에서 키운후 $1.14{\times}10_-3 M$의 셀레늄을 배양액에 가하여 24시간 배양한 후 효모를 얻는다. 효모의 건조중량에 대하여 약 $0.1{\%}$(w/w)이상의 셀레늄을 함유하는 고농도의 셀레늄-효모를 얻었고 세포벽에 부착된 무기셀레늄은 세척후 MBRT과정으로 확인하였다. MBRT에서 15분 이상 푸른색이 지속되는 결과로 무기이온의 세포벽 흡착이 거의 없음을 확인하였다. 셀레늄이 함유된 효모를 초음파로 분해한 후 $80{\%}(NH_4)_2SO_4$ 용액을 이용하여 단백질을 부분 정제하였고 ICP-AES로 측정된 효모내의 셀레늄 농도와 비교하였을 때 약 $60.6{\%}$의 셀레늄이 단백질 내에 존재함을 확인하였다. 전기이동으로 단백질을 분리하여 확인한 결과 많은 양이 발현된 단백질 띠에서는 상대적으로 셀레늄의 농도가 높았다. 그중 47 kDa 단백질의 경우 69.5 ${\mu}$g Se/g의 농도로 가장 많은 함량을 보였다. 이 단백질을 PVDF 막에 electroblotting하여 분리하였고 이를 산으로 가수분해하여 얻어진 아미노산들을 PITC와 반응시켜 유도체를 얻었다. 아미노산유도체들을 HPLC로 분리 확인한 결과 셀레노메티오닌의 상대적인 비율이 총아미노산의 $2{\%}$로 얻어졌다. 이러한 셀레늄은 단백질과 킬레이트의 형태로 존재하는 것이 아니고 대부분이 셀레늄의 유기체인 셀레노메티오닌으로 효모 내에서 생합성 된다고 볼 수 있다.

• Journal of Microbiology and Biotechnology
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• 제20권7호
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• pp.1101-1106
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• 2010

Pulsed electric field (PEF) of 1Hz, 1.5 kV, and 1ms increased the activities of catalase and inulinase over the whole range of applied Se concentrations compared with the non-treated cultures. A significant effect of selenium concentration (in the range of 5-14 ${\mu}g/ml$) on both intra- and extracellular enzyme activities was noted. At a Se concentration of 10 ${\mu}g/ml$, the activities of intra- and extracellular inulinases and extracellular catalase in the PEF-treated cultures reached the maximum of 71 U/g d.m., 46 U/g d.m., and approx. 8 U/ml, respectively. The maximum activity of intracellular catalase of approx. 6 U/ml (with and without PEF) was recorded at 5 ${\mu}g$ Se/ml. Further increasing of selenium concentration caused a decrease in the activity of the enzymes.