Browse > Article

Production and Characterization of Selenium Peptide from Saccharomyces Cerevisiae  

김은기 (인하대학교 공과대학 생명화학공학부 생물공학과)
김영옥 (인하대학교 의과대학 미생물학교)
이정옥 (인하대학교 공과대학 생명화학공학부 생물공학)
이백석 (캘리포니아대학 생물공학과)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.30, no.1, 2004 , pp. 73-77 More about this Journal
Abstract
Selenium containing peptide was produced by culturing yeast with selenium, Selenium was broadly incorporated in the various size of proteins based on the GPC analysis of the total yeast protein. The ratio of selenium to protein increased with the concentration of added selenium in the culture medium. Antioxidant activity (glutathione peroxidase-like activity) was proportional to the concentration of selenium concentration in the peptide. Different size of proteins were obtained by hydrolyzing the total yeast protein by protease XIV. Average molecular weight of selenium peptide was analyzed by GPC. Glutathione peroxidase (GPx) activity of the selenium peptide increased as the size of peptide decreased. Sodium selenite had strong inhibition on the yeast growth than sodium selenate. The ratio of selenium to protein was higher with sodium selenate than with sodium selenite. These results showed the potentials of selenium peptide production by yeast cultivation.
Keywords
selenium peptide; yeast; GPx; antioxidant;
Citations & Related Records
연도 인용수 순위
  • Reference
1 A novel glutathione peroxidase mimic with antioxidant activity /
[ X.J.Ren;L.Q.Yang;J.Q.Liu;D.Su;D.L.You;C.P.Liu;K.Zhang;G.M.Luo;Y.Mu;G.L.Yan;J.C.Shen ] / Arch. Biochem. Biophys.   DOI   ScienceOn
2 Differential espression of selenoproteins by human skin cells and protection by selenium from UVB-radiation-induced cell death /
[ T.S.Rafferty;R.C.McKenzie;J.A.A.Hunter;A.F.Howie;J.R.Arthur;F.Nicol;G.J.Beckett ] / Biochem. J.   DOI
3 The selenoenzyme phospholipid hydroperoxide glutathione peroxidase /
[ F.Ursini;M.Maiorino;C.Gregolin ] / Biochim. Biophys. Acta.   DOI   ScienceOn
4 Production of organically bound selenium yeast by continuous fermentation /
[ A.Demirci;A.L.Pometto Ⅲ ] / J. Agric. Food. Chem.   DOI   ScienceOn
5 Monomethyl selenium-specific inhibition of MMP-2 and VEGF expression: Implications for angiogenic switch regulation /
[ C.Jiang;W.Jiang;C.Ip;H.Ganther;J.Lu ] / Mol. Carcinog.   DOI   ScienceOn
6 Generation of selenium-containing abzyme by using chemical mutation /
[ G.Luo;Z.Zhu;L.Ding;G.Gao;Q.Sun;Z.Liu;T.Yang;J.Shen ] / Biochem. Biophys. Res. Commun.   DOI   ScienceOn
7 Comparison of the effects of seleno-L-methionine, seleno-DL-methionine, and selenized yeast on reproduction of mallards /
[ G.H.Heinz;D.J.Hoffman ] / Environmental Pullution   DOI   ScienceOn
8 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase /
[ D.E.Paglia;W.N.Valentine ] / J. Lab. Clin. Med.
9 Adaptive antioxidant response protects dermal fibroblasts from UVA-induced phototoxicity /
[ C.Meewes;P.Brenneisen;J.Wenk;L.Kuhr;W.Ma;J.Alikoski;A.Poswig;T.Krieg;K.S.Kochanek ] / Free radic. biol. Med.   DOI   ScienceOn
10 Speciation of Selenoamino Acids and Organoselenium Compounds in Selenium-enriched Yeast using High-performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry /
[ S.M.Bird;P.C.Uden;J.F.Tyson;E.Block;E.Denoyer ] / J. anal. at. spectrom   DOI   ScienceOn
11 Glutathione peroxidases as peroxynitrite reductases /
[ H.Sies;L.O.Klotz;V.S.Sharov;R.Tamler;A.Assmann;K.Briviba ] / Pathophysiology
12 Thiols and selenium: protective effect on human skin fibroblasts exposed to UVA radiation /
[ N.Emonet;M.T.Leccia;A.Favier;J.C.Beani;M.J.Richard ] / J. photochem. photobiol. B,Biol.   DOI   ScienceOn
13 The biochemistry of selenium and the glutathione system /
[ G.E.Arteel;H.Sies ] / Environ Toxicol. Pharmacol.   DOI   ScienceOn