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Genetic and Pathogenic Characterization of Bacterial Wilt Pathogen, Ralstonia pseudosolanacearum (Ralstonia solanacearum Phylotype I), on Roses in Korea

  • Lee, Ingyeong;Kim, Yeong Son;Kim, Jin-Won;Park, Duck Hwan
    • The Plant Pathology Journal
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    • v.36 no.5
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    • pp.440-449
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    • 2020
  • The purpose of this study was to analyze the genetic and pathogenic characteristics of Ralstonia pseudosolanacearum in roses in Korea, and to examine the similarities and differences between Korean isolates and the first-reported European strains. Between 2017 and 2019, seventeen isolates from rose plants were identified as R. pseudosolanacearum using Ralstonia-specific primers. All 17 isolates were identified as race 1 using race-specific primers, and were confirmed as biovar 3 due to their ability to utilize carbon sources. Multiplex PCR using phylotype discriminating specific primers identified the 17 isolates as phylotype I. Sequevar comparison with reference sequevars using the sequences of the egl, mutS, and fliC genes, and only the egl gene, revealed that the strains evaluated in this study corresponded to sequevar I-33. The pathogenicity in roses differed depending on the rose cultivars. The different methods used for the genetic characterization of R. pseudosolanacearum indicate that the 17 rose bacterial wilt isolates had the same genetic characteristics. The lack of genetic variation in these isolates indicates their recent introduction from other countries (likely European countries). Therefore, appropriate quarantine and control measures should be taken in order to avoid further increases in the pathogenicity and/or secondary host range of R. pseudosolanacearum through genetic mutation.

Determination of the work function of the Ni thin films by using $\gamma-FIB$ system ($\gamma-FIB$ 장치를 사용한 Ni 박막의 일함수 결정)

  • 오현주;현정우;이지훈;임재용;추동철;최은하;김태환;강승언
    • Journal of the Korean Vacuum Society
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    • v.12 no.1
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    • pp.16-19
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    • 2003
  • Ni thin films on the p-InP (In) substrates were grown at room temperature by using the ion beam-assisted deposition. In order to determine the work function of the Ni thin films, the $\gamma$values were measured as functions of the acceleration voltages by using Ne, Ar, $N_2$. and Xe ion sources. The dependences of the values on various gases and on the acceleration voltages of the focused ion beam were obtained to determine the work function of the Ni thin films. The value of the work function of the Ni thin films grown on the p-InP (100) substrate was 5.8 eV ~ 5.85 eV. These results provide important information on the electronic properties of Ni thin films grown on p-InP (100) substrates at room temperature.

Geochemical Dispersion and Enrichment of Fluvial Sediments Depending on the Particla Size Distribution (입도분포에 따른 하상퇴적물의 지구화학적 분산 및 부화)

  • 이현구
    • Economic and Environmental Geology
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    • v.32 no.3
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    • pp.247-260
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    • 1999
  • Geochermical characteristics of the fluvial sediments deprnding on particle size distribution size were investigated in the respect of majir, minor and rare eath element chemisitry. Ratios of $Al_{2}O_{3}/Na_{2}O$ and $K_{2}O/Na_{2}O$ of the sediments show the homogeneous valus, and partly positive correlation with $SiO_{2}/Al_{2}O_{3}$, respecively. Characteristics of minor element ratios (V/Ni, Cr/V, Ni/Co and Zr/Hf)are within the lower and narrow range. Thesesuggested that sediment sources may be acidic to intermediate granitic rock, and may be explained by simple weathering and sedimentation. With increasing SiO2 contents, concentrations of $Al_{2}O_{3}$, $Fe_{2}O_{3}$, CaO and MgO decreased, but those of $K_{2}O$ and $Na_{2}O$ increased, Concentrations of Ba, Be, Cs, Cu, Li, Ni, Sr, V and Zr show comparatively normal negative and some positive trends. Compared with the mean composition of granite, concentrations of $Al_{2}O_{3}$, $Fe_{2}O_{3}$, MnO, CaO and MgO in the sediments of the study area were highly enriced. Among some minor and rare earth elements, concentrations of As, Cd, Cu, and V were enriched, but those of Be, Ce, Rb, Sc, Sr and Zn were depleted when compared with average composition of granite. By decreasing of particle size fractions, SiO2, Rb and Sr conterts decreased, but concentrations of $Al_{2}O_{3}$, $Fe_{2}O_{3}$, CaO, MgO, $TiO_{2}$, MgO, $P_{2}O_{5}$, Be, Cu, Hf, Pb, V and Zr increased. From the correlations between particle size fractions and element concenreations, some elements of $Fe_{2}O_{3}$, CaO, MgO, $P_{2}O_{5}$, Cu, Ni, Zn and Zr showed typical trends in the secondary contramination sediments. These trends are typically shown under 100 mesh fractions. It indicates that the fraction of minus 100 mesh is the optimum size fraction for geochemical and environmental survey.

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GIS Technology for Environmental Gelolgic Mapping (환경 지질도 작성을 위한 GIS 응용연구)

  • 김윤종;유일현;김원영;신은선
    • The Journal of Engineering Geology
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    • v.4 no.3
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    • pp.321-331
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    • 1994
  • Environmental geologic maps were produced on the cheong-Ju area using GIS technique. They are GIS maps on land management and regional land use planning. In the last year, the model of environmental geologic map was established, and the digital database was constructed by environmental and geotechnical data collected form various sources. The special maps for environmental geologic study were also pnoduced ; landslide hazard and risk map, cut & fill map, actual run-off map and engineering geological map. The maps are secondary models (sub-model) in order to create final environmental geologic map. Finally, Environmental Geologic Unit(EGU) was evaluated for regional land use planning and land management by EGIS(Environmental Geologic Inforafion System). This unit is very important in order to assess environmental geologic impact on large construction works and detailed road design etc.

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The Relationships between Health Behaviors, Mental Health and Smoking among Korean Adolescents (한국 청소년의 건강행태 및 정신건강과 흡연의 관련성)

  • Song, Hye-Young;Doo, Eun Young;Choi, Sujin
    • The Journal of the Korea Contents Association
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    • v.17 no.7
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    • pp.557-570
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    • 2017
  • This study, as a secondary analysis research, bases its source upon on raw data from the on-line survey of the Korean adolescents' health behaviors in 2015. This study was conducted to clarify the relevance of Korean adolescents' smoking with their health behaviors and mental health, and thus further to provide basic sources of effective education for their smoking prevention and for program development. The results of the study showed that Korean adolescents' smoking was significantly affected by the adolescents' sex, grades, school achievements, types of schools, allowances, economic status, parents' educational background (p<0.001). Meanwhile, smoking based on the characteristics of health behaviors was significantly different according to alcohol consuming experience, families' and friends' smoking, physical activity and subjective health status, and BMI (p<0.001). Smoking characterized by mental health showed the significant difference in their stress recognition, subjective sleep patterns, depressed experiences, suicidal ideation, and subjective sense of happiness (p<0.001). Based on all the factors regarding youth smoking, there should be development and provision of educational program for smoking prevention and cessation, including healthy behavior and mental health improvement for Korean adolescent.

Comparison of effectiveness for fluoroscopic cervical interlaminar epidural injections with or without steroid in cervical post-surgery syndrome

  • Manchikanti, Laxmaiah;Malla, Yogesh;Cash, Kimberly A;Pampati, Vidyasagar;Hirsch, Joshua A
    • The Korean Journal of Pain
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    • v.31 no.4
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    • pp.277-288
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    • 2018
  • Background: Neck and back pain are leading sources of disability placing substantial burden on health care systems. Surgical interventions in managing chronic neck pain secondary to various disorders continue to increase. Even though surgical interventions are effective, a significant proportion of patients continue to have symptomatology and develop cervical post-surgery syndrome. This study was performed to know the effectiveness of cervical interlaminar epidural injections with or without steroids. Methods: The effectiveness of fluoroscopic cervical interlaminar epidural injections in post-surgery syndrome was evaluated in a randomized, active controlled trial. The study population included 116 patients assigned to 2 groups. Group 1 received cervical interlaminar epidural injections with local anesthetic alone and Group 2 received injection with local anesthetic and steroids. The main outcomes were defined as significant improvement (greater than 50%) of pain relief using the numeric rating scale and/or functional status improvement using the Neck Disability Index (NDI). Results: Both groups had similar results with significant improvement (${\geq}50%$ pain relief and functional status improvement) in 69% of the patients in Group I, whereas, in Group II, 71% of the patients showed significant improvement at the end of 2 years. During a 2-year period, the average number of procedures was 5 to 6, with an average of approximately 12 weeks of significant improvement per procedure. Conclusions: Fluoroscopic cervical interlaminar epidural injections administered in cervical post-surgery syndrome using local anesthetic, regardless of the use of steroids, may be effective in approximately 70% of the patients at 2-year follow-up.

Study on Characterization of Hydrophilic and Hydrophobic Fractions of Water-soluble Organic Carbon with a XAD Resin (XAD 수지에 의한 친수성 및 소수성 수용성 유기탄소의 특성조사)

  • Jeong, Jae-Uk;Kim, Ja-Hyun;Park, Seung-Shik;Moon, Kwang-Joo;Lee, Seok-Jo
    • Journal of Korean Society for Atmospheric Environment
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    • v.27 no.3
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    • pp.337-346
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    • 2011
  • 24-hr integrated measurements of water-soluble organic carbon (WSOC) in PM2.5 were made between May 5 and September 25, 2010, on a six-day interval basis, at the Metropolitan Area Air Pollution Monitoring Supersite. A macro-porous XAD7HP resin was used to separate hydrophilic and hydrophobic WSOC. Compounds that penetrate the XAD7HP column are referred to hydrophilic WSOC, while those retained by the column are defined as hydrophobic WSOC. Laboratory calibrations using organic standards suggest that hydrophilic WSOC includes lowmolecular aliphatic dicarboxylic acids and carbonyls with less than 4 or 5 carbons, amines, and saccharides. While the hydrophobic WSOC is composed of compounds of aliphatic dicarboxylic acids with carbon numbers larger than 4~5, phenols, aromatic acids, cyclic acid, and humic-like Suwannee River fulvic acid. Over the entire study period, total WSOC accounted for on average 48% of OC, ranging from 32 to 65%, and hydrophilic WSOC accounted for on average 30.5% (9.3~66.7%) of the total WSOC. Based on the previous results, our measurement result suggests that significant amounts of hydrophobic WSOC during the study period were probably from primary combustion sources. However, on June 9 when 1-hr highest ozone concentration of 130 ppb was observed, WSOC to OC was 0.61, driven by increases in the hydrophilic WSOC. This result also suggests that processes, such as secondary organic aerosol formation, produce significant levels of hydrophilic WSOC compounds that add substantially to the fine particle fraction of the organic aerosol.

Concentrations and Characteristics of Carbonaceous Compounds in PM10 over Seoul: Measurement between 2006 and 2007 (서울시 대기 중 미세먼지 내 탄소성 입자의 농도 및 특성 : 2006~2007년 측정결과)

  • Choi, Na Rae;Lee, Ji Yi;Jung, Chang Hoon;Lee, Sun Yong;Yi, Seung Muk;Kim, Yong Pyo
    • Journal of Korean Society for Atmospheric Environment
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    • v.31 no.4
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    • pp.345-355
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    • 2015
  • Carbonaceous compounds in the atmospheric particulate matter with an aerodynamic diameter of less than or equal to a nominal $10{\mu}m$ ($PM_{10}$) were analyzed for the samples collected during the period of August 2006 to August 2007 at Jongro in Seoul. A total 18 dicarboxylic acids (DCAs) and levoglucosan, as well as organic carbon (OC), elemental carbon (EC), and water soluble organic carbon (WSOC), were analyzed. Distinctive seasonal patterns of the concentrations of OC, EC, and WSOC including levoglucosan were observed with the highest concentrations in winter and the lowest concentrations in summer. In addition, OC, WSOC, and most of DCAs showed also higher concentration in summer than in winter. Using the seasonal patterns and relevant indicative ratios (WSOC/EC and $OC_{sec}/OC_{tot}$) of the carbonaceous compounds, it was verified that (1) primary emission sources were elevated in winter, and (2) the formation of secondary OC increased due to the prompted photochemical reaction in summer. Results from this study also suggest that some organic compounds were likely attributed to longrange transport.

Spatial and Temporal Variations of Atmospheric Concentrations of Carbonyl Compounds in Seoul Metropolitan Area (수도권 지역 대기 중 카보닐화합물의 시.공간적 농도 분포 특성)

  • Seo, Young-Kyo;Jeong, Eun-Hui;Seo, Seok-Jun;Hwang, Yun-Jeong;Han, Jin-Seok;Bae, Sung-Ok
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.2
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    • pp.206-219
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    • 2008
  • The purpose of this study is to evaluate concentrations of carbonyl compounds during summer season in Seoul metropolitan area. The air samples were collected at 7 sites in this area from June 2001 to June 2003. The carbonyl compounds were analyzed by DNPH/HPLC method. The analytical method applied in this study showed good repeatability, linearity, and sensitivity. The most abundant carbonyl was formaldehyde (average 4.48 ppb), and followed by acetone, acetaldehyde, methyl ethyl ketone, butyraldehyde, propionaldehyde and benzaldehyde, respectively. Concentrations of carbonyl compounds in June were higher than those in August. There was not only higher solar radiation but also higher ozone concentration in June than in August. As a result o photochemical reactions, carbonyl compounds from both primary and secondary sources are likely to contribute to the formation of ozone. The contributions to photochemical ozone creation of two carbonyl compounds such as formaldehyde and acetaldehyde were estimated to be about 70%. Ratios of formaldehyde to acetaldehyde in this study ranged from 1.13 to 4.26, which are generally equivalent levels to those of other urban areas in domestic and foreign countries.

Experimental Techniques for Evaluating the Success of Restoration Projects

  • Robinson, George R.;Handel, Steven-N.l;Mattei, Jennifer
    • The Korean Journal of Ecology
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    • v.25 no.1
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    • pp.1-7
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    • 2002
  • The ecological background of a restoration project is complex and difficult to betermine without experimentation. A useful context for experiments is the well-studied process of natural succession, because the factors that drive or inhibit succession are also at work during reclamation (a form of primary succession) and restoration (which often resembles secondary succession). Using experimental studies on urban wasteland reclamation, we have tested for factors that stimulate or inhibit succession during early phases of woodland development in the Northeastern United states. The emphasis has been on mutualisms (seed dispersal, pollination, and mycorrhizae) and microsite limitations in the recruitment, growth, and reproduction of woody plants. Using plantings of seeds, seedlings, and clusters of reproductively mature plants on abandoned landfills, we have observed that (1) soil microsite deficiencies lead to very poor germination (<0.1$\%$) and seedling survival (<0.01$\%$) of most native species; (2) seed dispersal by birds is a significant and reliable source of woody plant recruitment; however (3) proximity effects are strong, with most (up to 95$\%$) of seed rain falling in the vicinity of planted clusters that are closest to putative seed sources; and (4) remnant natural woodlands are critical components of the recruitment process. To emphasize the last point, in one case, we found that the destruction of approximately 50$\%$ of nearby natural woodland vegetation led to a commensurate decline in seed rain. In another case, we found that the species richness of recruits was strictly limited by the species composition of nearby source plant communities, with no evidence of community enrichment by long distance dispersal over 5 years. We conclude from these results that the size and proximity of remnant natural populations are critical considerations when planning reclamation and restoration programs that rely on natural successional processes.