• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 공동 춘계학술대회
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• pp.91.2-91
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• 2001

Allelic ladders ; DNA profiling ; Koreans ; STRs ; Y-chromosome haplotypes

• 사상체질의학회지
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• 제21권1호
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• pp.227-236
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• 2009

1. Objectives As a basic trial for identification of Sasang constitutional gene marker, we genotyped and analysed statistical relationships of STR(short tandem repeat) alleles and its distribution in each constitution. 2. Methods After obtaining basic constitutional data with questionnaire (QSCC II), decision of constitution was made by 3 different constitution specialists' diagnosis, and only the samples of specialists' agreement of each constitution by discussion were taken into this research. Using multiplex PCR kit, total 146 constitutional samples were amplified in 16 autosomal STR marker, genotyped, and analysed statistically. Among 16 markers, 15 were analysed in this study excluding the amelogenin marker is used for in gender identification. 3. Results and Conclusions It is difficult to determine the relationship between constitution and STR marker as the sample size is small, however, Penta D and vWA were shown to be related statistically with constitution. It has been know that STRs has no genetic informations, however there are some recent research results showing STRs as a regulatory element, relationship between microsatellite instability and repeat number and size, and post-transcriptional sigualing. STRs which is not known about its function currently, are proposed to have function and/or regulatory activities anyhow with Sasang constitution. It is believed that the results of this study can halp determine and deatify the markers related to Sasang Constitutional Medicien.

• 대한의생명과학회지
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• 제17권2호
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• pp.151-155
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• 2011

Short tandem repeats (STRs) loci are the genetic markers used for forensic human identity test. With multiplex polymerase chain reaction (PCR) assays, STRs are examined and measured PCR product length relative to sequenced allelic ladders. In the repeat region and the flanking region of the commonly-used STR may have DNA sequence variation. A mismatch due to sequence variation in the DNA template may cause allele drop-out (i.e., a "null" or "silent" allele) when it falls within PCR primer binding sites. The STR markers were co-amplified in a single reaction by using commercial PowerPlex$^{(R)}$ 16 system and AmpFlSTR$^{(R)}$ Identifiler$^{(R)}$ PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI PRISM$^{(R)}$ 3100 Genetic Analyzer with capillary electrophoresis. The GeneMapper$^{TM}$ ID software were used for size calling and analysis of STR profiles. Here, this study described a forensic human identity test in which allelic drop-out occurred in the STR system D18S51. During the course of human identity test, two samples with a homozygous (16, 16 and 21, 21) genotype at D18S51 locus were discovered using the PowerPlex$^{(R)}$ 16 system. The loss of alleles was confirmed when the samples were amplified using AmpFlSTR$^{(R)}$ Identifiler$^{(R)}$ PCR amplification kit and resulted in a heterozygous (16, 20 and 20, 21) genotype at this locus each other. This discrepancy results suggest that appropriate measures should be taken for database comparisons and that allele should be further investigated by sequence analysis and be reported to the forensic community.

• Animal cells and systems
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• 제5권3호
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• pp.237-241
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• 2001

Three STR loci (STRX1[AGAT]$_{n}$, HPRTB[AGAT]$_{n}$ and ARA[AGC]$_{n}$) on X chromosome and three other STR loci (DYS390[CTG(A)T]$_{n}$, DYS392[ATT]$_{n}$ and DYS39［GATA]$_{n}$) on Y chromosome were analyzed in 154 unrelated healthy Korean subjects. Four loci (STRX1, HPRTB, DYS390 and DYS393) were amplified by quadruplex polymerase chain reaction (PCR) using fluorescent labeled primers (FLP). ARA and DYS392 were amplified separately using single PCR, similarly by using FLP. They were then run in an automated DNA sequencer and were analyzed with Genescan software. We found 7 alleles (308-332 bp) in STRX1, 7 alleles (275-299 bp) in HPRTB, 16 alleles (252-315 bp) in ARA, 6 alleles (203-223 bp) in DYS390, 7 alleles (245-263bp) in DYS392 and 5 alleles (116-132 bp) in DYS393. The ＊13(34%), ＊13(5l%), ＊23 (l8%), ＊23(50%), ＊14(39%) and ＊13(40%) alleles were observed to be the highest frequencies of STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393, respectively. The detection of STR loci on sex chromosomes by quadruplex PCR allows simple determination of sex and identification of an individual. individual.

• 대한의생명과학회지
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• 제29권4호
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• pp.302-313
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• 2023

Y chromosomal short tandem repeat (Y-STR) markers have been developed continuously to complement forensic DNA analyses and population genetic studies. Initially, we collected data from previously reported Korean population Y-STR haplotype studies on 1133 individuals. We then conducted a marker expansion analysis using a dataset from the Y-STR Haplotype Reference Database (YHRD), covering up to 29 Y-STRs, referred to as Ymax. Additionally, we examined the impact of rapidly mutating (RM) Y-STRs included in this expanded marker set on the discrimination capacity. We observed that marker expansions both with (0.9896), and without (0.9510), RM Y-STR improved the discrimination capacity. Subsequently, we focused on 16 individuals belonging to seven distinct groups sharing identical haplotypes. These particular haplotypes had been previously identified among 476 unrelated males using 23 Y-STR markers from the PowerPlex^® Y23 System. We expanded the marker panel up to Ymax to explore how discrimination improved with an expansion of Y-STR markers for these 16 individuals. Among the expanded markers, DYS627, which had high discriminatory power, had a high mutation rate (1.10 × 10^-2) and high gene diversity (0.83). In contrast, DYF387S1 displayed high gene diversity (0.95) but a relatively low mutation rate (2.80 × 10^-3). We propose that these findings will be valuable in the selection of suitable Y-STR markers, depending on the objectives of forensic analyses. Additionally, the presence of frequently observed Y-haplotypes in Korean population will facilitate statistical interpretation in Y-STR DNA profiling.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권3호
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• pp.169-173
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• 2000

On human chromoscomes, a short sequence of DNA is known to repeat a number of times. These are called variable number of tandem repeat (VNTR) or short tandem respeat (STR) which has a short core. VNTR and STR are used in the filed of forensic science, evolution, and anthropology. In this work, we examined allele frequencies of one VNTR (YNZ22) and three STRs (NeuR, D21S11, Humth01) in a korean population sample by polymerase chain reaction (RCP) followed by high-resolution polyacrylamide gel electro-phoresis (PAGE) with silver stain. Subsequently, the polymorphism information content (PIC) was calculated : the hifhest PIC was observed in the NeuR locus (0.95680) and lowest in the Humth01 locus (0.75809).

• 한국정보처리학회논문지
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• 제6권7호
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• pp.1877-1883
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• 1999

ATM 수신 단말기에서 원래 송신 단말기의 클럭과 동일한 주파수를 가져야 하는, 클럭의 동기에 관하여 연구하였다. 이를 위하여 ITU-T에서 권고하고 있는 표준 방식인 SRTS 방식과 이 방식에서 발생되는 지터를 분석하였다. STRS 지터의 전력 스펙트럼과 rms값이 이론적으로 계산되었다. 분석 결과, T1 1.544MHz의 소스 신호의 경우 평균적인 rms값은 32.63ns이고, E4 139.264MHz 신호의 경우, 0.15ns인 것으로 밝혀졌다.

• 지능정보연구
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• 제14권2호
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• pp.179-192
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• 2008

본 논문은 금융기관을 이용한 자금세탁 및 불법적인 외화유출 방지를 목적으로 자금세탁 관련 혐의거래보고 등 금융정보를 수집하여 심사하는 금융정보분석원에 지식기반시스템을 도입한 사례연구이다. 한정된 심사인력으로 기하급수적으로 증가하는 협의거래보고에 효과적으로 대응하기 위하여 지식기반시스템의 도입은 필수적이라고 할 수 있다. 이렇게 구축된 지식기반시스템은 보고된 혐의거래를 여과(filtering)하여 자금세탁혐의가 인정된 정보만을 수사기관에 제공하는 심사 및 분석 업무의 효과성과 효율성을 극대화시킨다. 특히, 금융정보분석원은 여러 금융기관들로부터 보고된 혐의거래정보와 심사분석과정에서 유관기관으로부터 수집된 여러 종류의 정보가 집중되기 때문에 축적된 정보를 체계적으로 관리 및 활용할 수 있는 지식 베이스의 구축이 더욱 필요하다. 금융정보분석원은 많은 정보가 집중되는 만큼 축적된 데이터를 활용하여 자금세탁 관련 지식을 창출하는 업무를 수행해야만 하는 의무도 가지고 있기 때문이다. 이를 위하여 금융정보분석원의 심사분석시스템이 자금세탁방지를 위한 지식의 창출과 지식의 관리 측면까지 고려된 전체적인 프레임워크 하에서 지식기반시스템으로써의 토대를 마련하였다는 점에서 의의가 크다고 할 수 있다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.483-483
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• 2013

Latral Composition Modulation (LCM)으로 성장한 InP/GaP 초격자(Superlattice)의 선편광된 광발광(Photoluminescence) 특성을 저온(5 K)에서 측정하였다. LCM 기법은 z-축 방향으로 InP와 GaP를 단층 초격자(monolayer supperlattice)로 성장하는 과정에서 strain에 의해 x-y 평면으로 초격자가 형성되는 특별한 경우이다. 이렇게 성장된 LCM 초격자의 경우 In-rich 영역과 Ga-rich 영역이 교차로 성장되는 구조를 가지며 가전자대역(valence band)에서 무거운 양공과 가벼운 양공의 band mixing 이 일어나게 되어 선평광된 발광특성을 가진다. 우리는 저온 발광실험에서 In-rich 영역과 Ga-rich 영역의 재결합에 의해 나타나는 두 개의 독립된 전이 피크를 측정하였다. 이 두 피크는 [110] 방향의 편광에서 발광 강도가 최대치를 가지며 [1-10] 방향에서 최소값을 가짐을 보였다. 이때 전이 에너지의 경우 [110] 방향에서 [1-10] 방향으로 편광이 바뀔 때 Ga-rich 영역의 전이의 경우 적색편이를 나타낸 반면 In-rich 영역의 경우 청색편이를 보이는 현상을 발견하였다. 이러한 상반된 편이 현상은 서로 다른 3족 물질의 영역에 따라 격자 상수가 바뀌며 tensile strss와 compressive stress에 따른 가전자 대역의 band mixing 변화에 기인하는 것으로 여겨진다.

• 한국보육지원학회지
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• 제16권5호
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• pp.69-86
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• 2020

Objective: The purpose of this research was to examine the prediction models of conflict and intimacy in teacher-child relationships based on decision tree analysis. Methods: The participants were 297 preschool children from ages three to five including 166 boys and 131 girls. Teacher-child relationships were measured by the Student-Teacher Relationship Scale(STRS). Physical aggression, relational aggression, social withdrawal, and prosocial behaviors were measured by teacher ratings. Moreover, ADHD-RS(Attentive Deficit Hyperactivity Disorder Rating Scale) was used to measure ADHD. The data was analyzed with decision tree analysis. Results: According to the prediction model for teacher-child conflict, the significant predictors were physical aggression and social withdrawal. According to the prediction model for teacher-child intimacy, the significant predictors were prosocial behaviors and relational aggression. However, children's age, gender and ADHD were not significant predictors. Conclusion/Implications: The findings suggest that social behaviors may be closely related with teacher-child relationships for preschool children. Based on the results of this study, intervention suggestions were made.