Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Analysis of the Short Tandem Repeat Loci for STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393 in Koreans

  • Seol, Hye-Won (Department of Biology, College of Natural Sciences, Sungshin Women's University) ;
  • Zaw Tun (Department of Legal Medicine, Medical School, Osaka University) ;
  • Katsuya Honda (Department of Legal Medicine, Medical School, Osaka University) ;
  • Shogo Misawa (Department of Legal Medicine, School of Medicine, Tsukuba University) ;
  • Park, Kyung-Sook (Department of Biology, College of Natural Sciences, Sungshin Women's University)
  • Published : 2001.09.01
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Abstract

Three STR loci (STRX1[AGAT]$_{n}$, HPRTB[AGAT]$_{n}$ and ARA[AGC]$_{n}$) on X chromosome and three other STR loci (DYS390[CTG(A)T]$_{n}$, DYS392[ATT]$_{n}$ and DYS39［GATA]$_{n}$) on Y chromosome were analyzed in 154 unrelated healthy Korean subjects. Four loci (STRX1, HPRTB, DYS390 and DYS393) were amplified by quadruplex polymerase chain reaction (PCR) using fluorescent labeled primers (FLP). ARA and DYS392 were amplified separately using single PCR, similarly by using FLP. They were then run in an automated DNA sequencer and were analyzed with Genescan software. We found 7 alleles (308-332 bp) in STRX1, 7 alleles (275-299 bp) in HPRTB, 16 alleles (252-315 bp) in ARA, 6 alleles (203-223 bp) in DYS390, 7 alleles (245-263bp) in DYS392 and 5 alleles (116-132 bp) in DYS393. The ＊13(34%), ＊13(5l%), ＊23 (l8%), ＊23(50%), ＊14(39%) and ＊13(40%) alleles were observed to be the highest frequencies of STRX1, HPRTB, ARA, DYS390, DYS392 and DYS393, respectively. The detection of STR loci on sex chromosomes by quadruplex PCR allows simple determination of sex and identification of an individual. individual.

Keywords

References

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