• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.344-352
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• 2010

To investigate the mating system of clones in the seed orchard of Japanese red pine, parameters of mating system, such as outcrossing rates, number of potential pollen contributors, and degree of pollen contamination, were estimated in the seed orchard of Japanese red pines on the basis of DNA data including 4 nSSR and 6 cpSSR markers. Estimates of outcrossing rates were ranged from 94.9 to 100% with an average of 98.9% on the basis of the analysis of cpSSR haplotypes. They were ranged from 90.3% to 100% with an average of 95.9% on the basis of the analysis of nSSR genotypes. However, cross checking of both DNA markers revealed that the seeds presumed to be products of self pollination were turned out to be generated by pollination between mother tree and other tree (i.e., 100% of cumulative outcrossing rate). Estimates of pollen contamination ranged from 43.6% (Gangwon-10) to 56.4% (Gangwon-12) with the average of 48.9%. On the basis of pooled cpSSR haplotype of each seed, maximum number of 21 pollen contributors were verified from the seeds reproduced by Kyungbuk-38. Minimum number of 13 pollen contributors were verified in Gangwon-10. Mean of 16.2 pollen contributors were verified from a total of 5 mother trees. In conclusion, considering pretty high outcrossing rates between clones within a seed orchard, it may be expected that a fairly good genetic potential of the seeds, produced in '77 plot of the seed orchard of Japanese red pines at Anmyeon island, may be guaranteed. Observed results from the analysis of mating system of Japanese red pines in a '77 plot of the seed orchard may also provide useful information for the establishment and management of the seed orchard of the progressive generation.

• Horticultural Science & Technology
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• v.34 no.1
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• pp.142-153
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• 2016

The present study investigated identification of cultivars through phylogenetic analysis of 108 Citrus varieties and related cultivars using simple sequence repeat (SSR) markers. Two hundred three SSR primer pairs were used to detect polymorphic markers among 8 Citrus cultivars consisting of 4 mandarins, 1 orange, 1 tangor, 1 tangelo, and 1 pumelo. Eighteen SSR primer pairs were reproducible and showed highly polymorphic alleles. These markers were applied to assess genetic variations of the 108 varieties. Each marker detected 5-14 alleles, with an average of 9.28. The polymorphism information content varied from 0.417 to 0.791 with an average of 0.706. Cluster analysis with SSR markers resulted in 13 major groups reflecting cultivar types and pedigree information. Twelve orange cultivars in the $I-1^{st}$ sub-cluster and 23 mandarin cultivars in the $II-1^{st}$ sub-cluster, respectively, were not discriminated using the SSR markers. This could be due to narrow genetic backgrounds originated through bud mutation or nucellars seedlings. The SSR profile database of Citrus cultivars will be useful as a tool for protection of plant breeders' intellectual property rights in addition to assessing genetic diversity in Citrus cultivars and germplasms.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.405-412
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• 2011

Knowledge of genetic diversity and genetic relationships among inbred lines gives a significant impact on the selection of parental lines for hybrid maize varieties. Genetic diversity and genetic relationships among 86 popcorn inbred lines were analyzed using 50 SSR markers distributed over the whole genome. A total of 256 alleles were identified at all the SSR loci with an average of 5.1 and a range between two and sixteen per locus. The gene diversity values varied from 0.21 to 0.831 with an average of 0.579. The cluster tree generated using the described SSR markers recognized three major groups at 35.8% genetic similarity. Groups I, II, III respectively included 40, 39 and 7 inbred lines. The present study indicates that the SSR markers chosen for this analysis are effective for the assessment of genetic diversity and genetic relationships among 86 popcorn inbred lines in Korea.

• Journal of Positioning, Navigation, and Timing
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• v.6 no.2
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• pp.79-86
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• 2017

Precise Point Positioning-Real Time Kinematic (PPP-RTK) refers to a technology that combines PPP with network-RTK in which a user does not directly receive observed data from a reference station but receives State-Space Representation (SSR) messages corrected for error components from a central processing station through Networked Transport of RTCM via Internet Protocol (NTRIP) or Digital Multimedia Broadcasting (DMB) for purposes of positioning. SSR messages, which refer to corrections used in PPP-RTK, are generated by a central processing station using real-time observed data collected from reference stations and account for corrections needed due to the ionosphere, troposphere, satellite orbital errors, satellite time offsets, and satellite biases. This study used a type of SSR message provided in South Korea, known as Korea-SSR (K-SSR), to implement a PPP-RTK algorithm based on code-pseudorange measurements and validated its accuracy within the reference station network. In order to validate the accuracy of the implemented algorithm outside of the network, the K-SSR was extrapolated and applied to positioning in reference stations in Changchun, China (CHAN) and Japan (AIRA). This also entailed a quantitative evaluation that measured improvements in accuracy in comparison with point positioning. The results of the study showed that positioning applied with extrapolated K-SSR correction data was more accurate in both AIRA and CHAN than point positioning with improvements of approximately 20~50%.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.243-263
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• 2017

This study is to establish the varietal discrimination based on DNA profiling of different varieties of rice. We examined the genetic distance among Korean rice varieties using allele frequencies and a genetic diversity analysis with Simple Sequence Repeats (SSRs) markers. The analysis of the genetic diversity and genetic relationships of 243 Korean rice varieties was varied out using 20 SSRs markers. A total of 268 alleles were detected, ranging from 6 to 32, with an average of 13.45 alleles per locus, and and average of gene diversity (GD) of 0.556. Seven SSR markers were selected as key markers for discrimination among the Korean rice varieties. Concerning the results, 243 varieties (100%) were discriminated among by using acrylamide gel and fragment analyzer-based markers. In conclusion, this study provides useful basic data that can be utilized concerning Korean rice varieties breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing the diversity of Korean rice varieties.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.189-195
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• 2006

We investigated the genetic variation in Stewartia koreana Nakai by examining 61 I-SSR amplicons in 120 individuals distributed among six natural populations in Korea. The overall percentage of polymorphic I-SSR amplicons was 81.9% and mean number of amplicons per I-SSR primer was 12.2. Levels of genetic diversity within 6 populations were similar each other[Shannon's Index $0.358{\sim}0.467$(mean: 0.407)]. The Mt. Obong population had the highest level of genetic diversity and was most distinctive from the other populations. Most variation existed among individuals within population(88.2%). Genetic differentiation among populations(${\phi}_{ST}$) was 0.118. The UPGMA dendrogram based on the genetic distance failed in showing decisive geographic relationships.

• Molecules and Cells
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• v.19 no.3
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• pp.428-435
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• 2005

This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.

• Journal of Advanced Navigation Technology
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• v.26 no.6
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• pp.418-426
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• 2022

Unlike OSR(observation space representation), the SSR(state space representation) augmentation system is suitable for a one-way broadcasting service because it provides the same corrections to all users in the service area. Due to this advantage, several GNSS(global navigation system) systems such as Galileo, BDS(beidou navigation satellite system), QZSS(quasi zenith satellite system) are establishing PPP (precise point positioning)/PPP-RTK precision positioning services based on SSR messages. Therefore, in this paper, we try to understand the trends of satellite-based PPP/PPP-RTK correction services by analyzing the system configurations, characteristics, and precise positioning performance of satellite-based SSR correction broadcasting services.

• KIEE International Transactions on Power Engineering
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• v.3A no.3
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• pp.139-147
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• 2003

The paper presents a novel approach of constructing the state matrix of the multi-machine power system for SSR (subsynchronous resonance) analysis using the linearized equations of individual devices including electrical transmission network dynamics. The machine models in the local d-q reference frame are integrated with the network models in the common R-I reference frame by simply transforming their output equations into the R-I frame where the transformed output is used as the input to the network dynamics or vice versa. The salient feature of the formulation is that it allows for modular construction of various component models without rearranging the overall state space formulation. This universal SSR small signal stability program provides a flexible tool for systematic analyses of SSR small-signal stability impacts of both conventional devices such as generation systems and novel devices such as power electronic apparatus and their controllers. The paper also presents its application results to IEEE benchmark models.

• Proceedings of the KIEE Conference
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• 2000.11a
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• pp.89-91
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• 2000

This paper presents a new method for representing SSR(Subsynchronous Resonanace) Phenomena using 3-D computer graphics animation. A virtual power system simulation environment was made for simulation and visualization of SSR phenomena. It is anticipated that the proposed method would be helpful for the operator training as well as power system education.