Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Discrimination of Korean rice varieties as revealed by DNA profiling and its relationship with genetic diversity

한국 육성 벼 품종의 DNA profiling에 의한 유전적 다양성 분석 및 품종 판별

  • Kim, Me-Sun (Department of Crop Science, Chungbuk National University) ;
  • Song, Jae-Young (Department of Crop Science, Chungbuk National University) ;
  • Kang, Kwon-Kyoo (Department of Horticultural Life Science, Hankyong National University) ;
  • Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
  • 김미선 (충북대학교 식물자원학과) ;
  • 송재영 (충북대학교 식물자원학과) ;
  • 강권규 (한경대학교 원예생명과학과) ;
  • 조용구 (충북대학교 식물자원학과)
  • Received : 2017.09.22
  • Accepted : 2017.09.25
  • Published : 2017.09.30
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Abstract

This study is to establish the varietal discrimination based on DNA profiling of different varieties of rice. We examined the genetic distance among Korean rice varieties using allele frequencies and a genetic diversity analysis with Simple Sequence Repeats (SSRs) markers. The analysis of the genetic diversity and genetic relationships of 243 Korean rice varieties was varied out using 20 SSRs markers. A total of 268 alleles were detected, ranging from 6 to 32, with an average of 13.45 alleles per locus, and and average of gene diversity (GD) of 0.556. Seven SSR markers were selected as key markers for discrimination among the Korean rice varieties. Concerning the results, 243 varieties (100%) were discriminated among by using acrylamide gel and fragment analyzer-based markers. In conclusion, this study provides useful basic data that can be utilized concerning Korean rice varieties breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing the diversity of Korean rice varieties.

벼 품종의 DNA profiling을 위하여 SSR 마커를 활용하여 한국 벼의 품종판별 기술 확립을 위하여 국내에서 육성된 벼 243개 품종에 대하여 최종 선발된 7개의 SSR 마커(RM21, RM257, HsSSR01-52, RM333, RM580, RM1306, RM157)를 이용하여 판별하였다. 판별용으로 이용된 SSR 마커 7개의 총 대립인자 수는 130개였으며, 대립인자 수의 범위는 10 ~ 32개이었고, 평균 대립인자 수는 18.57이었다. PIC 값은 0.679 (HsSSR01-52) ~ 0.895 (RM333)의 범위이었으며, 평균 PIC 값은 0.774이었다. SSR 마커 7개의 조합으로 6단계의 판별을 통해 총 243개 품종 중 243개 모든 품종의 구별이 가능하였다. 이와 같은 결과, 본 연구에 이용된 SSR 7개 마커는 한국 벼 품종의 판별과 순도유지에 매우 효율적으로 이용할 수 있을 것으로 여겨진다.

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