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http://dx.doi.org/10.5010/JPB.2017.44.3.243

Discrimination of Korean rice varieties as revealed by DNA profiling and its relationship with genetic diversity  

Kim, Me-Sun (Department of Crop Science, Chungbuk National University)
Song, Jae-Young (Department of Crop Science, Chungbuk National University)
Kang, Kwon-Kyoo (Department of Horticultural Life Science, Hankyong National University)
Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 243-263 More about this Journal
Abstract
This study is to establish the varietal discrimination based on DNA profiling of different varieties of rice. We examined the genetic distance among Korean rice varieties using allele frequencies and a genetic diversity analysis with Simple Sequence Repeats (SSRs) markers. The analysis of the genetic diversity and genetic relationships of 243 Korean rice varieties was varied out using 20 SSRs markers. A total of 268 alleles were detected, ranging from 6 to 32, with an average of 13.45 alleles per locus, and and average of gene diversity (GD) of 0.556. Seven SSR markers were selected as key markers for discrimination among the Korean rice varieties. Concerning the results, 243 varieties (100%) were discriminated among by using acrylamide gel and fragment analyzer-based markers. In conclusion, this study provides useful basic data that can be utilized concerning Korean rice varieties breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing the diversity of Korean rice varieties.
Keywords
Discrimination; Korean rice variety; Genetic diversity; Simple sequence repeats (SSRs);
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Times Cited By KSCI : 7  (Citation Analysis)
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1 He C, Poysa V, Yu K (2003) Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars. Theoretical and Applied Genetics 106(2):363-373   DOI
2 Hong JH, Kwon YS, Choi KJ (2013a) Analysis of genetic relationship of apple varieties using microsatellite markers. Journal of Life Science 23(6):721-727   DOI
3 Hong JK, Kwon YS, Choi KJ (2013b) Identification of rose (Rosa X hybrida) varieties using simple sequence repeat (SSR) markers. Korean Journal of Breeding Science 45(2):96-103   DOI
4 Hwang JH, Kim HJ, Chae Y, Choi HS, Kim MK, Park YH (2012) Evaluation of germplasm and development of SSR markers for Marker-assisted Backcross in Tomato. Korean Journal of Horticultural Science and Technology 30(5):557-567   DOI
5 Hwang TY, Nakamoto Y, Kono I, Enoki H, Funatsuki H, Kitamura K, Ishimoto M (2008) Genetic diversity of cultivated and wild soybeans including Japanese elite cultivars as revealed by length polymorphism of SSR markers. Breeding Science 58(3):315-323   DOI
6 Hwang TY, Seo MJ, Lee SK, Park HM, Jeong KH, Lee YY, Kim SL, Yun HT, Lee JE, Kim DW, Jung GH, Kwon YU, Kim HS, Kim YH (2012) Discrimination of 110 Korean Soybean Cultivars by Sequence Tagged Sites (STS)-CAPS Markers. Korean Journal of Breeding Science 44(3):258-272
7 Jang SJ, Park SJ, Park KH, Song HL, Cho YG, Jong SK, Kang JH, Kim HS (2009) Genetic diversity and identification of Korean elite soybean cultivars including certified cultivars based on SSR markers. Korean Journal of Crop Science 54(2):231-240
8 Kwon YS, Choi KJ (2013) Construction of a DNA profile database for commercial cucumber (Cucumis sativus L.) cultivars using microsatellite marker. Korean Journal of Horticultural Science and Technology 31(3):344-351   DOI
9 Kim GJ, Song YH, Gi GY, Kim ST, Lee JH, Han TH (2011) Appliation of UPOV data for the analysis of genetic variation in rose cultivars. Korean Journal of Horticultural Science and Technology 29(3):240-246
10 Kwon YS, Park EK, Park CU, Bae KM, Yi Si, Cho IH (2006) Identification of rice variety using Simple Sequence Repeat (SSR) marker. Korean Society of Life Science 16(6):1001-1005
11 Kim SH, Jung JW, Moon JK, Woo SJ, Cho YG, Jong JK, Kim HS. (2006) Genetic diversity and relationship by SSR markers of Korean soybean cultivars. Korean Journal of Breeding Science 51(3):248-258
12 Sun MM, Choi KJ, Kim HS, Song BH, Woo SH, Lee CW, Jong SK, Cho YG (2009) Genetic diversity and discrimination of recently distributed Korean cultivars by SSR markers. Korean Journal of Breeding Science 41(2):115-125
13 Park SJ, Shin YH, Hwang SG, Jang CS (2011) Genetic diversity and relationships of 7 rice cultivars in Korea. Journal of Agricultural, Life and Environmental Sciences 23(2):49-54
14 Rural Development Administration (RDA) (2003) Standard methods for agricultural experimental experiments
15 Singh RK, Sharma RK, Singh AK, Singh VP, Singh NK, Tiwari SP, Mohapatra T (2004) Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice. Euphytica 135(2):135-143   DOI
16 Tian HL, Wang FG, Zhao JR, Yi HM, Wang L, Wang R, Tang Y, Song W (2015) Development of maizeSNP3072, a high-throughput compatible SNP array, for DNA fingerprinting identification of Chinese maize varieties. Molecular Breeding 35(6):136   DOI
17 Yoon UH, Kim CK, Lee GS, Hahn JH, Lee JH, Kim YK, Ji HS, Mun JH, Lee TH, Kim TH (2011) Structural and expression analysis of glutelin genes in Oryza sativa L. Journal of Plant Biotechnology 38(20):176-185   DOI
18 Gao L, Jia J, Kong X (2016) A SNP-based molecular barcode for characterization of common wheat. PLoS ONE 11(3): e0150947   DOI