• Title/Summary/Keyword: SOD-like

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Chemical Components and Physiological Activities of Bamboo (Phyllostachys bambusoides Starf) Extracts Prepared with Different Methods (추출방법에 따른 대나무(왕대) 추출물의 화학성분 및 생리활성)

  • Ju, In-Ok;Jung, Gi-Tai;Ryu, Jeong;Choi, Joung-Sik;Choi, Yeong-Geun
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.542-548
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    • 2005
  • Chemical components and physiological activities Bamboo (Phyllostachys bambusoides Starf) extracts obtained by burning, dry heating or extracting with water or 70% ethanol and were investigated. Contents of soluble solid and total phenolic compounds were highest in the ethanol extract. Contents of polyphenols such as catechin, chlorogenic acid, caffeic acid, 3-hydroxy benzoic acid and ferulic acid were determined. Free sugars consisted of galactose, glucose, fructose, and sucrose. Organic acids including citric, tartaric, malic, succinic, and acetic acid were present in the bamboo extracts. Antioxidant activities of dry heat and ethanol extracts were higher than those of BHA or ${\delta}-tocopherol$. Nitrite- scavenging effect of extracts ranged from 84.7 to 99.6% at pH 1.2 ana 3.0. Tyrosinase-inhibitory activity was higher in the water extract, and SOD-like and ACE-inhibitory activity were highest in tile dry kent extract. Antimicrobial activities of the bamboo extracts were strong against Bacillus subtilis, Escherichia coli O157, and Staphylococcus aureus.

Effects of the Fermentation Periods on the Qualities and Functionalities of the Fermentation Broth of Wild Vegetables (발효기간이 산야채 발효액의 품질과 기능성에 미치는 영향)

  • Kim, Na-Mi;Lee, Jong-Won;Do, Jae-Ho;Yang, Jae-Won
    • Korean Journal of Food Science and Technology
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    • v.35 no.2
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    • pp.272-279
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    • 2003
  • To determine optimal fermentation period of wild vegetables mixed with black sugar without microorganisms during plant extract fermentation food processing, changes in chemical components, quality characteristics of the fermented broth, and physiological functionality during fermentation period were investigated. pH and $^{\circ}Bx$ of fermented broths decreased gradually during fermentation period. Except persimmon leaf, viscosity of fermented broths of wild vegetables decreased after 3 months fermentation period. Amylase activity increased to $167{\sim}800%$ of its initial level after 6 months fermentation period, and invertase activity decreased by $60{\sim}170$ units after 1 month fermentation. No significant level of cellulase activity was observed. In the sensory evaluation test, inherent flavors and tastes of the wild vegetable decreased during the fermentation period, while those of others gradually increased. Overall acceptability was the highest after 3 months fermentation. Content of total phenolic compounds and electron-donating ability were highest after 3 to 4 months fermentation period, and decreased thereafter. Except Mugwort, tyrosinase inhibitory activity was found in all fermented broths. SOD-like activities were $23.0{\sim}25.1$ and $27.0{\sim}29.2%$ in fermentation broths of acacia flower and persimmon leaf, respectively, and were maintained throughout the fermentation period. Based on these results, fermentation period of 3 to 4 months was determined to be appropriate for plant extract fermentation food processing.

Resveratrol Extraction from Grape Fruit Stem and its Antioxidant Activity (포도 송이가지를 이용한 레스베라트롤의 추출 및 항산화 활성)

  • Cho, Cheol-Hee;Kim, So-Young;Yoo, Gui-Jae;Son, Min-Hee;Park, Keun-Hyoung;Lim, Byung-Lak;Kim, Dong-Chung;Chae, Hee-Jeong
    • Applied Biological Chemistry
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    • v.51 no.1
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    • pp.11-16
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    • 2008
  • The extraction conditions for resveratrol production from grape fruit stem, which is a by-product of grape processing, were optimized to develop high-functional grape-based products. Additionally, the bioefficacy of grape fruit stem extract (GFSE) as an antioxidant agent was evaluated. Resveratrol was extracted using various experimental conditions such as extractant type, extractant concentration, raw material-extractant ratio, extraction time and temperature, and the results were analyzed using a statistical program (SPSS). The resveratrol yield was the highest when 80% ethanol with a raw-material-extractant ratio of 1:10 (w/v) was used. In addition, the optimal temperature and time were selected as $60^{\circ}C$ and 90 min, respectively. When the antioxidant activity was analyzed and expressed as DPPH radical scavenging activity and SOD-like activity, the antioxidant activity of GFSE was higher than that of BHT, BHA and L-ascorbic acid. Finally, it was found that GFSE could be used as a raw material for the production of high antioxidant agents.

Antioxidative and Antimicrobial Activities of Pomegranate Seed Extracts (석류씨 추출물의 항산화 및 항균활성)

  • Koh Jong-Ho;Hwang Myeong-O;Moon Joo-Soo;Hwang Seong-Yun;Son Jong-Youn
    • Korean journal of food and cookery science
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    • v.21 no.2 s.86
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    • pp.171-179
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    • 2005
  • This study was investigated on antioxidative and antimicrobial activities of PSW(pomegranate seed water extract), PSE(pomegranate seed ethanol extract) and PSO(pomegranate seed oil). The extraction yields of PSW, PSE and PSO were 28.9, 13.0 and $4.9\%$, respectively. Total phenol contents of PSW, PSE and PSO were 47, 78 mg/g(dry basis) and 40 mg/g, respectively. Electron donating abilities of PSW, PSE and PSO at 1,000 ppm were 18.8, 28.5 and $9.7\%$, respectively. Antioxidative activities in Iinoleic acid substrates at 500 ppm were in order of PSE > $\alpha-tocopherol$ > PSW > PSO. Antioxidative activities in Iinoleic acid emulsion substrates at 200 ppm were in order of $\alpha-tocopherol$l > PSE > PSW > PSO. In antimicrobial activity, PSO showed growth inhibition effect against Micrococcus luteus, Salmonella enteritidis and PSW showed growth inhibition effect against Bacillus cereus, Escherichia coli. Whereas antimicrobial activity of PSE was not observed. The nitrite-scavenging abilities of PSW, PSE and PSO at 2,000 ppm were 27.5, 23.7 and $39.6\%$, respectively. And the SOD-like activities of PSW, PSE and PSO at 1,000 ppm were 15.9, 34.9 and $0.10\%$, respectively.

Isoflavone Contents, Antioxidative and Fibrinolytic Activities of Red Bean and Mung Bean (팥과 녹두의 이소플라빈 함량과 항산화 및 혈전용해 활성)

  • 오혜숙;김준호;이명희
    • Korean journal of food and cookery science
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    • v.19 no.3
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    • pp.263-270
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    • 2003
  • This study was conducted to investigate the isoflavone (daidzein and genistein) contents, and the antioxidative and fibrinolytic activities of red and mung beans. Daidzein was not found in either the red or mung beans. The genistein contents of the red and mung beans were 40.7 and 27.8 mg/kg, respectively. Both samples had very high electron donating abilities and SOD-like activities. Fibrinolytic activities were not detected in the crude mung bean extract, but fibrinolytic substances were purified or activated under various pH conditions and with heat treatments. With heat treatment at 100 $^{\circ}C$ for 10 min, the specific activity was increased 4.61 fold. This study revealed that, although red and mung beans were poor in isoflavone, they could be good sources for functional products due to their strong antioxidative activities and heat- and acid-resistant proteolytic abilities.

Flavonoid Content and Antioxidant Activities of Gardenia jasminoides Ellis Seed Extracts Produced by Ethyl Acetate, Distilled Water and Methanol (치자(Gardenia jasminoides Ellis) 씨 에틸 아세테이트, 물 및 메탄올 추출물의 플라보노이드 함량 및 항산화 활성)

  • Jin, Dong-Hyeok;Oh, Da-Young;Kang, Dong-Soo;Lee, Young-Geun;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.4
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    • pp.1094-1103
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    • 2017
  • The purpose of this study was to measure the bioactivity and antioxidant activity of seed from Gardenia jasminoides Ellis fructus (GJE). We determined tannin content ($1.517{\pm}0.003mg\;CE/g$, mg of catechin equivalents). The extraction yields of the GJE seed solvent were distilled water (DW) 36.61%, 70% methanol 30.10% and ethyl acetate (EA) 20.40%. The antioxidant activities of the extracts were significantly increased (0.2, 0.4, 0.6 mg/mL). Flavonoid contents (mg QE/g, mg of quercetin equivalents) were observed in the order of 70% methanol (0.799), DW (0.565) and EA (0.117). Antioxidant activities (DPPH, OH, ferrous ion-chelating capacity) were similar to the flavonoid content of each solvent. But ABTS scavenging activity and SOD like ability of DW extract were higher than 70% methanol extract. Comparing the yield and the antioxidant activity of each solvent of GJE seed, it seems to be preferable to use DW and 70% methanol solvent for extraction. As a result of this experiment, it has high antioxidant activity and physiological activity, which is expected to be highly valuable as a functional food and a natural antioxidant.

Antioxidant and Fibrinolytic Activities of Extracts from Soybean and Chungkukjang (Fermented Soybean Paste) (대두와 청국장 추출물의 항산화능과 혈전용해능)

  • Joo, Eun-Young;Park, Chan-Sung
    • Food Science and Preservation
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    • v.18 no.6
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    • pp.930-937
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    • 2011
  • To develop natural preservatives or functional health foods from soybeans (Baektae and Taekwang), the antioxidant and fibrinoytic activities of the water and 70% (v/v) ethanol extract of soybeans and fermented soybean paste (Chungkukjang) were examined. The polyphenol contents of water extracts from Baektae and Baektae Chungkukjang were 189.25 mg/100 g and 814.58 mg/100 g, respectively, whereas those from Taekwang and Taekwang Chungkukjang were 210.23 mg/100 g and 834.23 mg/100 g. The polyphenol contents of the water extracts from Chungkukjang was 4.0~4.3-fold higher than those from soybean. The electron-donating abilities (EDAs) and superoxide dismutase-(SOD)-like activities of the water and ethanol extracts from Chungkukjang were higher than those of the extracts from soybean. Both extracts were found to have had fibrinolytic activity, and the highest activity was present in the water extracts of Baektae Chungkukjang. The polyphenol contents and antioxidative and fibrinolytic activities of the extracts from Chungkukjang were higher than those of the other soybean extracts. These results indicate that Chungkukjang can be used as a material for health foods or natural antioxidants.

Optimization of Extraction Conditions for Mate (Ilex paraguarensis) Ethanolic Extracts (Mate (Ilex paraguarensis) 에탄올 추출물의 추출조건 최적화)

  • Yang, Su-Jin;Youn, Kwang-Sup;No, Hong-Kyoon;Lee, Shin-Ho;Hong, Joo-Heon
    • Food Science and Preservation
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    • v.18 no.3
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    • pp.319-327
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    • 2011
  • This study was conducted to monitor the quality characteristics of mate (Ilex paraguarensis) ethanolic extracts via the response surface methodology. In the extraction conditions that were based on the central composite design with variations in the ethanol concentration (0-100%), extraction temperature($35-95^{\circ}C$), and the ratio of the solvent to the sample (10~30 mL/g). The extraction yield and total polyphenol content improved with the increase in the ethanol concentration than in the extraction temperature. The caffeic acid content increased with the decrease in the solvent ratio. The coefficients of determinations ($R^2$) were 0.8842 (p<0.05), 0.8729 (p<0.05), and 0.9205 (p<0.05) in terms of the electron donating ability, nitrite scavenging effect (pH 3.0), and SOD-like ability, respectively. The estimated conditions for the maximized extraction, including in terms of the yield, total polyphenol content, caffeic acid content, and electron donating ability, were a 21-48% ethanol concentration, a $76.4^{\circ}C$ extraction temperature, and 10-14mL/g solvent-to-sample ratio.

Comparison of Antioxidant, Wrinkle Improvement, and Whitening Efficacies of Extracts from Pinus koraiensis Cone Scale Using Extraction Methods (추출방법에 따른 잣나무(Pinus koraiensis) 구과피 추출물의 항산화, 주름개선 및 미백 효능 비교)

  • Chae, Jungwoo;Kim, Jeongyou;Jo, Huiseon;Lee, Jin-young
    • Journal of Korean Society of Forest Science
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    • v.110 no.3
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    • pp.431-439
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    • 2021
  • The present study evaluated the antioxidant activity, skin wrinkle reduction, and whitening activity of Pinus koraiensiscone scale extracts made with three different solvents (ethanol, supercritical fluid, and a mixture of both). Total polyphenol content was 11.03 mg/g GAE in the supercritical fluid extractand 33.79 mg/g GAE in the 70% ethanol extract. Electron donating ability of 1,000 ㎍/mL extract was 13.60% in the supercritical fluid extract, 91.37% in 70% ethanol extract, and 71.62% in mixed solvent extract. SOD-like activities in 100 ㎍/mL extract using supercritical fluid, 70% ethanol, and mixed solvents were 16.49%, 21.84%, and 10.7%, respectively. The ABTS+ radical scavenging activities of 1,000 ㎍/mL extract were 38.19%, 80.23%, and 78.72%for supercritical fluid extract, 70% ethanol extract, and mixed solvent extract, respectively. Tyrosinase inhibitory activities in 1,000 ㎍/mL extract were 24.54%, 36.55%, and 15.23% for supercritical fluid extract, 70% ethanol extract, and mixed solvent extract, respectively. Elastase inhibitory activities in 1,000 ㎍/mL extract were 15.62%, 22.56%, and 26.64% for supercritical fluid extract, 70% ethanol extract, and mixed solvent extract, respectively. Skin astringent activity (81.23% with 5000 ㎍/mL extract) was only detected in the 70% ethanol extract. Supercritical fluid and mixed solvent extracts showed no such activity. Our analysis of Pinus koraiensiscone scale extracts show that the highest aggregate activity was found in the 70% ethanol extract, followed by mixed solvent and supercritical fluid extracts. Therefore, our results oppose the hypothesis stating supercritical extract has the highest total polyphenol content and antioxidant activity.

Antioxidant Effect of Anserine Extracted from Salmon (Oncorhynchus keta) (연어(Oncorhynchus keta) 추출 anserine의 항산화 효과)

  • Min, Hye-Ok;Song, Ho-Su
    • Journal of Food Hygiene and Safety
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    • v.34 no.4
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    • pp.396-403
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    • 2019
  • Ion-exchange chromatography and ultrafiltration were used to extract anserine from salmon (Oncorhynchus keta). The salmon anserine showed DPPH radical scavenging activity in the range of 7.30% to 31.05% in a dose-dependent manner. This reducing power of salmon anserine also increased as the concentration increased. Metal chelate activity, superoxide dismutase - like activity, and thiobarbituric acid reactive substances assay showed similar results. The anserine also suppressed the increment of the peroxide value and linoleic acid during storage periods. These results suggest that salmon anserine might be useful as a natural antioxidant in various foodstuffs.