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Antioxidative and Antimicrobial Activities of Pomegranate Seed Extracts  

Koh Jong-Ho (Hanilyanhhang Co. Ltd.)
Hwang Myeong-O (Hanilyanhhang Co. Ltd.)
Moon Joo-Soo (Institute of Food Industry and Biotechnology, Department of Food and Biotechnology, Hankyong National University)
Hwang Seong-Yun (Institute of Food Industry and Biotechnology, Department of Food and Biotechnology, Hankyong National University)
Son Jong-Youn (Institute of Food Industry and Biotechnology, Department of Food and Biotechnology, Hankyong National University)
Publication Information
Korean journal of food and cookery science / v.21, no.2, 2005 , pp. 171-179 More about this Journal
Abstract
This study was investigated on antioxidative and antimicrobial activities of PSW(pomegranate seed water extract), PSE(pomegranate seed ethanol extract) and PSO(pomegranate seed oil). The extraction yields of PSW, PSE and PSO were 28.9, 13.0 and $4.9\%$, respectively. Total phenol contents of PSW, PSE and PSO were 47, 78 mg/g(dry basis) and 40 mg/g, respectively. Electron donating abilities of PSW, PSE and PSO at 1,000 ppm were 18.8, 28.5 and $9.7\%$, respectively. Antioxidative activities in Iinoleic acid substrates at 500 ppm were in order of PSE > $\alpha-tocopherol$ > PSW > PSO. Antioxidative activities in Iinoleic acid emulsion substrates at 200 ppm were in order of $\alpha-tocopherol$l > PSE > PSW > PSO. In antimicrobial activity, PSO showed growth inhibition effect against Micrococcus luteus, Salmonella enteritidis and PSW showed growth inhibition effect against Bacillus cereus, Escherichia coli. Whereas antimicrobial activity of PSE was not observed. The nitrite-scavenging abilities of PSW, PSE and PSO at 2,000 ppm were 27.5, 23.7 and $39.6\%$, respectively. And the SOD-like activities of PSW, PSE and PSO at 1,000 ppm were 15.9, 34.9 and $0.10\%$, respectively.
Keywords
pomegranate seed; antioxidative activity; antimicrobial activity;
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  • Reference
1 AOAC. 1990. Official Methods of Analysis. 15th ed. Association of official analytical chemists. Washington, D.C., Cd 8-35
2 Aviram M, Dornfeld L. 2001. Pomegranate juice consumption inhibits serum angiotensin converting enzyme activity and reduces systolic blood pressure. Atherosclerosis, 158(1) : 195-198   DOI   ScienceOn
3 Gray JI, Dugan JLR 1975. Inhibition of N-nitrosamine formation in model food system. J. Food Sci., 40(4) : 981-985   DOI
4 Choi OK, Chung KC, Cho GS, Hwang MO, Yoo YS. 2002. Proximate compositions and Selected Phytoestrogens of Iranian Black Pomegranate Extract and Its Products. Korean J Food & Nutr.15 : 119-125
5 Nugteren DH, Christ-Hazelhof E.1987. Naturally occurring conjugated octadecatrienoic acids are strong inhibitors of prostaglandin biosynthesis. Prostaglandins, 33 403-417   DOI   ScienceOn
6 Takagi T. 1966. Stearic configurations of parinaric and punicic acid, J. Amer. Oil Chemist's Soc., 43 : 249   DOI
7 AOAC. 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists Washington, DC. U.S.A
8 Blois MS. 1958. Antioxidant determmation by the use of a stable free radical. Nature 26 : 1199-1200
9 JOCS. 1984. Standard methods for the analysis of fats, oils and related materials. Japan Oil Chemists' Society 2.4.12-71
10 Teresa-Satue M, Huang SW, Frankel EN. 1995. Effect of natural antioxidants in virgin olive oil on oxidative stability of refined, bleached and deodorized oilve oil. J Am. Oil Chem. Soc., 72(4) : 1131-1137   DOI   ScienceOn
11 Husain SR, Cillard J, Cullard P. 1987. Hydroxyl radical scavenging activity of flavonoids, Phytochem, 26 : 2489-2491   DOI   ScienceOn
12 Hopkins CY, Chrisholm MJ. 1968. Survey of the conjugated fatty acids of seed oils, J. Amer. Oil Chemist's Soc., 45 178   DOI
13 Kim MS, Lee DC, Hong JE, ChagnKS, Cho HY, Kwon YK, Kim HY. 2000. Antimicrobial effects of ethanol extracts from Korean and Indonesian plants. Korean J. Food Sci Technol., 32(4) : 949-958
14 Junko MO, Yoko OH, Hideyuki YH, Hioryuki Y. 2004. Pomegranate extract improves a depressive state and bone properties in menopausal syndrome model ovariectomized mice. Journal of Ethnopharmacology. 92(1):93-101   DOI   ScienceOn
15 Takahara U. 1985. Inhibition of lipoxygenase-dependent lipid peroxidation by quercetin. Phytochemistry, 24 : 1443-1446   DOI   ScienceOn
16 Poyrazoglu E. Gokmen V, Atrik, N. 2002. Organic acids and phenolic compounds in pomegranates (Punicca granatum L.) grown in Turkey. Journal of Food Composition and Analysis, 15(5) : 567-575
17 Suzuki R, Noguchi R, Ota T, Abe M, Miyashita K, Kawada T. 2001. Cytotoxic effect of conjugated trienoic fatty acids on mouse tumor and human monocycytic leukemia cells. Lipids, 36 477-482   DOI   ScienceOn
18 생약학교재편찬위원회공저. 2000. 생약학. 동명사. pp. 261-262
19 Longtin, R. 2003. The pomegranate nature, s power fruit? . J. Natl. Cancer Inst., 95 346-348   DOI   ScienceOn
20 Melgarejo P, Salazar DM, Amoros A. 1995. Total Lipids Content and Fatty Acid Composition of Seed Oils from Six Pomegranate Cultivars. Journal of the Science of Food and Agriculture, 69(2) 253-256   DOI   ScienceOn
21 Leaf CD, Vecchio AJ, Roe DA, Hotchkiss JH. 1987. Influence of ascobic acid dose on N-nitrosoproline fomtation in humans. Cacrinogenesis, 8 791-795   DOI   ScienceOn
22 Lee GD, Chang HG, Kim HK. 1997. Antioxidative and Nitrite-scavenging Activities of Edbile Mushrooms. Korean J Food Sci Technol. 29 : 432-436
23 Gray JI, Dugan JLR. 1975. Inibhition of N-nitrosantine formation in model food systems. J. Food Sci., 40 :981-984   DOI
24 Goldfien A, Monroe SE. 1997. In Basic & clinical endocrin-ology, 5th Ed.(Greenspan, F.S. and Strewler, G.J. eds) Appleton Lange Company, London, 474-477
25 AOAC. 1984. Official Methods of Analysis. 14th ed. Method 9.110. Association of Official Analytical Communities Arlington, VA. U.S.A
26 Kato H, Lee IE, Chyune NY, Kim SB, Hayase F. 1987. Inhibitory of nitrosamine formation by nondilyzable melanoidins. Agric. BioI. Chem 51(1) 1333-1338
27 Schubert SY, Lansky EP, Neeman I. 1999. Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J Ethnopharmacol, 66 : 11-17   DOI   ScienceOn
28 lio M., Moriyanm A, Matsumoto Y, Takai N, Fukumoto, M. 1985. Inhibition of xanthine oxidase by flavonoids. Agric. BioI. Chem., 49 : 2173-2182   DOI
29 한국생약학교수협의회저. 2000. 본초학. 아카데미서적. pp. 850-852
30 한국 약용식물학 연구회저. 2001. 종합 약용식물학. 학창사. pp. 240-241