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http://dx.doi.org/10.12925/jkocs.2017.34.4.1093

Flavonoid Content and Antioxidant Activities of Gardenia jasminoides Ellis Seed Extracts Produced by Ethyl Acetate, Distilled Water and Methanol  

Jin, Dong-Hyeok (Department of Food Science and Technology, Pusan National University)
Oh, Da-Young (Department of Food Science and Technology, Pusan National University)
Kang, Dong-Soo (Department of Marine Bio Food Science, Chonnam National University)
Lee, Young-Geun (Department of Food Science and Technology, Pusan National University)
Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
Publication Information
Journal of the Korean Applied Science and Technology / v.34, no.4, 2017 , pp. 1094-1103 More about this Journal
Abstract
The purpose of this study was to measure the bioactivity and antioxidant activity of seed from Gardenia jasminoides Ellis fructus (GJE). We determined tannin content ($1.517{\pm}0.003mg\;CE/g$, mg of catechin equivalents). The extraction yields of the GJE seed solvent were distilled water (DW) 36.61%, 70% methanol 30.10% and ethyl acetate (EA) 20.40%. The antioxidant activities of the extracts were significantly increased (0.2, 0.4, 0.6 mg/mL). Flavonoid contents (mg QE/g, mg of quercetin equivalents) were observed in the order of 70% methanol (0.799), DW (0.565) and EA (0.117). Antioxidant activities (DPPH, OH, ferrous ion-chelating capacity) were similar to the flavonoid content of each solvent. But ABTS scavenging activity and SOD like ability of DW extract were higher than 70% methanol extract. Comparing the yield and the antioxidant activity of each solvent of GJE seed, it seems to be preferable to use DW and 70% methanol solvent for extraction. As a result of this experiment, it has high antioxidant activity and physiological activity, which is expected to be highly valuable as a functional food and a natural antioxidant.
Keywords
Seed of Gardenia jasminoides Ellis fructus; Antioxidant activity; Tannin; Flavonoid; Metallic chelate;
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1 A. E. Hagerman, C. T. Robbins, Y. Weerasuriya, T. C. Wilson and C. Mc Arthur, "Tannin chemistry in relation to digestion", J. Range Manage., Vol.45, pp. 57-62, (1992).   DOI
2 R. B. Walker and J. D. Everette, "Comparative reaction rates of various antioxidants with ABTS radical cation", J. Agric. Food Chem., Vol.57, No.4 pp. 1156-1161, (2009).   DOI
3 J. M. McCord and I. Fridovich, "Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein)", J. Biol. Chem., Vol.244, No.22 pp. 6049-6055, (1969).
4 S. Marklund and G. Marklund, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", European J. Biochem., Vol.47, No.3 pp. 469-474, (1974).   DOI
5 S. Dudonne, X. Vitrac, P. Coutiere, M. Woillez and J. M. Merillon, "Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays", J. Agric. Food Chem., Vol.57, No.5 pp. 1768-1774, (2009).   DOI
6 H. S. Kim, M. A. Kim and S. H. Jang, "Influences of Korean Haw (Crataegus pinnatifida BUNGE) on Lipid Concentration in Hypercholesterolemia", J. Environ. Sci. Int., Vol.23, No.5 pp. 793-800, (2014).   DOI
7 L. Mira, M. Tereza Fernandez, M. Santos, R. Rocha, M. Helena Florencio and K. R. Jennings, "Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity", Free Radical Res., Vol.36, No.11 pp. 1199-1208, (2002).   DOI
8 W. Droge, "Free radicals in the physiological control of cell function", Physiol. Rev., Vol.82, No.1 pp. 47-95, (2002).   DOI
9 D. Bagchi, A. Garg, R. L. Krohn, M. Bagchi, M. X. Tran and S. J. Stohs, "Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro", Res. Commun. Mol. Pathol. Pharmacol., Vol.95, No.2 pp. 179-189, (1997).
10 N. J. Miller, C. Rice-Evans, M. J. Davies, V. Gopinathan and A. Milner, "A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates", Clin. Sci. (London, England: 1979), Vol.84, No.4 pp. 407-412, (1993).   DOI
11 H. Aebi, "[13] Catalase in vitro", Methods enzymol, Vol.105, pp. 121-126, (1984).
12 M. Serafini and D. Del Rio, "Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool?", Redox Rep., Vol.9, No.3 pp. 145-152, (2013).   DOI
13 H. O. Edeoga, D. E. Okwu and B. O. Mbaebie, "Phytochemical constituents of some Nigerian medicinal plants", African J. Biotechnol., Vol.4, No.7 pp. 685-688, (2005).   DOI
14 S. T. Koo, M. S. Cho, S. S. Park, Y. T. Kim, K. J. Park, K. S. Kim and I. C. Sohn, "Effect of frutus gardeniae herbal acupuncture on the rat model of ankle sprain pain", Korean J. Acupunct., Vol.22, No.2 pp. 57-74, (2005).
15 A. Floegel, D. O. Kim, S. J. Chung, S. I. Koo and O. K. Chun, "Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods", J. Food Compos. Anal., Vol.24, No.7 pp. 1043-1048, (2011).   DOI
16 M. Yamauchi, K. Tsuruma, S. Imai, T. Nakanishi, N. Umigai, M. Shimazawa and H. Hara, "Crocetin prevents retinal degeneration induced by oxidative and endoplasmic reticulum stresses via inhibition of caspase activity", European J. Pharmacol., Vol.650, No.1 pp. 110-119, (2011).   DOI
17 D. H. Jin, H. S. Kim, J. H. Seong and H. S. Chung, "Comparison of total phenol, flavonoid contents, and antioxidant activities of Orostachs japonicus A. Berger extracts", J. Environ. Sci. Int., Vol.25, No.5 pp. 695-703, (2016).   DOI
18 P. Schofield, D. M. Mbugua and A. N. Pell, "Analysis of condensed tannins: a review, Anim", Feed Sci. Technol., Vol.91, No.1 pp. 21-40, (2001).   DOI
19 A. Meda, C. E. Lamien, M. Romito, J. Millogo and O. G. Nacoulma, "Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity", Food Chemistry, Vol.91, No.3 pp. 571-577, (2005).   DOI
20 M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature, Vol.181, No.3 pp. 1199-1200, (1958).   DOI
21 J. M. McCord and I. Fridovich, "Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein)", J. Biol. Chem., Vol.244, No.22 pp. 6049-6055, (1969).
22 X. Li, "Solvent effects and improvements in the deoxyribose degradation assay for hydroxyl radical-scavenging", Food Chem., Vol.141, No.3 pp. 2083-2088, (2013).   DOI
23 S. Robu, A. Aprotosoaie, A. Miron, O. Cioanca, U. Stanescu and M. Hancianu, "In vitro antioxidant activity of ethanolic extracts from some Lavandula species cultivated in Romania", Farmacia, Vol.60, No.3 (2012).
24 K. E. Heim, A. R. Tagliaferro and D. J. Bobilya, "Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships", J. Nutr. Biochem., Vol.13, No.10 pp. 572-584, (2002).   DOI
25 M. Heinonen, "Antioxidant activity and antimicrobial effect of berry phenolics-a Finnish perspective", Mol. Nutr. Food Res., Vol.51, No.6 pp. 684-691, (2007).   DOI
26 J. H. Lee and S. R. Lee, "Analysis of phenolic substances content in Korean plant foods", Korean J. Food Sci. Technol., Vol.26, No.3 pp. 310-316, (1994).
27 K. A. Steinmetz and J. K. Potter, "Vegetables, fruit, and cancer. I. Epidemiology", Cancer Causes Control, Vol.2, No.5 pp. 325-357, (1991).   DOI
28 Y. E. Chu, C. L. Chang and H. F. Hsu, "Flavonoid content of several vegetables and their antioxidant activity", J. Sci. Food Agric., Vol.80, No.5 pp. 561-566, (2000).   DOI
29 D. Villano, M. S. Fernandez-Pachon, M. L. Moya, A. M. Troncoso and M. C. Garcia-Parrilla, "Radical scavenging ability of polyphenolic compounds towards DPPH free radical", Talanta, Vol.71, No.1 pp. 230-235, (2007).   DOI
30 V. Vaithiyanathan and S. Mirunalini, "Assessment of anticancer activity: A comparison of dose-response effect of ethyl acetate and methanolic extracts of Pergularia daemia (Forsk)", Oral Sci. Int., Vol.13, No.1 pp. 24-31, (2016).   DOI
31 B. S. Wolfenden and R. L. Willson, "Radical-cations as reference chromogens in kinetic studies of ono-electron transfer reactions: pulse radiolysis studies of 2, 2' -azinobis-(3-ethylbenzthiazoline-6-sulpho nate)", J. Chem. Soc, Perkin Trans., Vol.2, No.7 pp. 805-812, (1982).