• Title/Summary/Keyword: SID

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Construction of New P4-Derived Vector Plasmid Containing Tetracyclin Resistance Marker for the Bacteriophage P2-P4 System (박테리오파아지 P2-P4 시스템을 위한 tetracyclin resistance marker 함유 P4 유도체 벡터 플라스미드 조성)

  • Kim, Kyoung-Jin
    • Korean Journal of Microbiology
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    • v.39 no.2
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    • pp.118-122
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    • 2003
  • To develop vector plasmid for the bacteriophage P2-P4 system which is a useful experimental tool for the study of viral capsid assembly, we constructed a new P4-derived vector plasmid starting from P4 ash8 sid71 With recombinant DNA technology, a portion of P4 genome was deleted and tetracyclin resistance gene (terR) was introduced into P4 genome to give P4 selectivity. Resulting P4 ash8(sid71) terR was 12.09 kb long and could be converted to a viable bacteriophage with P2 infection. The burst size of induced bacteriophage form of P4 ash8(sid71) terR was determined. The CsCl buoyant equilibrium density gradient experiment of new P4 derivative suggested the upper limit of packaging capacity in P2-size head.

The Study on influence of test factors for WorldSID injury through AE-MDB side crash test (AE-MDB시험 시 인체모형 상해치에 대한 시험 인자 영향성 연구)

  • Sun, Hongyul;Han, Pyokyong;Oh, Eunkyung;Yoon, Ilsung
    • Journal of Auto-vehicle Safety Association
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    • v.7 no.1
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    • pp.7-12
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    • 2015
  • NCAP(New Car Assessment Program) makes vehicle manufacturer improve safety performance through free competition and customers guarantee vehicle selection by providing information of vehicle safety. That's why it is important not only to meet the regulation, but also to cope with NCAP. EuroNCAP(European New Car Assessment Program) side tests have conducted by using Progressive MDB and Euro SID II in order to reproduce crash test between two vehicles over 10 years. However various researches report that Progressive MDB and Euro SID II do not reflect evolving vehicle design, impact performance and biofidelity of human. Therefore EuroNCAP has the plan to conduct AE-MDB side crash test using WorldSID which is more evolved from 2015 by replacing Progressive MDB and EuroSID II. Automobile manufacturers need to develop safety performance for new test closely. This paper is to find test set-up parameters which affect into dummy injury instead of restraint system and to research on its tendency. It is processed with mini and small car to know influence as changing vehicle size and also analyzed by DFSS(Design for six sigma) which is one of optimization tools. DFSS is vaildated by simulating CAE with L18 orthogonal array of 6 control factors adjustable as EuroNCAP requirement.

Amino acid digestibility in plant protein sources fed to growing pigs

  • Son, Ah Reum;Park, Chan Sol;Park, Kyu Ree;Kim, Beob Gyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.11
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    • pp.1745-1752
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    • 2019
  • Objective: The objective was to determine standardized ileal digestibility (SID) of amino acids (AA) in 11 plant protein sources fed to growing pigs. Methods: Eleven feed ingredients used were sesame meal, two sources of soybean meal (SBM) produced in the Republic of Korea, a source of SBM produced in India, high-protein distillers dried grains (HPDDG), perilla meal, canola meal, copra meal, corn germ meal, palm kernel expeller, and tapioca distillers dried grains (TDDG). Experimental diets were prepared to contain each test ingredient as a sole source of AA, and a nitrogen-free diet was also prepared to estimate the basal ileal endogenous losses of AA. Twelve barrows surgically fitted with T-cannulas at the distal ileum with an initial body weight of 29.0 kg (standard deviation = 3.0) were individually housed in metabolism crates equipped with a feeder and a nipple drinker. A $12{\times}9$ incomplete Latin square design was employed with 12 experimental diets, 12 animals, and 9 periods. After a 5-d adaptation period, ileal digesta were collected on d 6 and 7 in each experimental period. Results: Values for apparent ileal digestibility of most indispensable AA in three sources of SBM were greater compared with other test ingredients except HPDDG and canola meal (p<0.05). Pigs fed diets containing SBM sources had also greater SID of most indispensable AA compared with those fed diets containing other test ingredients (p<0.05) except for HPDDG and canola meal. There was no difference in the apparent ileal digestibility and SID of AA among sources of SBM. The TDDG had the least value for the SID of methionine among test ingredients (p<0.05). Conclusion: The SID of most AA in SBM, HPDDG, and canola meal were greater than those in sesame meal, perilla meal, copra meal, and TDDG.

Assessment of Dose and Image Quality according to the Change of Distance from Source to Image Receptor and the Examination Posture during the Skull Lateral Radiography (두부 측 방향 방사선검사 시 선원 영상수용체간 거리와 검사 자세 변화가 선량과 영상품질에 미치는 영향)

  • Eun-Hye, Kim;Young-Cheol, Joo;Han-Yong, Kim;Dong-Hwan, Kim
    • Journal of radiological science and technology
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    • v.45 no.6
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    • pp.483-489
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    • 2022
  • This study proposes a new skull lateral examination, and provides an improved examination environment for patients and radiologists. The study was divided into three groups. One group was divided into the SID (source to image receptor distance) 110 ㎝ and 180 ㎝ in the skull lateral posture, the other group The other group was divided into an position in contact with the detector and an position without contact with the detector, and the other group was divided into male and female groups, considering that the difference in shoulder width between adult males and females would affect the dose and image quality. For dose evaluation, the ESD (entrance surface dose) was measured at the EAM (external auditory meatus), and the conditions were applied equally at 70 ㎸p, 200 ㎃, and 10 ㎃s. For image quality evaluation, SNR (signal to noise ratio) and CNR (contrast to noise ratio) were measured in frontal sinus, EAM, and sella turcica. As a result of ESD comparison, when sid 110 ㎝ to sid 180 ㎝ was changed among the three groups, ESD values decreased the most to 729.18±4.62 μ㏉ and 224.18±0.74 μ㏉ at 180 ㎝ (p<0.01). The values of SNR and CNR were statistically significant (p<0.01), but there was no qualitative difference. This shows that when the SID is 180 ㎝, it is possible to reduce the dose without lowering the image quality. So, It is suggested that the SID 180 ㎝ is used without contacting the detector when examining the skull lateral.

An Interpretation of QR Factorization in Subspace Identification

  • Takei, Yoshinori;Imai, Jun;Wada, Kiyoshi
    • 제어로봇시스템학회:학술대회논문집
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    • 1999.10a
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    • pp.121-124
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    • 1999
  • Subspace-based state space system identification (4SID) methods have been demonstrated to per-form well in a number of applications, but the properties of these have not been fully analyzed or understood yet. For applying the methods, no assumptions on structure of realization are needed and any coordinate transformation is allowed for the estimates. This is one reason why many kinds of properties expected for identification procedures have not been clarified yet. We illustrate, by using Schur complement, an interpretation of the R matrix yielded by the QR factorization in the 4SID procedure. The results in this paper can be useful for analysis of properties of parameters obtained by 4SID methods.

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Biomechanics of Sacroiliac Joint Dysfunction and Clinical Disease (엉치엉덩관절 통증과 임상 질환에 대한 생체역학)

  • Jeong, Seong-Gwan;Lee, Woo-Hyung;Kim, Kyung-Hwan
    • PNF and Movement
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    • v.8 no.1
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    • pp.41-50
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    • 2010
  • Pain originating from the sacroiliac joint(SIJ) has been associated with poor performance, yet specific diagnosis of sacroiliac dysfunction(SID) has been difficult to achieve. Clinical presentation of SID appears that pain and poor performance is responsive to local analgesia of periarticular structures with poorly defined pathology, and poor performance with bony pathological changes present as a result of chronic instability. Previous research indicates that physical examination cannot diagnose SIJ pathology. Earlier studies have not reported sensitivities and specificities of composites of provocation tests known to have acceptable inter-examiner reliability. Tests based on mechanics as manual provocation for SIJ pain have formed the basis of tests used to diagnose SIJ dysfunction. In this review summary, the purpose of this study was to describe the sacroiliac tests with a model of examination, diagnosis, and management of SID. Further research is warranted to determine whether SIJ tests is reliable means of evaluating innominate impairments.

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Single-Image Dehazing based on Scene Brightness for Perspective Preservation

  • Young-Su Chung;Nam-Ho Kim
    • Journal of information and communication convergence engineering
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    • v.22 no.1
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    • pp.70-79
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    • 2024
  • Bad weather conditions such as haze lead to a significant lack of visibility in images, which can affect the functioning and reliability of image processing systems. Accordingly, various single-image dehazing (SID) methods have recently been proposed. Existing SID methods have introduced effective visibility improvement algorithms, but they do not reflect the image's perspective, and thus have limitations that distort the sky area and nearby objects. This study proposes a new SID method that reflects the sense of space by defining the correlation between image brightness and haze. The proposed method defines the haze intensity by calculating the airlight brightness deviation and sets the weight factor of the depth map by classifying images based on the defined haze intensity into images with a large sense of space, images with high intensity, and general images. Consequently, it emphasizes the contrast of nearby images where haze is present and naturally smooths the sky region to preserve the image's perspective.

The Average Glandular Dose in Mammography and Quality Control of the Equipment Status (유방촬영검사에서 평균유선선량과 장치의 품질관리 실태)

  • Jung, Hong-Ryang;Hwang, Su-Lyun;Ha, Bon-Cheol
    • Journal of the Korean Society of Radiology
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    • v.5 no.3
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    • pp.111-120
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    • 2011
  • A purpose of study is to develop optimization and radiation dose exposure reference level by measuring actual radiation dose in condition of quality control of mammography equipment for 39 clinics. The result were as follows. First, we measured T-test separating radiology from general clinic. According to the test, mAs was measured at average 78.58 mAs; radiology at 80.16 mAs and general clinic at 77.22 mAs. And, kerma rate was measured at average 7.71 mGy/mR; radiology at 8.94 mGy/mR and general clinic at 6.66 mGy/mR. HVL was measured at average 0.42 mmAl; radiology at 0.40 mmAl and general clinic at 0.43 mmAl. Average glandular dose was measured at average 1.14 mGy; radiology at 1.09 mGy and general clinic at 1.19 mGy. Second, we measured value of mAs, HVL, processing method and so on dividing two groups. And, we compared and analyzed average value measured using T-test. As a result, there was significance level in SID(P<0.05). There was significance level in mAs(P<0.05). Because processor was measured at 1.00 mGy and CR at 1.17 mGy according to the processing method of radiology. Third, according to the correlation analysis, radiology had significance level between average glandular dose and mAs and general clinic had significance level between average glandular dose and SID(P<0.05). Forth, as a result of regression analysis, mAs affected 22.7%t of average glandular dose and SID affected 21.7% of average glandular dose, which had significance level(P<0.05). And, mAs affected 29.0% of average glandular dose in radiology and SID affected 29.1% of average glandular dose in general clinic, which was most influential.

Genetic Monitoring of Plant Growth Promoting Rhizobacterium (PGPR), Bacillus subtilis AH18 using Multiplex PCR in Field Soil (Multiplex PCR을 이용한 생물방제균 Bacillus subtilis AH18의 토양내 Genetic Monitoring)

  • Woo, Sang-Min;Lim, Jong-Hui;Jeong, Hee-Young;Kim, Sang-Dal
    • Microbiology and Biotechnology Letters
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    • v.37 no.1
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    • pp.1-9
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    • 2009
  • The genetic monitoring method was developed for the rapid detection of the PGPR and biocontrol agent, B. subtilis AH18 in red-pepper field soil by multiplex PCR using sid, aec and cel gene primers. The monitoring of B. subtilis AH18 in the soil was carried by amplified a 2,3-dihydro-2,3-dihydroxy benzoate dehydrogenase [EC: 1. 3. 1. 28]gene (sid - 794 bp : EF408238) which is a key enzyme of siderophore synthesis, an auxin efflux carrier gene (aec - 1,052 bp : EF408239) and a cellulase gene (cel - 1,582 bp : EF070194). The natural un sterilized soil was inoculated with B. subtilis AH18 to determine the sensitivity ($1.8\times10^5$ cfu/g) of multiplex PCR for the rapid dectection and then the strain was monitored successfully in rhizosphere or non-rhizosphere soil of red-pepper cultural soil. At 3 weeks after the treatment, density of the strain was monitored more abundantly in rhizosphere soil.

Construction and characterization of the bacteriophage P4 derivatives whose genome size suitable for packaging into a P2sir3-sized head (P2 sir3-sized head에 packaging 되기 용이한 크기의 박테리오파지 P4 유도체 조성과 정성 연구)

  • Kim, Kyoung-Jin
    • Korean Journal of Microbiology
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    • v.51 no.1
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    • pp.1-6
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    • 2015
  • The term "P2 sir-associated helper inefficiency" has been used to define the inefficient helper capability of P2 sir mutants for their satellite bacteriophage P4. The aim of this study was to investigate the factors overcoming P2 sir-associated helper inefficiency. At first, we verified whether the P2 cos region containing P4 sid71 cosP2 could overcome P2 sir-associated helper inefficiency with P2 sir3. The result was that P4 sid71 cosP2 could not overcome P2 sir-associated helper inefficiency with P2 sir3. Instead of cos region of P2, the size of the DNA packaged into a $P2_{sir}$-sized head seems to be important for overcoming P2 sir-associated helper inefficiency. In the present work, three kinds of P4 derivatives with packaged DNA sizes between those of P4 ost1 and P4 ost2, were constructed through DNA manipulation. In one P4 derivative, P4 sid71 delRI::apr, the size of the packaged DNA was identified with a CsCl buoyant equilibrium density gradient experiment. According to the burst sizes of the P4 derivatives, they could overcome P2 sir3-associated helper inefficiency. The size of the P4 derivative DNA suitable for packaging into a $P2_{sir3}$-sized head was 28-29 kb.