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Construction of New P4-Derived Vector Plasmid Containing Tetracyclin Resistance Marker for the Bacteriophage P2-P4 System  

Kim, Kyoung-Jin (Department of Applied Molecular Biology, Division of Applied Biology, Sunmoon University)
Publication Information
Korean Journal of Microbiology / v.39, no.2, 2003 , pp. 118-122 More about this Journal
Abstract
To develop vector plasmid for the bacteriophage P2-P4 system which is a useful experimental tool for the study of viral capsid assembly, we constructed a new P4-derived vector plasmid starting from P4 ash8 sid71 With recombinant DNA technology, a portion of P4 genome was deleted and tetracyclin resistance gene (terR) was introduced into P4 genome to give P4 selectivity. Resulting P4 ash8(sid71) terR was 12.09 kb long and could be converted to a viable bacteriophage with P2 infection. The burst size of induced bacteriophage form of P4 ash8(sid71) terR was determined. The CsCl buoyant equilibrium density gradient experiment of new P4 derivative suggested the upper limit of packaging capacity in P2-size head.
Keywords
bacteriophage P2-P4; Escherichia coli; packaging; plasmid;
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  • Reference
1 Determination of capsid size by satellite bacteriophage P4 /
[ Shore,D.;G.Deho;J.Tsipis;R.Goldstein ] / Proc. Natl. Acad. Sci. USA   DOI   ScienceOn
2 The capsid size-determining protein Sid forms an external scaffold on phage P4 procapsids /
[ Marvik,O.J.;T.Dokland;R.Nokling;E.Jacobson;T.Larsen;B.H.Lindqvist ] / J. Mol. Biol.   DOI   ScienceOn
3 Nonessential region of bacteriophage P4: DNA sequence, transcription, gene products and functions /
[ Ghisotti,D.;S.Finkel;C.Halling;G.Deho;G.Sironi;R.Calendar ] / J. Virol.
4 Capsid size datermination in the P2-P4 bacteriophage system: suppression of sir mutations in P2's capsid gene N by supersid mutations in P4's external scaffold gene sid /
[ Kim,K.J.;M.G.Sunshine;B.H.Lindqvist;E.W.Six ] / Virology   DOI   ScienceOn
5 Bacteriophage P2: physical, genetic and restriction map /
[ Linderoth,N.A.;G.Christie;E.Haggard Ljungquist;S.J.O'Brien(ed.) ] / Genetic map(6th ed.)
6 Complete nucleotide sequence of the E. coli plasmid pBR322 /
[ Sutcliffe,J.G. ] / Cold Spring Harbor Symposium   DOI   ScienceOn
7 Inheritance of prophage P2 in bacterial crosses /
[ Bertani,G.;E.W.Six ] / Virology   DOI   ScienceOn
8 /
[ Sambrook,J.;D.W.Russell ] / Molecular Cloning: a laboratory manual(3rd ed.)
9 Improved M13 phage cloning vectors and host strain: nucleotide sequences of the M13mp18 and pUC19 vectors /
[ Yanisch Perron,C.;C.J.Viera;J.Messing ] / Gene   DOI
10 박테리오파아지 P2-P4 시스템을 위한 벡터 플라스미드 개발과 안정성 /
[ 김경진 ] / 미생물학회지
11 Mutational analysis of the bacteriophage P4 capsid-size-determining gene /
[ Nilssen,O.;E.W.Six;M.G.Sunshine;B.H.Lindqvist ] / Virology   DOI   ScienceOn
12 Morphoietic switch mutations of bacteriophage P2 /
[ Six,E.W.;M.G.Sunshine;J.Williams;E.Haggard Ljungquist;B.H.Lindqvist ] / Virology   DOI   ScienceOn
13 Growth abnormalities in Hfr derivatives of Escherichia coli strain /
[ Sasaki,I.;G.Bertani ] / C. J. Gen. Microbiol.   DOI   ScienceOn
14 Bacteriophage P4: A satellite virus depending on a helper such as prophage P2 /
[ Six,E.W.;C.A.C.Klug ] / Virology   DOI   ScienceOn
15 The P2-like phages and their parasite, P4 /
[ Bertani,L.E.;E.W.Six;R.Calendar(ed.) ] / The Bacteriophages