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Genetic Monitoring of Plant Growth Promoting Rhizobacterium (PGPR), Bacillus subtilis AH18 using Multiplex PCR in Field Soil  

Woo, Sang-Min (Department of Applied Microbiology, Yeungnam University)
Lim, Jong-Hui (Department of Applied Microbiology, Yeungnam University)
Jeong, Hee-Young (Department of Applied Microbiology, Yeungnam University)
Kim, Sang-Dal (Department of Applied Microbiology, Yeungnam University)
Publication Information
Microbiology and Biotechnology Letters / v.37, no.1, 2009 , pp. 1-9 More about this Journal
Abstract
The genetic monitoring method was developed for the rapid detection of the PGPR and biocontrol agent, B. subtilis AH18 in red-pepper field soil by multiplex PCR using sid, aec and cel gene primers. The monitoring of B. subtilis AH18 in the soil was carried by amplified a 2,3-dihydro-2,3-dihydroxy benzoate dehydrogenase [EC: 1. 3. 1. 28]gene (sid - 794 bp : EF408238) which is a key enzyme of siderophore synthesis, an auxin efflux carrier gene (aec - 1,052 bp : EF408239) and a cellulase gene (cel - 1,582 bp : EF070194). The natural un sterilized soil was inoculated with B. subtilis AH18 to determine the sensitivity ($1.8\times10^5$ cfu/g) of multiplex PCR for the rapid dectection and then the strain was monitored successfully in rhizosphere or non-rhizosphere soil of red-pepper cultural soil. At 3 weeks after the treatment, density of the strain was monitored more abundantly in rhizosphere soil.
Keywords
Genetic monitoring; multiplex PCR (polymerase chain reaction); Bacillus subtilis AH18; PGPR; biocontrol;
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