• Title/Summary/Keyword: S&T Culture

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Culturing Method and Dietary Value of Benthic Copepod, Tigriopus japonicus (부착성 요각류, Tigriopus japonicus의 배양방법 및 먹이효율)

  • 박흠기;허성범;김철원
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.261-269
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    • 1998
  • The harpacticoid copepod, Tigriopus japonicus is one of the important zooplankton as a live food the production of marine fish fry. This reserch was carried out to investigate the culturing method and dietary value of T. japonicus. The reproduction of this copepod was analysed in the culturing systems (tne opened and the closed systems) and the surface area of the substate plates for the mass culture. Survival rate of the mature female and the number of nauplii produced per female for two day was higher in the open culturing system than in the close system. However, the result of survival rate of the nauplius was reversed. Larger surface area of the culturing vessel accommodated higher density of this bentic copepod. In polyculture of T. japonicus and rotifer (B. plicatilis), growth of T. japonicus seems to be dependent on the rotifer density. But rotifer seems to be independent on the copepod. With regard to dietary value, this copepod is better than Artemia nauplius for the larvae of red sea bream and tiger puffer which feed by picking. However, it is inadequate for bottom fish larvae as flounder.

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Lactococcus lactis Culture Methods for the Enhanced Depression of Inducers in Atopic Diseases (아토피유발인자 억제효과를 증대하는 Lactococcus lactis의 배양방법)

  • Jo, Yu-Ran;Kang, Sang-Mo
    • Microbiology and Biotechnology Letters
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    • v.40 no.4
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    • pp.310-318
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    • 2012
  • We conducted a screening and checked the cultivation methods of lactic acid bacteria, which have anti-atopic dermatitis functions, by determining the lactic acid bacteria's immune enhancement by FACS, and antimicrobial activity against Staphylococcus aureus. The increase of Tcell CD4+/CD25+/foxp3+ was bigger in Lactobacillus plantarum than Lactococcus lactis subsp. lactis (Lc. lactis) and the antimicrobacterial activity against S. aureus was the opposite. The antimicrobial activity of Lb. plantarum culture with medium containing Lc. lactis culture broth was not enhanced, but the antimicrobial activity of Lc. lactis cultured in a medium containing Lb. plantarum culture broth was enhanced. As the optimal method caltivation of Lc. lactis in a medium containing 10% of heat-killed Lb. plantarum culture broth was chosen. By this method, the antibacterial activity of the pure Lc. lactis culture increased sharply at the end of the log phase, while a restraint effect on the growth of S. aureus increased 1.29 times.

Program Development of Tea Culture for Tourism Product (다문화 관광상품 프로그램개발에 관한 연구)

  • Jung, Young-Sook;Kim, In-Sook
    • Journal of Global Scholars of Marketing Science
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    • v.8
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    • pp.1-19
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    • 2001
  • To develop the traditional tea culture into tourism product, we will review the characteristics of traditional tea culture. Since our ancestor introduced tea, our tea culture has expressed the culture and spirit of ancestors' life and practiced the body and mind. Based on this facts, we want to build the program for applying.the tea culture into the characteristics of tourism products. The program for tea culture is following; reception; visiting the Korean Tea Museum consisting of reception room, tea-related document room, tea pottery room and korean traditional dress room; experiencing tea ceremony, tea traditional foods, natural dyeing, tea pottery making and tea etiquette; seeing visitors out. However, we didn't evaluate the satisfaction of tourists from Japan, China, America and Europe who participated in this program through the objective data. But they understood the excellency and creativity of Korean traditional culture through experiencing Korean invisible-visible culture. Therefore this study intends to develop the program for the attractive and differentiated culture tourism and build the competitive model of Korean culture tourism product.

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The Effectiveness of Jeju Island Mixed Reality Tourism Application: Focusing on the Moderating Role of Users' K-Culture Involvement

  • Yoo, Seungchul;Jung, Kwanghee;Nguyen, Vinh T.;Piscarac, Diana
    • International Journal of Advanced Culture Technology
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    • v.8 no.2
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    • pp.116-128
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    • 2020
  • In this study, we introduce a multi-modal mixed reality (MR) application for advertising the main touristic landmarks on Jeju Island, and evaluate its effectiveness based on the Technology Acceptance Model (TAM) with a focus on the user's involvement in Korean culture (K-Culture). Specifically, we deployed several stimuli to enable users to retrieve information, visualize a variety of heritage content such as text, images, videos, virtual artifacts, and immerse in a VR environment to experience the heritage locations. The results of the online survey with 436 participants showed that for consumers with low K-Culture involvement, Perceived Usefulness and Perceived Ease-of-Use did not affect their Intention to Visit the island. Still, it had a significant impact on their Intention to Use the application. On the other hand, for the group with high K-Culture involvement, Perceived Usefulness had no effect on the Intention to Use and Intention to Visit. However, Perceived Ease-of-Use had a significant impact on their Intention to Use the application and Intention to Visit Jeju Island. Therefore, when user involvement in K-Culture is high, convenience of use has played a significant role in sensory media usage and the intention to visit, regardless of the usefulness of the mixed reality content. In contrast, users with low K-Culture involvement primarily focused on the functional aspects of the application. These findings open the path for follow-up studies on K-Culture involvement and immersive media tourism marketing, which will benefit digital tourism marketing and Korea's tourism industry.

CD30-Mediated Regulation of Cell Adhesion Molecule Expression on Murine T Cells

  • Nam, Sang-Yun
    • IMMUNE NETWORK
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    • v.3 no.1
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    • pp.8-15
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    • 2003
  • Background: CD30 is a member of TNF receptor family and expressed on lymphocytes and other hematopoietic cells following activation as well as Hodgkin and Reed-Sternberg cells in Hodgkin's lymphoma. In this study, CD30-mediated regulation of cell adhesion molecule expression on normal activated mouse T cells was investigated. Methods: Mouse T cells were activated with anti-CD3 antibody for induction of CD30, which was cross-linked by immobilized anti-CD30 antibody. Results: High level of CD30 expression on T cells was observed on day 5, but only little on day 3 even under culture condition resulting in an identical T cell proliferation, indicating that CD30 expression requires a prolonged stimulation up to 5 days. Cross-linking of CD30 alone altered neither proliferation nor apoptosis of normal activated T cells. Instead, CD30 appeared to promote cell adherence to culture substrate, and considerably upregulated ICAM-1 and, to a lesser extent, ICAM-2 expression on activated T cells, whereas CD2 and CD18 (LFA-1) expression was not affected. None of cytokines known as main regulators of ICAM-1 expression on tissue cells (IL 4, $IFN{\gamma}$ and $IFN{\alpha}$) enhanced ICAM-1 expression in the absence of CD30 signals. On the other hand, addition of $NF-{\kappa}B$ inhibitor, PDTC (0.1 mM) completely abrogated the CD30-mediated upregulation of ICAM-1 expression, but not CD2 and ICAM-2 expression. Conclusion: This results support that CD30 upregulates ICAM-1 expression of T cell and such regulation is not mediated by higher cytokine production but $NF-{\kappa}B$ activation. Therefore, CD30 may play important roles in T-T or T-B cell interaction through regulation of ICAM-1, and -2 expression.

A Study on Consumer′s Concerns on Textile Fabrics in Children′s Clothings (유아복 섬유소재에 대한 소비자의 관심도 연구)

  • 김선경;이희선
    • The Research Journal of the Costume Culture
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    • v.5 no.1
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    • pp.137-150
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    • 1997
  • This study examined the effects of demographic characteristics (education, income level) on the relationships among knowledge of textile fibers, information searching behavior on textile fabrics, and information sources of textile materials in children's clothings. The subjects were 307 mothers with preschool children(4∼6years) in Seoul. Data were collected using a self-administered questionnaire and analyzed using T-test analysis of variance, corelations, and crosstabulation analysis. The results indicated that : (1) Educational levels of consumers had significant influences on knowledge of textile fibers, information searching behavior on textile fabric, and information sources of textile materials in children's clothings. (2) There were significant relationship between consumer's knowledge of textile fibers and information searching behavior on textile materials in children's clothings.

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Influencing Factors on Presenteeism of Clinical Nurses (임상간호사의 프리젠티즘에 영향을 미치는 요인)

  • Yeom, Eun Yi;Jeong, Gye Seon;Kim, Kyoung Ah
    • Korean Journal of Occupational Health Nursing
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    • v.24 no.4
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    • pp.302-312
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    • 2015
  • Purpose: This study was conducted to identify the influencing factors of job stress, self-efficacy, and organizational culture to presenteeism in clinical nurses. Methods: A descriptive correlation research design was used for the this study. The subjects were 245 nurses working at the hospital in S, A and C City, from August $1^{st}$ to October $30^{th}$ 2014. Data were analyzed using t-test, ANOVA, $Scheff{\acute{e}}^{\prime}s$ test, stepwise multiple regression by SPSS 21.0 program. Results: Job impairment indicated a significant positive relationship to job stress (p=.009), health problem (p<.001), and indicated significant negative relationship to self-efficacy (p<.001), innovative-oriented (p<.001), affiliative-oriented (p=.002), task-oriented (p=.026) in organizational culture. Significant factors of influencing to presenteeism were self-efficacy (p<.001, ${\beta}=-.363$), culture of task-oriented (p<.001, ${\beta}=-.248$) in organizational culture, and health problem (p=.002, ${\beta}=.187$). Conclusion: Self-efficacy was defined as a variable to influence on presenteeism, and developing of strategies and program to strengthen self-efficacy and task-oriented of organizational culture in nursing is helpful to reduce the presenteeism of clinical nurses.

The Opinion about 'Hanllyu' and the Domestic Apparel Product Buying Behavior of Foreigners Residing in Seoul (국내 거주 외국인의 한류(韓流)에 대한 견해와 국내 의류 제품 구매 행동)

  • Kim, Sung-Pil;Lee, Hong-Sup;Park, Eun-Hae;Hwang, Choon-Sup
    • The Research Journal of the Costume Culture
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    • v.15 no.4
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    • pp.580-592
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    • 2007
  • The purpose of this study is to analyse the foreigner's opinion about 'Hanllyu', and their buying behavior of clothes carried in Korean domestic market. The study was implemented through a normative-descriptive survey using self-administered questionnaires. The samples consisted of 75 foreigners residing in Korea. Data were analyzed by following statistical methods: frequency analysis, ${\chi}^2-test$, t-test, ANOVA, Scheffe's multiple range test. Results are as follows: There was no difference in the opinion about 'Hanllyu' between Chinese and Japanese. They regarded 'Hanllyu' as the phenomenon of the wave of Korean popular culture. Korean soap operas and movies were the main information sources on 'Hanllyu' stars for them. Significant differences were found in the behavior toward 'Hanllyu' stars according to sex and country, but there was no difference in the behavior according to their age. They mainly buy clothes in Dongdaemoon market and the main motivation of purchase were good design and price. The reason for dis-purchase was poor design. Respondents thought 'Hanllyu' fashion is gorgeous. There was significant difference in the behavior toward Korean culture according to sex.

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A Study on Street Fashion of Korean Teenager -Since the Latter Half of the `90s- (한국 청소년의 거리패션 분석 연구 -1990년대 후반을 중심으로-)

  • 김주영;김소영;양숙희
    • The Research Journal of the Costume Culture
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    • v.5 no.3
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    • pp.96-117
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    • 1997
  • The purpose of this study is focused on revealing teenager characteristics and mass culture in the end of the 20th century, and researching the general traits of teenager costume in the latter half of the \`90s and the typical style based on the analysis of the teenager culture. The result of this study are following as; The general traits of teenager costume are classified with sports-orientation, brand-orientation, and foreign street fashion-orientation. Typical styles are classified with sportive look, hiphop look, and funny look. Sportive look became the core of the street fashion developed with the street sports in the city, and presented the practical use with the fashionability mixing sportswear such as hightech snickers, sports character wear and items. Hiphop look, the genderless fashion, expresses teenager\`s free life style and the diversiied sensibility and deconstructs the border of gender, racism. Funny look accepts the burden of the millenium as a humor and presents katharsis by creating unexpected style. The contemporary costume of teenager deconstructs the fixed idea about mix and match, good taste and bad taste, gender, coordination suited with T.P.O and intends ‘open costume’ for 21st century.

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Inhibitory Effects of 14 Plants from Mongolia and Myanmar on Lipid Accumulation in 3T3-L1 and HepG2 Cells (몽골과 미얀마 식물 14종의 3T3-L1 및 HepG2 세포에서 지질 축적 억제효과)

  • Kim, SukJin;Kim, Gun-Hee
    • Journal of the Korean Society of Food Culture
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    • v.36 no.1
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    • pp.130-142
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    • 2021
  • This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.