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http://dx.doi.org/10.4014/kjmb.1205.05025

Lactococcus lactis Culture Methods for the Enhanced Depression of Inducers in Atopic Diseases  

Jo, Yu-Ran (Dept. of Microbial Engineering, Konkuk University)
Kang, Sang-Mo (Dept. of Microbial Engineering, Konkuk University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.4, 2012 , pp. 310-318 More about this Journal
Abstract
We conducted a screening and checked the cultivation methods of lactic acid bacteria, which have anti-atopic dermatitis functions, by determining the lactic acid bacteria's immune enhancement by FACS, and antimicrobial activity against Staphylococcus aureus. The increase of Tcell CD4+/CD25+/foxp3+ was bigger in Lactobacillus plantarum than Lactococcus lactis subsp. lactis (Lc. lactis) and the antimicrobacterial activity against S. aureus was the opposite. The antimicrobial activity of Lb. plantarum culture with medium containing Lc. lactis culture broth was not enhanced, but the antimicrobial activity of Lc. lactis cultured in a medium containing Lb. plantarum culture broth was enhanced. As the optimal method caltivation of Lc. lactis in a medium containing 10% of heat-killed Lb. plantarum culture broth was chosen. By this method, the antibacterial activity of the pure Lc. lactis culture increased sharply at the end of the log phase, while a restraint effect on the growth of S. aureus increased 1.29 times.
Keywords
Lactobacillus plantarum; Lactococcus lactis subsp. lactis; immune enhancement; antimicrobial activity; Staphylococcus aureus; heat-killed;
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1 Sakaguchi, S. 2005. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and non-self. Nat. Immunol. 6: 345-352.
2 Sakaguchi, S. and F. Powrie. 2007. Emerging challenges in regulatory T cell function and biology. Science 317: 627-629   DOI
3 Sakaguchi, S., N. Sakaguchi, M. Asano, M. Itoh, and M. Toda. 1995. Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J. Immunol. 155: 1151-1164.
4 Song, D. H. 2010. Anti-atopic effect in NC/Nga mouse by oxymatrine, matrine, trifolirhizin, kurarinone of Sophora flavescens Solander ex Ation. Konkuk university a doctoral dissertation.
5 Song, M. K., S. G. Woo, J. S. Jang, J. H. Kim, H. Y. Kim, S. G. Hong, B. W. Lee, M. H. Park, and K. S. Chung. 2003. Immunostimulating and and anti-cancer effects of Pediococcus pentosaceus EROM101 isolated from Korea. Kor. J. Microbiol. Biotechnol. 31: 355-361.
6 Leung, D. Y. 1999. Pathogenesis of atopic dermatitis. J. Allergy Clin. Immunol. 104: 99-108.   DOI   ScienceOn
7 Li, C., J. Bai, Z. Cai, and F. Ouyang. 2002. Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. J. Biotechnol. 93: 27-34.   DOI   ScienceOn
8 Li, N., W. M. Russell, M. Douglas-escobar, N. Hauser, M. Lopez, and J. Neu. 2009. Live and heat-killed Lactobacillus rhamnosus GG: effects on proinflammatory and anti-inflammatory cytokines/chemokines in gastrostomy-fed infant rats. Pediatr. Res. 66: 203-207.   DOI   ScienceOn
9 Matsui, K., A. Nishikawa, H. Suto, R. Tsuboi, and H. Ogawa. 2000. Comparative study of Staphylococcus aureus isolated from lesional and non-lesional skin of atopic dermatitis patients. Microbiol. Immunol. 44: 945-947.
10 Mosmann, T. R. and R. L. Coffman. 1989. Th1 and Th2 cells: diffferent patterns of lymphokine secretion lead to different functional properties. Annu.. Rev. Immunol. 7: 145-173   DOI   ScienceOn
11 Nascimento Maristelas, Moreno Izildinha, and Kuaye arnaldoy. 2008. Applicability of bacteriocin-producing Lactobacillus plantarum, Enterococcus faecium and Lactococcus lactis ssp. lactis as adjunct starter in Minas Frescal cheesemaking. J. Dairy technol. 61: .352-357.   DOI   ScienceOn
12 Okudaira, H., O. Hongo, T. Ogita, M. Haida, N. Yamauchi, and T. Miyamoto. 1983. Serum IgE and IgE antibody levels in patients with bronchial asthma, atopic dermatitis, eosinophilic granulomas of the soft tissue (Kimura's disease) and other diseases. Ann. Allergy. 50: 51-54.
13 Oumer, A., S. Garde, and P. Gaya. 2001. The effects of cultivating lactic starter cultures with bacteriocin-producinglactic acid bacteria. J. Food Protection 64: 81-86.
14 Pandiyan, P., L. Zheng, S. Ishihara, J. Reed, and M. J. Lenardo. 2007. CD4+CD25+Foxp3+ regulatory T cells induce cytokine deprivation-mediated apoptosis of effector CD4+ T cell. Nat. Immunol. 8: 1353-1362.   DOI   ScienceOn
15 Riley, M. A. 1998. Molecular mechanisms of bacteriocin evolution. Annu. Rev. Genet. 32: 255-278.   DOI   ScienceOn
16 Park, S. K., J. K. Han, and Y. H. Kim. 2008. Effect of Kami- Cheongsimyeonjatang on cytokine expression with GATA3 regulation in atopic dermatitis-like skin lesions and IgE hyperproduction induced in NC/Nga mice. Dept. of Pediatrics, College of Oriental Medicine, Daejeon University. 17: 167-183
17 Penna, T. C., A. F. Jozala, L. C. De Lencastre Novaes, A. Pessoa, and O. Cholewa. 2005. Production of nisin by Lactococcus lactis in media with skimmed milk. Appl. Biochem. Biotechnol. 121: 619-638.
18 Repa, A., C. Grangette, C. Daniel, R. Hochreiter, K. Hoffmann- Sommergruber, J. Thalhamer, D. Kraft, H. Breiteneder, A. Mercenier, and U. Wiedermann. 2003. Mucosal co-application of lactic acid bacteria and allergen induces counterregulatory immune responses in a murine model of birch pollen allergy. Vaccine 22: 87-95.   DOI   ScienceOn
19 Ring, J., D. Abeck, and K. Neuber. 1992. Atopic eczema: role of microorganisms on the skin surface. Allergy 47: 265-269   DOI   ScienceOn
20 Barefoot, S. F., Y. R. Chen, and T. A. Hughes. 1994. Identification and purification of a protein that induces production of the Lactobacillus acidophilus bacteriocin lactacin B. Appl. Environ. Microbiol. 60: 3522-3528.
21 Barnes, P. J. 2008. Immunology of asthma and chronic obstructive pulmonary disease. Nat. Immunology 8: 183-192   DOI   ScienceOn
22 Brunkow, M. E., E. W. Jeffery, K. A. Hjerrild, B. Paeper, L. B. Clark, S. A. Yasayko, J. E. Wilkinson, D. Galas, S. F. Ziegler, and F. Ramsdell. 2001. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scrufy mouse. Nat. Genet. 27: 68-73.
23 Dykes, G. A. and J. W. Hastings. 1997. Selection and fitness in bacteriocin-producing bacteria. Proc. Biol. Sci. 264: 683-687   DOI   ScienceOn
24 Chae, O. W., K. S. Shin, H. K. Chung, and T. B. Choe. 1998. Immunostimulation effects of mice fed with cell lysate of Lactobacillus plantarum isolated from kimchi. Kor. J. Biotechnol. Bioeng. 13: 424-430.
25 Cho, S. Y. 2007. Effects of tea extracts on the formation of biofilm and production of enterotoxins by Staphylococcus aureus. Chonnam national university a doctoral dissertation.
26 Chung, J. H., Y. S. Bae, Y. J. Kim, and J. H. Lee. 2010. Characteristics of bacteriocin produced by a Lactobacillus plantarum strain isolated from Kimchi. Kor. J. Microbiol. Biotechnol. 38: 481-485.
27 Elias, P. M. and M. Steinhoff. 2008. "Outside-to-inside" (and now back to "outside") pathogenic mechanisms in atopic dermatitis. J. Invest. Dermatol. 128: 1067-1070.   DOI   ScienceOn
28 Fiorentino, D. F., M. W. Bond, and T. R. Mosmann. 1989. Two types of mouse T helper cell IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J. Exp. Med. 170: 2081-2095.   DOI   ScienceOn
29 Fontenot, J. D. and A. Y. Rudensky. 2005. A well adapted regulatory contrivance: regulatory T cell development and the forkhead family transcription factor Foxp3. Nat. Immunol. 6: 331-337.   DOI   ScienceOn
30 Hayashi, A., M. Kimura, Y. Nakamura, and H. Yasui. 2009. Anti-atopic dermatitis effects and the mechanism of lactic acid bacteria isolated from Mongolian fermented milk. J. Dairy. Res. 76: 158-64.   DOI   ScienceOn
31 Hirose, Y., S. Murosaki, Y. Yamamoto, K. Muroyama, Y. Miwa, A. Fujishima, and B. Lynch. 2009. Safety studies of LP20 powder produced from heat-killed Lactobacillus plantarum L-137. Toxicol. Pharmacol. 54: 214-20.
32 Lash, B.W, T. H. Mysliwiec, and H. Gourama. 2005. Detection and partial characterization of a broad-range bacteriocin produced by Lactobacillus plantarum (ATCC 8014). Food Microbiol. 22: 199-204.   DOI   ScienceOn
33 Iijima, O. T., H. Takeda, Y. Komatsu, T. Matsumiya, and H. Takahashi. 2000. Atopic dermatitis in NC/Jic mice associated with Myobia musculi infestation. Comp. Med. 50: 225-228.
34 Kim, H. J., N. K. Lee, S. M. Cho, K. T. Kim, and H. D. Paik. 1999. Inhibition of spoilage and pathogenic bacteria by Lacticin NK24, a bacteriocin produced by Lactococcus lactis NK24 from fermented fish food. Korean J. Food Sci. Technol. 31: 1035-1043.