• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.230-238
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• 2014

The purpose of this study was to develop the cost-effective and efficiency seed longevity prediction method of rice (Oryza sativa L.) germplasm for viability monitoring. To find an optimum predicting method for rice seed longevity at genebank, an accelerated ageing (AA) test, a controlled deterioration (CD) test and a dry-heat treatment (DHT) were conducted to the four groups of rice germplasm based on ecotype, such as Indica, Japonica, Javanica and Tongil type. Among the three artificial aging treatments, the dry-heat treatment of 36 hours at $90^{\circ}C$ is suggested as a routine predictive test method of rice germplasm longevity at a genebank. The distribution of germination rate on 3,066 accessions which conserved 26.5 years at $4^{\circ}C$ showed similar trend with the result of distribution by dry-heat treatment at $90^{\circ}C$ on 36 hours using 106 accessions of rice selected samples which composed four ecotype groups. The results show that the dry-heat treatment affect not only predicting the rice seed longevity but also determining effective interval for monitoring germination of rice germplasm in genebanks.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.216-222
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• 2014

The purpose of this study was to know the seed longevity of rice (Oryza sativa L.) germplasm for effective viability monitoring. The longevity was determined via germination tests of 3,066 accessions of rice germplasm from the National Agrobiodiversity Center, Rural Development Administration, Korea. The rice germplasm accessions have been conserved at a mid-term storage ($4^{\circ}C$, 30% RH) in plastic bottle containing dehydrated (blue) silica-gel and long-term storage ($-18^{\circ}C$, 35% RH) in hermetically sealed metal can on either sides for 25~26.5 years. The final germination percentages of 3,066 rice germplasm accessions of $6.5{\pm}1.0%$ seed moisture content with 94% initial germination stored at $4^{\circ}C$ for 26.5 years declined to 47% while at $-18^{\circ}C$ for 25 years maintained high germinability as 93%. Germination time courses, which represent the average performance of rice ecotypes stored at $4^{\circ}C$ and 30% RH, were fitted regression equation, to calculate the time at which germination characteristically declined to 50% ($P_{50}$). These $P_{50}$ values of Indica, Japonica, Javanica and Tongil type in rice were 39.9, 22.9, 25.4 and 31.8 years, respectively. The rice germplasm stored at $4^{\circ}C$ could be clustered in 4 groups using quartile of final germination after 26.5 years storage. The seed longevity ($P_{50}$) of each group was estimated by regression equation of changed germination percentages according to storage periods. The $P_{50}$ values of group I, group II, group III and group IV were 21.1, 23.6, 30.0 and 75.7 years.

• Journal of Applied Biological Chemistry
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• v.59 no.4
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• pp.281-284
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• 2016

To handle the development of antifungal drug resistance, the development of new structural modules and new modes of action for antifungals have been highlighted recently. Here, the antifungal activity of quinonemethidal triterpenoids such as celastrol, dihydrocelastrol, iguestein, pristimerin, and tingenone isolated from Tripterygium regelii were identified (MIC $0.269-19.0{\mu}M$). C. glabrata was the most susceptible to quinonemethide among the tested fungi. Furthermore, quinonemethide suppressed cyctochrome c peroxidase expression dramatically, decreasing fungal viability caused by the accumulation of hydrogen peroxide. Thus, cyctochrome c peroxidase downregulation of quinonemethide may be a key mode of action for antifungals.

• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.256-262
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• 2017

BACKGROUND: Seed of perilla (Perilla frutescens var. japonica Hara) is short-lived in conventional storage conditions. For long-term conservation of plant species, cryopreservation is the method currently available. This study was performed to find out reliable methods for a long-term storage of seeds of perilla as a genetic resource. METHODS AND RESULTS: Using seeds of 9 perilla cultivars, the effects of desiccation, aging, and cryopreservation on seed germinability and ascorbate peroxidase activity in the seeds were investigated. Initial germinability of the seeds was various, and dry seeds of all cultivars survived cryopreservation without loss of viability. The highest germination was achieved at 4-5% moisture content, and stimulatory effect of cryogenic temperature on the seed germination was observed in some cultivars. Accelerated aging of perilla seeds led to reduction in germination and ascorbate peroxidase activity, and the susceptibility of seeds to aging was different among the tested cultivars. No significant difference in germination was observed for the aged seeds of control and liquid nitrogen exposed. CONCLUSION: The results of this study suggest that cryopreservation at 4-5% moisture content would be a suitable method for long-term conservation of perilla seeds without detrimental effects on germination.

• Journal of Veterinary Clinics
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• v.35 no.2
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• pp.39-45
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• 2018

We evaluated the effects of green tea extract (GTE) supplementation at different dilution steps on boar sperm freezing and in vitro fertilization. Sperm intracellular hydrogen peroxide ($H_2O_2$), motility, viability, acrosome integrity and morphology were determined. In addition, sperm IVF parameters (penetration and monospermy) and glutathione (GSH) levels of presumptive zygotes (PZs) were evaluated. Semen was diluted in lactose egg yolk (LEY) and cooled at $5^{\circ}C$ for 3 h (first dilution step) and then diluted in LEY with 9% glycerol and maintained at $5^{\circ}C$ for 30 min (second dilution step). Four experimental groups were compared: first and second dilution steps without GTE (control), first dilution step with GTE (Step 1), second dilution step with GTE (Step 2) and first and second dilution step with GTE (Step 1+2). The spermatozoa were frozen in nitrogen vapor. Higher sperm motility, viability and acrosome integrity after thawing were observed in Step 1, Step 2 and Step 1+2 groups compared with the control (P < 0.05). Lower $H_2O_2$ level was observed in Step 1+2 compared with control and Step 1 (P < 0.05). For IVF, matured oocytes were co-cultured with spermatozoa frozen according to the experimental groups. GSH levels of PZs were significantly higher in Step 2 and Step 1+2 than in control and Step 1 (P < 0.05) without a significant difference in IVF parameters. In conclusion, supplementation with GTE in both first and second dilution steps during the freezing process resulted in better boar sperm cryopreservation and might be beneficial for further embryo development.

• Journal of Biosystems Engineering
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• v.37 no.6
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• pp.411-419
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• 2012

Purpose: Nondestructive evaluation of seed viability is a highly demanded technique in the seed industry. In this study, hyperspectral imaging system was used for discrimination of viable and non-viable radish seeds. Method: The spectral data with the range from 400 to 1000 nm measured by hyperspectral reflectance imaging system were used. A calibration and a test models were developed by partial least square discrimination analysis (PLS-DA) for classification of viable and non-viable radish seeds. Either each data set of visible (400~750 nm) and NIR (750~1000 nm) spectra and the spectra of the combined spectral ranges were used for developing models. Results: The discrimination accuracy of calibration was 84% for visible range and 76.3% for NIR range. The discrimination accuracy of test was 84.2% for visible range and 75.8% for NIR range. The discrimination accuracies of calibration and test with full range were 92.2% and 92.5%, respectively. The resultant images based on the optimal PLS-DA model showed high performance for the discrimination of the nonviable seeds from the viable seeds with the accuracy of 95%. Conclusions: The results showed that hyperspectral reflectance imaging has good potential for discriminating nonviable radish seeds from massive amounts of viable seeds.

• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.602-608
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• 1999

Two-day old house fly adults were exposed to six insect growth regulators, flufenoxuron, teflubenzuron, triflumuron, diflubenzuron, methoxyfenozide, tebufenozide, as a feed additive (milk+5% sugar+chemical) in the laboratory for 6 days. The number of eggs deposited by the exposed-adults, viability of the eggs, and $F_1$ larval development were checked. All the IGRs tested were found to have no adverse effect on the reproduction of house fly, except methoxyfenozide (210ppm). The most effective inhibitor to egg hatch was flufenoxuron, followed by teflubenzuron, triflumuron, and diflubenzuron. Exposure to flufenoxuron (over 5ppm), teflubenzuron (over 25ppm), triflumuron (over 125ppm), and diflubenzuron (over 125ppm) reduced egg hatchability to 0 to 1.3%, but lower concentrations of these IGRs were less effective (6.3 to 46.3% egg hatchability). Almost all the larvae emerged from eggs deposited by the adults exposed to diflubenzuron (62.5ppm) and teflubenzuron (12.5ppm) failed to develop into pupae, causing total mortalities of 98% and 100%, respectively. However, two IGRs, methoxyfenozide and tebufenozide, did not inhibit egg hatch and $F_1$ larval development, except methoxyfenozide (210ppm) treatment These results suggest that these 4 IGRs may be used in the development of autosterilization system for house fly control. However, further work is required to develop delivery systems capable of transferring an effective dose to the fly under field conditions.

• Korean Journal of Plant Resources
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• v.28 no.6
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• pp.718-726
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• 2015

In this study, the inhibitory activities of fifty plant extracts on IgE-mediated degranulation in the rat basophilic leukemia cell line (RBL-2H3 cells) were measured; the release of interleukin (IL)-4 and β-hexosaminidase from IgE-sensitized cells treated with the plant extracts was measured; and the effects of the plant extracts on cell viability were tested. The results of the analysis of plant extracts at 20 μg/ml, including the aerial part of Magnolia sieboldii K. Koch, exhibited suppressive activities upon the release of IL-4. Furthermore, several plant extracts including methanol extracted from Lindera erythrocarpa Makino (aerial part) at the same concentration significantly inhibited the release of β-hexosaminidase. Twenty-six of the plant extracts, including methanol extract of Weigela subsessilis (Nakai) L. H. Bailey (branch), showed a cell proliferation effect of over 80% at 100 μg/ml. In conclusion, the results suggest that the leaf/stem of Geum japonicum Thunb. and the stamen/ovary of Nelumbo nucifera Gaertn., which exhibited effective inhibition on β-hexosaminidase release and IL-4 release from mast cells and showed high cell viability, could be useful candidates as anti-allergy materials.

• Journal of Veterinary Clinics
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• v.36 no.5
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• pp.259-265
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• 2019

This study was designed to investigate the effects of alpha-glucosyl rutin (G-rutin) and its comparative effects with other antioxidants (glutathione: GSH, catalase: CATA and beta-mercaptoethanol : ${\beta}ME$) on dog sperm freezing. In the first experiment (E1), the spermatozoa were diluted in freezing extender supplemented with 0 (control), 0.001, 0.01, or 0.1% G-rutin and frozen using liquid nitrogen ($LN_2$). The progressive motility, reactive oxygen species (ROS) level and apoptosis of spermatozoa were assessed after sperm thawing at $37^{\circ}C$ for 25 sec. In the second experiment (E2), 0.1% G-rutin group was compared with 10 mM ${\beta}-ME$, $5{\mu}M$ GSH and $50{\mu}M$ CATA groups by assaying progressive motility, viability and gene expression of Bcl-2 and SMCP after sperm freezing and thawing. In E1, 0.1% G-rutin group showed higher (P < 0.05) post-thaw progressive motility and lower (P < 0.05) ROS levels. In E2, the expressions of SMCP in G-rutin group were higher (P < 0.05) than in CATA group while Bcl-2 expression of G-rutin group was higher (P < 0.05) than ${\beta}-ME$ and CATA groups. However, there were no significant differences in progressive motility and viability. Therefore, we suggest that G-rutin can be used as a potentially antioxidative supplement in dog sperm freezing extender on the basis of gene expression related to motility and apoptosis as well as ROS level.

• Nutrition Research and Practice
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• v.12 no.2
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• pp.93-100
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• 2018

BACKGROUND/OBJECTIVE: Oxidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. The aim of present study was to investigate the neuroprotective effects of perilla oil (PO) and its active component, alpha-linolenic acid (ALA), against hydrogen peroxide $(H_2O_2)$-induced oxidative stress in SH-SY5Y neuronal cells. MATERIALS/METHODS: The SH-SY5Y human neuroblastoma cells exposed to $250{\mu}M$ $H_2O_2$ for 24 h were treated with different concentrations of PO (25, 125, 250 and $500{\mu}g/mL$) and its major fatty acid, ALA (1, 2.5, 5 and $25{\mu}g/mL$). We examined the effects of PO and ALA on $H_2O_2$-induced cell viability, lactate dehydrogenase (LDH) release, and nuclear condensation. Moreover, we determined whether PO and ALA regulated the apoptosis-related protein expressions, such as cleaved-poly ADP ribose polymerase (PARP), cleaved caspase-9 and -3, BCL-2 and BAX. RESULTS: Treatment of $H_2O_2$ resulted in decreased cell viability, increased LDH release, and increase in the nuclei condensation as indicated by Hoechst 33342 staining. However, PO and ALA treatment significantly attenuated the neuronal cell death, indicating that PO and ALA potently blocked the $H_2O_2$-induced neuronal apoptosis. Furthermore, cleaved-PARP, cleaved caspase-9 and -3 activations were significantly decreased in the presence of PO and ALA, and the $H_2O_2$-induced up-regulated BAX/BCL-2 ratio was blocked after treatment with PO and ALA. CONCLUSIONS: PO and its main fatty acid, ALA, exerted the protective activity from neuronal oxidative stress induced by $H_2O_2$. They regulated apoptotic pathway in neuronal cell death by alleviation of BAX/BCL-2 ratio, and down-regulation of cleaved-PARP and cleaved caspase-9 and -3. Although further studies are required to verify the protective mechanisms of PO and ALA from neuronal damage, PO and ALA are the promising agent against oxidative stress-induced apoptotic neuronal cell death.