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http://dx.doi.org/10.7732/kjpr.2015.28.6.718

Inhibition Activity of Plants on IgE-mediated Degranulation of RBL-2H3 Cells  

Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Jeong, Hye-Gwang (College of Pharmacy, Chungnam National University)
Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Lee, Jeong-Hoon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Choi, Jehun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Noh, Hyung-Jun (Technology Cooperation Bureau, Rural Development Administration)
Lee, Jae-Won (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Kim, Seung-Yu (Department of Horticultural Crop Research, National Institute of Horticultural & Herbal Science)
Ahn, Young-Sup (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Publication Information
Korean Journal of Plant Resources / v.28, no.6, 2015 , pp. 718-726 More about this Journal
Abstract
In this study, the inhibitory activities of fifty plant extracts on IgE-mediated degranulation in the rat basophilic leukemia cell line (RBL-2H3 cells) were measured; the release of interleukin (IL)-4 and β-hexosaminidase from IgE-sensitized cells treated with the plant extracts was measured; and the effects of the plant extracts on cell viability were tested. The results of the analysis of plant extracts at 20 μg/ml, including the aerial part of Magnolia sieboldii K. Koch, exhibited suppressive activities upon the release of IL-4. Furthermore, several plant extracts including methanol extracted from Lindera erythrocarpa Makino (aerial part) at the same concentration significantly inhibited the release of β-hexosaminidase. Twenty-six of the plant extracts, including methanol extract of Weigela subsessilis (Nakai) L. H. Bailey (branch), showed a cell proliferation effect of over 80% at 100 μg/ml. In conclusion, the results suggest that the leaf/stem of Geum japonicum Thunb. and the stamen/ovary of Nelumbo nucifera Gaertn., which exhibited effective inhibition on β-hexosaminidase release and IL-4 release from mast cells and showed high cell viability, could be useful candidates as anti-allergy materials.
Keywords
Allergy; Degranulation; β -hexosaminidase; Mast cell; Interleukin-4;
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Times Cited By KSCI : 4  (Citation Analysis)
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