Browse > Article
http://dx.doi.org/10.17555/jvc.2018.04.35.2.39

Effects of Green Tea (Camellia sinensis) Extract Supplementation at Different Dilution Steps on Boar Sperm Cryopreservation and in vitro Fertilization  

Park, Sang-Hyoun (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
Jeon, Yubyeol (Department of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Chonbuk National University)
Yu, Il-Jeoung (Department of Theriogenology and Reproductive Biotechnology, College of Veterinary Medicine and Bio-safety Research Institute, Chonbuk National University)
Publication Information
Journal of Veterinary Clinics / v.35, no.2, 2018 , pp. 39-45 More about this Journal
Abstract
We evaluated the effects of green tea extract (GTE) supplementation at different dilution steps on boar sperm freezing and in vitro fertilization. Sperm intracellular hydrogen peroxide ($H_2O_2$), motility, viability, acrosome integrity and morphology were determined. In addition, sperm IVF parameters (penetration and monospermy) and glutathione (GSH) levels of presumptive zygotes (PZs) were evaluated. Semen was diluted in lactose egg yolk (LEY) and cooled at $5^{\circ}C$ for 3 h (first dilution step) and then diluted in LEY with 9% glycerol and maintained at $5^{\circ}C$ for 30 min (second dilution step). Four experimental groups were compared: first and second dilution steps without GTE (control), first dilution step with GTE (Step 1), second dilution step with GTE (Step 2) and first and second dilution step with GTE (Step 1+2). The spermatozoa were frozen in nitrogen vapor. Higher sperm motility, viability and acrosome integrity after thawing were observed in Step 1, Step 2 and Step 1+2 groups compared with the control (P < 0.05). Lower $H_2O_2$ level was observed in Step 1+2 compared with control and Step 1 (P < 0.05). For IVF, matured oocytes were co-cultured with spermatozoa frozen according to the experimental groups. GSH levels of PZs were significantly higher in Step 2 and Step 1+2 than in control and Step 1 (P < 0.05) without a significant difference in IVF parameters. In conclusion, supplementation with GTE in both first and second dilution steps during the freezing process resulted in better boar sperm cryopreservation and might be beneficial for further embryo development.
Keywords
Dilution step; Freezing; Green tea extract; Glutathione; Reactive oxygen species;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Park SH, Yu IJ. Evaluation of Toxicity of Green Tea Extract in Chilled Boar Spermatozoa. J Emb Trans 2015; 30: 1-6.   DOI
2 Park SH, Yu IJ. Effect of dibutyryl cyclic adenosine monophosphate on reactive oxygen species and glutathione of porcine oocytes, apoptosis of cumulus cells, and embryonic development. Zygote 2013; 21: 305-313.   DOI
3 Corcuera BD, Marigorta P, Sagues A, Saiz-Cidoncha F, Perez-Gutierrez JF. Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa. Theriogenology 2007; 67: 1150-1157.   DOI
4 de Matos DG, Furnus CC. The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development: Effect of beta-mercaptoethanol, cysteine and cysteine. Theriogenology 2000; 53: 761-771.   DOI
5 Drobnis EZ, Crowe LM, Berger T, Anchordoguy TJ, Overstreet JW, Crowe JH. Cold shock damage is due to lipid phase transitions in cell membranes: a demonstration using sperm as a model. J Exp Zool 1993; 265: 432-437.   DOI
6 Edwards JL, King WA, Kawarsky SJ, Ealy AD. Responsiveness of early embryos to environmental insults: potential protective roles of HSP70 and glutathione. Theriogenology 2001; 55: 209-223.   DOI
7 Gil MA, Alminana C, Cuello C, Parrilla I, Roca J, Vazquez JM, Martinez EA. Brief coincubation of gametes in porcine in vitro fertilization: role of sperm:oocyte ratio and postcoincubation medium. Theriogenology 2007; 67: 620-626.   DOI
8 Fraser L, Strzezek J. Effects of freezing-thawing on DNA integrity of boar spermatozoa assessed by the neutral comet assay. Reprod Domest Anim 2005; 40: 530-536.   DOI
9 Gadea J, Selles E, Marco MA. The predictive value of porcine seminal parameters on fertility outcome under commercial conditions. Reprod Domest Anim 2004; 39: 303-308.   DOI
10 Gadea J, Selles E, Marco MA, Coy P, Matas C, Romar R, Ruiz S. Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 2004; 62: 690-701.   DOI
11 Bailey JL, Bilodeau JF, Cormier N. Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. J Androl 2000; 21: 1-7.
12 Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev 2000; 55: 282-288.   DOI
13 Brezezi ska-Slebodzi ska E, Slebodzi ski AB, Pietras B, Wieczorek G. Antioxidant effect of vitamin E and glutathione on lipid peroxidation in boar semen plasma. Biol Trace Elem Res 1995; 47: 69-74.   DOI
14 Burruel V, Klooster K, Barker CM, Pera RR, Meyers S. Abnormal early cleavage events predict early embryo demise: sperm oxidative stress and early abnormal cleavage. Sci Rep 2014; 13: 6598.
15 Circu ML, Aw TY. Glutathione and apoptosis. Free Radic Res 2008; 42: 689-706.   DOI
16 Cerolini S, Maldjian A, Surai P, Noble R. Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim Reprod Sci 2000; 58: 99-111.   DOI
17 Chatterjee S, Gagnon C. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing and thawing. Mol Reprod Dev 2001; 59: 451-458.   DOI
18 Chen L, Yang X, Jiao H, Zhao B. Tea catechins protect against lead-induced ROS formation, mitochondrial dysfunction and calcium dysregulation in PC12 cells. Chem Res Toxicol 2003; 16: 1155-1161.   DOI
19 Armstrong JS, Rajasekaran M, Chamulitrat W, Gatti P, Hellstrom WJ, Sikka SC. Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Radic Biol Med 1999; 26: 869-880.   DOI
20 Aziz N, Agarwal A, Lewis-Jones I, Sharma RK, Thomas AJ Jr. Novel associations between specific sperm morphological defects and leukocytospermia. Fertil Steril 2004; 82: 621-627.   DOI
21 Abeydeera LR, Wang WH, Cantley TC, Prather RS, Day BN. Presence of beta-mercaptoethanol can increase the glutathione content of pig oocytes matured in vitro and the rate of blastocyst development after in vitro fertilization. Theriogenology 1998; 50: 747-756.   DOI
22 Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003; 79: 829-843.   DOI
23 Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennings Z, Irvine DS. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod 1998; 59: 1037-1046.   DOI
24 Yamaguchi S, Funahashi H, Murakami T. Improved fertility in gilts and sows after artificial insemination of frozenthawed boar semen by supplementation of semen extender with caffeine and $CaCl_2$. J Reprod Dev 2009; 55: 645-649.   DOI
25 Almlid T, Johnson LA. Effects of glycerol concentration, equilibration time and temperature of glycerol addition on post-thaw viability of boar spermatozoa frozen in straws. J Anim Sci 1988; 66: 2899-2905.   DOI
26 Amarowicz R, Naczk M, Shahidi F. Antioxidant activity of various fractions of non-tannin phenolics of canola hulls. J Agric Food Chem 2000; 48: 2755-2759.   DOI
27 Yamaguchi S, Funahashi H. Effect of the addition of betamercaptoethanol to a thawing solution supplemented with caffeine on the function of frozen-thawed boar sperm and on the fertility of sows after artificial insemination. Theriogenology 2012; 77: 926-932.   DOI
28 Yang CS. Tea and health, Nutrition 1999; 15: 946-949.   DOI
29 Yu IJ, Leibo SP. Recovery of motile, membrane-intact spermatozoa from canine epididymides stored for 8 days at $4^{\circ}C$. Therigenology 2002; 57: 1179-1190.   DOI
30 Yu I, Songsasen N, Godke RA, Leibo SP. Differences among dogs in response of their spermatozoa to cryopreservation using various cooling and warming rates. Cryobiology 2002; 44: 62-78.   DOI
31 Yu YG, Shim MH, Chae JW. The anti-oxidative stress effect of antioxidants in the cell using DCFH-DA. J Soc Cos Sci Ko 2002; 28: 12-57.
32 Purdy PH. The post-thaw quality of ram sperm held for 0 to 48 h at 5 degrees C prior to cryopreservation. Anim Reprod Sci 2006; 93: 114-123.   DOI
33 Zaveri NT. Green tea and its polyphenolic catechins: medicinal uses in cancer and noncancer applications. Life Sci 2006; 78: 2073-2080.   DOI
34 Park SH, Yu IJ, Effects of Green Tea Extract on Sperm Quality, Reactive Oxygen Species and Lipid Peroxidation in Long-term Liquid Preservation of Boar Spermatozoa. J Vet Clin 2016; 33: 356-361.   DOI
35 Pena FJ, Johannisson A, Wallgren M, Rodriguez Martinez H. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Anim Reprod Sci 2003; 78: 85-98.   DOI
36 Pursel VG, Johnson LA. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure. J Anim Sci 1975; 40: 99-102.   DOI
37 Rahman Md A, Park SH, Yu IJ. Effect of monosaccharides in glycerol-free tris extender on reactive oxygen species and apoptosis in dog sperm cryopreservation. Cryo Letters 2016; 38: 51-57.
38 Roca J, Rodriguez MJ, Gil MA, Carvajal G, Garcia EM, Cuello C, Vazquez JM, Martinez EA. Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. J Androl 2005; 26: 15-24.
39 Silva PF, Gadella BM. Detection of damage in mammalian sperm cells. Theriogenology 2006; 65: 958-978.   DOI
40 Wang A, Fanning L, Anderson DJ, Loughlin KR. Generation of reactive oxygen species by leukocytes and sperm following exposure to urogenital tract infection. Arch Androl 1997; 39: 11-17.   DOI
41 Hammerstedt RH, Graham JK, Nolan JP. Cryopreservation of mammalian sperm: what we ask them to survive. J Androl 1990; 11: 73-88.
42 Watson PF. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci 2000; 60-61: 481-492.   DOI
43 Whitaker BD, Casey SJ, Taupier R. N-acetyl-l-cysteine supplementation improves boar spermatozoa characteristics and subsequent fertilization and embryonic development. Reprod Domest Anim 2012; 47: 263-268.   DOI
44 Guthrie HD, Welch GR. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescenceactivated flow cytometry. J Anim Sci 2006; 84: 2089-2100.   DOI
45 Jeong YJ, Kim MK, Song HJ, Kang EJ, Ock SA, Kumar BM, Balasubramanian S, Rho GJ. Effect of alpha-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology 2009; 58: 181-189.   DOI
46 Malo C, Gil L, Cano R, Martinez F, Gale I. Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology 2011; 75: 1735-1741.   DOI
47 Malo C, Gil L, Gonzalez N, Martinez F, Cano R, de Blas I, Espinosa E. Anti-oxidant supplementation improves boar sperm characteristics and fertility after cryopreservation: comparison between cysteine and rosemary (Rosmarinus officinalis). Cryobiology 2010; 61: 142-147.   DOI
48 McCarthy MJ, Baumber J, Kass PH, Meyers SA. Osmotic stress induces oxidative cell damage to rhesus macaque spermatozoa. Biol Reprod 2010; 82: 644-651.   DOI
49 Mammoto A, Masumoto N, Tahara M, Ikebuchi Y, Ohmichi M, Tasaka K, Miyake A. Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice. Biol Reprod 1996; 55: 1063-1068.   DOI