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http://dx.doi.org/10.5338/KJEA.2017.36.4.34

Response of Germination Rate and Ascorbate Peroxidase Activity to Cryopreservation of Perilla (Perilla frutescens) Seeds with Variable Initial Viabilities  

Lee, Young-yi (National Agrobiodiveristy Center, National Institute of Agricultural Science, Rural Development Administration)
Lee, Myeong-hee (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration)
Yi, Jung-yoon (National Agrobiodiveristy Center, National Institute of Agricultural Science, Rural Development Administration)
Lee, Tae-yoon (Department of Environmental Horticulture, College of Natural Science, University of Seoul)
Son, Eun-ho (National Agrobiodiveristy Center, National Institute of Agricultural Science, Rural Development Administration)
Park, Hong-jae (National Agrobiodiveristy Center, National Institute of Agricultural Science, Rural Development Administration)
Publication Information
Korean Journal of Environmental Agriculture / v.36, no.4, 2017 , pp. 256-262 More about this Journal
Abstract
BACKGROUND: Seed of perilla (Perilla frutescens var. japonica Hara) is short-lived in conventional storage conditions. For long-term conservation of plant species, cryopreservation is the method currently available. This study was performed to find out reliable methods for a long-term storage of seeds of perilla as a genetic resource. METHODS AND RESULTS: Using seeds of 9 perilla cultivars, the effects of desiccation, aging, and cryopreservation on seed germinability and ascorbate peroxidase activity in the seeds were investigated. Initial germinability of the seeds was various, and dry seeds of all cultivars survived cryopreservation without loss of viability. The highest germination was achieved at 4-5% moisture content, and stimulatory effect of cryogenic temperature on the seed germination was observed in some cultivars. Accelerated aging of perilla seeds led to reduction in germination and ascorbate peroxidase activity, and the susceptibility of seeds to aging was different among the tested cultivars. No significant difference in germination was observed for the aged seeds of control and liquid nitrogen exposed. CONCLUSION: The results of this study suggest that cryopreservation at 4-5% moisture content would be a suitable method for long-term conservation of perilla seeds without detrimental effects on germination.
Keywords
Ascorbate peroxidase; Cryopreservation; Germination; Perilla; Seed;
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