• 제목/요약/키워드: Research and Development Activities

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Antioxidant and Cholinesterase Inhibitory Activities of Antarctic Krill Eupausia superba

  • Yoon, Na-Young;Xie, Chengliang;Shim, Kil-Bo;Kim, Yeon-Kye;Lee, Doo-Seog;Yoon, Ho-Dong
    • Fisheries and Aquatic Sciences
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    • 제14권4호
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    • pp.289-293
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    • 2011
  • The antioxidant and cholinesterase inhibitory activities of methanol, pretanol, and acetone extracts of Eupausia superba were investigated and their bioactivities compared. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic acid] ($ABTS^+$) radical-scavenging activities and reducing power assays were used to determine antioxidant activities, and Ellman's colorimetric methods were applied to evaluate cholinesterase inhibitory activity. Although all extracts were positive, Acetone extract of E. superba showed the highest activities. However, these showed moderate or no inhibitory activity against butyrylcholinesterase. Moreover, the total carotenoid contents of the organic solvent extracts followed the same order as their antioxidant and acetylcholinesterase inhibitory activities. These results suggest that E. superba is a potential source of natural antioxidants and cholinesterase inhibitors.

Antioxidant and Cholinesterase Inhibitory Activities of the By-products of Three Pandalid Shrimps

  • Kim, Sang-Bo;Yoon, Na Young;Shi, Kil Bo;Lim, Chi-Won
    • Fisheries and Aquatic Sciences
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    • 제17권4호
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    • pp.421-425
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    • 2014
  • The antioxidant and cholinesterase inhibitory activities of the acetone and dichloromethane ($CH_2Cl_2$) extracts of the by-products (heads, shells, and tails) of Pandalus borealis, Pandalus hypsinotus, and Pandalopsis japonica belonging to the family Pandalidae were investigated and their bioactivities were compared. The antioxidant and cholinesterase inhibitory activities of the organic solvent extracts of three shrimp by-products were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-Azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] ($ABTS^+$) radical scavenging activities, reducing power and xanthine oxidase (XO) inhibitory activity assays and Ellman's colorimetric method. The extracts of P. hypsinotus exhibited the highest antioxidant and cholinesterase inhibitory activities. The acetone extracts showed more potent activities toward antioxidant and cholinesterase inhibition compared with the $CH_2Cl_2$ extracts. Furthermore, the total carotenoid contents of the acetone extracts were higher than those of the $CH_2Cl_2$ extracts. Thus, the carotenoid contents may affect antioxidant and cholinesterase inhibition. Our results suggest that the shrimp by-products could act as a nutraceutical agent to prevent oxidative stress and Alzheimer's disease.

Antioxidant and Angiotensin I Converting Enzyme Inhibitory Activities of Red Snow Crab Chionoecetes japonicas Shell Hydrolysate by Enzymatic Hydrolysis

  • Yoon, Na Young;Shim, Kil-Bo;Lim, Chi-Won;Kim, Sang-Bo
    • Fisheries and Aquatic Sciences
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    • 제16권4호
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    • pp.237-242
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    • 2013
  • We investigated the antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities of red snow crab Chionoecetes japonicas shell (RSCS) hydrolysate by enzymatic hydrolysis and its molecular weight cut-off fractions. The RSCS hydrolysate was fractionated through two ultrafiltration membranes of 3 and 10 kDa cut-offs. Three fractions (<3 kDa, 3-10 kDa, and >10 kDa) were evaluated for total amino acid composition, antioxidant activities using 2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic acid] ($ABTS^+$) radical scavenging and superoxide dismutase (SOD)-like activities and reducing power assays, and ACE inhibitory activity using Hou's method. Although all fractions showed activity, the <3 kDa fraction of RSCS hydrolysate exhibited the greatest $ABTS^+$ radical scavenging, SOD-like and ACE inhibitory activities. However, these fractions exhibited low reducing power. These results suggest that the low-molecular-weight enzymatic hydrolysate of RSCS could be used as a functional ingredient to control oxidative stress and ACE activity.

Evaluation of the Activities of Antioxidant Enzyme and Lysosomal Enzymes of the Longissimus dorsi Muscle from Hanwoo (Korean Cattle) in Various Freezing Conditions

  • Kang, Sun Moon;Kang, Geunho;Seong, Pil-Nam;Park, Beomyoung;Kim, Donghun;Cho, Soohyun
    • 한국축산식품학회지
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    • 제34권6호
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    • pp.742-748
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    • 2014
  • This study was conducted to evaluate the activities of antioxidant enzyme (glutathione peroxidase (GSH-Px)) and lysosomal enzymes (alpha-glucopyranosidase (AGP) and beta-N-acetyl-glucosaminidase (BNAG)) of the longissimus dorsi (LD) muscle from Hanwoo (Korean cattle) in three freezing conditions. Following freezing at -20, -60, and $-196^{\circ}C$ (liquid nitrogen), LD samples (48 h post-slaughter) were treated as follows: 1) freezing for 14 d, 2) 1 to 4 freeze-thaw cycles (2 d of freezing in each cycle), and 3) refrigeration ($4^{\circ}C$) for 7 d after 7 d of freezing. The control was the fresh (non-frozen) LD. Freezing treatment at all temperatures significantly (p<0.05) increased the activities of GSH-Px, AGP, and BNAG. The $-196^{\circ}C$ freezing had similar effects to the $-20^{\circ}C$ and $-60^{\circ}C$ freezing. Higher (p<0.05) enzymes activities were sustained in frozen LD even after 4 freeze-thaw cycles and even for 7 d of refrigeration after freezing. These findings suggest that freezing has remarkable effects on the activities of antioxidant enzyme and lysosomal enzymes of Hanwoo beef in any condition.

국내산 7종 다슬기 추출물의 생리활성 특성 비교 (Biological Activities of Seven Melania Snails in Korea)

  • 김연계;문호성;이문희;박미주;임치원;박희연;박진일;윤호동;김대희
    • 한국수산과학회지
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    • 제42권5호
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    • pp.434-441
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    • 2009
  • This study was conducted to compare the biological activities of 7 melania snails from the family Pleuroceridae (Semisulcospira coreana, Koreanomelania nodifila, Semisulcospira forticosta, Koreoleptoxis globus ovalis, Semisulcospira libertina, Semisulcospira tegulata and Semisulcospira gottschei) in Korea. Among the 7 species, S. coreana, Korean. nodifila, S. forticosta and S. gottschei showed over 80% cytotoxicities on three cancer cell lines (SNU-1, A549 and Hep 3B) compared to the non-treatment, whereas S. libertina and S. tegulata showed almost no growth inhibition activities on the same cancer cell lines. In relation to ACE inhibition activity, only S. coreana, Korean. nodifila, and S. forticosta showed over 60% ACE inhibition activities, whereas other melania snails exhibited inhibition activities of lower than 25%. DPPH radical scavenging activities were also determined, and used to categories melania snails into three groups based on Duncan's multiple range test at P<0.05. The amount of TNF-${\alpha}$ produced by in vitro mouse peritoneal macrophage was determined according to bioactivity on L-929 cells. Three melania snails, S. coreana, Korean. nodifila and S. gottschei, exhibited 95.2%, 89.7% and 93.7% cell death(%) on L-929 cells, respectively. Glucose-6-phosphate dehydrogenase inhibitory activity was also obtained in the extract of S. coreana (31.9%) and Korean. nodifila (28.1%), showing that these extracts can be used as supplemental dietary health foods. In conclusion, we believe that the extracts of melania snails should be given due consideration in functional health food development.

PRA RESEARCH AND THE DEVELOPMENT OF RISK-INFORMED REGULATION AT THE U.S. NUCLEAR REGULATORY COMMISSION

  • Siu, Nathan;Collins, Dorothy
    • Nuclear Engineering and Technology
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    • 제40권5호
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    • pp.349-364
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    • 2008
  • Over the years, probabilistic risk assessment (PRA) research activities conducted at the U.S. Nuclear Regulatory Commission (NRC) have played an essential role in support of the agency's move towards risk-informed regulation. These research activities have provided the technical basis for NRC's regulatory activities in key areas; provided PRA methods, tools, and data enabling the agency to meet future challenges; supported the implementation of NRC's 1995 PRA Policy Statement by assessing key sources of risk; and supported the development of necessary technical and human resources supporting NRC's risk-informed activities. PRA research aimed at improving the NRC's understanding of risk can positively affect the agency's regulatory activities, as evidenced by three case studies involving research on fire PRA, human reliability analysis (HRA), and pressurized thermal shock (PTS) PRA. These case studies also show that such research can take a considerable amount of time, and that the incorporation of research results into regulatory practice can take even longer. The need for sustained effort and appropriate lead time is an important consideration in the development of a PRA research program aimed at helping the agency address key sources of risk for current and potential future facilities.

IEA SolarPACES 및 Task 활동 (Activities of IEA SolarPACES & Task Programs)

  • 강용혁;김종규;이현진
    • 한국태양에너지학회:학술대회논문집
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    • 한국태양에너지학회 2011년도 춘계학술발표대회 논문집
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    • pp.246-249
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    • 2011
  • SolarPACES is an international cooperative network bringing together teams of national exports from around the world to focus on the development and marketing of concentrating solar power systems (also known as solar thermal power systems). It is one of a number of collaborative programs, called Implementing Agreements, managed under the umbrella of the International Energy Agency to help find solutions to worldwide energy problems. Technology development is at the core of the work of SolarPACES. Member countries work together on activities aimed at solving the wide range of technical problems associated with commercialization of concentrating solar technology, including large-scale system tests and the development of advanced technologies, components, instrumentation, and systems analysis techniques. In addition to technology development, market development and building of awareness of the potential of concentrating solar technologies are key elements of the SolarPACES program The Implementing Agreement specifies broad "Tasks," or thematic areas of work SolarPACES currently has three ongoing tasks, focusing on concentrating solar electric power systems (Task I), solar chemistry research (Task II), and solar technology and applications (Task III). An Operating Agent, nominated by the ExCo, is responsible for overseeing the work of each task Each task maintains a detailed program of work that defines all task activities, including their objectives, participants, plans, and budgets. In addition to technical reports of the activities and their participants, accomplishments and progress are summarized in the SolarPACES annual report. Many SolarPACES activities involve close cooperation among member countries (either through sharing of task activities or, occasionally, cost-sharing), although some cooperation is limited to sharing of information and results with other participants. In this paper, structure, works, and members of SolarPACES and Korean activies in the SolarPACES are introduced.

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IEA SolarPACES 및 Task 활동 (Activities of IEA SolarPACES & Task Programs)

  • 강용혁;김종규;이현진
    • 한국태양에너지학회:학술대회논문집
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    • 한국태양에너지학회 2011년도 추계학술발표대회 논문집
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    • pp.320-323
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    • 2011
  • SolarPACES is an international cooperative network bringing together teams of national experts from around the world to focus on the development and marketing of concentrating solar power systems (also known as solar thermal power systems). It is one of a number of collaborative programs, called Implementing Agreements, managed under the umbrella of the International Energy Agency to help find solutions to worldwide energy problems. Technology development is at the core of the work of Solar PACES. Member countries work together on activities aimed at solving the wide range of technical problems associated with commercialization of concentrating solar technology, including large-scale system tests and the development of advanced technologies, components, instrumentation, and systems analysis techniques. In addition to technology development, market development and building of awareness of the potential of concentrating solar technologies are key elements of the Solar PACES program. The Implementing Agreement specifies broad "Tasks," or thematic areas of work. SolarPACES currently has three ongoing tasks, focusing on concentrating solar electric power systems (Task I), solar chemistry research (Task II), and solar technology and applications (Task III). An Operating Agent, nominated by the ExCo, is responsible for overseeing the work of each task. Each task maintains a detailed program of work that defines all task activities, including their objectives, participants, plans, and budgets. In addition to technical reports of the activities and their participants, accomplishments and progress are summarized in the SolarPACES annual report. Many SolarPACES activities involve close cooperation among member countries (either through sharing of task activities or, occasionally, cost-sharing), although some cooperation is limited to sharing of information and results with other participants. In this paper, structure, works, and members of SolarPACES and Korean activies in the SolarPACES are introduced.

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고등학생의 해양스포츠 활동과 사회성 발달에 관한 연구 (The study of high school student's activities of Marine Sports on the development of their sociality)

  • 지삼업
    • 수산해양교육연구
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    • 제18권3호
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    • pp.329-340
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    • 2006
  • The purpose of this research is to know the influence of high school students' activities of Marine Sports on the development of their sociality by comparing students in this activities with those who are not in this activities. The objects of this research are high school students who live in Busan at present. The total number of the object is 146, including 74 students who participated in this organization and 72 students who didn't participate in this organization. We have reached the following conclusion after analyzing the materials according to the methods and procedures of the research. In the domain of activity, the students who are in Marine sport activities are statistically different from those who are not. In the domain of stability, the students who are in Marine sport activities are meaningfully different from those who are not. In the domain of ascendancy, the students who are in Marine sport activities are meaningfully different from those who are not. In the domain of sociability, the students who are in Marine sport activities are meaningfully different from those who are not. In the domain of self-control, the students who are in Marine sport activities are meaningfully different from those who are not.

한국산 다슬기류(Semisulcospira spp.) 5종의 항산화 활성 (Antioxidant Activities of Five Melania Snails of the Genus Semisulcospira in Korea)

  • 이문희;김연계;문호성;김유아;윤나영;임치원;박희연;김대희
    • 한국수산과학회지
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    • 제43권3호
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    • pp.188-194
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    • 2010
  • The in vitro antioxidant activities of five melania snails in the genus Semisulcospira (S. coreana, S. forticosta, S. libertina, S. tegulata and S. gottschei) were tested in detail. The total phenolic contents of the snails ranged from $32.3{\pm}1.0$ to $87.9{\pm}6.9$ mg gallic acid equivalent (GAE)/g dry sample. The $EC_{50}$ values for the 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were $2.245{\pm}0.179$ mg/mL for S. coreana and $9.319{\pm}1.276$ mg/mL for S. gottschei and differed significantly (P<0.05) among the tested species. The superoxide dismutase (SOD)-like activity was highest for S. gottschei at 67.2% and lowest for S. forticosta at 4.7%. However, no significant differences among the species were recognized for the peroxynitrite anion scavenging activity. Comparing the correlation coefficients between the total phenolic contents and the DPPH radical and peroxynitrite anion scavenging activities, there was a low level relationship between each activity.