• Asian-Australasian Journal of Animal Sciences
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• 제19권10호
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• pp.1508-1513
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• 2006

Myostatin (MSTN) and insulin-like growth factors (IGFs) are a known inhibitor and stimulators of proliferation and differentiation of muscle cells, respectively. The present study was performed to investigate the relationship of MSTN-induced growth inhibition to expression of the IGF system components and myogenin, a muscle cell-specific transcription factor, in rat L6 myoblasts. The L6 cells transfected with a green fluorescent protein-MSTN plasmid expression construct had a 47% less cell number than mock-transfected cells after 3-d serum-free culture, accompanied by delayed differentiation which was suggested by inhibited aggregation of cells. Moreover, cells transfected with the expression construct had decreased expression of IGF-II and myogenin genes, but not IGF-I or its receptor genes, as examined by reverse transcription-polymerase chain reaction. The reduced mitosis of the L6 cells transfected with the MSTN-expression construct increased following an addition of either IGF-I or IGF-II to the culture medium, but not to the level of mock-transfected cells. By contrast, myogenin gene expression in these cells increased after the addition of either IGF to the level of mock-transfected cells. Collectively, these results suggest that the inhibitory effect of MSTN on L6 cell proliferation and differentiation is likely to be partly mediated by serially suppressed expression of IGF-II and myogenin genes, not IGF-I gene.

• 한국지역지리학회지
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• 제10권4호
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• pp.833-850
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• 2004

사회 각 부문의 이분법적인 분화가 근대성의 특징이었다면, 탈근대성 혹은 포스트모더니즘에서는 경계가 소멸되고 다양화되는 탈분화가 진행된다. 최근의 박물관들은 단순히 유물을 보관하는 근대적 공간에서 벗어나 여러 가지 사회문화적 요구를 수용하는 기능적 공간으로 탈근대화 되고 있다. 근대적 공간으로 탄생한 한국의 박물관이 문화 경관으로서 탈근대화 되는 양상을 알기 위해서는 역사적 형성 과정을 살펴보는 것이 필요하다. 이에 본 연구는 한국 박물관의 역사적 변천에서 나타나는 근대성과 탈근대성을 분화와 탈분화의 관점에서 살펴보았다. 이를 위해서 설립 주체의 유형별 변화를 토대로 한국 박물관의 역사적 변천과정을 근대적 박물관의 설립기(1945$\sim$1974), 탈근대적 박물관의 설립기(1975$\sim$1989), 탈근대적 박물관의 확산기(1990$\sim$2003)의 세 시기로 구분하여 분화와 탈분화의 양상을 살펴보았다.

• 지역연구
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• 제34권3호
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• pp.43-54
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• 2018

본 논문에서는 고령화의 진행이 초래하는 산업집적 패턴의 변화에 대해 신경제지리학적 접근을 통하여 분석하였다. 은퇴인구 변수의 추가는 단기적으로 지역별 물가, 소득, 명목임금 및 실질임금에 영향을 미친다. 일반적으로 장기균형 조건으로 채택되는 지역 간 실질임금의 균등화는 은퇴인구를 고려하지 못하므로 간접효용의 균등 조건을 추가하였으며, 새로운 장기균형을 달성케 하는 최적 세액을 제시한다. 은퇴인구의 발생은 모든 지역의 실질임금을 하락시킴과 동시에 효용기준을 만족시키는 특정 지역 제조업 종사자 비율의 지점을 하락시켜 제조업의 집적을 방해하는 반면, 세금은 은퇴인구가 발생한 지역의 효용과 실질임금을 높이고 다른 지역의 효용과 실질임금을 낮춘다. 이러한 상황에서 지역 간 실질임금 및 간접효용 비율이 같아지는 제조업 인구의 비율과 세금의 조합이 존재한다. 정부가 정액세의 부과를 통해 실질임금을 적절히 조정한다면 모든 지역에서 실질임금의 급격한 하락을 막으면서 효용수준을 안정시킬 수 있다.

• 유통과학연구
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• 제13권3호
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• pp.61-67
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• 2015

Purpose - Exports have long been regarded as significant drivers of sustainable competitive advantage and growth among small and medium enterprises (SMEs). The export activities of SMEs are particularly important in the context of export-oriented economies such as Korea. Although many studies have examined the determinants of exports, it is difficult to find empirical studies about the determinants of the export performance of regional SMEs. This study investigates the determinants of export performance in the regional SME context based on an integrated approach that combines the environment factor of industrial organization theory, competitive strategy theory, and the competences of the resource-based view. Research design, data, and methodology - To empirically analyze the determinants of export performance in the regional SMEs, data were collected from firms in the Daegu metropolitan area. Data were collected directly through questionnaire surveys; in addition, secondary financial data were also taken from the KIS-VALUE database. Out of the 175 responses that were received, 143 were considered to be worth examining. After testing the reliability and validity of the variables through multiple items such as environmental turbulence and competitive strategy, hypotheses were verified by using five multi-regression models. These models were: a control model with organizational size and age, an environmental model with technology and market turbulence, a competency model with R&D and foreign distribution channels, a strategy model with product and market differentiation, and an integrated model including all of these variables. Results - First, as a control variable, the organization size has significant positive effects on export performance. Second, technology turbulence based on industrial organization theory has significant positive effects on export performance, but market turbulence does not affect export performance. Third, the foreign market distribution competency of the resource-based view has strong positive effects on export performance, but the R&D competency does not affect export performance. Fourth, the product differentiation strategy from competitive strategy theory positively impacts export performance, but market differentiation does not affect export performance. Finally, in the integrated model, only the foreign distribution competency of the resource-based view has a significant effect on export performance. Conclusions - The empirical results of this study verified the usefulness of the rationales behind the three theories to explain the export performance of the regional SMEs, especially the importance of the foreign market distribution competency from the resource-based view. With regard to practical considerations, this study's implications suggest that the use of technological environmental changes by industries is better than the use of market changes. Further, the use of the product differentiation strategy is more effective than the use of the market-driving strategy, and the distribution channel competency plays a stronger role than the technology-oriented competency with regard to the export performance position of regional SMEs. Future studies should examine relational perspectives, such as trust among channel partners. Therefore, the configuration approach is more useful in enhancing pragmatism by comparing high- and low-export companies.

• 디자인학연구
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• 제19권5호
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• pp.351-362
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• 2006

현대 대도심 지하철은 밀폐된 공간으로 노선에 따라 다수의 역이 연결되어 있어 길찾기의 문제의 발생 빈도가 높은 것으로 알려져 있다. 따라서 이용자가 자신의 위치를 인지할 수 있도록 장소적 정보를 최대한 표현하는 시각적 장소지시단서(visual cue)가 제공되어야 할 필요성이 제기되고 있다. 본 연구는 대중교통수단으로서의 지하철 사용이 늘어감에 따라 길찾기 관련 문제들에 대한 관심이 증가하는 상황에서 서울, 동경, 타이페이 주요 지하철역의 장소지시적 단서로서 역할하는 환경디자인 사례 및 사용된 디자인 기법을 조사 분석함으로써 이용자를 위한 길찾기 지원 디자인 가이드라인 도출에 기여하는 목적을 가진다. 이론연구 결과, 시각적 장소지시단서의 유형으로 유도표식, 구역차별화, 랜드마크의 방법이 도출되었고 사례조사를 통하여 유도표식에서 노선색선의 상하배열, 양면배열, 변형 그래픽 기법이, 구역차별화에서는 벽면과 바닥패턴, 기둥과, 벽면의 강조색, 마감 재료 사용에 의한 차별화 기법이, 그리고 랜드마크에서는 수퍼그래픽, 상징물, 예술장식, 장식조명 등에 의한 기법들이 도출되었다. 서울 조사대상역의 경우, 연결노선의 색을 이용한 유도 표식이 가장 적극적으로 사용되고 있었으며 바닥패턴이나 강조색을 이용한 벽면과 기둥에 의한 구역차별화도 적극 도입하고 있었다. 또한 호돌이, 훈민정음과 같은 장소의 이해를 돕는 랜드마크적 요소도 적극 활용되고 있었다. 동경 조사대상역의 경우, 유도표식에 의존하기 보다는 구역 차별화의 방법을 적극 활용하는 것으로 나타났는데 이는 공간구조와 지시내용의 복잡성 때문인 것으로 해석된다. 타이페이시 조사대상역의 경우, 전반적으로 단순하며 통일된 이미지로서 장소지시는 최소한의 표식선이나 랜드마크에 의존하고 있었다. 특히 구역차별화의 사례가 미약하였는데 이는 대부분 역이 광장형의 오픈 스페이스로서 개방된 시야가 확보되기 때문인 것으로 해석된다. 본 연구의 결과는 향후 다양한 검증을 통하여 보다 정확한 길찾기 지원적 측면의 환경디자인 가이드라인 제시로 이어져야 할 것이다.

• 생명과학회지
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• 제29권4호
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• pp.447-454
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• 2019

비만은 에너지의 불균형으로 인하여 체내 지방조직에 지방이 축적되는 대사성질환으로 심혈관계 질환, 고혈압, 2형 당뇨, 고지혈증 및 각종 암의 발생 빈도를 증가시키는 요인이다. 지방의 축적은 지방전구세포가 지방세포로 분화하는 과정을 의미하는 adipogenesis라는 과정을 거쳐서 일어난다. 지방세포로의 분화는 다양한 호르몬과 전사인자들의 상호작용에 의해서 일어난다. 본 연구에서는 팔각회향이 항비만 소재로 활용 가능한지 확인하기 위해, 팔각회향 물 추출물을 분획하여 지방축적 억제 활성이 좋은 dichloromethane층을 선정하였다. 3T3-L1 지방전구세포가 성숙한 지방세포로 분화할 때 팔각회향 dichloromethane 층이 어떠한 기전으로 분화를 조절하는지 확인한 결과, 지방세포 분화에서 중요한 전사인자인 C/EBP family, $PPAR{\gamma}$의 발현이 억제되었고, 지방세포 최종 분화 마커로 알려져 있는 FAS 및 LPL의 발현 또한 감소되었다. 또한 G1기에서의 세포주기 정지를 통해 지방세포 분화 단계에서 필수적인 mitotic clonal expansion 단계를 억제한다는 결과를 얻었다. 이러한 연구 결과는 팔각회향이 항비만 효과를 가지는 천연물 소재로의 활용가능성을 보여주는 기초 자료가 될 것으로 사료된다.

• 농촌계획
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• 제21권1호
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• pp.129-141
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• 2015

This study classifies the types of spatio-functional differentiation in Korean island areas and analyses typical characters and suggests the development directions by each type. Eup/Myeon-level island areas are classified as six types by the factor analysis and the cluster analysis. First type is the traditional rural center. This type puts emphasis on maintaining phase as the central space and has to maximize development potential of the whole of settlement zone. Second type is the specialized region in manufacturing industry and the qualitative mutual growth of regional industries is able to be suggested. Third type is the specialized region in the neighborhood service provision. This type needs to devise the plan for utilizing potential customers actively and developing into the region specialized in tourism industry. Fourth type is the specialized region in tourism-support service functions. This type has to promote differentiated policies for maintaining amenity infra or value of countryside capital and preservation and utilization of resources by regional features. Fifth type is the fishing industry-dominated region. This type has to promote sustainable fishery development through the policy reflecting regional features and condition. Finally, sixth type is the sluggish region dominated with the traditional agriculture and fishery. This type is needed to aim at developing into the new food production base having the advantage of clean environment by strengthening support in specialized agro-fishery products. The existing researches on spatio-functional differentiation were mostly discussed with respect to land development, but this study highlights the difference in deal with the island areas distinguished from the condition of industry.

• World Technopolis Review
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• 제3권2호
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• pp.66-77
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• 2014

This paper aims at exploring a conceptual framework of analyzing the evolutionary processes of regional economies by reconciling the notion of regional resilience and the concepts prevailing in the disciplines of evolutionary economics and geography. The resilience framework appears to offer a promising outlet with which combination of the seemingly contradictory conceptions is made possible. It can address why some regions manage to adapt to external shocks, renew themselves, or lock out themselves, while others are more locked in decline. In addition, it can also explain how the spatial organization of economic production, distribution, and consumption is transformed over time. Then, regional economic resilience, together with its accompanying vehicle of panarchy, emerges as a workable framework of explaining regional differentiation in regional economic performance and trajectories. Despite the risk of being a fuzzy concept, the resilience conception can be properly operationalized to provide policy principles of regional economic innovation adjusted to region-specific contexts.

• 생약학회지
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• 제46권4호
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• pp.295-302
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• 2015

Cynanchi wilfordii Radix roots have been utilized as traditional medicine for variety of diseases including diabetes mellitus, aging progression and scavenging free radicals, enhancing immunity, reducing high serum cholesterol, and anti-tumor activity. However, the mechanisms underlying this effect remain poorly understood. The principal objective of this study was to determine the effect of cynandione A on osteoclast cells. Thus, we was isolated cynandione A from Cynanchi wilfordii Radix roots and evaluated the effect of cynandione A on receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. We found that cynandione A significantly inhibited osteoclast differentiation stimulated-RANKL in RAW 264.7 cells. Cynandione A conspicuously inhibited the mRNA and protein expression of matrix metallopeptidase 9 (MMP-9), tartrate-resistant acid phosphatase (TRAP) in cynandione A treated with RANKL. Taken together, our results demonstrated that Cynanchi Wilfordii Radix may be useful treatment option of bone-related disease such as osteoporosis leads to fracture of bone and rheumatoid arthritis.

• Nutrition Research and Practice
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• 제16권1호
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• pp.14-32
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• 2022

BACKGROUND/OBJECTIVES: Peroxisome proliferator-activated receptor-gamma co-activator-1α (PGC-1α) has a central role in regulating muscle differentiation and mitochondrial metabolism. PGC-1α stimulates muscle growth and muscle fiber remodeling, concomitantly regulating lactate and lipid metabolism and promoting oxidative metabolism. Gynostemma pentaphyllum (Thumb.) has been widely employed as a traditional herbal medicine and possesses antioxidant, anti-obesity, anti-inflammatory, hypolipemic, hypoglycemic, and anticancer properties. We investigated whether G. pentaphyllum extract (GPE) and its active compound, gypenoside L (GL), affect muscle differentiation and mitochondrial metabolism via activation of the PGC-1α pathway in murine C2C12 myoblast cells. MATERIALS/METHODS: C2C12 cells were treated with GPE and GL, and quantitative reverse transcription polymerase chain reaction and western blot were used to analyze the mRNA and protein expression levels. Myh1 was determined using immunocytochemistry. Mitochondrial reactive oxygen species generation was measured using the 2'7'-dichlorofluorescein diacetate assay. RESULTS: GPE and GL promoted the differentiation of myoblasts into myotubes and elevated mRNA and protein expression levels of Myh1 (type IIx). GPE and GL also significantly increased the mRNA expression levels of the PGC-1α gene (Ppargc1a), lactate metabolism-regulatory genes (Esrra and Mct1), adipocyte-browning gene fibronectin type III domain-containing 5 gene (Fndc5), glycogen synthase gene (Gys), and lipid metabolism gene carnitine palmitoyltransferase 1b gene (Cpt1b). Moreover, GPE and GL induced the phosphorylation of AMP-activated protein kinase, p38, sirtuin1, and deacetylated PGC-1α. We also observed that treatment with GPE and GL significantly stimulated the expression of genes associated with the anti-oxidative stress response, such as Ucp2, Ucp3, Nrf2, and Sod2. CONCLUSIONS: The results indicated that GPE and GL enhance exercise performance by promoting myotube differentiation and mitochondrial metabolism through the upregulation of PGC-1α in C2C12 skeletal muscle.