Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Suppressed Cell Proliferation and Differentiation Following an Over-expression of Myostatin is Associated with Inhibited Expression of Insulin-like Growth Factor II and Myogenin in Rat L6 Myoblasts

  • Jin, Eun-Jung (Regional Animal Industry Research Center, Jinju National University) ;
  • Kim, Inae (Regional Animal Industry Research Center, Jinju National University) ;
  • Lee, C. Young (Regional Animal Industry Research Center, Jinju National University) ;
  • Park, Byung-Chul (Nutrabio, Inc.)
  • Received : 2006.05.03
  • Accepted : 2006.06.21
  • Published : 2006.10.01
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Abstract

Myostatin (MSTN) and insulin-like growth factors (IGFs) are a known inhibitor and stimulators of proliferation and differentiation of muscle cells, respectively. The present study was performed to investigate the relationship of MSTN-induced growth inhibition to expression of the IGF system components and myogenin, a muscle cell-specific transcription factor, in rat L6 myoblasts. The L6 cells transfected with a green fluorescent protein-MSTN plasmid expression construct had a 47% less cell number than mock-transfected cells after 3-d serum-free culture, accompanied by delayed differentiation which was suggested by inhibited aggregation of cells. Moreover, cells transfected with the expression construct had decreased expression of IGF-II and myogenin genes, but not IGF-I or its receptor genes, as examined by reverse transcription-polymerase chain reaction. The reduced mitosis of the L6 cells transfected with the MSTN-expression construct increased following an addition of either IGF-I or IGF-II to the culture medium, but not to the level of mock-transfected cells. By contrast, myogenin gene expression in these cells increased after the addition of either IGF to the level of mock-transfected cells. Collectively, these results suggest that the inhibitory effect of MSTN on L6 cell proliferation and differentiation is likely to be partly mediated by serially suppressed expression of IGF-II and myogenin genes, not IGF-I gene.

Keywords

References

  1. Bach, L. A., R. Salemi and K. S. Leeding. 1995. Roles of insulinlike growth factor (IGF) receptors and IGF-binding proteins in IGF-II-induced proliferation and differentiation of L6A1 rat myoblasts. Endocrinol. 136:5061-5069 https://doi.org/10.1210/en.136.11.5061
  2. Bellinge, R. H., D. A. Liberles, S. P. Iaschi, P. A. O'brien and G. K. Tay. 2005. Myostatin and its implications on animal breeding: a review. Anim. Genet. 36:1-6 https://doi.org/10.1111/j.1365-2052.2004.01229.x
  3. Ewton, D. Z., S. L. Falen and J. R. Florini. 1987. The type II insulin-like growth factor (IGF) receptor has low affinity for IGF-I analogs: pleiotypic actions of IGFs on myoblasts are apparently mediated by the type I receptor. Endocrinol. 120:115-123 https://doi.org/10.1210/endo-120-1-115
  4. Ewton, D. Z., S. L. Falen, K. A. Magri, F. J. McWade and J. R. Florini. 1994. IGF-II is more potent than IGF-I in stimulating L6A1 myogenesis: greater mitogenic actions of IGF-I delay differentiation. J. Cell. Physiol. 161:277-284 https://doi.org/10.1002/jcp.1041610212
  5. Ewton, D. Z. and J. R. Florini. 1995. IGF binding proteins-4, -5 and -6 may play specialized roles during L6 myoblast proliferation and differentiation. J. Endocrinol. 144:539-553 https://doi.org/10.1677/joe.0.1440539
  6. Florini, J. R. 1987. Hormonal control of muscle growth. Muscle Nerve 10:577-598 https://doi.org/10.1002/mus.880100702
  7. Florini, J. R. and D. Z. Ewton. 1990. Highly specific inhibition of IGF-I-stimulated differentiation by an anti-sense oligodeoxyribonucleotide to myogenin mRNA. No effects on other actions of IGF-T. J. Biol. Chem. 265:13435-13437
  8. Florini, J. R., D. Z. Ewton and S. A. Coolican. 1996. Growth hormone and the insulin-like growth factor system in myogenesis. Endocr. Rev. 17:481-517
  9. Florini, J. R., D. Z. Ewton and S. L. Roof. 1991a. Insulin-like growth factor-I stimulates terminal myogenic differentiation by induction of myogenin gene expression. Mol. Endocrinol. 5:718-724 https://doi.org/10.1210/mend-5-5-718
  10. Florini, J. R. and K. A. Magri. 1989. Effects of growth factors on myogenic differentiation. Am. J. Physiol. 256:C701-C711 https://doi.org/10.1152/ajpcell.1989.256.4.C701
  11. Florini, J. R., K. A. Magri, D. Z. Ewton, P. L. James, K. Grindstaff and P. S. Rotwein. 1991b. 'Spontaneous' differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. J. Biol. Chem. 266:15917-15923
  12. Hwa, V., Y. Oh and R. G. Rosenfeld. 1999. The insulin-like growth factor-binding protein superfamily. Endocr. Rev. 20:761-787 https://doi.org/10.1210/er.20.6.761
  13. Jones, J. I. and D. R. Clemmons. 1995. Insulin-like growth factors and their binding proteins: biological actions. Endocr. Rev. 16:3-34
  14. Langley, B., M. Thomas, A. Bishop, M. Sharma, S. Gilmour and R. Kambadur. 2002. Myostatin inhibits myoblast differentiation by down-regulating MyoD expression. J. Biol. Chem. 277:49831-49840 https://doi.org/10.1074/jbc.M204291200
  15. Lee, C. Y., I. Kwak, C. S. Chung, W. S. Choi, R. C. M. Simmen and F. A. Simmen. 2001. Molecular cloning of the porcine acid-labile subunit (ALS) of the insulin-like growth factorbinding protein complex and detection of ALS gene expression in hepatic and non-hepatic tissues. J. Mol. Endocrinol. 26:135- 144 https://doi.org/10.1677/jme.0.0260135
  16. Lee, S. J. 2004. Regulation of muscle mass by myostatin. Annu. Rev. Cell. Dev. Biol.20:61-86 https://doi.org/10.1146/annurev.cellbio.20.012103.135836
  17. Lee, S. J. and A. C. McPherron. 1999. Myostatin and the control of skeletal muscle mass. Curr. Opin. Genet. Dev. 9:604-607 https://doi.org/10.1016/S0959-437X(99)00004-0
  18. Mangiacapra, F. J., S. L. Roof, D. Z. Ewton and J. R. Florini. 1992. Paradoxical decrease in myf-5 messenger RNA levels during induction of myogenic differentiation by insulin-like growth factors. Mol. Endocrinol. 6:2038-2044 https://doi.org/10.1210/me.6.12.2038
  19. McPherron, A. C. and S. J. Lee. 1997. Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94:12457-12461
  20. Nissley, S. P. and M. M. Rechler. 1984. Insulin-like growth factors: biosynthesis, receptors, and carrier proteins. In: Hormonal Proteins and Peptides (Ed. C. H. Lee) pp. 12:127- 201. Academic Press, New York, USA
  21. Pirottin, D., L. Grobet, A. Adamantidis, F. Farnir, C. Herens, H. D. Schroder and M. Georges. 2005. Transgenic engineering of male-specific muscular hypertrophy. Proc. Natl. Acad. Sci. USA 102:6413-6418
  22. Rechler, M. M. and D. R. Clemmons. 1998. Regulatory actions of insulin-like growth factor-binding proteins. Trends Endocrinol. Metab. 9:176-183 https://doi.org/10.1016/S1043-2760(98)00047-2
  23. Xi, G., E. Kamanga-Sollo, M. S. Pampusch, M. E. White, M. R. Hathaway and W. R. Dayton. 2004. Effect of recombinant porcine IGFBP-3 on IGF-I and long-R3- IGF-I-stimulated proliferation and differentiation of L6 myogenic cells. J. Cell. Physiol. 200:387-94 https://doi.org/10.1002/jcp.20068
  24. Yaffe, D. 1968. Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc. Natl. Acad. Sci. USA 61:477-483
  25. Yang, W., Y. Zhang, G. Ma, X. Zhao, Y. Chen and D. Zhu. 2005. Identification of gene expression modifications in myostatinstimulated myoblasts. Biochem. Biophys. Res. Commun. 326:660-666 https://doi.org/10.1016/j.bbrc.2004.11.096

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