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Effect of Cynandione A of Cynanchi Wilfordii Radix in RANKL and Lipopolysaccharide-induced on Osteoclastogeneis in RAW 264.7 Cells  

Hwang, Joon-Ho (Biotechnology Regional Innovation Center, Jeju National University)
Yi, Mi-Ran (Biotechnology Regional Innovation Center, Jeju National University)
Kang, Chang-Hee (Biotechnology Regional Innovation Center, Jeju National University)
Bu, Hee-Jung (Biotechnology Regional Innovation Center, Jeju National University)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.4, 2015 , pp. 295-302 More about this Journal
Abstract
Cynanchi wilfordii Radix roots have been utilized as traditional medicine for variety of diseases including diabetes mellitus, aging progression and scavenging free radicals, enhancing immunity, reducing high serum cholesterol, and anti-tumor activity. However, the mechanisms underlying this effect remain poorly understood. The principal objective of this study was to determine the effect of cynandione A on osteoclast cells. Thus, we was isolated cynandione A from Cynanchi wilfordii Radix roots and evaluated the effect of cynandione A on receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. We found that cynandione A significantly inhibited osteoclast differentiation stimulated-RANKL in RAW 264.7 cells. Cynandione A conspicuously inhibited the mRNA and protein expression of matrix metallopeptidase 9 (MMP-9), tartrate-resistant acid phosphatase (TRAP) in cynandione A treated with RANKL. Taken together, our results demonstrated that Cynanchi Wilfordii Radix may be useful treatment option of bone-related disease such as osteoporosis leads to fracture of bone and rheumatoid arthritis.
Keywords
Cynanchi Wilfordii Radix; Cynandione A; Osteoclast; RANKL; LPS;
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