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Effect of the Disinfectants on Microbes Changes in Piggery Slurry (가축방역용 소독제 처리가 액비내 미생물 변화에 미치는 영향)

  • Choi Dong-Yoon;Suh Guk-Hyun;Kwang Jung-Hoon;Park Chi-Ho;Jeong Kwang-Hwa;Kim Tae-Il;Yang Chang-Bum;Cho Yong-Il;Choi Hong-Lim
    • Journal of Animal Environmental Science
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    • v.11 no.3
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    • pp.161-168
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    • 2005
  • The disinfectants are important to prevention of infectious diseases for livestock and have been used widespreadly in recent years. This study was conducted to determine the effect of the disinfectants on microorganisms with reference to disinfectants concentration and application period in piggery slurry. As material, chemically different four disinfectants were used and no disinfectant, as control. These four disinfectants were Acids, Basic, Aldehydes and Alkalis, respectively. The characteristics pH, moisture content and organic matter of the piggery slurry used in this study were 8.2, $98.6\%,\;0.4\%$, respectively. The initial total microorganisms of piggery slurry were $6.3\times10^5$ cfu/ml, and Escherichia coli, Staphylococcus aureus, Bacillus cereus were $1.2\times10^4,\;4.1\times10^4,\;1.7\times10^4 cfu/ml,$ respectively. The disinfectants were applied at $100\%,\;200\%\;and\;300\%$ concentrations on the piggery slurry surface. It was determined that the effect of disinfectants varied accordance to concentration and application time. The most sensitive microorganisms were Escherichia coli and Staphylococcus aureus, while Bacillus cereus was found to be durable. As a result of this study, the microorganinsms of the piggery slurry are on the decrease by all disinfectants during 2 weeks, but showed a tendency to increase in number after that time. Accordingly, the microorganisms which are concerned in the liquid composting of piggery slurry were not affected by the disinfectant after 2 weeks.

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Development and Characterization of EMS-induced Mutants with Enhanced Salt Tolerance in Silage Maize (EMS 유도 내염성 증진 사료용 옥수수 돌연변이체 선발 및 특성 분석)

  • Cho, Chuloh;Kim, Kyung Hwa;Seo, Mi-Suk;Choi, Man-Soo;Chun, Jaebuhm;Jin, Mina;Kim, Dool-Yi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.406-415
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    • 2020
  • Maize (Zea mays L.) is one of the most valuable agricultural crops and is grown under a wide spectrum of environmental conditions. However, maize is moderately sensitive to salt stress, and soil salinity is a serious threat to its production worldwide. In this study, we used ethyl methane sulfonate (EMS) to generate salt-tolerant silage maize mutants. We screened salt-tolerant lines from 203 M3 mutant populations by evaluating the morphological phenotype after salt stress treatment and selected the 140ES91 line. The 140ES91 mutant showed improved plant growth as well as higher proline content and leaf photosynthetic capacity compared with those of wild-type plants under salt stress conditions. Using whole-genome re-sequencing analysis, 1,103 single nucleotide polymorphisms and 71 insertions or deletions were identified as common variants between KS140 and 140ES91 in comparison with the reference genome B73. Furthermore, the expression patterns of three genes, which are involved in salt stress responses, were increased in the 140ES91 mutant under salt stress. Taken together, the mutant line identified in our study could be used as an improved breeding material for transferring salt tolerance traits in maize varieties.

Establishment of Biotin Analysis by LC-MS/MS Method in Infant Milk Formulas (LC-MS/MS를 이용한 조제유류 중 비오틴 함량 분석법 연구)

  • Shin, Yong Woon;Lee, Hwa Jung;Ham, Hyeon Suk;Shin, Sung Cheol;Kang, Yoon Jung;Hwang, Kyung Mi;Kwon, Yong Kwan;Seo, Il Won;Oh, Jae Myoung;Koo, Yong Eui
    • Journal of Food Hygiene and Safety
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    • v.31 no.5
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    • pp.327-334
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    • 2016
  • This study was conducted to establish the standard method for the contents of biotin in milk formulas. To optimize the method, we compared several conditions for liquid extraction, purification and instrumental measurement using spiked samples and certified reference material (NIST SRM 1849a) as test materials. LC-MS/MS method for biotin was established using $C_{18}$ column and binary gradient 0.1% formic acid/acetonitrile, 0.1% formic acid/water mobile phase is applied for biotin. Product-ion traces at m/z 245.1 ${\rightarrow}$ 227.1, 166.1 are used for quantitative analysis of biotin. The linearity was over $R^2=0.999$ in range of $5{\sim}60{\mu}g/L$. For purification, chloroform was used as a solvent for eliminating lipids in milk formula. The linearity was over 0.999 in range of 5~60 ng/mL. The detection limit and quantification limit were 0.10, 0.31 ng/mL. The accuracy and precision of LC-MS/MS method using CRM were 103%, 2.5% respectively. Optimized methods were applied in sample analysis to verify the reliability. All the tested milk formulas were acceptable contents of biotin compared with component specification and standards for nutrition labeling. The standard operating procedures were prepared for biotin to provide experimental information and to strengthen the management of nutrient in milk formula.

Diameters of the Thoracic Aorta Measured with Multidetector Computed Tomography (다중검출 전산화 단층촬영을 이용하여 측정한 흉부대동맥의 직경)

  • Lee, Gun;Lim, Chang-Young;Lee, Hyeon-Jae
    • Journal of Chest Surgery
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    • v.42 no.1
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    • pp.79-86
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    • 2009
  • Background: Background: Computed tomography (CT) is the main tool for detecting abnormalities of the thoracic aorta, but conventional CT only shows the cross-sectional images. These CT images have some limitations fo accuratly measuring the thoracic aortic diameters at various levels. Multidetector computed tomography (MDCT) overcomes these limitations. We measured the thoracic aortic diameter perpendicular to the loop-shaped thoracic aortic course and this was studied in relation to age, gender, height, weight, the body surface area, the body mass index and the presence of hypertension. Material and Method: Thirty hundred thirty one patients (males: 141 patients and females: 190 patients) who had no abnormalities of the thoracic aorta were investigated using MDCT aortography. They were divided into three age categories: 20~39 years old, 40~59 years old and over age 60. The image was reformed with multiplanar reconstruction and the diameter of the aorta was measured perpendicular to the aortic course at 5 anatomic segments. Level A was the mid-ascending aorta, level B was the distal ascending aorta, level C was the aortic arch, level D was the aortic isthmus and level E was the mid-descending aorta. Result: The mean age was 49.5 years old for males and 54.9 years old for females (p<0.05). The mean diameter of the thoracic aorta at level A was 31.1 mm, that at level B was 30.2 mm, that at level C was 26.5 mm, that at level D was 24.0 mm and that at level E was 22.6 mm. The diameters at all the levels were gradually increased with age. Hypertensive patients had larger diameters than did the non-hypertensive population. There was a positive correlation between the ascending aortic diameter (levels A&B) and height and the body surface area, but there were no statistical differences at the aortic arch (level C) and the descending aorta (levels D&E). There were no statistical differences of the weight and body mass index at all levels. Conclusion: The diameters of the thoracic aortas were directly correlated with gender, age and hypertension. Height and the body surface area were only correlated with the ascending aorta. Weight and the body mass index have no statistical difference at all levels. We measured the age related thoracic aortic diameters and the upper normal limits and we provide this data as reference values for the thoracic aortic diameter in the Korean population.

A Study on Development of Management Targets and Evaluation of Target Achievement for Non-point Source Pollution Management in Saemangeum Watershed (새만금 비점오염원 관리지역에서의 목표설정 및 달성도 평가방법론 연구)

  • Kim, Eun-Jung;Park, Bae-Kyung;Kim, Yong-Seok;Rhew, Doug-Hee;Jung, Kwang-Wook
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.8
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    • pp.480-491
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    • 2015
  • In this study, methods using LDC (Load Duration Curve) and watershed model were suggested to develope management targets and evaluate target achievement for non-point source pollution management considering watershed and runoff characteristics and possibility for achievement of target. These methods were applied for Saemangeum watershed which was designated as nonpoint source pollution management area recently. Flow duration interval of 5 to 40% was selected as flow range for management considering runoff characteristics and TP was selected as indicator for management. Management targets were developed based on scenarios for non-point source pollutant reduction of management priority areas using LDC method and HSPF model which was calibrated using 4 years data (2009~2012). In the scenario of LID, road sweeping and 50% reduction in CSOs and untreated sewage at Jeonju A20 and 30% reduction in fertilizer and 50% in livestock NPS at Mankyung C03, Dongjin A14 and KobuA14, management targets for Mangyung bridge, Dongjin bridge, Jeonju stream and Gunpo bridge were developed as TP 0.38, 0.18, 0.64 and 0.16 mg/L respectively. When TP loads at the target stations were assumed to have been reduced by a certain percentage (10%), management targets for those target stations were developed as TP 0.35, 0.17, 0.60 and 0.15 mg/L respectively. The result of this study is expected to be used as reference material for management master plan, implementation plan and implementation assessment for non-point source management area.

A Rapid Analysis of 226Ra in Raw Materials and By-Products Using Gamma-ray Spectrometry (감마분광분석을 이용한 원료물질 및 공정부산물 중 226Ra 신속분석방법)

  • Lim, Chung-Sup;Chung, Kun-Ho;Kim, Chang-Jong;Ji, Young-Yong
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.15 no.1
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    • pp.35-44
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    • 2017
  • A gamma-ray peak of $^{226}Ra$ (186.2 keV) overlaps with one of $^{235}U$ (185.7 keV) in a gamma-ray spectrometry system. Though reference peaks of $^{235}U$ can be used to correct the peak interference of $^{235}U$ in the analysis of $^{226}Ra$, this requires a complicated calculation process and a high limit of quantitation. On the other hand, evaluating $^{226}Ra$ using the correction constant in the overlapped peak can make a rapid measurement of $^{226}Ra$ without the complicated calculation process as well as overcome the disadvantage in the indirect measurement of $^{214}Bi$, which means the confinement of $^{222}Rn$ gas in a sample container and a time period to recover the secular equilibrium. About 93 samples with 6 species for raw-materials and by-products were prepared to evaluate the activity of $^{226}Ra$ using the correction constant. The results were compared with the activity of $^{214}Bi$, which means the indirect measurement of $^{226}Ra$, to validate the method of the direct measurement of $^{226}Ra$ using the correction constant. The difference between the direct and indirect measurement of $^{226}Ra$ was generally below about ${\pm}20%$. However, in the case of the phospho gypsum, a large error of about 50% was found in the comparison results, which indicates the disequilibrium between $^{238}U$ and $^{226}Ra$ in the materials. Application results of the contribution ratio of $^{226}Ra$ were below about ${\pm}10%$. The direct measurement of $^{226}Ra$ using the correction constant can be an effective method for its rapid measurement of raw materials and by-products because the activity of $^{226}Ra$ can be produced with a simple calculation without the consideration of the integrity of a sample container and the time period to recover the secular equilibrium.

A Study on the Comparative Art theory in the Arts and Crafts Movement and Post-impressionism (미술 공예 운동과 후기 인상주의 비교 예술론 연구)

  • 박연실
    • Archives of design research
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    • v.20
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    • pp.279-291
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    • 1997
  • The history of modern design begins with the arts and crafts movement(1860). The importance of the movement which decorated the outset gave birth to all the trends of thought which would occur under the circumstances within it, and is deemed that the ideas of the figures who played an active part in the movement might be ceaselessly continued through the works and ideas of their outstanding juniors or purpils as a doctrine of the philosophy of design. Therefore, it might be a prerequisite that the significance and spirit of the movement, and its developing process should be addressed in detail in the first place, but for the limited space of this paper, it was intended to desvribe only the part which can be interpreted in duplicate, linked with post-impressionism. The subject of this thesis is about a theory of art in which both ideas of the movement(1860) and the post-impressionism(1910) are comparatively reviewed. The genre, strictly speaking, is classified into the history of painting, and there is a gap fo about an half century between the issues which are comparatively discussed here. Both the movements began in a same environmental place of England, specially the movement at Milieu in England, and since there is a common point that the representative runners of each movement, William Morries(1834-1896) and Roger Fry(1866-1934), belong to a same race of Engol-Saxon, their ideas coincide with an aesthetic scholar, H. Tanie's aesthetic interpretation method and the more important is, as being elucidated in the comment and aesthetic theory for which Roger Fry gave effort and activity in his later life, that when he read intensively Ruskin's books, $\ulcorner$Modern Painters$\lrcorner$and$\ulcorner$Stones of Venice$\lrcorner$he had solidified his idea of post-impressionism while giving approval and criticism on them. After all, as in a co-painter, Windyham Lewis's reference of 'Roger Fry's Too Late Morris Movement', he, inspired by the actual activities of Morris, played activities similar to that of Morris in which exhibiting and selling some of his decorative art works signed by him and the works of post-impressionism through (1913-1920). Herein, that is wished to add a remark by this author is a point that the author of$\ulcorner$Vision and Design$\lrcorner$, Roger Fry, has not be made a subject of discussion specially in the Korean world of design. So, with this case of a thesis, it's wished that many latent awakened, design persons in korea give efforts to researching into Roger Fry so that their findings could be officially announced in the would. By the way, what is tried to describe in this paper from now on is to analyse and review the origin of post-impressionism which idea was first coined in the art world through the 1st and 2nd exhibitions of 'Manet and Post-impressionism' which were opened each at the Grafton Gallery in 1910 and 1913 by him. And also, it is intended to review it through the art journals and some references by critics of the day in which favorable criticism or severe criticism were ready to comment through the opinions and influences of the coworkers of Roger Fry, say, Clive Bell, Desmond Maccarthy, etc. and of himself as a main axis, on the art ideas of Gauguin, Gogh, Matisse, and Cezanne whose works were the typical ones participated and exhibited in those 1st and 2nd exhibitions.

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Study of Iodide Adsorption on Organobentonite using X-ray Absorption Spectroscopy (X-선 흡수분광기를 이용한 유기벤토나이트의 요오드 흡착연구)

  • Yoon, Ji-Hae;Ha, Ju-Young;Hwang, Jin-Yeon;Hwang, Byoung-Hoon;Gordon E. Brown, Jr.
    • Journal of the Mineralogical Society of Korea
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    • v.22 no.1
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    • pp.23-34
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    • 2009
  • The adsorption of iodide on untreated bentonite and bentonites modified with organic cation (i.e., hexadecylpyridinium chloride monohydrate ($HDP^+$)) was investigated, and the organobentonites were characterized using uptake measurements, ${\mu}$-XRD, and electrophoretic mobilities measurement. Uptake measurements indicate that bentonite has a high affinity for $HDP^+$. Our ${\mu}$-XRD study indicates that organobentonites significantly expanded in basal spacing and organic cations were substantially intercalated into the interlayer spaces of bentonite. The electrophoretic mobility indicates that organobentonite tht is modified with organic cations in excess of the CEC of bentonite is completely different from untreated bentonite in the surface charge distribution. We found significant differences in adsorption capacities of iodide depending on the bentonite properties as follows: iodide adsorption capacities were 439 mmol/kg for the bentonite modified with $HDP^+$ at an equivalent amount corresponding to 200% of the CEC of bentonite whereas no adsorption of iodide was observed for the untreated bentonite. The molecular environments of iodine adsorbed on organobentonites were further studied using I K-edge and $L_{III}$-edge x-ray absorption spectroscopy (XAS). The X-ray absorption near-edge structure (XANES) of iodine spectra from organobentonites was similar to that of KI reference solution. Linear combination fitting of EXAFS data suggests the fraction of iodine reacted with the organic compound increased with increasing loading of the organic compound on organobentonites. In this study, we observed significant differences in the adsorption environments of iodide depending on the modified property of bentonite and suggest that an organobentonite has potential as reactive barrier material around a nuclear waste repository containing anionic radioactive iodide.

Development of 'the safety' theme-based integrated teaching·learning process plans for the middle school Home Economics Instruction (중학교 가정과 수업을 위한 '안전' 주제 중심 통합 교수·학습 과정안 개발)

  • Kim, Nam Eun;Chae, Jung Hyun;Cho, Jae Soon
    • Journal of Korean Home Economics Education Association
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    • v.28 no.1
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    • pp.19-39
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    • 2016
  • The purpose of this study was to develop the safety theme-based integrated teaching learning process plans for the middle school Home Economics(HE) Instruction and ultimately to contribute for the middle school students to live their safe lives. To achieve the goals of this study, HE curriculum documents from the 1st to the 2015 revision were analyzed and a survey was conducted to identify the middle school students' current status of safety accidents and needs on the HE safety education. The respondents of the survey were the 512 students of one ${\bigcirc}{\bigcirc}$ Girls' Middle School in Busan. And then, the seven integrated themes were selected, each seven integrated theme-networks were formed, and safety theme-based Home Economics curriculum and learning materials were developed. The results of this study were as the follows. The safety education content has continually been included from the first HE curriculum of 1954 to the 2015 revised HE curriculum. The middle school student respondents highly needed the content of 'the methods to deal with sexual violence and prostitution prevention', 'suicide prevention', and 'bullying net' for the HE safety education. All the 42 items were needed for the HE safety education by the respondents. The safety theme-based HE teaching learning process plans developed finally included the seven integrated themes, which were (1) dietary life safety, (2) adolescents' sex and safety, (3) adolescents' relationships with friends and safety, (4) family life and safety, (5) dwelling life and safety, (6) adolescents' egos and safety, and (7) social environment and safety. Each integrated theme consisted of three to six small themes, which amounted to total 28(for 35 lessons). Each small theme was presented with learning objectives and particular goals. The total 157 learning materials including the Home Economics curriculum were developed, which offered learning objectives and content for each safety theme, total 28 teaching and learning plans(for 35 lessons) were developed, which offered specific instructions for the easy implementation of the curriculum in the classroom, 28 PPTs, 25 film materials, four reading materials, 61 workbooks, 14 activity sheets, 16 evaluation sheets, 3 test sheets, 2 reference materials, and 4 learning material models(the refrigerator model, traffic lights for discussions, food tray model and stickers, and food mileage card). In this study, the safety education themes of 'clothing life and safety', 'conflict and safety', 'professional life and safety', and 'consumer and safety' were not addressed because these theme were not needed highly by the respondents. Therefore, for the further development of the teaching learning process plans for the HE safety education, it is necessary to develop and evaluate the teaching learning process plans to address the themes of 'clothing life and safety', 'conflict and safety', 'professional life and safety', and 'consumer and safety'.

Anti-aging Effect of Inula britannica var. chinensis Flower Extract According to the Extraction Temperature (추출 온도에 따른 금불초 꽃 추출물의 항노화 효능)

  • Jeon, Ji Min;Yoo, Dae Sung;Cheon, Jong Woo;Kwon, Soon Sik;Jeon, So Ha;Park, Soo Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.1
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    • pp.109-120
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    • 2014
  • In this study, the extracts of Inula britannica var. chinensis (I. britannica) flower were extracted at three different temperatures (room temperature, $45^{\circ}C$, and $65^{\circ}C$) and their anti-aging effects were studied. Before investigating anti-aging effects of the extracts, their cytotoxicity was tested on B16F10, Hs683, and HaCaT cells. All extracts showed no cytotoxicity at the concentration less than 0.1% (v/v). Melanin synthesis inhibitory activities in B16F10 cells and reverse transcriptase polymerase chain reaction (RT-PCR) in Hs683 and HaCaT cells were used to see their anti-aging effects. The room temperature extract at 0.1% showed 24.5% melanin synthesis inhibition, which was better than the $45^{\circ}C$ and $65^{\circ}C$ extracts. In addition, expression rates of the room temperature extract at 0.1% on HAS-1, HAS-2, and HAS-3 related to hyaluronan synthase genes were 123.3%, 137.8%, 133.2%, respectively. which were higher than reference material of L-ascorbic acid. Expression rates of the $45^{\circ}C$ extract at 0.1% on TNF-${\alpha}$, COX-2, and IL-$1{\alpha}$, which are inflammatory related genes, was suppressed to 30.3%, 12.8%, 25.7%, respectively. It was better in anti-in flammatory effect than the room temperature and $65^{\circ}C$ extracts. As results, we showed that I. britannica var. chinensis flower extarcts decreased melanin production and expression of inflammatory related genes and increased the expression rate of hyaluronan synthase genes. Thus, it is believed that the extracts affect anti-aging effects of skin through whitening, moisturizing, and anti-inflammatory processes and could be applicable to cosmetics as a functional cosmetic ingredient.