• Molecules and Cells
• /
• v.42 no.5
• /
• pp.418-425
• /
• 2019

Multicistronic elements, such as the internal ribosome entry site (IRES) and 2A-like cleavage sequence, serve crucial roles in the eukaryotic ectopic expression of exogenous genes. For utilization of multicistronic elements, the cleavage efficiency and order of elements in multicistronic vectors have been investigated; however, the dynamics of multicistronic element-mediated expression remains unclear. Here, we investigated the dynamics of encephalomyocarditis virus (EMCV) IRES- and porcine teschovirus-1 2A (p2A)-mediated expression. By utilizing real-time fluorescent imaging at a minute-level resolution, we monitored the expression of fluorescent reporters bridged by either EMCV IRES or p2A in two independent cultured cell lines, HEK293 and Neuro2a. We observed significant correlations for the two fluorescent reporters in both multicistronic elements, with a higher correlation coefficient for p2A in HEK293 but similar coefficients for IRES-mediated expression and p2A-mediated expression in Neuro2a. We further analyzed the causal relationship of multicistronic elements by convergent cross mapping (CCM). CCM revealed that in all four conditions examined, the expression of the preceding gene causally affected the dynamics of the subsequent gene. As with the cross correlation, the predictive skill of p2A was higher than that of IRES in HEK293, while the predictive skills of the two multicistronic elements were indistinguishable in Neuro2a. To summarize, we report a significant temporal correlation in both EMCV IRES- and p2A-mediated expression based on the simple bicistronic vector and real-time fluorescent monitoring. The current system also provides a valuable platform to examine the dynamic aspects of expression mediated by diverse multicistronic elements under various physiological conditions.

• International Journal of Advanced Culture Technology
• /
• v.8 no.3
• /
• pp.223-230
• /
• 2020

Companies have made an investment of cost and time to optimize processing of a new business model in a cloud environment, applying collaboration technology utilizing business processes in a social network. The collaborative processing method changed from traditional BPM to the cloud and a mobile cloud environment. We proposed a collaborative system for operating processes in social networks using MOXMDR-DAI+ (eXtended Metadata Registry-Data Access & Integration based multimedia ontology). The system operating cloud-based collaborative processes in application of MOXMDR-DAI+, which was suitable for data interoperation. MOXMDR-DAI+ applied to this system was an agent effectively supporting access and integration between multimedia content metadata schema and instance, which were necessary for data interoperation, of individual local system in the cloud environment, operating collaborative processes in the social network. In operating the social network-based collaborative processes, there occurred heterogeneousness such as schema structure and semantic collision due to queries in the processes and unit conversion between instances. It aimed to solve the occurrence of heterogeneousness in the process of metadata mapping using MOXMDR-DAI+ in the system. The system proposed in this study can visualize business processes. And it makes it easier to operate the collaboration process through mobile support. Real-time status monitoring of the operation process is possible through the dashboard, and it is possible to perform a collaborative process through expert search using a community in a social network environment.

• Journal of the Korean Society for Precision Engineering
• /
• v.26 no.7
• /
• pp.65-72
• /
• 2009

Laser interferometry is widely used as a measuring system in many fields because of its high resolution and its ability to measure a broad area in real-time all at once. In conventional laser interferometry, for example out-of-plane ESPI (Electronic Speckle Pattern Interferometry), in plane ESPI, shearography and holography, it uses PZT or other components as a phase shift instrumentation to extract 3-D deformation data, vibration mode and others. However, in most cases PZT has some disadvantages, which include nonlinear errors and limited time of use. In the present study, a new type of laser interferometry using a laser diode is proposed. Using Laser Diode Sinusoidal Phase Modulating (LD-SPM) interferometry, the phase modulation can be directly modulated by controlling the laser diode injection current thereby eliminating the need for PZT and its components. This makes the interferometry more compact. This paper reports on a new approach to the LD (Laser Diode) Modulating interferometry that involves four-frame phase shift method. This study proposes a four-frame phase mapping algorithm, which was developed to have a guaranteed application, to stabilize the system in the field and to be a user-friendly GUI. In this paper, the theory for LD wavelength modulation and sinusoidal phase modulation of LD modulating interferometry is shown. Using modulating laser and research of measurement algorithm does comparison with existent ESPI measurement algorithm. Algorithm measures using GPIB communication through most LabVIEW 8.2. GPIB communication does alteration through PC. Transformation of measurement object measures through modulating laser algorithm that develops. Comparison of algorithm of modulating laser developed newly with existent PZT algorithm compares transformation price through 3-D. Comparison of 4-frame phase mapping, unwrapping, 3-D is then introduced.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.28 no.6
• /
• pp.1489-1497
• /
• 2018

Web application services have diversified. At the same time, research on intrusion detection is continuing due to the surge of cyber threats. Also, As a single-defense system evolves into multi-level security, we are responding to specific intrusions by correlating security events that have become vast. However, it is difficult to check the OS, service, web application type and version of the target system in real time, and intrusion detection events occurring in network-based security devices can not confirm vulnerability of the target system and success of the attack A blind spot can occur for threats that are not analyzed for problems and associativity. In this paper, we propose the validation of effectiveness for intrusion detection events using TF-IDF. The proposed scheme extracts the response traffics by mapping the response of the target system corresponding to the attack. Then, Response traffics are divided into lines and weights each line with an TF-IDF weight. we checked the valid intrusion detection events by sequentially examining the lines with high weights.

• Journal of Institute of Control, Robotics and Systems
• /
• v.5 no.2
• /
• pp.189-199
• /
• 1999

This paper presents an optimization algorithm for a stable Self Dynamic Neural Network(SDNN) using genetic algorithm. Optimized SDNN is applied to a problem of controlling nonlinear dynamical systems. SDNN is dynamic mapping and is better suited for dynamical systems than static forward neural network. The real-time implementation is very important, and thus the neuro controller also needs to be designed such that it converges with a relatively small number of training cycles. SDW has considerably fewer weights than DNN. Since there is no interlink among the hidden layer. The object of proposed algorithm is that the number of self dynamic neuron node and the gradient of activation functions are simultaneously optimized by genetic algorithms. To guarantee convergence, an analytic method based on the Lyapunov function is used to find a stable learning for the SDNN. The ability and effectiveness of identifying and controlling a nonlinear dynamic system using the proposed optimized SDNN considering stability is demonstrated by case studies.

• The KIPS Transactions:PartA
• /
• v.13A no.4 s.101
• /
• pp.371-380
• /
• 2006

This paper utilizing model picture of finished clothes in fashion design field various material (textile fabrics) doing Draping directly can invent new design, and do not produce direction sample or poetic theme width and confirm clothes work to simulation. Also, construct database about model and material image and embodied system that can confirm Mapping result by real time. And propose clothes simulation system to dress to 3D human body model of imagination because using several cloth pieces first by process to do so that can do simulation dressing abstracted poetic theme width to 3D model here. Proposed system creates 3D model who put clothes by physical simulation that do fetters to mass-spring model after read 3D human body model file and 2D foundation pattern file. System of this treatise examines collision between triangle that compose human body model for realistic simulation and triangle that compose clothes and achieved reaction processing. Because number of triangle to compose human body is very much, this collision examination and reaction processing need much times. To solve this problem, treatise that see could create realistic picture by method to diminish collision public prosecutor and reaction processing number, and could dress clothes to imagination human body model within water plant taking advantage of Octree space sharing techniques.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.18 no.10
• /
• pp.63-68
• /
• 2017

Although automated metro subway systems have the advantage of operating a train without a train driver, it is difficult to detect an immediate fault condition and take countermeasures when an unusual situation occurs. Therefore, it is important to construct a maintenance information system (MIS) that detects the vehicle failure/status information in real time and maintains it efficiently in the depot of the railway's vehicles. This paper proposes a conceptual design method that realizes the interface between the train control system (TCS), the operation control center train control monitoring system (OCC-TCMS) console, and the MIS using wireless communication network in real-time. To transmit a large amount of information on 800,000 occurrences per day during operation, data was collected in a 56 byte data table using a data processing algorithm. This state information was classified into 4 hexadecimal codes and transmitted to the MIS by mapping the status and the fault information on the vehicle during the main line operation. Furthermore, the transmission and reception data were examined in real time between the TCS and MIS, and the implementation of the failure information screen was then displayed.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.31 no.8A
• /
• pp.820-829
• /
• 2006

IP-based design methodology has been popularly employed for SoC design to reduce design complexity and to cope with time-to-market pressure. Due to the request for high performance of current mobile systems, embedded SoC design needs a multi-processor to manage problems of high complexity and the data processing such as multimedia, DMB and image processing in real time. Interface module for communication between system buses and processors are required, since many IPs employ different protocols. High performance processors require interface module to minimize the latency of data transmission during read-write operation and to enhance the performance of a top level system. This paper proposes an automatic interface generation system based on FSM generated from the common protocol description sequence of a bus and an IP. The proposed interface does not use a buffer which stores data temporally causing the data transmission latency. Experimental results show that the area of the interface circuits generated by the proposed system is reduced by 48.5% on the average, when comparing to buffer-based interface circuits. Data transmission latency is reduced by 59.1% for single data transfer and by 13.3% for burst mode data transfer. By using the proposed system, it becomes possible to generate a high performance interface circuit automatically.

• Proceedings of the KIEE Conference
• /
• 1987.07b
• /
• pp.1276-1279
• /
• 1987

Cardiac arrhythmias are associated with electrical Instability and, hence, with abnormal mechanical activity of the heart in many cases, arrhythmias can be treated with drugs or electric shock to control and/or stop them. Hence fast arrhythmia detection is very important. In this paper RR interval, QRS width, and morphology are used for diagnosis and QRS complex is detected by hardware system. hence diagnosing time is shorten. Moreover doctors or nurses who have little knowledge of computer manipulation can get the Information of Patient's ECG by showing characteristics of abnormal waveform and by mapping graphs of RR interval .vs. QRS width and RR interval .vs. morphology on screen.

• Journal of Korea Spatial Information System Society
• /
• v.11 no.1
• /
• pp.195-199
• /
• 2009

Augmented reality is a field of computer research which deals with the combination of real-world and computer-generated data, where computer graphics objects are blended into real footage in real time and it has tremendous potential in visualizing geospatial information. However, to utilize augmented reality in mobile system, many researches have undergone with GPS or marker based approaches. Localization and tracking of current position become more complex problem when it is used in indoor environments. Many proposed RF based tracking and localization. However, it does cause deployment problems of large sensors and readers. In this paper, we present a noble markerless AR approach for indoor navigation system only using a camera. We will apply this work to mobile seamless indoor/outdoor u-GIS system.