• Title/Summary/Keyword: QTL분석

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Mapping of QTL based on molecular map conferring agronomic traits in recombinant inbred lines from tonsil type X Japonica type of rice (Oryza sativa L.) I. Days to heading, culm length, leaf blast (벼의 통일형과 일반형 교배후대에서 분자유전자 지도를 이용한 주요 농업형질의 양적형질 유전자좌(QTL) 분석 I. 출수일수, 간장, 잎도열병)

  • Kang Hyeon Jung;Cho Yong Gu;Lee Young Tae;Eun Moo Young;Shim Jai Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1996.10a
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    • pp.24-25
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    • 1996
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Mapping of QTL based on molecular map conferring agronomic traits in recombinant inbred lines from tongil type X Japonica type of rice(Oryza sativa L.) III. Grain shape (벼의 통일형과 일반형 교배후대에서 분자유전자 지도를 이용한 주요 농업형질의 양적형질 유전자좌(QTL) 분석 III. 종실의 형태적 특성)

  • Kang Hyeon Jung;Cho Yong Gu;Lee Young Tae;Eun Moo Young;Suk Soon Jong;Shim Jai Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1996.10a
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    • pp.28-29
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    • 1996
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Mapping of QTL based on molecular map conferring agronomic traits in recombinant inbred lines from tongil type X Japonica type of rice(Oryza sativa L.) IV. White core, white belly and alkali digestion value (벼의 통일형과 일반형 교배후대에서 분자유전자 지도를 이용한 주요 농업형질의 양적형질 유전자좌(QTL) 분석 IV. 심백, 복백 및 알칼리 붕괴도)

  • Kang Hyeon Jung;Cho Yong Gu;Lee Young Tae;Eun Moo Young;Cho Soo Yeon;Shim Jai Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1996.10a
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    • pp.30-31
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    • 1996
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Mapping of QTL based on molecular map conferring agronomic traits in recombinant inbred lines from tonsil type X Japonica type of rice(Oryza sativa L.) II. Yield and yield components (벼의 통일형과 일반형 교배후대에서 분자유전자 지도를 이용한 주요 농업형질의 양적형질 유전자좌(QTL) 분석 II. 수량 및 수량구성요소)

  • Kang Hyeon Jung;Cho Yong Gu;Lee Young Tae;Lee Seung Yeob;Eun Moo Young;Shim Jai Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1996.10a
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    • pp.26-27
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    • 1996
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Mapping Grain Weight QTL using Near Isogenic Lines from an Interspecific Cross (벼 종간잡종 유래 근동질 유전자계통 이용 종자중 관여 유전자 분석)

  • Kang, Ju-Won;Yang, Paul;Yun, Yeo-Tae;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.43 no.4
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    • pp.304-310
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    • 2011
  • In previous studies, we reported QTLs for grain weight (GW), qGW3 and for spikelets per panicle (SPP), qSPP3 linked to RM60 on chromosome 3 using advanced backcross lines derived from a cross between Oryza sativa ssp. Indica cv. Milyang 23 and O. glaberrima. The O. glaberrima alleles at this locus increased GW and spikelets per panicle in the Milyang 23 background. To further confirm and narrow down the position of the QTLs on chromosome 3, substitution mapping was performed using five lines containing the target O. glaberrima segment on chromosome 3. The size and position of the O. glaberrima segment on chromosome 3 were different in each line. These lines possessed 3-10 non-target O. glaberrima introgressions in the Milyang 23 background. These five lines were evaluated for seven agronomic traits including 1,000 grain weight and spikelets per panicle and also genotyped with seven SSR markers. Four lines were informative in delimiting the position of QTLs, qGW3 and qSPP3. Two lines with the O. glaberrima segment flanked by SSR markers, RM60 and RM523 displayed significantly higher values than Milyang 23 in GW and SPP whereas two lines without that O. glaberrima segment displayed no difference in GW and SPP compared to Milyang 23. The result indicates that two QTL, qGW3 and qSPP3 are located in the interval between RM60 and RM523 which are 1.2-Mb apart. Introgression lines having QTLs, qGW3 and qSPP3 would be useful materials not only to indentify the relationship between these two yield QTLs, but also to develop high yielding variety via marker-aided selection technology.

Analysis of Quantitative Trait Loci (QTLs) for Unsaturated Fatty Acid Contents in Soybean Seed Using Recombinant Inbred Lines (콩에서 microsatellite marker를 이용한 불포화지방산 함량의 양적형질 유전자좌의 분석)

  • Kim, Hyeun-Kyeung;Im, Moo-Hyeog;Choung, Myoung-Gun
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1665-1670
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    • 2008
  • Soybean oil is an important source of vegetable oil for human food and nonfood applications and accounts for approximately 22% of the world's total edible oil production. Improvement of the quality and quantity of soybean seed oil constituents is one of the most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control oleic, linoleic, and linolenic acid contents in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.85, 0.82 and 0.81, respectively. Eight independent QTLs for oleic acid content were identified from linkage group (LG) A2, C1, D2, F, G, L, and O. Seven QTLs for linoleic acid content were located on LG D1b, E, H, I and L. Oil content was related with five QTLs located on LG C1, H, J, K, and L. Oleic, linoleic, and linolenic acid have two common QTLs on LG C1 and L. Thus, we identified major loci improving soybean oil quality.

Mapping and Race Specific Reaction of the Resistance Gene Pi45(t) in Rice (벼 도열병 저항성 유전자 Pi45(t)의 균계 특이적 반응과 고밀도지도 작성)

  • Kim, Dong-Min;Ju, Hong-Guang;Yang, Paul;Han, Seong-Sook;Roh, Jae-Hwan;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.42-49
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    • 2011
  • QTL analysis for blast resistance was carried out using 140 $BC_3F_3$ lines derived from a cross between Ilpum as a recurrent parent and Moroberekan as a donor parent. 140 $BC_3F_3$ lines with the parents were inoculated with nine blast isolates. To identify QTLs for resistance to nine blast isolates, 134 SSR markers showing polymorphisms between the parents were genotyped for the 140 $BC_3F_3$ lines. A total of 17 resistance QTLs to nine isolates were detected on chromosomes 2, 3, 4, 6, 7, 9 and 10. The phenotypic variance explained by each QTL ranged from 8.2% to 26.4%. The Moroberekan alleles contributed the positive effect at these 17 QTL loci. In a previous study, the QTL, Pi45(t) for durable resistance to blast was identified using a sequential planting method. To know the relationship between Pi45(t) and the isolate-specific resistance gene, an $F_2$ population was developed from a cross between Ilpum and an introgression line harboring Pi45(t). $F_3$ lines segregating for the Pi45(t) were inoculated to three isolates. $F_3$ lines from the $F_2$ plants with the Moroberekan segment at the target region showed resistance to two isolates. This result seems to indicate that the Pi45(t) and the isolate-specific resistance gene are tightly linked or the resistance is controlled by the same gene(s). The markers linked to genes controlling blast resistance would be useful in developing blast resistance lines in the breeding program.