• 한국식품과학회지
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• 제36권4호
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• pp.598-603
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• 2004

산지가 다른 지역(대만, 중국, 한국, 미국)에서 자란 4종의 메밀전분의 이화학적 특성을 살펴보았다. 그 결과를 살펴보면 전분의 외형은 4종 모두 다각형의 입자로 넓게 분포되어 있으며, X-ray 관찰결과 대다수의 천연 전분과 마찬가지로 4종 모두 A형에 속해 있었다. 수분함량은 6.3-9.58%사이에 있었으며, 조 단백은 대만산과 중국산이 한국산과 미국산시료보다 높게 나타났으며, 지방함량은 한국산과 중국산이 다른 2종의 시료보다 높게 나타냈다. 청가(BV)를 살펴보면 중국산이 0.39로 가장 높게 나타났고, 한국산이 0.32로 가장 낮게 나타났다. 아밀로오스 함량은 미국산이 27.6으로 가장 높고, 한국산이 가장 낮았다. 물결합력에서는 대만산의 시료가 가장 높고, 미국산이 가장 낮았다. 아밀로오스 함량이 비교적 높은 중국산과 미국산은 팽윤력과 용해도에서 낮게 나타났다. 신속점도측정기(RVA)에 의한 측정결과 최고점도, 가공의 안정성을 나타내는 breakdown, 노화에 밀접한 관계를 나타내는 setback 모두 미국산이 가장 높게 나타나 미국산이 비교적 hard gel한 경향을 나타내었다.

• 한국포장학회지
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• 제12권1호
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• pp.55-61
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• 2006

'Bing' 체리 (P. avium L.)는 짧은 저장성을 가지는 신선 농산물로, 코팅에 의한 저장성 향상 가능성을 알아보았다. 네 종류 ($Semperfresh^{(R)}$, calcium caseinate, chitosan, or TIC $Pretested^{(R)}$ colloid 911)의 식이성 코팅 재료를 1% 농도로 체리에 코팅 처리 한 후 $2^{\circ}C$, 88% 상대습도 조건에서 35일 간 저장하며 코팅 물질에 따른 저장성 및 품질변화를 확인하였다. $Semperfresh^{(R)}$ 코팅은 체리의 전반적인 품질 및 저장성 향상에 기여하였는데, 무게 감소를 줄이고 표면 색변화를 억제하는 것으로 관측되었다. 키토산을 기본으로 하는 코팅은 저장 중 곰팡이 발생을 억제하는데 효과적이었다. 하지만 colloid 911 과 calcium caseinate를 이용한 코팅은 신선 체리의 저장성 향상에 기여하지 못했는데, 이들 친수성 코팅 물질의 경우, 체리의 표피층과 코팅 물질 사이의 상호작용에 의해서 체리의 자연적인 보호층인 큐티클 층이 영향을 받음으로 체리의 무게 감소가 저장 기간 중에 촉진되었던 것으로 사료된다.

• Journal of Nutrition and Health
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• 제36권9호
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• pp.933-941
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• 2003

It has been suggested that green juice supplementation may have some health promoting benefits. We evaluated the effects of green juice (Angelica keiskei) consumption on parameters of lipid profiles and plasma antioxidant status in healthy male smokers. Fifty-four smokers were supplemented with 300 ml of green juice for 6 weeks while maintaining their normal diet. Blood samples were collected on week 0 and week 6 in order to evaluate plasma lipid profiles (total cholesterol, triglyceride, LDL-cholesterol, HDL-cholesterol) , plasma antioxidant vitamin levels (ascorbic acid, $\alpha$ -tocopherol, ${\gamma}$ -tocopherol, $\alpha$ -carotene, $\beta$ -carotene, cryptoxanthin and lycopene) , the degree of LDL oxidation and GOT, GPT levels for liver function. Plasma ascorbic acid level remained at the same level. However, $\alpha$ -tocopherol and ${\gamma}$ -tocopherol normalized by total cholesterol (p <0.05) and $\beta$ -carotene (p <0.001) level were all significantly increased after green juice supplementation. Plasma cholesterol was reduced for 12%, LDL-cholesterol was reduced for 9.3% after green juice consumption, while plasma triglyceride and HDL-cholesterol was not changed. Oxidized LDL assessed by conjugated diene (CD) , was decreased (p < 0.0001) after green juice consumption. These results further support a role for green juice supplementation in the improvement of lipid status, prevention of lipid peroxidation, and thereby reducing risk factors of numerous diseases associated with elevated oxidative stress in smokers.

• Nutrition Research and Practice
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• 제9권1호
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• pp.49-56
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• 2015

BACKGROUND/OBJECTIVES: Glutathione S-transferase (GST) forms a multigene family of phase II detoxification enzymes which are involved in the detoxification of reactive oxygen species. This study examines whether daily supplementation of kale juice can modulate blood pressure (BP), levels of lipid profiles, and blood glucose, and whether this modulation could be affected by the GSTM1 and GSTT1 polymorphisms. SUBJECTS/METHODS: 84 subclinical hypertensive patients showing systolic BP over 130 mmHg or diastolic BP over 85 mmHg received 300 ml/day of kale juice for 6 weeks, and blood samples were collected on 0-week and 6-week in order to evaluate plasma lipid profiles (total cholesterol, triglyceride, HDL-cholesterol, and LDL-cholesterol) and blood glucose. RESULTS: Systolic and diastolic blood pressure was significantly decreased in all patients regardless of their GSTM1 or GSTT1 polymorphisms after kale juice supplementation. Blood glucose level was decreased only in the GSTM1-present genotype, and plasma lipid profiles showed no difference in both the GSTM1-null and GSTM1-present genotypes. In the case of GSTT1, on the other hand, plasma HDL-C was increased and LDL-C was decreased only in the GSTT1-present type, while blood glucose was decreased only in the GSTT1-null genotype. CONCLUSIONS: These findings suggest that the supplementation of kale juice affected blood pressure, lipid profiles, and blood glucose in subclinical hypertensive patients depending on their GST genetic polymorphisms, and the improvement of lipid profiles was mainly greater in the GSTT1-present genotype and the decrease of blood glucose was greater in the GSTM1-present or GSTT1-null genotypes.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1142-1149
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• 2004

Streptococcus mutans has the capacity of inducing dental caries. Thus, to develop a novel way of preventing dental caries, a cell wall hydrolase-producing strain was isolated and its characteristics were investigated. Among 200 alkalophilic strains isolated from soil, 8 strains exhibited lytic activities against Streptococcus mutans. However, strain YU5215 with the highest cell wall hydrolase activity was selected for further study. Strain YU5215 was identified as a novel strain of Bacillus based on analyzing its 16S rDNA sequence and Bergey's Manual of Systematic Bacteriology, and thus designated as Bacillus mutanolyticus YU5215. The optimal conditions for the production of the cell wall hydrolase from Bacillus mutanolyticus YU5215 consisted of glucose ($0.8\%$), yeast extract ($1.2\%$), polypeptone ($0.5\%$), $K_{2}HPO_{4}\;(0.1\%$), $MgSO_{4}{\cdot}7H_{2}O$ ($0.02\%$), and $Na_{2}CO_{3}\;(1.0\%$) at pH 10.0. Bacillus mutanolyticus YU5215 was cultured at 30^{circ}C for 72 h to produce the cell wall hydrolase, which was then purified by acetone precipitation and CM-agarose column chromatography. The molecular weight of the lytic enzyme was determined as 22,700 Da by SDS-PAGE. When the cell wall peptidoglycan of Streptococcus mutans was digested with the lytic enzyme, no increase in the reducing sugars was observed, while the free amino acids increased, indicating that the lytic enzyme had an endopeptidase-like property. The amino terminus of the cell wall peptidoglycan digested by the lytic enzyme was determined as a glutamic acid, while the lytic site of the lytic enzyme in the Streptococcus mutans peptidoglycan was identified as the peptide linkage of L-Ala and D-Glu.

• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1051-1058
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• 2014

Platelet-activating factor receptor (PAFR) plays an important role in bacterial infection and inflammation. We examined the effect of the bacterial cell wall components lipopolysaccharide (LPS) and lipoteichoic acid (LTA) from Lactobacillus plantarum (pLTA) and Staphylococcus aureus (aLTA) on PAFR expression in THP-1, a monocyte-like cell line. LPS and aLTA, but not pLTA, significantly increased PAFR expression, whereas priming with pLTA inhibited LPS-mediated or aLTA-mediated PAFR expression. Expression of Toll-like receptor (TLR) 2 and 4, and CD14 increased with LPS and aLTA treatments, but was inhibited by pLTA pretreatment. Neutralizing antibodies against TLR2, TLR4, and CD14 showed that these receptors were important in LPS-mediated or aLTA-mediated PAFR expression. PAFR expression is mainly regulated by the nuclear factor kappa B signaling pathway. Blocking PAF binding to PAFR using a PAFR inhibitor indicated that LPS-mediated or aLTA-mediated PAF expression affected TNF-${\alpha}$ production. In the mouse small intestine, pLTA inhibited PAFR, TLR2, and TLR4 expression that was induced by heat-labile toxin. Our data suggested that pLTA has an anti-inflammatory effect by inhibiting the expression of PAFR that was induced by pathogenic ligands.

• Nutrition Research and Practice
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• 제6권1호
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• pp.9-15
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• 2012

This study was performed to examine the feeding effects of $Angelica$ $keiskei$ $Koidz$ (AK) and its processed products on serum, liver, and body fat content and the expression of antioxidant genes in rats fed a high fat diet. AK and its processed products were added at 3-5% to a high fat diet and fed to adult rats for 6 weeks. In experiment 1 (EXP 1), the rats were fed with one of six diets including a control diet (normal fat), high fat diet (HF), and HF + AK additives groups (four groups). In experiment 2 (EXP 2), the rats were separated into three groups of HF, HF + AK whole leaves, and HF + fermented juice (FS) + squeeze (SA). Body weight was not different among the groups in either experiment. The liver weight was lower in the FS and SA groups compared to that in the other groups (P<0.05). Serum luteolin was higher in the AK and processed products groups compared to that in the HF group (P<0.05). Gene expression of the antioxidative enzymes catalase and glutathione-s-reductase in the liver was higher in the AK processed products group than that in the other groups (P<0.05). The results suggest that the intake of AK and its processed products increased the expression of antioxidant enzymes in animals fed a high fat diet, reduced hepatic cholesterol content, and increased the effective absorption of luteolin.

• 한국유통학회지:유통연구
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• 제16권3호
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• pp.105-133
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• 2011

본 연구는 친환경농산물 구매에 있어서 소비자들이 생각하는 가격변수의 중요도를 살펴보고 가격수준에 대한 소비자의 의견에 영향을 미치는 요인들을 분석하는데 그 목적이 있다. 연구의 결과를 보면, 직접질문 결과 현재 친환경농산물 구매에 있어서 소비자들은 '제품에 대한 상세한 설명', '유통채널', '친환경식품 표기에 대한 신뢰' 등을 가격보다 더 중요하게 생각하고 있는 것으로 나타났다. 다음으로 서열로짓분석 결과가 제시하는 바는 '어린 자녀의 수'나 '가족 중 환자 유무' 등 상황적 요인이 친환경농산물의 가격수준에 대한 소비자들의 의견에 가장 큰 영향을 미치는 변수로 나타났다. 그리고 마지막으로 컨조인트분석 결과를 보면 '유통채널'이 가장 중요한 속성으로 나타났으며, 다음으로 '표기에 대한 신뢰' 그리고 가격 순으로 나타났다. 이는 첫 번째 직접질문방식의 결과가 제시하는 바와 비슷한 결과로 풀이된다. 즉 '친절한 설명'이라는 변수는 컨조인트분석에 포함하지 않았음을 감안할 때, 주어진 상품프로파일 상의 선택을 이용한 간접적인 분석 결과와 직접적으로 질문한 결과가 같은 의미를 제공하고 있음을 알 수 있다.

• 한국식품조리과학회지
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• 제21권5호
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• pp.662-668
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• 2005

To investigate the quality retention method of salted and semi-dried mackerel fillets, pH, volatile basic nitrogen (VBN), trimethylamine (TMA) and total bacterial counts in the fillets that were UV treated for 1, 3, and 4 hr were periodically measured during 48 days of refrigerated storage. The moisture, crude protein, crude lipid, and ash contents were 45.46 %, 18.87 %, 33.75 %, and 2.99 %, respectively. The freshness as determined by pH measurement could be maintained up to 25 days for the control and 31 days for the UV-treated fillets. VBN contents were continually increased over the storage time. The control reached the initial putrid level after 21 days while the UV-treated fillets after $25{\sim}31$ days. A similar tendency was shown for the changes of trimethylamine TMA contents, although the period differed slightly and the increase of the UV treatment time could decrease the TMA contents. The changes on the total bacterial counts of the fillets were maintained under 105 CFU/g during 48days. Especially, due to the effective decrease of the microorganism count by UV treatment at the beginning of the storage, UV -treated fillets showed lower bacterial counts than control over the whole period. E. coli. and Listeria were not detected in any fillets. Combining the above data and the sensory changes, the storage period of the fillets could be prolonged from 15 days at present to 30 days by UV treatments.

• 대한지역사회영양학회지
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• 제10권2호
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• pp.183-188
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• 2005

The aim of this study was to assess the effect of 6-week vegetable juice supplementation (360 ml/day) on serum lipid profiles and antioxidant activity in college women. Twenty women (mean age: 21) with normal life style and dietary pattern and who are free of any specific diseases were recruited among the student in S women's university. The subjects consumed vegetable juice to take part in an uncontrolled clinical trial for a 6-week intervention period. While there was no difference in the concentration of total cholesterol and HDL-cholesterol, serum concentrations of TG and HDL/LDL ratio were significantly reduced by $22\%$ (p = 0.013) and $6\%$ (p =0.007) respectively. Significant decrease in manlondialdehyde (p = 0.000) was accompanied by an increase in the activity of serum antioxidant enzymes, such as GSHPx (p = 0.000), SOD (p=0.007). It was also found that total antioxidant status was improved by $5.4\%$ (p=0.009). Serum parameters were all changed without affecting body mass index, waist and hip circumferences, or nutrient intakes after six week of supplementation. This study demonstrated that the supplementation regular meals with vegetable juice can favorably affect serum lipid profiles and antioxidant systems, and hence could contribute to reduce the risks of chronic diseases in college women.