• Title/Summary/Keyword: Pre-irradiation

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Enhancement of Analyte Ionization in Desoprtion/Ionization on Porous Silicon (DIOS)-Mass Spectrometry(MS)

  • Lee Chang-Soo;Kim Eun-Mi;Lee Sang-Ho;KIm Min-Soo;Kim Yong-Kweon;Kim Byug-Gee
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.3
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    • pp.212-217
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    • 2005
  • Desorption/ionization on silicon mass spectrometry (DIOS-MS) is a relatively new laser desorption/ionization technique for mass spectrometry without employing an organic matrix. This present study was carried to survey the experimental factors to improve the efficiency of DIOS-MS through electrochemical etching condition in structure and morphological properties of the porous silicon. The porous structure of silicon structure and its properties are crucial for the better performance of DIOS-MS and they can be controlled by the suitable selection of electrochemical conditions. The fabrication of porous silicon and ion signals on DIOS-MS were examined as a function of silicon orientation, etching time, etchant, current flux, irradiation, pore size, and pore depth. We have also examined the effect of pre- and post-etching conditions for their effect on DIOS-MS. Finally, we could optimize the electrochemical conditions for the efficient performance of DIOS-MS in the analysis of small molecule such as amino acid, drug and peptides without any unknown noise or fragmentation.

Inactivation of Mycobacterium using Ultrasonic and Ultraviolet Sequential Processes (초음파와 자외선 연계공정을 이용한 Mycobacterium 불활성화)

  • Kim, Wangi;Jung, Yeonjung;Yoon, Yeojoon;Lim, Gwanhun;Kim, Jongbae;Kang, Joon-Wun
    • Journal of Korean Society on Water Environment
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    • v.28 no.1
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    • pp.57-62
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    • 2012
  • In this study, the inactivation efficiency of Mycobacterium marinum was evaluated in buffered water (pH 7) using a low pressure ultraviolet (LP-UV) lamp, ultrasonic (US), and UV/US sequential processes. In the UV alone process, 3 log inactivation of the M. marinum was achieved with a UV dose of $120mJ/cm^2$. However, a tailing phase was later observed because M. marinum has a high tendency for cell aggregation. Even though the M. marinum was not inactivated in the US alone process, the hydrophobicity decreased and turbidity increased due to the crumbling of the cell aggregation. Among the candidate processes which were UV alone, US-UV sequential process and UV-US-UV sequential process, the US-UV sequential process showed the highest synergistic effects for M. marinum inactivation. Consequently, US is a very useful process as a UV irradiation pre-treatment to inactivate M. marinum in water.

Inhibition of SMP30 Gene Expression Influences the Biological Characteristics of Human Hep G2 Cells

  • Zhang, Sheng-Chang;Liang, Ming-Kang;Huang, Guang-Lin;Jiang, Kui;Zhou, Su-Fang;Zhao, Shuang
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.3
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    • pp.1193-1196
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    • 2014
  • Senescence marker protein 30 (SMP30), a hepatocellular carcinoma (HCe) associated antigen had been identified by our research group. To study its mechanisms of regulation and associations with the occurrence and development of HCe, we inhibited expression by RNAi technique, and observed effects on the biological characteristics of Hep G2 cells. In cell viability assays, cell growth in the experimental group (with siRNA transfection) was elevated. In Transwell invasion assays, compared with blank and control groups, numbers of invading cells in the experimental group were significantly increased, whereas in apoptosis assays, the percentage apoptosis demonstrated no differences, but after UV irradiation, that in the experimental group was higher than the other two groups. In a word, SMP30 can inhibit the proliferation and invasion of human hepatoma cells and thus can be regarded as a cancer suppressive factor.

A study for prediction of temperature distribution in laser-assisted turning for rod-shaped cast iron (레이저 보조선삭 중 주철환봉 내부의 온도분포 예측에 관한 연구)

  • Kim, Kwan-Woo;Cho, Hae-Yong;Lee, Jae-Hoon;Suh, Jeong;Shin, Dong-Sig
    • Laser Solutions
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    • v.13 no.2
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    • pp.10-16
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    • 2010
  • Laser-assisted machining is dependent on absorbed energy density into workpiece. Generally, the absorptivity of laser beam is dependent on wave length of laser, materials, surface roughness, etc. Various shapes and energy densities for beam irradiation can be used to laser-assisted machining. In this thesis, efficient method of heat source modeling was developed and designed by using one fundamental experimental trials. And then, laser-assisted machining of rod-shaped cast iron was simulated by using commercial FEM code MARC. Simulations and experiments with various conditions were carried out to determine suitable condition of pre-heating for laser-assisted turning process. Temperature distribution of cutting zone could be predicted by simulation.

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Embryonic Effects of Ultrasound Irradiation on Preimplantation Stage of ICR Mouse Embryos - About embryonic death and malformation of ultrasound mechanisms - (초음파(超音波)에 대한 ICR Mouse 착상전기(着床前期)의 개체(個體) Level 영향(影響)(기형(奇形).배사망.(胚死亡))으로부터 초음파(超音波)의 물리학적(物理學的) 특성(特性)에 대한 연구(硏究))

  • Song, Jae-Kwan;Kim, Ye-Hyun
    • Journal of radiological science and technology
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    • v.18 no.2
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    • pp.75-86
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    • 1995
  • Embryos and fetuses are more sensitive to various environmental agents than adults of children biological effects following the exposure, such as intrauterin, malformation, have intimate conception with the prenatal exposure. There have been many studies on radiation and other agent. However, imformation about the ultrasound effects is limited. It is very important to study the effect of ultrasound with these kinds of fatera in consideration of ultrasound protection and safty. In this study, embryonic and fefal effects of ICR mouse embryos irradiated on 24, 48, 12 and 192 hpc of preimplantation and organogenesis period at the intensity of $0.5{\sim}3\;W/cm^2$ were investigated. Many type of external malformation observed in mouse irradiated on 72 hpc and 192 hpc. However, the embryos irradiated on 24 hpc and 48 hpc, at witch embryos had less then 6 cells and were pre-compaction stage, had no sensitivity for external malformation. The threshold doses of external malformation in mouse irradiated on 72 hpc and 192 hpc, at which embryos were consisted of $16{\sim}32$ cells and neural formation stage, were $1\;W/cm^2$ and $0.5\;W/cm^2$.

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Experimentally Minimized Contaminative Condition of Carbonaceous Artifacts in Transmission Electron Microscope (투과전자현미경에 타소질 불순물의 오염 최소화를 위한 실험 조건)

  • Kim, Young-Min;Choi, Joo-Hyoung;Song, Kyung;Kim, Yang-Soo;Kim, Youn-Joong
    • Applied Microscopy
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    • v.39 no.1
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    • pp.73-77
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    • 2009
  • Contaminative artifacts such as carbonaceous materials on carbon-coated microgrids are unavoidable, which is induced by electron beam exposure inside electron microscopes. This phenomenon raise a source to produce confusing information to the samples investigated by analytical TEM, which should be alleviated as much as possible. As experimental precautions for reducing this unwanted effect, the use of $LN_2$ cooled anti-contaminator and pre-illumination of electron beam at low magnification can be helpful. Nevertheless, we should be cautious to set an illumination condition for microanalysis because the contaminative effect is dependent with the types of irradiation situations, which is well known to be a decisive factor for causing the carbonaceous artifacts. Accordingly, it is necessary that optimal illumination to minimize the contaminative effect should be selected for improving the accuracy of microanalysis. In this paper, we introduce the practical method to determine the optimal illumination condition by evaluating the contaminative effect as a function of instrumental spot size, which is directly linked with electron current density.

A Study on Weatherability with Particle Size and Orientation of Aluminum in Pre-painted Basecoat for Automotive (자동차용 선도장 베이스코트에서 알루미늄 입자 크기와 배향에 따른 내후성 연구)

  • Lee, Jae Woo;Cho, Ur Ryong
    • Elastomers and Composites
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    • v.48 no.2
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    • pp.167-171
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    • 2013
  • Weatherability was examined according to aluminum particle size and its orientation used in PCM(Paint Coated Metal). Substrate delamination was checked by different UV transmission rate with QUV$^{(R)}$ (Q-LAB) equipment that can make accelerated UV irradiation. Film was prepared using three different types of conflake aluminum pastes under a PCM coating process and clearcoat was sprayed. UV transmission test results showed low transmission rate when EKART #790 having the smallest particle size and horizontal orientation was used. From the gloss retention results, gloss retention was highest in EKART #790 and followed by #770, #750.

Liquid Crystal Alignment on Multi-stacked Layer HfO2 Thin Films Using a Solution-process (용액 공정 기반의 다중 적층된 HfO2 박막 상에서의 액정 배향)

  • Kim, Dai-Hyun
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.26 no.11
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    • pp.821-825
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    • 2013
  • Effect of multi-stacked layer (MSL), 0.1 mol (M) and 0.3 mol (M) hafnium oxide ($HfO_2$) alignment layers were fabricated via a solution-process for LCs orientation. The solutions were spin-coated and annealed in a furnace. MSL consists of three sub-layers using 0.1 M solution, mono-layer (ML) is composed of 0.3 M $HfO_2$ solution. Then ion-beam irradiation was treated with 1.8 keV for 2 min. $HfO_2$-based LC cells were investigated through photographs, pre-tilt angle using crystal rotation method, X-ray photoelectron spectroscopy (XPS) measurement, and surface roughness using atomic force microscopy(AFM) for their characteristic research. Good LC orientation characteristics were observed on MSL $HfO_2$ surface. The LC alignment mechanism on MSL $HfO_2$ and ML $HfO_2$ surfaces was attributed to van der Waals (VDW) interaction between the LC molecular and substrate surface.

Preparation of MWCNT/TiO2 Composites by Using MWCNTs and Titanium(IV) Alkoxide Precursors in Benzene and their Photocatalytic Effect and Bactericidal Activity

  • Oh, Won-Chun;Zhang, Feng-Jun;Chen, Ming-Liang
    • Bulletin of the Korean Chemical Society
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    • v.30 no.11
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    • pp.2637-2642
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    • 2009
  • In this present paper, we prepared $MWCNT/TiO_2$ composites by using pre-oxidized multi-walled carbon nanotubes (MWCNTs) with different titanium alkoxide precursors in benzene solvent. The composites were comprehensively characterized by BET surface area, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), energy dispersive X-ray analysis (EDX) and UV-Vis absorption spectroscopy. The photoactivity of the prepared materials, under UV irradiation, was tested using methylene blue (MB) in aqueous solution. Finally, according to the results of MB removal experiment, it can be considered that the MB degradation infect mainly caused by photocatalytic effect of $TiO_2$. Furthermore, the bactericidal test of the composites was also determined. It was indicated that $MWCNT/TiO_2$ composites with sunlight had greater effectiveness for B. cereus, S. aureus and E. coli than any other experimental conditions.

Theoretical studies on the stabilization and diffusion behaviors of helium impurities in 6H-SiC by DFT calculations

  • Obaid Obaidullah;RuiXuan Zhao;XiangCao Li;ChuBin Wan;TingTing Sui;Xin Ju
    • Nuclear Engineering and Technology
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    • v.55 no.8
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    • pp.2879-2888
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    • 2023
  • In fusion environments, large scales of helium (He) atoms are produced by a radical transformation along with structural damage in structural materials, resulting in material swelling and degradation of physical properties. To understand its irradiation effects, this paper investigates the stability, electronic structure, energetics, charge density distribution, PDOS and TDOS, and diffusion processes of He impurities in 6HSiC materials. The formation energy indicates that a stable, favorable position for interstitial He is the HR site with the lowest energy of 2.40 eV. In terms of vacancy, the He atom initially prefers to substitute at pre-existing Si vacancy than C vacancy due to lower substitution energy. The minimum energy paths (MEPs) with migration energy barriers are also calculated for He impurity by interstitial and vacancy-mediated diffusion. Based on its calculated energy barriers, the most possible diffusion path includes the exchange of interstitial and vacancy sites with effective migration energies ranging from 0.101 eV to 1.0 eV. Our calculation provides a better understanding of the stabilization and diffusion behaviors of He impurities in 6H-SiC materials.