Inactivation of Mycobacterium using Ultrasonic and Ultraviolet Sequential Processes

초음파와 자외선 연계공정을 이용한 Mycobacterium 불활성화

  • Kim, Wangi (The Office of Waterworks Seoul Metropolitan Goverment) ;
  • Jung, Yeonjung (Department of Environmental Engineering, YIEST, Yonsei University) ;
  • Yoon, Yeojoon (Department of Environmental Engineering, YIEST, Yonsei University) ;
  • Lim, Gwanhun (Department of Biomedical Laboratory Science, Yonsei University) ;
  • Kim, Jongbae (Department of Biomedical Laboratory Science, Yonsei University) ;
  • Kang, Joon-Wun (Department of Environmental Engineering, YIEST, Yonsei University)
  • 김완기 (서울시 상수도사업본부) ;
  • 정연정 (연세대학교 환경공학부) ;
  • 윤여준 (연세대학교 환경공학부) ;
  • 임관훈 (연세대학교 임상병리학과) ;
  • 김종배 (연세대학교 임상병리학과) ;
  • 강준원 (연세대학교 환경공학부)
  • Published : 2012.01.30

Abstract

In this study, the inactivation efficiency of Mycobacterium marinum was evaluated in buffered water (pH 7) using a low pressure ultraviolet (LP-UV) lamp, ultrasonic (US), and UV/US sequential processes. In the UV alone process, 3 log inactivation of the M. marinum was achieved with a UV dose of $120mJ/cm^2$. However, a tailing phase was later observed because M. marinum has a high tendency for cell aggregation. Even though the M. marinum was not inactivated in the US alone process, the hydrophobicity decreased and turbidity increased due to the crumbling of the cell aggregation. Among the candidate processes which were UV alone, US-UV sequential process and UV-US-UV sequential process, the US-UV sequential process showed the highest synergistic effects for M. marinum inactivation. Consequently, US is a very useful process as a UV irradiation pre-treatment to inactivate M. marinum in water.

Keywords

References

  1. 정연정, 오병수, 강준원(2006). Bacillus Subtilis Spores 불활성화 실험을 통한 오존, UV 공정의 소독 특성 평가, 수질보전 한국물환경학회지, 22(4), pp. 672-677.
  2. Blume, T. and Neis, U. (2004). Improved Wastewater Disinfection by Ultrasonic Pre-treatment, Ultrasonics Sonochemistry, 11, pp. 333-336.
  3. Bohrerova, Z. and Linden, K. G. (2006). Ultraviolet and Chlorine Disinfection Mycobacterium in Wastewater: Effect of Aggregation, Water Environment Research, 78(6), pp. 565-571.
  4. Covert, T. C., Rodgers, M. R., Reyes, A. L., and Stelma, G. N. (1999). Occurrence of Nontuberculous Mycobacteria in Environmental Samples, Applied and Environmental Microbiology, 65(6), pp. 2492-2496.
  5. Falkinham, J. O. (2003) Factors Influencing the Chlorine Susceptibility of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum, Applied and Environmental Microbiology, 69(9), pp. 5685-5689.
  6. Falkinham, J. O., Norton, C. D., and LeChevallier, M. W. (2001). Factors Influencing Numbers of Mycobacterium avium, Mycobacterium intracllulare and Other Mycobacteria in Dringking Water Distribution Systems, Applied and Environmental Microbiology, 67(3), pp. 1225-1231.
  7. Hua, I. and Thompson, J. E. (2000). Inactivation of Escherichia coli by Sonication at Discrete Ultrasonic Frequencies, Water Research, 34(15), pp. 3888-3893.
  8. Joyce, E., Phull, S. S., Lorimer, J. P., and Mason, T. J. (2003). The Development and Evaluation of Ultrasound for the Treatment of Bacterial Suspensions. A Study of Frequency, Power and Sonication Time on Cultured Bacillus Species, Ultrasonics Sonochemistry, 10(6), pp. 315-318.
  9. Le Dantec, C., Duguet, J. P., Montiel, A., Dumoutier, N., Dubrou, S., and Vincent, V. (2002a). Chlorine Disinfection of Atypical Mycobacteria Isolated from a Water Distribution System, Applied and Environmental Microbiology, 68(3), pp. 1025-1032.
  10. Le Dantec, C., Duguet, J. P., Montiel, A., Dumoutier, N., Dubrou, S., and Vincent, V. (2002b). Occurrence of Mycobacteria in Water Treatment Lines and in Water Distribution Systems, Applied and Environmental Microbiology, 68(11), pp. 5318-5325.
  11. Lee, E. S., Lee, M. Y., Han, S. H., and Ka, J. O. (2008). Occurrence and Molecular Differentiation of Environmental Mycobacteria in Surface Waters, Journal of Microbiology and Biotechnology, 18(7), pp. 1207-1215.
  12. Mamane-Gravetz, H. and Linden, K. G. (2005). Relationship Between Physiochemical Properties Aggregation and UV Inactivation of Isolated Indigenous Spores in Water, Journal of Applied Microbiology, 98(2), pp. 351-363.
  13. Oguma, K., Katayama, H., and Ohgaki, S. (2004). Photoreactivation of Legionella pneumophila after Inactivation by Low- or Medium-pressure Ultraviolet Lamp, Water Research, 38, pp. 2757-2763.
  14. Pembrey, R. S., Marshall, K. C., and Schneider, R. P. (1999). Cell Surface Analysis Techniques: What do Cell Preparation Protocols do to Cell Surface Properties?, Applied and Environmental Microbiology, 65(7), pp. 2877-2894.
  15. Qualls, R. G., Dorfman, M. H., and Johnson, J. D. (1989) Evaluation of the Efficiency of UV Disinfection Systems, Water Research, 23, pp. 317-325.
  16. Springthorpe, S., Sander, M., Nolan, K., and Sattar, S. A. (2001). Comparison of Static and Dynamic Disinfection Models for Bacteria and Viruses in Water of Varying Quality, Water Science Technology, 43(12), pp. 147-154.
  17. Taylor, R. H., Falkinham J. O., Norton, C. D., and LeChevallier, M. W. (2000). Chlorine, Chloramine, Chlorine Dioxide, and Ozone Susceptibility of Mycobacterium avium, Applied and Environmental Microbiology, 66(4), pp. 1702-1705.
  18. United States Environmental Protection Agency (USEPA) (2003) Ultraviolet Disinfection Guidance Manual, EPA 815-D-03-007, Washington DC, US, pp. 39-52.
  19. Vantarakis, A., Tsintzou, A., Diamandopoulos, A., and Papapetropoulou, M. (1998). Non-tuberculosis Mycobacteria in Hospital Water Supplies, Water Air and Soil Pollution, 104(3-4), pp. 331-337.