• Journal of Life Science
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• 제20권11호
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• pp.1634-1639
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• 2010

This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 mg of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at $10^{\circ}C$ and $13^{\circ}C$. More than 93% of the male population was produced by this method and growth of hormone-treated fish at $13^{\circ}C$ was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least $3,600\;erg/cm^2$ was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at $28^{\circ}C$ for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.

• Journal of Life Science
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• 제18권3호
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• pp.344-349
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• 2008

Normal somatic cells proliferate for a limited number of doublings in culture and then enter an irreversible growth-arrest state called replicative senescence. Replicative senescence has been believed a reason for the limited cellular turnover and deterioration of tissue function in aged animals. However, there is no experimental evidence supporting this assumption. Furthermore, cells from aged person have been poorly characterized with an exception of the cases of T cells. In this study, we examined cell biological changes occurring in replicative senescence of fibroblast strains originated from a new-born (NHF-NB) and a 87 year old man (NHF-87). NHF-87 (and the cells from a 75-year old) proliferated to smaller population doublings and with longer doubling times than NHF-NB did. At early passages, NHF-87 exhibited a low senescence-associated ${\beta}-Gal$ (SA ${\beta}-Gal$) activity and lipofuscin level, typical markers for cellular senescence. Furthermore, they maintained low levels of lysosome and reactive oxygen species (ROS). All of these levels increased dramatically in the late passage NHF-87 quite similarly as those in the late passaged NHF-NB did. These results indicate that most cells originated from the aged maintain a phenotype of the cells originated from new-born donors and undergo replicative senescence with the same kinetics as that of the cells from new-born. It is also indicated that not SA ${\beta}-gal$ activity but cell proliferation rate may be qualified as a biomarker for cells aged in vivo.

• Korean journal of applied entomology
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• 제40권4호
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• pp.327-335
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• 2001

In order to establish a whitefly control system using conventional groups of insecticide(carbamate, organophosphorus and pyrethroid insecticides), three alternative application methods were designed on the medium growth stage of cucumber. To discriminate the effectiveness of these sequences observed were the residual activity of insecticides, frequency of insecticide applications, residue of insecticides in cucumber leaves, development of insecticide resistance in whitefly, and yield of fruits. Spraying furathiocarb, a carbamate insecticide, was very effective in reducing the frequency of application for the control of white flies. The effectiveness of furathiocarb was enhanced by the potentiation process to carbofuran, the long residual activity, and the lower development rate of insecticide resistance. Methion, an organophosphorus insecticide, did not show resistance development after successive use but resulted in short residual activity. However, other organophosphates, profenofos and phenthoate, lost their activity by the resistance development. Decreasing activity was common to pyrethroids, deltamethrin and zetacypermethrin due to resistance. From these results it could be drawn a conclusion that furathiocarb, a carbamate insecticide, was the most desirable among conventional insecticide groups for the management of greenhouse whitefly population on the cucumber. To prevent an outbreak of the insect pest by various cause, it was recommended to choose acetamiprid, a nicotinoid, which showed very good control efficacy to the resistance insects to conventional insecticides.

• Korean journal of applied entomology
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• 제50권4호
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• pp.289-293
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• 2011

This study was conducted to develop economic injury level (EIL) of onion thrips, Thrips tabaci, on welsh onion (Allium fistulosum L. var) in the early transplanting stage. The changes of welsh onion biomass, yield loss, and T. tabaci density were investigated according to the inoculation periods of T. tabaci. In the early transplanting stage of welsh onion, the yield loss (%) increased with increasing inoculation periods: 17.0, 53.3, 38.4, and 80.8% yield loss in 5, 10, 15, and 20 d inoculation periods, respectively. The relationship between Cumulative Insect Days (CID) of T. tabaci and yield loss (%) of welsh onion was well described by a nonlinear logistic equation. Using the estimated equation, EIL of T. tabaci on welsh onion was estimated to 30 CID per plant based on the yield loss 12% (an empirical gain threshold 5% + marketable rate 93% of welsh onion). ET was calculated to 24 CID, which corresponds to 80% of EIL. Until a more defined EIL-model is developed, the present results should be useful for T. tabaci management in early growth stage of welsh onion. The effect of T. tabaci attack on the yield of welsh onion in late growing season (120 days after transplanting) was also examined. The yield of welsh onion increased at a low population density of T. tabaci and decreased at higher densities, showing a typical over-compensatory response.

• Korean Journal of Social Welfare
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• 제68권3호
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• pp.51-71
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• 2016

The purpose of this study is to clarify macro causes influencing on the diversity of single-mother households poverty among OECD Countries including Korea. This study carried out pooled time series cross-section analysis applying unbalanced panel design on the period from 1981 to 2012. There is marked diversity on single-mother households poverty. GDP per capita does not contributes to reduce poverty, and female employment rate and % population 0-14 exacerbate poverty. Several factors contribute on poverty reduction including social spending, child cash spending, union density, employment protection on regular workers, proportional representation system, cumulative left cabinet, cumulative women seat. In Korea, it needs to overcome the limit of anti-poverty strategy mainly based on economic growth and labor market flexibility. And it needs to enlarge universal welfare institutions, child benefits, work-family reconciliation policy, and to design adjusted labor market institutions including union density and employment protection, to introduce consensus political model including proportional representation system to enhance left power and women's representation.

• 대한공업교육학회지
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• 제45권1호
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• pp.84-102
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• 2020

During the period of rapid industrial growth, specialized high schools were important vocational education institutions trained specialists and technicians. But now, specialized high schools turned into high schools where the low-ranking students who were pushed out of the competition for admission due to a decline in the education population, changes mostly in labor demand in the labor market, and discrimination against skilled workers. In order to solve this problem, the middle school 3rd grade teachers in charge of the middle school can provide early career guidance, so that they can decide to go to the specialized high school according to their aptitude and talent rather than their grades. The purpose of this study is to investigate the relationship between general characteristics and perception of middle school 3rd grade teachers, and the rate of decision to enter specialized high school. In 2018, The middle school 3rd grade teachers in charge in Daejeon metropolitan city were selected. The results of this study were as follows: When 3rd grade teachers in charge are the male teachers 21.01%(M=.2101) of their students go to specialized high schools. When they are teachers who have not graduated from college of education(M=.2110), with doctoral degree(M=0.3064), of their students go to specialized hight schools. Finally, there was a significant positive relationship between perceptions of high school teachers (β1 = .245, p = .000) and high school graduates (β2 = .149, p = .027). The regression equation derived from this is y = -0.56 + 0.037 * β1 + 0.025 * β2 10.1% (R² = 0.101).

• Journal of Wetlands Research
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• 제21권3호
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• pp.244-250
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• 2019

Due to the industrial development and population growth, it has recently been shown that there are many problems caused by the rinked treatment water in local goverments and sewage treatment plants. The rinked treatment water has a characteristic of low flow rate and high concentration unlike general sewage. These characteristics increase sewage treatment difficulty and sewage treatment fee of sewage treatment facilities. Among the many influencing factors that increase sewage treatment unit cost, 'linked treatment load/design inflow load (%)' was derived as the most correlated factor. Through the selection and modeling of sewage treatment plants, the excess scope of design discharge water quality was investigated under the conditions of temperature and the conditions of 'linked treatment load/design inflow load (%)' taking into account the effects of the four seasons. The study found that for TN, 'linked treatment load/design inflow load (%)' was 19.7%, 22.6%, 25.1%and 27.7%, respectively, under conditions of $5^{\circ}C$, $10^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$. In case of TP, 'rinked treatment load/design inflow load (%)' was 10.7%, 12.2%, 15.6% and 17.5% at $5^{\circ}C$, $10^{\circ}C$, $20^{\circ}C$, and $25^{\circ}C$, respectively, under conditions of $5^{\circ}C$, $10^{\circ}C$, $20^{\circ}C$ and $25^{\circ}C$.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제22권2호
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• pp.667-675
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• 2021

The purpose of this study is as follows: First, to analyze the spatial structure of Jeju by analyzing the rate of parking demand and supply; Second, to determine the parking service imbalance between regions; and third, to elucidate the causes of this spatial structural parking service imbalance and provide effective measures. Due to the rapid population and tourism growth, Jeju is suffering from traffic congestion and serious parking problems. For this, the ways to efficiently utilize the existing parking spaces were identified through spatial structure analysis based on the parking demand and supply in the Jeju area. As short-term policy, the following changes were proposed: switching to small-scale eup or myeon blocks of parking space, use of shared parking system, and repairing side streets in residential areas. As mid-to-long-term policy, it is linked to other means of transportation as follows: parking demand management policy through modal share by reorganizing public transportation routes or expanding traffic facilities; securing park-and-ride lots; and different parking pricing for different time periods. To implement these policies, each area of the public sector, private sector, and private-public partnership should develop the bond of sympathy by areas and participants.

• Journal of Life Science
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• 제32권1호
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• pp.70-77
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• 2022

Endocrine disrupting chemicals (EDCs) have been attracting significant attention in modern society, owing to the increased incidence rate of various diseases along with population growth. EDCs are found in many commercial products, including some plastic bottles and containers, detergents, liners of metal food cans, flame retardants, food, toys, cosmetics, and pesticides. EDCs have a hormonal effect on the human body, which disrupts the endocrine system, notably affecting sexual differentiation and normal reproduction, and can trigger cancer as well. Recently, the association between neurological diseases and EDCs has become a hot topic of research in the field of neuroscience. Considering that EDCs negatively affect not only neuronal proliferation and neurotransmission but also the formation of the neuronal networks, EDCs may induce neurodevelopmental disorders, such as autism spectrum disorders and attention-deficit/hyperactivity disorder as well as neurodegenerative diseases, including Parkinson's disease and Alzheimer's disease. In light of these potentially deleterious outcomes, important efforts have been underway to minimize the exposure to EDCs through appropriate regulations and policies around the world, but chemicals that have not yet been associated with endocrine disrupting properties are still in wide use. Therefore, more epidemiological investigations and research are needed to fully understand the effects of EDCs on the nervous system.

• International conference on construction engineering and project management
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• The 9th International Conference on Construction Engineering and Project Management
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• pp.1247-1248
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• 2022

Due to rapid urbanization and population growth, it has become crucial to analyze the various volumes and characteristics of pedestrian pathways to understand the capacity and level of service (LOS) for pathways to promote a better walking environment. Different indicators have been developed to measure pedestrian volume. The pedestrian level of service (PLOS), tailored to analyze pedestrian pathways based on the concept of the LOS in transportation in the Highway Capacity Manual, has been widely used. PLOS is a measurement concept used to assess the quality of pedestrian facilities, from grade A (best condition) to grade F (worst condition), based on the flow rate, average speed, occupied space, and other parameters. Since the original PLOS approach has been criticized for producing idealistic results, several modified versions of PLOS have also been developed. One of these modified versions is perceived PLOS, which measures the LOS for pathways by considering pedestrians' awareness levels. However, this method relies on survey-based measurements, making it difficult to continuously deploy the technique to all the pathways. To measure PLOS more quantitatively and continuously, researchers have adopted computer vision technologies to automatically assess pedestrian flows and PLOS from CCTV videos. However, there are drawbacks even with this method because CCTVs cannot be installed everywhere, e.g., in alleyways. Recently, a technique to monitor bio-signals, such as electrodermal activity (EDA), through wearable sensors that can measure physiological responses to external stimuli (e.g., when another pedestrian passes), has gained popularity. It has the potential to continuously measure perceived PLOS. In their previous experiment, the authors of this study found that there were many significant EDA responses in crowded places when other pedestrians acting as external stimuli passed by. Therefore, we hypothesized that the EDA responses would be significantly higher in places where relatively more dynamic objects pass, i.e., in crowded areas with low PLOS levels (e.g., level F). To this end, the authors conducted an experiment to confirm the validity of EDA in inferring the perceived PLOS. The EDA of the subjects was measured and analyzed while watching both the real-world and virtually created videos with different pedestrian volumes in a laboratory environment. The results showed the possibility of inferring the amount of pedestrian volume on the pathways by measuring the physiological reactions of pedestrians. Through further validation, the research outcome is expected to be used for EDA-based continuous measurement of perceived PLOS at the alley level, which will facilitate modifying the existing walking environments, e.g., constructing pathways with appropriate effective width based on pedestrian volume. Future research will examine the validity of the integrated use of EDA and acceleration signals to increase the accuracy of inferring the perceived PLOS by capturing both physiological and behavioral reactions when walking in a crowded area.