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http://dx.doi.org/10.5352/JLS.2010.20.11.1634

Induction of Physiological Sex-Reversal and Gynogenetic Diploid in Rainbow Trout, Oncorhynchus mykiss  

Lee, Cheul-Ho (Cold-Water Fish Research Center, National Fisheries Research & Development Institute (NFRDI))
Kim, Dae-Jung (Inland Aquaculture Research Center, NFRDI)
Jeong, Chang-Hwa (Binex, Inc.)
Choi, Gyeong-Cheol (Chungcheongbuk-do Inland Fisheries Research Institute)
Lee, Chae-Sung (Cold-Water Fish Research Center, National Fisheries Research & Development Institute (NFRDI))
Kim, Dong-Soo (Department of Aquaculture, Pukyong National University)
Publication Information
Journal of Life Science / v.20, no.11, 2010 , pp. 1634-1639 More about this Journal
Abstract
This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 mg of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at $10^{\circ}C$ and $13^{\circ}C$. More than 93% of the male population was produced by this method and growth of hormone-treated fish at $13^{\circ}C$ was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least $3,600\;erg/cm^2$ was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at $28^{\circ}C$ for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.
Keywords
Rainbow trout; sex-reversal; gynogenetic diploid; 17 alpha-methyltestosterone;
Citations & Related Records
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