• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.652-658
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• 2011

The growth kinetics of Streptomyces noursei NRRL 5126 was investigated under different aeration and agitation combinations in a 5.0 l stirred tank fermenter. Poly-${\varepsilon}$-lysine biosynthesis, cell mass formation, and glycerol utilization rates were affected markedly by both aeration and agitation. An agitation speed of 300 rpm and aeration rate at 2.0 vvm supported better yields of 1,622.81 mg/l with highest specific productivity of 15 mg/l.h. Fermentation kinetics performed under different aeration and agitation conditions showed poly- ${\varepsilon}$-lysine fermentation to be a growth-associated production. A constant DO at 40% in the growth phase and 20% in the production phase increased the poly-${\varepsilon}$-lysine yield as well as cell mass to their maximum values of 1,992.35 mg/l and 20.73 g/l, respectively. The oxygen transfer rate (OTR), oxygen utilization rate (OUR), and specific oxygen uptake rates ($qO_2$) in the fermentation broth increased in the growth phase and remained unchanged in the stationary phase.

• 한국통신학회논문지
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• 제33권5C호
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• pp.395-402
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• 2008

본 논문에서는 무선 통신 분야의 WLAN/WMAN 시스템에 집적화할 수 있도록 I/Q 채널 12비트 40MS/s 파이프라인 아날로그-디지털 변환기를 제안하였다. 제안하는 A/D 변환기는 높아진 동작 속도와 CMOS 소자의 최소 선폭이 작아지며 생기는 듀티 사이클의 변화를 보정해 줄 수 있는 DLL 기반의 듀티 사이클 보정 회로를 집적화 하였다. 입력 듀티 사이클이 1%에서 99%까지 변동이 있어도 정확한 50%의 듀티 사이클을 가진 신호로 보정 가능하도록 설계하였다. 제작된 A/D 변환기는 $0.18{\mu}m$ CMOS n-well 1-poly 6-metal 공정으로 제작되었으며, 전력 소모는 1.8V 전원 전압에서 184mW이다. 샘플링 및 입력 주파수가 각각 20MHz, 1MHz 일 때 52dB의 SNDR과 59dBc의 SFDR을 나타내었다.

• 한국통신학회논문지
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• 제24권12A호
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• pp.2002-2007
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• 1999

본 논문에서는 유리기판 위에 고상결정화(SPC)로 제작된 n-채널 다결정 박막 트랜지스터(poly-Si TFT's)에 대해 전류-전압 특성, 이동도, 누설전류, 문턱전압, 그리고 부임계 기울기 등과 같은 전기적 특성을 측정함으로서 대면적, 고밀도 TFT-LCD에의 적용 가능성을 조사하였다. 채널 길이가 각각 2, 10, 25$\mu\textrm{m}$로 제작된 n-채널 poly-Si TFT에서, 전계 효과 이동도는 각각 11, 125, 116 $\textrm{cm}^2$/V-s이었으며, 누설전류는 각각 0.6, 0.1, 0.02 pA/$\mu\textrm{m}$로 나타났다. 또한 낮은 문턱전압과 q임계 기울기 그리고 양호한 ON-OFF ratio이 나타났다. 따라서, SPC로 제작된 poly-Si TFT는 대형유리기판에 디스플레이 패널과 구동시스템을 동시에 집적하는 대면적, 고밀도 TFT-LCD에 적용 가능한 것으로 판단된다.

• 폴리머
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• 제32권6호
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• pp.537-543
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• 2008

Crosslinked agar-g-poly(acrylic acid) (x-agar-g-PAA) super absorbent with a water absorbency ($Q_{H2O}$) of approximately 660 g/g was synthesized by the copolymerization of agar with an acrylic acid monomer. KPS and MBA were used as the initiator and crosslinker, respectively. Grafting was performed in air. Infrared spectroscopy was used to identify the product of copolymerization. The optimum conditions to synthesize the x-agar-g-PAA superabsorbent were 0.1 g of agar, 0.1 g of the KPS initiator, for 15 min; 50% AA monomer, 0.005 g of the MBA crosslinker, for a propagation time of 5 min; and 1 M NaOH for 15 min to allow for saponification. The reaction temperature was $80{^\circ}C$.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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• pp.1287-1290
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• 2006

A high speed analog buffer using polycrystalline silicon (poly-Si) thin film transistors (TFT) is proposed for 2.2-inch quarter video graphic adapter (qVGA) TFT-LCD panel. Simulation results show that the settling time of the proposed circuit is $10{\mu}sec$ in 2.2-inch qVGA and the power consumption of proposed analog buffer is $25{\mu}W$.

• Journal of Computing Science and Engineering
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• 제8권3호
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• pp.129-136
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• 2014

This paper presents the equivalent circuit modelling and eigenfrequency analysis of a wideband robust capacitive radio frequency (RF) microelectromechanical system (MEMS) switch that was designed using Poly-Si and Au layer membrane for highly reliable switching operation. The circuit characterization includes the extraction of resistance, inductance, on and off state capacitance, and Q-factor. The first six eigenfrequencies are analyzed using a finite element modeler, and the equivalent modes are demonstrated. The switch is optimized for millimeter wave frequencies, which indicate excellent RF performance with isolation of more than 55 dB and a low insertion loss of 0.1 dB in the V-band. The designed switch actuates at 13.2 V. The R, L, C and Q-factor are simulated using Y-matrix data over a frequency sweep of 20-100 GHz. The proposed switch has various applications in satellite communication networks and can also be used for devices that will incorporate the upcoming IEEE Wi-Fi 802.11ad protocol.

• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.471-478
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• 2014

Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the immune response of organism to RNA viruses. Quantitative real-time PCR (qRT-PCR) is a standard method for quantification of gene expression studies. However, the reliability of qRT-PCR data critically depends on proper selection of reference genes. In the study, using two different analysis programs, geNorm and NormFinder, we systematically evaluated the gene expression stability of six candidate reference genes (GAPDH, ACTB, B2M, RPL4, TBP, and PPIA) in samples of whole blood and PBMC with or without poly I:C stimulation. Generally, the six candidate genes performed a similar trend of expression stability in the samples of whole blood and PBMC, but more stably expressed in whole blood than in PBMC. geNorm ranked B2M and PPIA as the best combination for gene expression normalization, while according to NormFinder, TBP was ranked as the most stable reference gene, followed by B2M and PPIA. Comprehensively considering the results from the two programs, we recommended using the geometric mean of the three genes, TBP, PPIA and B2M, to normalize the gene expression of whole blood and PBMC with poly I:C stimulation. Our study is the first detailed survey of the gene expression stability in whole blood and PBMC with or without poly I:C stimulation and should be helpful for investigating the molecular mechanism involved in porcine whole blood and PBMC in response to poly I:C stimulation.

• 대한본초학회지
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• 제36권1호
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• pp.97-102
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• 2021

Objectives : Virus infection through the respiratory tract causes various inflammatory diseases such as pneumonia, cystic fibrosis, and obstructive pulmonary disease, causing enormous social damage. Therefore, it is very important to develop a treatment and prevention of infectious diseases. In this study, we investigated the effect of water extracts of Liriope muscari (WELM), known to improve lung function, on the inflammatory response of lung carcinoma cell line A549 cells induced by the viral double stranded RNA mimetic Polyinosinic:polycytidylic acid (Poly I:C). Methods : The cell viability by WELM treatment was analyzed using MTS assay in A549 cells. After inducing an inflammatory response to WELM-treated A549 cells with Poly I:C, the degree of apoptosis was confirmed through bright field microscopy. Interferon beta (IFN-β) mRNA expression level in A549 cells was analyzed by quantitative reverse transcription PCR (qRT-PCR). Results : WELM treatment has no significant effect on cell viability of A549 cells. We confirmed that pre-treatment of WELM effectively reduces the Poly I:C-induced apoptotic cell death in A549 cells. In addition, it was confirmed that the mRNA expression level of IFN-β, a pro-inflammatory cytokine increased by Poly I:C treatment, was significantly suppressed by WELM treatment in A549 cells. Conclusions : These results provide the evidence that WELM is effective at inhibiting inflammation on respiratory viral infections and suggest that Liriope muscari might be a valuable natural substance in the prevention and treatment of infectious diseases.

• Macromolecular Research
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• 제12권6호
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• pp.559-563
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• 2004

Both the ultrasonic velocity at 3 MHz and the absorption coefficient in the frequency range from 0.2 to 2 MHz were measured for aqueous solutions of poly(sodium 4-styrenesulfonate) over the concentration range from 5 to $25\%$ (by weight). The pulse echo overlap method was employed to measure the ultrasonic velocity over the temperature range from 10 to $90^{\circ}C;$ the high-Q ultrasonic resonator method was used for the measurement of the absorption coefficient at $20^{\circ}C.$ The velocities exhibited their maximum values at ca. 55, 59, 63, 67, and $71^{\circ}C.$ for the 25, 20, 15, 10, and $5\%$ solutions, respectively. The velocity increased with respect to the poly(sodium 4-styrene-sulfonate) concentration at a given temperature. A study of the concentration dependence of the both the relaxation frequency and amplitude indicated that the relaxation at ca. 200 kHz is related to structural fluctuations of the polymer molecules, such as the segmental motions of the polymer chains and that the relaxation at ca. 1 MHz resulted from the proton transfer reactions of the oxygen sites of $SO_3.$ Both the absorption and the shear viscosity increase upon increasing the polymer concentration, but they decrease upon increasing the temperature.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.693-699
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• 2010

An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa, as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and $60^{\circ}C$, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C> poly G>poly A>poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an $IC_50$ value of $8.6\;{\mu}M$ and $7.2\;{\mu}M$, respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.