[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.0911.11022

An Antiproliferative Ribonuclease from Fruiting Bodies of the Wild Mushroom Russula delica

Zhao, Shuang (State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University)
Zhao, Yong Chang (Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science)
Li, Shu Hong (Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science)
Zhang, Guo Qing (State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University)
Wang, He Xiang (State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University)
Ng, Tzi Bun (School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong)

Publication Information

Journal of Microbiology and Biotechnology / v.20, no.4, 2010 , pp. 693-699 More about this Journal

Abstract

An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa, as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and $60^{\circ}C$ , respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C> poly G>poly A>poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an $IC_50$ value of $8.6\;{\mu}M$ and $7.2\;{\mu}M$ , respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.

Keywords

Ribonuclease; Russula delica; antiproliferative; purification;

Citations & Related Records

Times Cited By Web Of Science : 4 (Related Records In Web of Science)

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