Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation |
Wang, Jiying
(Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences)
Wang, Yanping (Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences) Wang, Huaizhong (Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences) Hao, Xiaojing (Qingdao Institute of Animal Science and Veterinary Medicine) Wu, Ying (Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences) Guo, Jianfeng (Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences) |
1 | Caskey, M., F. Lefebvre, A. Filali-Mouhim, M. J. Cameron, J. P. Goulet, E. K. Haddad, G. Breton, C. Trumpfheller, S. Pollak, I. Shimeliovich, A. Duque-Alarcon, L. Pan, A. Nelkenbaum, A. M. Salazar, S. J. Schlesinger, R. M. Steinman, and R. P. Sekaly. 2011. Synthetic double-stranded RNA induces innate immune responses similar to a live viral vaccine in humans. J. Exp. Med. 208:2357-2366. DOI |
2 | Andersen, C. L., J. L. Jensen, and T. F. Orntoft. 2004. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64:5245-5250. DOI ScienceOn |
3 | Brym, P., A. Rusc, and S. Kaminski. 2013. Evaluation of reference genes for qRT-PCR gene expression studies in whole blood samples from healthy and leukemia-virus infected cattle. Vet. Immunol. Immunopathol. 153:302-307. DOI ScienceOn |
4 | Cao, Y., Z. Lu, Y. Li, P. Sun, D. Li, P. Li, X. Bai, Y. Fu, H. Bao, and C. Zhou. 2013. Poly (I: C) combined with multi-epitope protein vaccine completely protects against virulent foot-andmouth disease virus challenge in pigs. Antiviral Res. 97:145-153. DOI ScienceOn |
5 | Cinar, M. U., M. A. Islam, M. J. Uddin, E. Tholen, D. Tesfaye, C. Looft, and K. Schellander. 2012. Evaluation of suitable reference genes for gene expression studies in porcine alveolar macrophages in response to LPS and LTA. BMC Res. Notes 5:107. DOI |
6 | Facci, M. R., G. Auray, F. Meurens, R. Buchanan, J. van Kessel, and V. Gerdts. 2011. Stability of expression of reference genes in porcine peripheral blood mononuclear and dendritic cells. Vet. Immunol. Immunopathol. 141:11-15. DOI ScienceOn |
7 | Martino, A., M. Cabiati, M. Campan, T. Prescimone, D. Minocci, C. Caselli, A. M. Rossi, D. Giannessi, and S. Del Ry. 2011. Selection of reference genes for normalization of real-time PCR data in minipig heart failure model and evaluation of TNF- mRNA expression. J. Biotechnol. 153:92-99. DOI ScienceOn |
8 | Gao, Y., L. Flori, J. Lecardonnel, D. Esquerre , Z. L. Hu, A. Teillaud, G. Lemonnier, F. Lefevre, I. Oswald, and C. Rogel-Gaillard. 2010. Transcriptome analysis of porcine PBMCs after in vitro stimulation by LPS or PMA/ionomycin using an expression array targeting the pig immune response. BMC Genomics 11:292. DOI ScienceOn |
9 | Hellemans, J., G. Mortier, A. De Paepe, F. Speleman, and J. Vandesompele. 2007. qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol. 8:R19. DOI |
10 | Huggett, J., K. Dheda, S. Bustin, and A. Zumla. 2005. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 6:279-284. DOI ScienceOn |
11 | McCartney, S., W. Vermi, S. Gilfillan, M. Cella, T. L. Murphy, R. D. Schreiber, K. M. Murphy, and M. Colonna. 2009. Distinct and complementary functions of MDA5 and TLR3 in poly (I: C)-mediated activation of mouse NK cells. J. Exp. Med. 206:2967-2976. DOI ScienceOn |
12 | Meurens, F., A. Summerfield, H. Nauwynck, L. Saif, and V. Gerdts. 2012. The pig: a model for human infectious diseases. Trends Microbiol. 20:50-57. DOI ScienceOn |
13 | Nygard, A. B., C. B. Jorgensen, S. Cirera, and M. Fredholm. 2007. Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR. BMC Mol. Biol. 8:67. DOI |
14 | Pfaffl, M. W., A. Tichopad, C. Prgomet, and T. P. Neuvians. 2004. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26:509-515. DOI ScienceOn |
15 | Uddin, M. J., P. K. Nuro-Gyina, M. A. Islam, D. Tesfaye, E. Tholen, C. Looft, K. Schellander, and M. U. Cinar. 2012. Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide. Vet. Immunol. Immunopathol. 147:211-222. DOI ScienceOn |
16 | Radonic, A., S. Thulke, H.-G. Bae, M. A. Muller, W. Siegert, and A. Nitsche. 2005. Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections. Virol. J. 2:7. DOI ScienceOn |
17 | Spalenza, V., F. Girolami, C. Bevilacqua, F. Riondato, R. Rasero, C. Nebbia, P. Sacchi, and P. Martin. 2011. Identification of internal control genes for quantitative expression analysis by real-time PCR in bovine peripheral lymphocytes. Vet. J. 189:278-283. DOI ScienceOn |
18 | Uddin, M., M. Cinar, D. Tesfaye, C. Looft, E. Tholen, and K. Schellander. 2011. Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues. BMC Res. Notes 4:441. DOI ScienceOn |
19 | Vandesompele, J., K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, and F. Speleman. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:research0034- research0034.11. |
20 | Xiang-Hong, J., Y. Yan-Hong, X. Han-Jin, A. Li-long, X. Ying-Mei, J. Pei-Rong, and L. Ming. 2011. Selection of reference genes for gene expression studies in PBMC from Bama miniature pig under heat stress. Vet. Immunol. Immunopathol. 144:160-166. DOI ScienceOn |
21 | Zhang, J., Z. Tang, N. Wang, L. Long, and K. Li. 2012. Evaluating a set of reference genes for expression normalization in multiple tissues and skeletal muscle at different development stages in pigs using quantitative real-time polymerase chain reaction. DNA Cell Biol. 31:106-113. DOI ScienceOn |
22 | Cinar, M. U., M. A. Islam, M. Proll, H. Kocamis, E. Tholen, D. Tesfaye, C. Looft, K. Schellander, and M. J. Uddin. 2013. Evaluation of suitable reference genes for gene expression studies in porcine PBMCs in response to LPS and LTA. BMC Res. Notes 6:56. DOI |