• BMB Reports
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• 제36권3호
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• pp.275-281
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• 2003

An E1B-defective adenovirus, named r2/Ad carrying the neo expression cassette, was constructed by homologous recombination. The construction, selection (using neomycin as a selective marker), and propagation of the recombinant virus was performed in human embryonic kidney 293 cells (HEK 293). An in vitro study demonstrated that this recombinant virus has the ability to replicate in and lyse some p53-deficient human tumor cells such as human glioma tumor cells (U251) and human bladder cells (EJ), but not in some cells with functional p53, such as human adenocarcinoma cells (A549) and human fibroblast cells (MRC-5). Also, based on the cytopathic effect (CPE), it was demonstrated, under identical conditions, that the U251 cells were more sensitive to r2/Ad replication than the EJ cells. In this paper, we report that r2/Ad could be very useful in studying the in vitro selective replication of E1B-defective adenovirus and has great potential in cancer gene therapy.

• Journal of Electrical Engineering and Technology
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• 제12권6호
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• pp.2146-2156
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• 2017

When a failure occurs in active distribution system, it will be isolated through the action of circuit breakers and sectionalizing switches. As a result, the network might be divided into several connected components, in which distributed generations could supply power for customers. Aimed at decreasing customer interruption cost, this paper proposes a theoretically optimal island partition model for such connected components, and a simplified but more practical model is also derived. The model aims to calculate a dynamic island partition schedule during the failure recovery time period, instead of a static islanding status. Fluctuation and stochastic characteristics of the renewable distributed generations and loads are considered, and the interruption cost functions of the loads are fitted. To solve the optimization model, a heuristic search algorithm based on the hill climbing method is proposed. The effectiveness of the proposed model and algorithm is evaluated by comparing with an existing static island partitioning model and intelligent algorithms, respectively.

• 대한수학회보
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• 제44권1호
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• pp.13-29
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• 2007

In this paper, we first present a method for finding the implicit equation of the curve given by rational parametric equations. The method is based on the computation of $Gr\"{o}bner$ bases. Then, another method for implicitization of curve and surface is given. In the case of rational curves, the method proceeds via giving the implicit polynomial f with indeterminate coefficients, substituting the rational expressions for the given curve and surface into the implicit polynomial to yield a rational expression $\frac{g}{h}$ in the parameters. Equating coefficients of g in terms of parameters to 0 to get a system of linear equations in the indeterminate coefficients of polynomial f, and finally solving the linear system, we get all the coefficients of f, and thus we obtain the corresponding implicit equation. In the case of polynomial surfaces, we can similarly as in the case of rational curves obtain its implicit equation. This method is based on characteristic set theory. Some examples will show that our methods are efficient.

• Journal of Power Electronics
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• 제14권2호
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• pp.292-301
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• 2014

This paper presents a new configuration for a three-phase multilevel voltage source inverter. The main bridge is built from a classical three-phase two-level inverter and three bidirectional switches. A variable DC-link employing two unequal DC voltage supplies and four switches is connected to the main circuit in such a way that the proposed inverter produces four levels in the output voltage waveform. In order to obtain the desired switching gate signals, the fundamental frequency staircase modulation technique is successfully implemented. Furthermore, the proposed structure is extended and compared with other types of multilevel inverter topologies. The comparison shows that the proposed inverter requires a smaller number of power components. For a given number of voltage steps N, the proposed inverter requires N/2 DC voltage supplies and N+12 switches connected with N+7 gate driver circuits, while diode clamped or flying capacitor inverters require N-1 DC voltage supplies and 6(N-1) switches connected with 6(N-1) gate driver circuits. A prototype of the introduced configuration has been manufactured and the obtained simulation and experimental results ensure the feasibility of the proposed topology and the validity of the implemented modulation technique.

• Journal of Communications and Networks
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• 제16권2호
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• pp.162-171
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• 2014

Spectrum sensing is a key technical challenge for cognitive radio (CR). It is well known that multicycle cyclostationarity (MC) detection is a powerful method for spectrum sensing. However, a conventional MC detector is difficult to implement because of its high computational complexity. This paper considers reducing computational complexity by simplifying the test statistic of a conventional MC detector. On the basis of this simplification process, an improved MC detector is proposed. Compared with the conventional detector, the proposed detector has low-computational complexity and high-accuracy sensing performance. Subsequently, the sensing performance is further investigated for the cases of Rayleigh, Nakagami-m, Rician, and Rayleigh fading and lognormal shadowing channels. Furthermore, square-law combining (SLC) is introduced to improve the detection capability in fading and shadowing environments. The corresponding closed-form expressions of average detection probability are derived for each case by the moment generation function (MGF) and contour integral approaches. Finally, illustrative and analytical results show the efficiency and reliability of the proposed detector and the improvement in sensing performance by SLC in multipath fading and lognormal shadowing environments.

• Journal of Power Electronics
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• 제16권4호
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• pp.1551-1564
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• 2016

The high penetration level of inverter-based distributed generation (DG) power plants is challenging the low-voltage ride-through requirements, especially under unbalanced voltage sags. Recently, a flexible injection of both positive- (PS) and negative-sequence (NS) reactive currents has been suggested for the next generation of grid codes. This can enhance the ancillary services for voltage support at the point of common coupling (PCC). In light of this, considering distant grid faults that occur in a mainly inductive grid, this paper proposes a complete voltage support control scheme for the interface inverters of medium or high-rated DG power plants. The first contribution is the development of a reactive current reference generator combining PS and NS, with a feature to increase the PS voltage and simultaneously decrease the NS voltage, to mitigate voltage imbalance. The second contribution is the design of a voltage support control loop with two flexible PCC voltage set points, which can ensure continuous operation within the limits required in grid codes. In addition, a current saturation strategy is also considered for deep voltage sags to avoid overcurrent protection. Finally, simulation and experimental results are presented to validate the effectiveness of the proposed control scheme.

• Journal of Power Electronics
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• 제18권5호
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• pp.1303-1314
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• 2018

To match the dynamic lower voltage of a fuel cell stack and the required constant higher voltage (400V) of a DC bus, an H-type structural Boost three-level DC-DC converter with a wide voltage-gain range (HS-BTL) is presented in this paper. When compared with the traditional flying-capacitor Boost three-level DC-DC converter, the proposed converter can obtain a higher voltage-gain and does not require a complicate control for the flying-capacitor voltage balance. Moreover, the proposed converter, which can draw a continuous and low-rippled current from an input source, has the advantages of a wide voltage-gain range and low voltage stress for power semiconductors. The operating principle, parameters design and a comparison with other converters are presented and analyzed. Experimental results are also given to verify the aforementioned characteristics and theoretical analysis. The proposed converter is suitable for application of fuel cell systems.

• 경영과학
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• 제32권1호
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• pp.1-13
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• 2015

We estimate the option value embedded in reverse mortgages using the framework of European put option. The reverse mortgage is a very useful financial product for senior citizens who own homes but do not have a cash income while it is a high risk one from lender's perspective. One of benefits of the reverse mortgages is that the debt limit is restricted to the scope of the disposition price of the collateralized house, which is considered a put option to borrowers. The put option is evaluated using Black-Scholes model and a sensitive analysis is performed on variables such as discount rate, volatility, and time period. We confirm that the option value of reverse mortgages increases rapidly as the borrowers live longer than their life expectancy. The results of this study can be used to promote the reverse mortgage program more effectively in order to solve the problem of income shortage of the elderly homeowners.

• BMB Reports
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• 제45권11호
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• pp.665-670
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• 2012

The insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (PM), which facilitates food digestion and protects the gut epithelium. We used gel electrophoresis and mass spectrometry to identify the extracted proteins from the silkworm PM to obtain an in-depth understanding of the biological function of the silkworm PM components. A total of 305 proteins, with molecular weights ranging from 8.02 kDa to 788.52 kDa and the isoelectric points ranging from 3.39 to 12.91, were successfully identified. We also found several major classes of PM proteins, i.e. PM chitin-binding protein, invertebrate intestinal mucin, and chitin deacetylase. The protein profile provides a basis for further study of the physiological events in the PM of Bombyx mori.

• Journal of Electrical Engineering and Technology
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• 제10권4호
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• pp.1720-1725
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• 2015

The performance of the AlGaN/GaN MSM-2DEG varactor with different dielectric films deposited by the E-beam deposition is investigated in detail. The capacitance swing and the capacitance ratio of the varactor without dielectric film as well as with, SiO₂, Gd₂O₃, and Si₃N₄ films, respectively, are determined by electrodes of varying areas. The maximum capacitance, the minimum capacitance and the capacitance ratios are proportional to the increasing of the electrode areas. The capacitance ratio determined by the maximum and the minimum capacitance is found to be 18.35 (with Si₃N₄ dielectric film) and 149.51 (without dielectric film), respectively. The transition voltages of the fabricated varactors are almost the same for a bias voltage of about ±5 V and leakage current can be lower three orders of magnitude while the varactors with dielectric films. The tunability of the capacitance ratio makes the AlGaN/GaN MSM-2DEG varactor with a dielectric film highly useful in multirange applications of a surge free preamplier.