• The Korean Journal of Mycology
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• v.26 no.4 s.87
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• pp.466-477
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• 1998

Twenty-two Phellinus strains were characterized using colony morphologies and polymerase chain reaction (PCR) to divide into Phellinus linteus. There were some differences in mycelial growth and colony shapes among the strains when they were grown on various media such as PDA, MCM, MEA and YM. Phellinus linteus was slowly growing, formed golden-yellow colony, and produced blue pigment on PDA media. When the regions of internal transcribed spacer (ITS) were amplified from ribosomal RNA (rRNA) coding genes of P. igniarius and P. linteus strains by means of PCR, two types of band (700 bp and 800 bp) were appeared, respectively. For the amplified intergenic region I (IGRI), P. igniarius strains showed a different band among 500, 600, 700 and 800 bp according to the strains, whereas P. linteus strains did one specific band of 700 bp. By polymorphism analysis after digesting the amplified products with 6 different restriction enzymes, a band specific to P. linteus was generated when the products for ITS region were digested with HaeIII, suggesting that the enzyme digestion could provide effective method to distinguish between P. igniarius and P. linteus. And also, the analysis of genetic relationship showed that the genetic similarities were 89% and 95% in P. igniarius and P. linteus strains, respectively. Random amplification polymorphic DNA (RAPD) analysis using multiple primer sets and arbitrarily primed PCR (AP-PCR) with ITS3 primer could also result in a reproducible way to identify P. linteus strains.

• Journal of Photoscience
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• v.9 no.2
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• pp.421-423
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• 2002

Phellinus linteus mycelia have many pharmacological effects, although their pharmacological efficacy principles have not been known yet. In the course of screening for biological activity of the extracts of Phellinus linteus mycelia, we found strong antioxidative activity in some fraction of water-insoluble. Therefore, we tried to isolate the active principle(s) from the extract. The isolation of the active compound was guided by superoxide anion radical scavenging activity. As a result, caffeic acid was isolated as an active compound. The IC$\_$50/ of the compound was 3.05 $\mu$g/ml (16.9$\mu$M).

• Microbiology and Biotechnology Letters
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• v.28 no.5
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• pp.298-302
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• 2000

Fermentation parameters were estimated by use of a vent gas analyzer coupled to a computer data acquisition system in cultivation of Phellinus linteus WI-001, pro-ducer of polysaccharides known to have potent anticancer activities. Oxygen uptake rate(OUR), a critical indicator of the cells activities, was calculated by applying oxygen mass balance. In addition, by dividing the oxygen uptake rate hy the total oxygen consumed, on-line estimation of the cells specific growth rate was successfully done. It was also possible to estimate cell concentration directly bt use of oxygen-cell yield($Y_{x/o}$ ) which was obtained based on a correlation between cell growth and total oxygen consumed.

• YAKHAK HOEJI
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• v.46 no.3
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• pp.174-178
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• 2002

Phellinus linteus (Hymenocaetaceae) is a member of Basidiomycotinae, which has been used for the treatment of gastric cancer, non-insulin-dependent diabetes mellitus, diarrhea, and menstrual irregularity. To investigate the diuretic action of Phellinus linteus, urinary volume, serum parameters and urinary electrolytes in HgCl$_2$-induced acute renal failure rats in vivo were measured. The results showed that its MeOH extract produced significant increases on urinary volume, sodium and potassium excretion, and revealed lowering effects of the abnormally elevated BUN, creatinine and uric acid value in acute renal failure rats.

• Journal of Applied Biological Chemistry
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• v.53 no.3
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• pp.179-183
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• 2010

To produce the functional food materials from edible mushrooms, hot-water extracts from 70 kinds of mushroom mycelia were examined for anti-complementary activity and Phellinus linteus showed the highest activity through the complement fIxation test. The maximum production of Phellinus linteus mycelia with anti-complementary activity was observed in culture medium containing soluble starch 3.0%, peptone 0.3%, yeast extract 0.4%, $MgSO_4{\cdot}7H_2O$ 0.1%, $K_2HPO_4$ 0.2% and in the culture conditions controlled at initial pH 7.0, $30^{\circ}C$ and 150 rpm by the rotary shaker. In addition, the maximum production of mycelial dry weight was 15 mg/mL after 18 days under the optimal conditions, and anti-complementary activity was reached to 88% in 5 L-jar fermenter.

• Mycobiology
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• v.36 no.2
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• pp.81-87
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• 2008

The optimal conditions for mycelial growth of Phellinus linteus ATCC 26710 were determined to be a log length of 20 cm, temperature of $30^{\circ}C$ and pH of 6.0. Mycelial growth was excellent on the mushroom complete medium, and was optimal when sucrose, man nose and glucose were supplied as carbon sources. Potassium nitrate and sodium nitrate as nitrogen sources supported good mycelial growth. To evaluate P. linteus mycelial colonization on logs, sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation techniques were used. Only sterilized short log inoculation produced good mycelial colonization. Initial mycelial growth and full mycelial colonization were best on 20 cm logs having 42% moisture content. The initial mycelial growth of P. linteus was accelerated over 12hr of sterilization. Basidiocarp formation was optimal using a burying method of logs after $5{\sim}6$ months, and fruiting body formation was superior in cultivation house conditions of $31{\sim}35^{\circ}C$ and in excess of 96% relative humidity.

• Mycobiology
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• v.30 no.4
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• pp.208-212
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• 2002

For comparison of mycelial colonization of Phellinus linteus on logs, several techniques of inoculation were tested; sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich inoculation. The initial mycelial growth and the full mycelial colonization of P. linteus in logs were the best in case of 20 cm logs under the condition of 42% moisture content. Also, the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after $5{\sim}6$ months of incubation was the best for formation of basidiocarps of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at $31{\sim}35^{\circ}C$ and over 96% of relative humidity.

• Journal of Environmental Science International
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• v.21 no.9
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• pp.1087-1095
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• 2012

The investigation was made about distribution and ecological characteristics of host plant for Phellinus linteus at habitats in Gangwon-Do. The habitats of P. linteus are the place where the fog is much generated and there is lots of the moisture. The flora of the vascular plants in P. linteus habitats were consisted of 76 taxa; 62 species, 10 varieties and 4 formas of 62 genera of 40 families. The plants of infiltration type were found 70% around P. linteus habitats. This results shows that the natural environments of P. linteus habitat is very stable condition. The categories of vegetation were classified into two types. The host plant for P. linteus appeared 61.6% from Populus tomentiglandulosa. The first type showed up above the sea about 600m and west exposure region. The second type was investigated around the facing north region of the steep slope-land.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.1
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• pp.71-77
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• 2010

The purpose of the present study was to examine the effect of water extracts from Phellinus linteus on lipid composition and antioxidative system in rats fed high fat high cholesterol diet. Rats were divided into four experimental groups which are composed of normal diet group, high fat high cholesterol diet group, high fat high cholesterol diet with 50 mg/kg b.w water extracts from Phellinus linteus supplemented group (PA group), high fat high cholesterol diet with 100 mg/kg b.w water extracts from Phellinus linteus supplemented group (PB group). The serum TG and cholesterol contents of the water extracts from Phellinus linteus supplemented groups (PA and PB groups) were decreased compared to the high fat high cholesterol diet group. The serum HDL-cholesterol contents of the water extracts from Phellinus linteus supplemented groups were increased compared to the high fat high cholesterol diet group. The serum LDL-cholesterol and AI of the water extracts from Phellinus linteus supplemented groups were significantly decreased compared to the high fat high cholesterol diet group. Supplementation of water extracts from Phellinus linteus groups (PA and PB groups) resulted in increased activities of superoxide dismutase. Hepatic glutathione peroxidase (GSH-px) were increased compared to the high fat high cholesterol diet group. TBARS values in liver and plasma were reduced in water extracts from Phellinus linteus supplemented groups. These result suggest that supplementation of water extracts from Phellinus linteus may have a pronounced impact on lipid composition and antioxidative system in the rats fed high fat high cholesterol diet.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.4
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• pp.431-440
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• 2011

This experiment was conducted to investigate the effects of molasses and $Phellinus$ $linteus$ meal supplementation on the quality of Korean herbal medicine silage. Herbal medicine meal silages were produced by the addition of 0, 15, and 30% of $Phellinus$ $linteus$ meal and 0, 1, and 2% of molasses ($3{\times}3$ factorial design) and stored for 40 days at room temperature. There were three replicates per treatment. Its quality such as chemical composition, pH, organic acids, the number of microorganisms and $in$ $vitro$ dry matter disappearance were determined. As the $Phellinus$ $linteus$ meal level increased, crude protein and crude fat contents increased significantly, but the acid detergent fiber (ADF) content decreased significantly. As the addition of $Phellinus$ $linteus$ meal decreased, and molasses increased, pH tended to decrease, but the lactic acid content trended to increase. The acetic acid content was lower in a higher level (30%) of $Phellinus$ $linteus$ meal, and higher in a higher level (2%) of molasses. As the $Phellinus$ $linteus$ meal level increased, the butyric acid content decreased, and butyric acid of molasses treatment was higher than that of non-supplemented control. Molasses increased the number of Lactobacillus, but decreased the number of fungi in silage. A lower level of $Phellinus$ $linteus$ meal increased the number of Lactobacillus, but decreased the number of fungi. $in$ $vitro$ dry matter disappearance tended to increase by the addition of $Phellinus$ $linteus$ meal and molasses. It is concluded that the addition of molasses and $Phellinus$ $linteus$ to herbal medicine meal silage could enhance its nutritional quality. It is considered that proper levels of molasses and $Phellinus$ $linteus$ meal in herbal medicine meal silage were 2 % and 15% respectively.